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Preparation Of Liposomes Of Defined Size Distribution By Extrusion Through Polycarbonate Membranes.
F. Olson, C. A. Hunt, F. Szoka, W. Vail, D. Papahadjopoulos
Published 1979 · Materials Science, Medicine
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Liposomes of defined size and homogeneity have been prepared by sequential extrusion of the usual multilamellar vesicles through polycarbonate membranes. The process is easy, reproducible, produces no detectable degradation of the phospholipids, and can double the encapsulation efficiency of the liposome preparation. Multilamellar vesicles extruded by this technique are shown by both negative stain and freeze-fracture electron microscopy to have mean diameters approaching the pore diameter of the polycarbonate membrane through which they were extruded. When sequentially extruded down through a 0.2 micron membrane, the resulting vesicles exhibit a very homogeneous size distribution with a mean diameter of 0.27 micron while maintaining an acceptable level of encapsulation of the aqueous phase.
This paper references
Large volume liposomes by an ether vaporization method.
D. Deamer (1976)
Retention of cytosine arabinoside in mouse lung following intravenous administration in liposomes of different size.
C. A. Hunt (1979)
BBA reportLarge volume liposomes by an ether vaporization method
D. Deamer (1976)
Membrane models with phospholipids.
A. Bangham (1968)
Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles.
D. Papahadjopoulos (1975)
Phase transitions in phospholipid vesicles. Fluorescence polarization and permeability measurements concerning the effect of temperature and cholesterol.
D. Papahadjopoulos (1973)
Phosphatidylcholine vesicles. Formation and physical characteristics
C. Huang (1969)
The effect of particle size on blood clearance and tissue distribution of radioactive gold colloids.
D. B. Zilversmit (1952)
Electron microscopic examination of subcellular fractions. II. Quantitative analysis of the mitochondrial population isolated from rat liver.
P. Baudhuin (1967)
The effect of particle size and charge on the clearance rates of liposomes and liposome encapsulated drugs.
R. Juliano (1975)
Differential distribution of liposome-entrapped [3H]methotrexate and labelled lipids after intravenous injection in a primate.
H. Kimelberg (1976)
Diffusion of univalent ions across the lamellae of swollen phospholipids.
A. Bangham (1965)
The carrier potential of liposomes in biology and medicine (second of two parts).
G. Gregoriadis (1976)
A simple method for the preparation of homogeneous phospholipid vesicles.
Y. Barenholz (1977)
New aspects of liposomes.
D. A. Tyrrell (1976)
Phospholipid model membranes. I. Structural characteristics of hydrated liquid crystals.
D. Papahadjopoulos (1967)
The carrier potential of liposomes in biology and medicine (first of two parts).
G. Gregoriadis (1976)
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T. X. Xiang (1995)
Lipogastrins as potent inhibitors of viral fusion.
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Light-scattering properties of osmotically active liposomes
W. Yoshikawa (1983)
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Accurate Methodology for Monitoring Biomembrane Events
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Structural and motional properties of vesicles as revealed by nuclear magnetic resonance.
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