Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

A Novel Method For Encapsulation Of Macromolecules In Liposomes.

R. Shew, D. Deamer
Published 1985 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Hemoglobin and alkaline phosphatase were each encapsulated in phosphatidylcholine liposomes using a dehydration-rehydration cycle for liposome formation. In this method, liposomes prepared by sonication are mixed in aqueous solution with the solute desired to be encapsulated and the mixture is dried under nitrogen in a rotating flask. As the sample is dehydrated, the liposomes fuse to form a multilamellar film that effectively sandwiches the solute molecules. Upon rehydration, large liposomes are produced which have encapsulated a significant fraction of the solute. The optimal mass ratio of lipid to solute is approx. 1:2 to 1:3. This method has potential application in large-scale liposome production, since it depends only on a controlled drying and rehydration process, and does not require extensive use of organic solvents, detergents, or dialysis systems.
This paper references



This paper is referenced by
10.1089/ast.2016.1610
Can Life Begin on Enceladus? A Perspective from Hydrothermal Chemistry
D. Deamer (2017)
10.1016/0031-6865(95)00010-7
Preparation and characterization of liposomes as therapeutic delivery systems: a review.
S. Vemuri (1995)
10.15436/2377-1372.16.1139
Effect of Lyophilization on the Size and Polydispersity of Unilamellar and Multilamellar Liposomes
Mehdi Shokri (2016)
10.1007/S10847-006-9062-9
Prolonged Retention of Doxorubicin in Tumor Cells by Encapsulation of γ-Cyclodextrin Complex in Pegylated Liposomes
Yoshiyuki Hagiwara (2006)
10.1021/BI00375A004
Multilayered vesicles prepared by reverse-phase evaporation: liposome structure and optimum solute entrapment.
C. Pidgeon (1987)
10.1016/0378-5173(95)04227-X
Preparation and stability of interferon-α-containing liposomes
C. Karau (1996)
10.1016/S0167-7799(02)01909-1
Artificial cells: prospects for biotechnology.
A. Pohorille (2002)
10.1016/0378-5173(93)90211-W
Conductivity measurement as a convenient technique for determination of liposome capture volume
D. Lidgate (1993)
10.1016/j.biochi.2016.07.008
Nano-formulations of drugs: Recent developments, impact and challenges.
J. Jeevanandam (2016)
10.1081/DDC-120021771
Double Liposomes: Hypoglycemic Effects of Liposomal Insulin on Normal Rats
Ken Katayama (2003)
10.1007/s11084-007-9065-6
The Influence of Environmental Conditions, Lipid Composition, and Phase Behavior on the Origin of Cell Membranes
J. Thomas (2007)
10.1007/s00232-002-1046-0
Liposome-entrapped Polymerases as Models for Microscale/Nanoscale Bioreactors
P.-A. Monnard (2003)
10.1007/1-4020-2522-X_6
Prebiotic Amphiphilic Compounds
D. Deamer (2004)
10.1039/C7TB01495C
A tumour microenvironment-responsive polymeric complex for targeted depletion of tumour-associated macrophages (TAMs).
Y. Wang (2017)
10.1016/J.JCONREL.2005.08.023
Liposomalization of SN-38 as active metabolite of CPT-11.
Y. Sadzuka (2005)
10.1016/B978-0-7506-1494-8.50015-8
Chapter 10 – Production of vesicles of defined size
M. J. Lawrence (1994)
10.1016/0378-5173(93)90422-C
Preparation of liposomes using a Mini-Lab 8.30 H high-pressure homogenizer
D. Bachmann (1993)
10.1007/s11084-007-9098-x
Question 5: On the Chemical Reality of the RNA World
D. Lucrezia (2007)
Lateral Diffusion of Phospholipids Measured using 31P Centreband-only Detection of Exchange Nuclear Magnetic Resonance
A. Lai (2012)
Effets de biomolécules sur la diffusion de sondes fluorescentes dans des milieux organisés
A. Naoumi (1990)
10.1016/j.jconrel.2013.01.036
Liposomal surface coatings of metal stents for efficient non-viral gene delivery to the injured vasculature.
Sandra Ganly (2013)
10.1007/s11084-007-9111-4
Steps Towards the Formation of A Protocell: The Possible Role of Short Peptides
M. Fishkis (2007)
10.1039/c7ib00138j
Do protocells preferentially retain macromolecular solutes upon division/fragmentation? A study based on the extrusion of POPC giant vesicles.
A. Fanti (2018)
10.1016/0005-2736(88)90351-3
The captured volume of multilamellar vesicles.
W. Perkins (1988)
10.1039/C2PY20253K
A novel amphiphilic copolymer poly(ethylene oxide-co-allyl glycidyl ether)-graft-poly(ε-caprolactone): synthesis, self-assembly, and protein encapsulation behavior
B. Li (2012)
10.1016/J.TIBTECH.2005.05.008
A giant step towards artificial life?
D. Deamer (2005)
10.1016/S0076-6879(89)71012-0
[9] Liposome preparation and size characterization
M. Woodle (1989)
10.1021/bp0201004
Effective Encapsulation of Proteins into Size‐Controlled Phospholipid Vesicles Using Freeze‐Thawing and Extrusion
K. Sou (2003)
10.1007/b136806
Chemistry and Physics of Primitive Membranes
D. Deamer (2006)
10.3109/03639048909052502
Liposomes as a Drug Delivery System
N. D. Weiner (1989)
10.1039/c2cs35042d
Liquid crystalline nanostructures: organizing matrices for non-enzymatic nucleic acid polymerization.
D. Deamer (2012)
10.1038/srep31285
Organization of Nucleotides in Different Environments and the Formation of Pre-Polymers
Sebastian Himbert (2016)
See more
Semantic Scholar Logo Some data provided by SemanticScholar