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Microelectrophoretic Study Of Radiation-induced DNA Damages In Individual Mammalian Cells.

O. Ostling, K. Johanson
Published 1984 · Chemistry, Medicine

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Mammalian cells were after irradiation suspended in melted agarose, and casted on microscope slides. The slides were after gelling at 0 degree C immersed in a neutral detergent solution which lysed the cells. A weak electric field (5 V/cm) was then applied over the gel for 5 minutes. The DNA in the gel was stained with the fluorescent dye acridine orange and gives a green emission in a microscope photometer. DNA had migrated towards the anode and this migration was more pronounced in irradiated than in control cells. The differences in migration pattern were quantitatively measured. The lower detection limit was below 0.5 Gy and a plateau in the dose-effect curve was reached at about 3 Gy. In repair experiments residual DNA damage could be observed after postirradiation incubation for 60 minutes. The advantages of the method is: no radioactive labelling and only a few number of cells is required.
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This paper is referenced by
10.3109/02841868709092977
DNA damage in clinical radiation therapy studied by microelectrophoresis in single tumour cells. A preliminary report.
O. Ostling (1987)
10.1002/yea.2901
Mutagenic effect of freezing on nuclear DNA of Saccharomyces cerevisiae
T. Todorova (2012)
10.1016/0304-3835(94)90388-3
Laser scanning microscopic analysis of DNA damage in frozen tissues.
D. Fairbairn (1994)
10.1002/(SICI)1098-2280(1996)28:2<112::AID-EM7>3.0.CO;2-H
Alkaline single‐cell gel (comet) assay and genotoxicity monitoring using two species of tadpoles
S. Ralph (1996)
10.1002/(SICI)1098-2280(1996)27:1<39::AID-EM6>3.0.CO;2-9
Radiation‐induced DNA damage in canine hemopoietic cells and stromal cells as measured by the comet assay
L. Kreja (1996)
10.1016/S0027-5107(96)00241-2
Reproducibility of human sperm DNA measurements using the alkaline single cell gel electrophoresis assay.
C. Hughes (1997)
10.1016/S0921-8777(97)00007-4
Detection of MNNG-induced DNA lesions in mammalian cells; validation of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations.
D. Slameňová (1997)
10.1002/jgm.890
Radioprotective gene therapy through retroviral expression of manganese superoxide dismutase
T. Southgate (2006)
10.1093/NAR/26.11.2644
DNA repair defect in poly(ADP-ribose) polymerase-deficient cell lines.
C. Trucco (1998)
10.1002/cyto.a.20071
Single‐cell gel electrophoresis assay monitors precise kinetics of DNA fragmentation induced during programmed cell death
S. Chandna (2004)
10.1002/0471140856.tx0312s65
Alkaline Comet Assay for Assessing DNA Damage in Individual Cells
X. Pu (2015)
10.1093/MUTAGE/14.5.497
Processing of DNA damage induced by hydrogen peroxide and methyl methanesulfonate in human lymphocytes: analysis by alkaline single cell gel electrophoresis and cytogenetic methods.
C. Andreoli (1999)
10.1016/S1011-1344(00)00149-4
Hydrogen peroxide is responsible for UVA-induced DNA damage measured by alkaline comet assay in HaCaT keratinocytes.
A. B. Petersen (2000)
10.1182/BLOOD.V100.1.224
Repair of DNA interstrand crosslinks as a mechanism of clinical resistance to melphalan in multiple myeloma.
V. Spanswick (2002)
Induction of DNA double-strand breaks at various stages of the cell cycle using the comet assay
A. Mahmoud (2002)
10.1656/1092-6194(2002)009[0203:GIARSB]2.0.CO;2
GENOTOXICITY IN ANDROSCOGGIN RIVER SMALLMOUTH BASS
Kim Chamberland (2002)
10.1016/S0047-6374(03)00125-8
Age-related effects on induction of DNA strand breaks by intermittent exposure to electromagnetic fields
S. Ivancsits (2003)
10.1002/EM.20040
DNA damage produced in HaCaT cells by combined fluoranthene exposure and ultraviolet A irradiation
Baoying Zheng (2004)
10.1007/S00244-003-2253-0
The Comet Assay as Biomarker of Heavy Metal Genotoxicity in Earthworms
S. Reinecke (2004)
10.1016/S1383-5718(03)00182-7
DNA damage in zebrafish larvae induced by exposure to low-dose rate gamma-radiation: detection by the alkaline comet assay.
R. B. Jarvis (2003)
Programa de Pós-Graduação em Biotecnologia
L. A. Savi (2004)
10.1016/J.MRGENTOX.2003.10.013
Evaluation of DNA damage in a population of bats (Chiroptera) residing in an abandoned monazite mine.
K. A. Meehan (2004)
Fréquence et réparation de dommages à l'ADN associés à la 4-(méthylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk), une nitrosamine spécifique du tabac, évalués à l'aide du test des comètes
S. Lacoste (2007)
10.3746/JKFN.2007.36.10.1229
Antioxidant and Antigenotoxic Activities of Extracts from Anglerfish
이석희 (2007)
10.1201/9781420046359-c39
Food Irradiation and Its Detection
Y. Wong (2009)
10.1016/j.toxlet.2009.07.003
Genotoxicity of sodium arsenite and DNA fragmentation in ovarian cells of rat.
Zertashia Akram (2009)
10.1002/9780470622728.CH12
In Vivo Genotoxicity Assays
V. Thybaud (2010)
10.3746/JKFN.2010.39.9.1243
마전자 추출물의 항산화 및 Alpha-Glucosidase 저해 활성
이정민 (2010)
An integrated ecotoxicological assessment of the engineered nanoparticles, C60 fullerenes in different life stages of marine mussels, Mytilus edulis
Sherain N. Al-Subiai (2011)
10.1007/s00204-011-0682-5
DNA adducts and strand breaks in workers exposed to vapours and aerosols of bitumen: associations between exposure and effect
Boleslaw Marczynski (2011)
10.2174/156800911794519761
Peptidyl prolyl isomerase, Pin1 is a potential target for enhancing the therapeutic efficacy of etoposide.
R. Mathur (2011)
10.1016/j.mrgentox.2011.01.006
A modified protocol for the comet assay allowing the processing of multiple samples.
Lai-Jun Zhang (2011)
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