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Prostaglandin F2α Inhibits The Differentiation Of Adipocyte Precursors In Primary Culture

G. Serrero, N. Lepak, S. Goodrich
Published 1992 · Biology

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Abstract Influence of arachidonate metabolite pathway on adipose differentiation was investigated using primary culture of adipocyte precursors in defined medium. Treatment of the cells with cyclooxygenase inhibitors stimulates adipose differentiation by at least 2-fold. Among the various arachidonate metabolites tested, only prostaglandin F2α (PGF2α) was found to inhibit the differentiation of adipocyte precursors in a dose dependent fashion. Other eicosanoids tested did not have any effect. A 50% inhibition of adipose differentiation was observed with a dose of PGF2α of 3×10 −9 M to 7×10 −9 M according to the strain of rats used. Maximal inhibition occurred at PGF2α concentrations equal or higher than 10 −8 M. PGF2α inhibited not only the expression of late markers of adipose differentiation such as G3PDH and triglycerides accumulation but also the mRNA expression of early markers of adipose differentiation such as clone 154, lipoprotein lipase and ap2 gene. These results indicate that PGF2α represents a physiological negative modulator of adipose differentiation.
This paper references
10.1042/BJ2570399
Prostacyclin as a potent effector of adipose-cell differentiation.
R. Négrel (1989)
10.1128/MCB.7.12.4464
The synthesis of ribosomal proteins S16 and L32 is not autogenously regulated during mouse myoblast differentiation.
L. Bowman (1987)
10.1016/0005-2760(70)90148-7
The biosynthesis and possible function of prostaglandins in adipose tissue
E. Christ (1970)
10.1016/S0006-291X(77)80180-0
Differentiation of 3T3-L1 fibroblasts to adipocytes. The effect of indomethacin, prostaglandin E1 and cyclic AMP on the process of differentiation.
I. Williams (1977)
10.1146/ANNUREV.PA.19.040179.002345
Nonsteroidal anti-inflammatory agents.
R. Nickander (1979)
10.1126/SCIENCE.877563
Adipose tissue regeneration following lipectomy.
I. Faust (1977)
10.1016/0005-2760(81)90086-2
Differentiation of ob 17 preadipocytes to adipocytes. Effects of prostaglandin F2alpha and relationship to prostaglandin synthesis.
R. Négrel (1981)
10.1016/0003-2697(87)90021-2
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
10.1093/JN/119.3.458
Evidence for abnormal prostaglandin synthesis in obese Zucker rat adipose cell cultures.
H. Gaskins (1989)
Evidence for inhibition of leukotriene A4 synthesis by 5,8,11,14-eicosatetraynoic acid in guinea pig polymorphonuclear leukocytes.
G. Bokoch (1981)
10.1016/0006-291X(86)91165-4
Coupling of growth arrest and expression of early markers during adipose conversion of preadipocyte cell lines.
E. Amri (1986)
Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells.
L. S. Wise (1979)
10.1016/S0006-291X(72)80174-8
Relationship between inhibition of prostaglandin synthesis and drug efficacy: support for the current theory on mode of action of aspirin-like drugs.
R. V. Tomlinson (1972)



This paper is referenced by
10.1210/ENDO.131.6.1446597
Paracrine regulation of adipose differentiation by arachidonate metabolites: prostaglandin F2 alpha inhibits early and late markers of differentiation in the adipogenic cell line 1246.
G. Serrero (1992)
10.1016/0090-6980(93)90070-N
Inhibition of adipose differentiation by 9α, 11β-prostaglandin F2α
N. Lepak (1993)
Prostaglandin F,, Stimulates Transforming Growth Factor-a Expression in Adipocyte Precursors*
N. Lepak (1995)
10.1146/ANNUREV.CELLBIO.13.1.231
Adipocyte differentiation and leptin expression.
Chang Sun Hwang (1997)
10.1042/BJ20030503
Adipocyte differentiation of 3T3-L1 preadipocytes is dependent on lipoxygenase activity during the initial stages of the differentiation process.
L. Madsen (2003)
10.1016/S0090-6980(02)00134-X
Prostaglandin D2 synthase enzymes and PPARgamma are co-expressed in mouse 3T3-L1 adipocytes and human tissues.
I. R. Jowsey (2003)
10.1385/ABAB:118:1-3:133
Regulation and role of arachidonate cascade during changes in life cycle of adipocytes
S. Lu (2004)
Influencia endocrina y paracrina sobre la adipogénesis
A. Cortés (2002)
10.1016/J.BBALIP.2006.03.017
Gene expression of arachidonate cyclooxygenase pathway leading to the delayed synthesis of prostaglandins E2 and F2alpha in response to phorbol 12-myristate 13-acetate and action of these prostanoids during life cycle of adipocytes.
L. Xu (2006)
10.1101/836288
Inhibiting the prostaglandin transporter PGT induces non-canonical thermogenesis at thermoneutrality
Victor J Pai (2019)
10.1111/j.1463-1326.2005.00472.x
Differentiation‐dependent regulation of the cyclooxygenase cascade during adipogenesis suggests a complex role for prostaglandins
Y. Xie (2006)
10.1016/S1388-1981(00)00128-1
Adipose differentiation related protein: expression, purification of recombinant protein in Escherichia coli and characterization of its fatty acid binding properties.
G. Serrero (2000)
10.1016/S0898-6568(00)00137-6
Prostaglandin F2alpha enhances glucose consumption through neither adipocyte differentiation nor GLUT1 expression in 3T3-L1 cells
J. Kamon (2001)
10.1006/BBRC.1997.6433
Prostaglandin F2alpha receptor (FP receptor) agonists are potent adipose differentiation inhibitors for primary culture of adipocyte precursors in defined medium.
G. Serrero (1997)
10.1194/JLR.M300192-JLR200
Arachidonic acid-dependent inhibition of adipocyte differentiation requires PKA activity and is associated with sustained expression of cyclooxygenases Published, JLR Papers in Press, August 16, 2003. DOI 10.1194/jlr.M300192-JLR200
R. Petersen (2003)
10.1002/(SICI)1097-4652(200002)182:2<297::AID-JCP19>3.0.CO;2-Z
Stimulation of adipose differentiation related protein (ADRP) expression in adipocyte precursors by long‐chain fatty acids
J. Gao (2000)
10.1002/jcb.21044
Prostaglandin F2α inhibits adipocyte differentiation via a Gαq‐Calcium‐Calcineurin‐Dependent signaling pathway
L. Liu (2007)
10.1016/J.CELLSIG.2003.09.001
Prostaglandin F2alpha increases glucose transport in 3T3-L1 adipocytes through enhanced GLUT1 expression by a protein kinase C-dependent pathway.
Guang Yuh Chiou (2004)
10.1051/RND:2000103
The adipose conversion process: regulation by extracellular and intracellular factors.
C. Boone (2000)
10.1167/iovs.18-25134
The Effect of Prostaglandin Analogue Bimatoprost on Thyroid-Associated Orbitopathy
Catherine J Choi (2018)
10.1007/s00109-002-0384-9
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
10.1093/jb/mvn152
Prostaglandin F2alpha synthase activities of aldo-keto reductase 1B1, 1B3 and 1B7.
Z. Kabututu (2009)
10.1146/ANNUREV.NU.14.070194.000531
Regulation of adipocyte development.
P. Cornelius (1994)
10.1152/PHYSREV.1998.78.3.783
Understanding adipocyte differentiation.
F. Gregoire (1998)
10.5772/intechopen.88746
Mediators of Impaired Adipogenesis in Obesity-Associated Insulin Resistance and T2DM
Haya Al-Sulaiti (2019)
10.1002/jcb.24785
Prostaglandin F2α Inhibits Adipogenesis Via an Autocrine‐Mediated Interleukin‐11/Glycoprotein 130/STAT1‐Dependent Signaling Cascade
D. Annamalai (2014)
10.1074/jbc.M707775200
cAMP-dependent Signaling Regulates the Adipogenic Effect of n-6 Polyunsaturated Fatty Acids*
L. Madsen (2008)
10.1016/0005-2760(93)90196-G
Lipoprotein lipase gene expression: physiological regulators at the transcriptional and post-transcriptional level.
S. Enerbäck (1993)
10.1002/jcb.21801
Prostaglandin F2α induces the normoxic activation of the hypoxia‐inducible factor‐1 transcription factor in differentiating 3T3‐L1 preadipocytes: Potential role in the regulation of adipogenesis
L. Liu (2008)
Inhibition of adipogenesis by the c-myc oncoprotein.
Heath Vj (1997)
10.1161/ATVBAHA.112.300230
Macrophages Regulate Smooth Muscle Differentiation of Mesenchymal Stem Cells via a Prostaglandin F2&agr;−Mediated Paracrine Mechanism
M. Lee (2012)
10.3341/kjo.2014.28.3.257
Topical Prostaglandin Analogue Drugs Inhibit Adipocyte Differentiation
J. Kim (2014)
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