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Rhamnogalacturonase: A Novel Enzyme That Degrades The Hairy Regions Of Pectins

H. Schols, Carolien C.J.M. Geraeds, M. F. S. Leeuwen, F. Kormelink, A. G. Voragen
Published 1990 · Biology

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A rhamnogalacturonase (RGase), that could degrade the modified hairy regions (MHR) prepared from apple cell walls, was isolated and purified from a technical preparation of Aspergillus aculeatus. The RGase cleaved galactopyranosyluronic-rhamnopyranosyl linkages. No activity was observed towards other cell-wall polysaccharides or p-nitrophenyl glycosides. The optimal conditions for RGase were pH 3–4 and 40–50°. MHR was degraded by RGase, and methylation analysis and 13C-n.m.r. spectroscopy indicated the products to have a tetrasaccharide backbone of alternating rhamnose and galacturonic acid residues. Some oligomers had a galactose residue 4-linked to rhamnose. The potential value of this new enzyme is considered.



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