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Fasciola Hepatica: Molecular Cloning, Nucleotide Sequence, And Expression Of A Gene Encoding A Polypeptide Homologous To A Schistosoma Mansoni Fatty Acid-binding Protein.
J. Rodríguez-Pérez, J. Rodríguez-Medina, M. Garcia-Blanco, G. Hillyer
Published 1992 · Biology, Medicine
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Immunization of mice with an antigenic polypeptide from Fasciola hepatica adult worms and having an apparent molecular mass of 12,000 Da (Fh12) has been shown to reduce the worm burden from challenge infection with Schistosoma mansoni by more than 50%. Moreover, mice infected with S. mansoni develop antibodies to Fh12 after 5-6 weeks of infection, indicating that this Fasciola-derived antigen is a cross-reactive, cross-protective protein. A lambda gt11 F. hepatica cDNA library was constructed from poly(A)+ RNA extracted from adult worms. A cDNA encoding a cross-reactive polypeptide (Fh15) was cloned by screening the F. hepatica lambda gt11 library with a monospecific, polyclonal rabbit antiserum against pure, native Fh12. The cDNA was sequenced and the predicted amino acid sequence revealed an open reading frame encoding a 132-amino-acid protein with a predicted molecular weight of 14,700 Da. This protein has significant homology to a 14-kDa S. mansoni fatty acid-binding protein. Comparison of the protective-inducing activity of recombinant Fh15 with that of purified Fh12 against schistosomes and Fasciola is warranted.
This paper references
Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis.
V. C. Tsang (1983)
3′ Non-coding region sequences in eukaryotic messenger RNA
N. Proudfoot (1976)
Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.
D. Smith (1986)
A simple and very efficient method for generating cDNA libraries.
U. Gubler (1983)
DNA sequencing with chain-terminating inhibitors.
F. Sanger (1977)
A 14-kDa Schistosoma mansoni polypeptide is homologous to a gene family of fatty acid binding proteins.
D. Moser (1991)
Rapid similarity searches of nucleic acid and protein data banks.
W. Wilbur (1983)
Schistosomiasis and filariasis
G. Hillyer (1991)
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
M. Kozak (1987)
In the beginning is the end: regulation of poly(A) addition and removal during early development.
M. Wickens (1990)
Induction of immunity in mice to Fasciola hepatica with a Fasciola/Schistosoma cross-reactive defined immunity antigen.
G. Hillyer (1985)
Molecular cloning of a protective antigen of schistosomes
J. M. Balloul (1987)
Methods in Enzymology , Vol
S. Colowick (1966)
Identification of a 17-kilodalton Fasciola hepatica immunodiagnostic antigen by the enzyme-linked immunoelectrotransfer blot technique.
G. Hillyer (1988)
Acquired immunity in schistosomiasis with purified Fasciola hepatica cross-reactive antigens.
G. Hillyer (1988)
Efficient isolation of genes by using antibody probes.
R. Young (1983)
Cell-mediated immune response to schistosomiasis.
S. L. James (1990)
Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA.
C. A. Baxa (1989)
Acquired resistance to Fasciola hepatica in cattle using a purified adult worm antigen.
G. Hillyer (1987)
Successful vaccination against murine Schistosoma mansoni infection with a purified 12 Kd Fasciola hepatica cross-reactive antigen.
G. Hillyer (1988)
Protective immunity and vaccines to schistosomiasis.
D. Colley (1989)
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S. Mahanty (2011)
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O. Córdoba (1998)
Gene cloning, overproduction and purification of a functionally active cytoplasmic fatty acid-binding protein (Sj-FABPC) from the human blood fluke Schistosoma japonicum.
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