Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Relationship Between Excision Repair And The Cytotoxic And Mutagenic Effect Of The 'anti' 7,8-diol-9,10-epoxide Of Benzo[a]pyrene In Human Cells.

L. Yang, V. Maher, J. Mccormick
Published 1982 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
The cytotoxic and mutagenic effect of (+/-)-7 beta, 8 alpha-dihydroxy-9 alpha, 10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (anti BPDE) in normally excising diploid human cells treated just prior to onset of S was compared with that of cells allowed approximately 16 h for excision repair before onset of S and with that observed in excision-deficient xeroderma pigmentosum (XP12BE) cells. The cells were synchronized by release from density inhibition of cell replication. DNA synthesis began approximately 22 h after the cells were plated at lower density (i.e., 1.4 x 10(4) cells/cm2). The frequency of thioguanine-resistant mutants induced in normal cells treated just prior to onset of S was approximately 12- to 16-fold higher than that observed in cells treated in early G1 or treated in G0 (confluence) and then plated at lower density. The frequency approximated that expected for XP12BE cells from extrapolation of data obtained at lower doses. The frequency of mutants measured in normal cells treated in exponential growth was also much higher than that in the cells treated in early G1 or in G0. No such difference could be seen in XP12BE cells treated in exponential growth or in G0. In contrast to the mutagenicity data in the normal cells, there was no significant difference in the slope of the survival curve of normal cells treated at various times prior to S phase at low densities. However, normal cells treated even at the onset of S exhibited survival equal to XP12BE cells given a 4- to 5-fold lower dose. The data support the hypothesis that DNA synthesis is the cellular event which converts unexcised DNA lesions into mutations. However, they indicate that S is not the event primarily responsible for translating DNA damage into cell death. Accompanying studies on the rate of excision of anti BPDE adducts from the normal cells during the period prior to S support the conclusions.
This paper references
A comparison of the DNA binding, cytotoxicity and repair synthesis induced in human fibroblasts by reactive derivatives of aromatic amide carcinogens.
R. Heflich (1980)
Error-free excision of the cytotoxic,mutagenic N2-deoxyguanosine DNA adduct formed in human fibroblasts by (+/-)-7 beta, 8 alpha-dihydroxy-9 alpha, 10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene.
L. Yang (1980)
Recovery from inhibition by radiation of transcriptionally controlled enzyme induction
E. Ben-Hur (1980)
Effect of DNA repair on the cytotoxicity and mutagenicity of polycyclic hydrocarbon derivatives in normal and xeroderma pigmentosum human fibroblasts.
V. Maher (1977)
Typical xeroderma pigmentosum complementation group A fibroblasts have detectable ultraviolet light-induced unscheduled DNA synthesis.
R. Petinga (1977)
Excision of hydrocarbon-DNA adducts and consequent cell survival in normal and repair defective human cells.
B. Mccaw (1978)
Extent of excision repair before DNA synthesis determines the mutagenic but not the lethal effect of UV radiation.
B. Konze-Thomas (1982)
Xeroderma pigmentosum. An inherited diseases with sun sensitivity, multiple cutaneous neoplasms, and abnormal DNA repair.
J. H. Robbins (1974)
DNA excision-repair processes in human cells can eliminate the cytotoxic and mutagenic consequences of ultraviolet irradiation.
V. Maher (1979)
Correlation among the rates of dimer excision, DNA repair replication, and recovery of human cells from potentially lethal damage induced by ultraviolet radiation.
B. Konze-Thomas (1979)
Reactive derivatives of benzo(a)pyrene and 7,12-dimethylbenz(a)anthracene cause S1 nuclease sensitive sites in DNA and "UV-like" repair.
R. Heflich (1977)

This paper is referenced by
The effects of cell cycle position on the cytotoxicity and mutagenicity of benzo(a)pyrene in cultured Chinese hamster cells
Ochi Takafumi (1985)
Benzo(a)pyrene:DNA adduct formation in early-passage Wistar rat embryo cell cultures: evidence for multiple pathways of activation of benzo(a)pyrene.
D. Pruess-Schwartz (1986)
Detection of DNA effects in human cells with the comet assay and their relevance for mutagenesis.
G. Speit (1996)
Expression of common chromosomal fragile site genes, WWOX/FRA16D and FHIT/FRA3B is downregulated by exposure to environmental carcinogens, UV, and BPDE but not by IR
E. Thavathiru (2005)
Pathways of human cell post-replication repair.
W. Kaufmann (1989)
Evidence that error-prone DNA repair converts dibenzo[a,l]pyrene-induced depurinating lesions into mutations: formation, clonal proliferation and regression of initiated cells carrying H-ras oncogene mutations in early preneoplasia.
D. Chakravarti (2000)
Relationship between excision repair and the cytotoxic and mutagenic action of chemicals and UV radiation.
V. Maher (1983)
Persistenceof Benzo(a)pyrene Metabolite:DNA Adductsin Lungand Liver of Mice
Mahmooda S. Kulkarni (1984)
Cytotoxic and mutagenic effects of specific carcinogen-DNA adducts in diploid human fibroblasts.
J. Mccormick (1985)
Molecular cloning of a mammalian gene involved in the fixation of UV-induced mutations
S. Bouffler (1990)
Alkylation damage, DNA repair and mutagenesis in human cells.
V. Maher (1990)
The role of cell proliferation in chemically induced carcinogenesis.
J. Foster (1997)
Chromium can reduce the mutagenic effects of benzo[a]pyrene diolepoxide in normal human fibroblasts via an oxidative stress mechanism.
Y. Tesfai (1998)
Differential mutagenicity and cytotoxicity of (±)‐benzo[a]pyrene‐trans‐7,8‐dihydrodiol and (±)‐anti‐benzo[a]pyrene‐trans‐7,8‐dihydrodiol‐9, 10‐epoxide in genetically engineered human fibroblasts
T. Quan (1995)
Studies on Chemically Induced Neoplastic Transformation and Mutation in the BALB/3T3 Cl A31-1-1 Cell Line in Relation to the Quantitative Evaluation of Carcinogens
U. Saffiotti (1984)
From DNA damage to mutation in mammalian cells: A review
T. Rossman (1988)
Induction of Rad51 protein levels by p38 MAPK decreases cytotoxicity and mutagenicity in benzo[a]pyrene-exposed human lung cancer cells.
Show-Mei Chuang (2008)
Analysis of benzo(a)pyrene:DNA adducts formed in cells in culture by immobilized boronate chromatography.
D. Pruess-Schwartz (1984)
Repair of tobacco carcinogen-induced DNA adducts and lung cancer risk: a molecular epidemiologic study.
Q. Wei (2000)
Detection of in vivo DNA repair synthesis in mouse liver and lung induced by treatment with benzo(a)pyrene or 4-nitroquinoline 1-oxide.
M. Kulkarni (1984)
The HPRT Gene as a Model System for Mutation Analysis
V. Maher (1996)
DNA Polymerase Eta Participates in the Mutagenic Bypass of Adducts Induced by Benzo[a]pyrene Diol Epoxide in Mammalian Cells
A. Klarer (2012)
Role of DNA Damage and Repair in the Mutagenesis of Human Cells by Carcinogens
V. Maher (1985)
Repair analysis of promutagenic (+)-anti-BPDE DNA adduct in transcriptionally active sequences of plasmid DNA in Escherichia coli.
J. Musarrat (1997)
Role of DNA lesions and repair in the transformation of human cells.
V. Maher (1984)
Reactions of stereoisomeric and structurally related bay region diol epoxide derivatives of benz[a]anthracene with DNA. Conformations of noncovalent complexes and covalent carcinogen-DNA adducts.
S. Carberry (1988)
Anticarcinogenic potential of DNA-repair modulators.
D. Boothman (1988)
Processing of psoralen adducts in an active human gene: Repair and replication of DNA containing monoadducts and interstrand cross-links
J. Vos (1987)
Subsulfide Can Protect Human Fibroblasts from the Mutagenic Effect of Benzo [ A ] Pyrene
Diolepoxide (2019)
Genotoxicity of formaldehyde in cultured human bronchial fibroblasts.
R. Grafstrom (1985)
Use of PCR amplification of cDNA to study mechanisms of human cell mutagenesis and malignant transformation
V. Maher (1991)
Chapter 8 – Measurement of Mutations in Somatic Cells in Culture
Veronica M. Maher (1982)
See more
Semantic Scholar Logo Some data provided by SemanticScholar