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Stability And Kinetic Properties Of Humus-urease Complexes

P. Nannipieri, B. Ceccanti, S. Cervelli, P. Sequi
Published 1978 · Chemistry

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Abstract We have determined the kinetics and stabilities of various humus-urease preparations, extracted and purified from soil (Ceccanti, Nannipieri, Cervelli and Sequi, 1977). Km, values of five different fractions in phosphate buffer ranged from 8 to 40 mM and were similar to those of urease extracted from various other sources. However, the optimal pH for urease activity of the soil fractions ranged from 7.5 to 8.8, in contrast to a pH optimum of about 7.0 for urease from sources other than soil. The shift in the optimal pH towards the alkaline region may be due to the influence of the negative charge of the humus matrix localized around the enzyme. Urease organo-complexes of mol. wt. 105 were even more resistant, due probably to the occurrence of molecular arrangements similar to that proposed by Burns, El-Sayed and McLaren (1972a) and Burns, Pukite and McLaren (1972b). Thus although each of the five urease-active organic matter fractions exhibited different characteristics, they could be grouped into two distinct classes, according to mol. wt. and resistance to thermal denaturation and proteolysis.
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