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Promoter Selection In Human Mitochondria Involves Binding Of A Transcription Factor To Orientation-independent Upstream Regulatory Elements

R. P. Fisher, J. N. Topper, D. Clayton
Published 1987 · Biology, Medicine

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Selective transcription of human mitochondrial DNA requires a transcription factor (mtTF) in addition to an essentially nonselective RNA polymerase. Partially purified mtTF is able to sequester promoter-containing DNA in preinitiation complexes in the absence of mitochondrial RNA polymerase, suggesting a DNA-binding mechanism for factor activity. Functional domains, required for positive transcriptional regulation by mtTF, are identified within both major promoters of human mtDNA through transcription of mutant promoter templates in a reconstituted in vitro system. These domains are essentially coextensive with DNA sequences protected from nuclease digestion by mtTF-binding. Comparison of the sequences of the two mtTF-responsive elements reveals significant homology only when one sequence is inverted; the binding sites are in opposite orientations with respect to the predominant direction of transcription. Thus mtTF may function bidirectionally, requiring additional protein-DNA interactions to dictate transcriptional polarity. The mtTF-responsive elements are arrayed as direct repeats, separated by approximately 80 bp within the displacement-loop region of human mitochondrial DNA; this arrangement may reflect duplication of an ancestral bidirectional promoter, giving rise to separate, unidirectional promoters for each strand.
This paper references
Homologous interactions of h repressor and A Cro with the A operator
A. Hochschild (1986)
10.1073/PNAS.83.17.6277
In vitro transcription of human mitochondrial DNA: accurate termination requires a region of DNA sequence that can function bidirectionally.
T. Christianson (1986)
Identification and in vitro capping of a primary transcript of human mitochondrial DNA.
B. Yoza (1984)
10.1016/0092-8674(84)90061-8
Identification of a promoter for transcription of the heavy strand of human mtDNA: In vitro transcription and deletion mutagenesis
D. Bogenhagen (1984)
Identification of initiation sites for transcription of Xenopus laevis mitochondrial DNA.
D. Bogenhagen (1986)
10.1016/S0076-6879(80)65059-9
Sequencing end-labeled DNA with base-specific chemical cleavages.
A. Maxam (1980)
10.1126/SCIENCE.6283634
Transcriptional control signals of a eukaryotic protein-coding gene.
S. McKnight (1982)
10.1073/PNAS.82.2.351
Priming of human mitochondrial DNA replication occurs at the light-strand promoter.
D. D. Chang (1985)
10.1038/290457A0
Sequence and organization of the human mitochondrial genome
S. Anderson (1981)
10.1128/MCB.6.1.294
Minor transcription initiation events indicate that both human mitochondrial promoters function bidirectionally.
D. D. Chang (1986)
10.1002/j.1460-2075.1985.tb03817.x
Replication priming and transcription initiate from precisely the same site in mouse mitochondrial DNA.
D. D. Chang (1985)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
10.1016/0092-8674(86)90266-7
Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk gene
B. Graves (1986)
A transcription factor required for promoter recognition by human mitochondrial RNA polymerase. Accurate initiation at the heavy- and light-strand promoters dissected and reconstituted in vitro.
R. P. Fisher (1985)
New M13 vectors for cloning.
J. Messing (1983)
the initiation sites for transcription of Xenopus laevis mitochondrial DNA
D D. (1984)
10.1016/0092-8674(86)90523-4
How λ repressor and λ Cro distinguish between OR1 and OR3
A. Hochschild (1986)
10.1126/SCIENCE.6356356
Transcription of class III genes: formation of preinitiation complexes.
A. Lassar (1983)
10.1016/0092-8674(86)90559-3
Human rRNA transcription is modulated by the coordinate binding of two factors to an upstream control element
R. M. Learned (1986)
10.1073/PNAS.82.9.2660
Initiation of transcription from each of the two human mitochondrial promoters requires unique nucleotides at the transcriptional start sites.
J. Hixson (1985)
10.1126/SCIENCE.3018925
Tandem duplication of D-loop and ribosomal RNA sequences in lizard mitochondrial DNA.
C. Moritz (1986)
10.1128/MCB.6.9.3262
Precise assignment of the heavy-strand promoter of mouse mitochondrial DNA: cognate start sites are not required for transcriptional initiation.
D. D. Chang (1986)
10.1093/NAR/5.9.3157
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
D. Galas (1978)
Howl repressor and 1 Cro distinguish between OR 1 and OR 3
A. Hochschild (1986)
10.1016/0092-8674(86)90015-2
Homologous interactions of λ repressor and λ Cro with the λ operator
A. Hochschild (1986)
10.1146/ANNUREV.BI.53.070184.003041
Transcription of the mammalian mitochondrial genome.
D. Clayton (1984)
10.1093/NAR/9.20.5411
Sequence and properties of the human KB cell and mouse L cell D-loop regions of mitochondrial DNA.
M. W. Walberg (1981)
10.1093/NAR/12.18.7035
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
D. Melton (1984)
10.1016/0092-8674(84)90343-X
Precise identification of individual promoters for transcription of each strand of human mitochondrial DNA
D. D. Chang (1984)
In vitro transcription of human mitochondrial DNA. Identification of specific light strand transcripts from the displacement loop region.
M. W. Walberg (1983)
10.1016/0092-8674(85)90112-6
Lambda repressor mutations that increase the affinity and specificity of operator binding
H. C. Nelson (1985)
10.1128/MCB.6.9.3253
Precise assignment of the light-strand promoter of mouse mitochondrial DNA: a functional promoter consists of multiple upstream domains.
D. D. Chang (1986)
10.1016/0092-8674(83)90410-5
Enhancer elements
G. Khoury (1983)
10.1128/MCB.6.5.1446
Identification of primary transcriptional start sites of mouse mitochondrial DNA: accurate in vitro initiation of both heavy- and light-strand transcripts.
D. D. Chang (1986)
10.1016/0076-6879(83)01005-8
[2] New M13 vectors for cloning
J. Messing (1983)



This paper is referenced by
10.1101/GAD.7.12A.2431
NRF-1, an activator involved in nuclear-mitochondrial interactions, utilizes a new DNA-binding domain conserved in a family of developmental regulators.
C. A. Virbasius (1993)
10.1073/pnas.1119738109
Mammalian transcription factor A is a core component of the mitochondrial transcription machinery
Y. Shi (2012)
10.1074/jbc.M206958200
Mitochondrial Transcription Factor A and Its Downstream Targets Are Up-regulated in a Rat Hepatoma*
X. Dong (2002)
10.1006/BBRC.2001.5528
Drosophila mitochondrial transcription factor A: characterization of its cDNA and expression pattern during development.
K. Takata (2001)
10.1016/0168-9525(87)90291-5
The selfish organelle
H. T. Jacobs (1987)
10.1016/0014-5793(90)80841-6
Acetyl‐L‐carnitine increases cytochrome oxidase subunit I mRNA content in hypothyroid rat liver
M. Gadaleta (1990)
39 DNA Replication
D. A. Clayton (1996)
10.1016/J.GENE.2005.07.007
History of the Tfam gene in primates.
I. D'Errico (2005)
10.2353/ajpath.2008.071163
Expression and maintenance of mitochondrial DNA: new insights into human disease pathology.
G. Shadel (2008)
10.1038/nrg1708
The organization and inheritance of the mitochondrial genome
X. Chen (2005)
10.1016/0092-8674(87)90013-4
Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7
B. S. Masters (1987)
10.1006/BBRC.1997.6787
The mitochondrion as a primary site of action of glucocorticoids: mitochondrial nucleotide sequences, showing similarity to hormone response elements, confer dexamethasone inducibility to chimaeric genes transfected in LATK- cells.
C. Tsiriyotis (1997)
10.1128/MCB.15.12.7032
Distinct roles for two purified factors in transcription of Xenopus mitochondrial DNA.
I. Antoshechkin (1995)
10.1093/HUMREP/15.SUPPL_2.11
Transcription and replication of mitochondrial DNA.
D. Clayton (2000)
10.1091/MBC.E03-02-0099
Glom is a novel mitochondrial DNA packaging protein in Physarum polycephalum and causes intense chromatin condensation without suppressing DNA functions.
N. Sasaki (2003)
10.1007/978-3-7091-9138-5
Cell Organelles
R. Herrmann (1992)
10.1007/978-1-4757-9065-8
Advances in Human Genetics
D. Wallace (1990)
10.1016/0020-711X(89)90124-9
A proposal for a possible role of nucleosome positioning in the evolutionary adjustment of introns.
A. Csordás (1989)
10.1016/S0074-7696(08)62068-9
The endosymbiont hypothesis revisited.
M. Gray (1992)
10.1016/S1877-3419(09)70065-0
Chapter 6 Pathophysiology of Mitochondrial Disease as Illuminated by Animal Models
D. Wallace (2002)
10.1016/j.cell.2011.06.051
The Human Mitochondrial Transcriptome
T. Mercer (2011)
10.1007/s002940050446
DNA-binding factors assemble in a sequence-specific manner on the maize mitochondrial atpA promoter
Ching-Chun Chang (1999)
10.1093/nar/gkz762
Human mitochondrial DNA is extensively methylated in a non-CpG context
Vibha Patil (2019)
10.1104/PP.116.2.519
Characterization of DNA-Binding Proteins from Pea Mitochondria
Hatzack (1998)
10.1097/01.WNR.0000204980.98876.11
Activity-dependent regulation of nuclear respiratory factor-1, nuclear respiratory factor-2, and peroxisome proliferator-activated receptor gamma coactivator-1 in neurons
H. Liang (2006)
10.1371/journal.pgen.1000474
Ancient mtDNA Genetic Variants Modulate mtDNA Transcription and Replication
S. Suissa (2009)
10.1007/978-1-61779-328-8_21
Monitoring mitophagy in neuronal cell cultures.
Jianhui Zhu (2011)
10.1172/JCI118136
Thyroid hormone-regulated brain mitochondrial genes revealed by differential cDNA cloning.
E. Vega-Núñez (1995)
10.1007/978-3-662-12509-0_5
Mitochondrial DNA Replication
K. Keshav (1998)
10.1093/nar/gkp750
Biophysical characterizations of human mitochondrial transcription factor A and its binding to tumor suppressor p53
T. S. Wong (2009)
10.1093/nar/gks424
Mammalian NUMT insertion is non-random
J. Tsuji (2012)
10.17077/ETD.XC6H2QQA
Phosphoregulation of DRP1 at the mitochondria in vivo regulates ischemic sensitivity in the brain and memory
Kyle H Flippo (2017)
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