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Pectic Enzymes From Aureobasidium Pullulans LV 10

P. L. Manachini, C. Parini, M. G. Fortina
Published 1988 · Chemistry

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Abstract Aureobasidium pullulans LV 10 produced extracellular pectolytic activity when grown in a medium containing apple pectin as a carbon source. Maximum enzyme production (22 PGU ml −1 and 9 PLU ml −1 ) was obtained after 4 days of batch growth. Two pectin hydrolases and two pectin lyases by CM-Sepharose 6B, DEAE-cellulose, and Sephadex G-100 column chromatography were partially purified and characterized. Hydrolases I and II gave optimum activity at pH 5.5 and 4.5, respectively, at 50°C. For lyases I and II, the optimum occurred at pH 5.0 and 7.5, respectively, and 40°C.



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