Online citations, reference lists, and bibliographies.
← Back to Search

Regulation Of The Secretion Of Lipoprotein Lipase By Mouse Macrophages.

S. Behr, F. Kraemer
Published 1986 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The regulation of the secretion of lipoprotein lipase was studied in primary cultures of mouse peritoneal macrophages and in the murine macrophage cell line J774. As previously reported, both cell types secrete a lipase with the characteristics of lipoprotein lipase. Incubation of macrophages with insulin, insulin-like growth factor, and L-thyroxine had no effect on lipoprotein lipase secretion. Incubation with dexamethasone and with several agents which increase intracellular cyclic AMP led to a decrease in lipoprotein lipase secretion by mouse peritoneal macrophages. These results suggest that the hormonal regulation of lipoprotein lipase in macrophages is different from that in adipose tissue and heart muscle. Incubation of the macrophages with heparin caused a marked increase in the secretion of lipoprotein lipase. Short incubations with heparin (5 min) caused a release of the enzyme into the media, while longer incubations caused a 2-8-fold increase in net lipoprotein lipase secretion which was maximal after 2-16 h depending on cell type, and persisted for 24 h. The effect of heparin was dose-dependent and specific (it was not duplicated by other glycosaminoglycans). The mechanism of heparin-induced increase in lipoprotein lipase secretion was explored. The increase was not caused by the release of a presynthesized intracellular pool of lipoprotein lipase or by the stabilization of lipoprotein lipase by heparin after secretion. The heparin-induced increase in lipoprotein lipase secretion was dependent on protein synthesis. The secretion of lipoprotein lipase by macrophages in response to low levels of heparin may be a significant factor in the formation of atherosclerotic lesions.
This paper references
Insulin regulation of lipoprotein lipase activity and release in 3T3-L1 adipocytes. Separation and dependence of hormonal effects on hexose metabolism and synthesis of RNA and protein.
P. Spooner (1979)
10.1055/S-0028-1093768
Effect of Glucocorticoids on Lipoprotein Lipase Activity in Rat Heart and Adipose Tissue
P. D. Gasquet (1975)
10.1016/0005-2760(80)90063-6
Effects of metabolic inhibitors on the synthesis and release of lipoprotein lipase in cultured cells derived from the stromal-vascular fraction of rat adipose tissue.
J. Glick (1980)
Study of lipoprotein lipase content in Ob17 preadipocytes during adipose conversion. Immunofluorescent localization of the enzyme.
C. Vannier (1982)
10.1016/0006-291X(82)91337-7
Human monocytes in culture synthesize and secrete lipoprotein lipase.
P. Wang-Iverson (1982)
10.1016/0005-2760(78)90146-7
Lipoprotein lipase of cultured mesenchymal rat heart cells. III. Effect of glucocorticoids and insulin on enzyme formation.
G. Friedman (1978)
Triglyceride, diglyceride, monoglyceride, and cholesterol ester hydrolases in chicken adipose tissue activated by adenosine 3':5'-Monophosphate-dependent protein kinase. Chromatographic resolution and immunochemical differentiation from lipoprotein lipase.
J. C. Khoo (1976)
The reciprocal regulation of lipoprotein lipase activity and hormone-sensitive lipase activity in rat adipocytes.
R. Patten (1970)
10.1042/BJ1680483
Binding, interiorization and degradation of cholesteryl ester-labelled chylomicron-remmant particles by rat hepatocyte monolayers.
C. H. Florén (1977)
10.1016/0005-2760(81)90219-8
Uptake of chylomicron remnants causes cholesterol accumulation in cultured human arterial smooth muscle cells.
C. H. Florén (1981)
10.1152/AJPLEGACY.1972.223.6.1271
Effects of insulin on lipoprotein lipase activity in the rat heart and adipose tissue.
J. Borensztajn (1972)
Assembly of the sarcoplasmic reticulum. Cell-free synthesis of te Ca2+ + Mg2+-adenosine triphosphatase and calsequestrin.
R. Reithmeier (1980)
Receptor-dependent uptake of human chylomicron remnants by cultured skin fibroblasts.
C. H. Florén (1981)
10.1016/0005-2760(81)90265-4
L-Lactate as a source of carbon for fatty acid synthesis in adult and foetal sheep.
J. Robertson (1981)
The role of lipoprotein lipase in the metabolism of triglyceride-rich lipoproteins by macrophages.
P. Lindqvist (1983)
10.1172/JCI110590
Hypertriglyceridemic very low density lipoproteins induce triglyceride synthesis and accumulation in mouse peritoneal macrophages.
S. Gianturco (1982)
Maturation and secretion of lipoprotein lipase in cultured adipose cells. I. Intracellular activation of the enzyme.
C. Vannier (1985)
10.1002/JCB.1982.240180404
Inhibition by Glucocorticoids of Endocytosis in a Macrophage‐Like Cell Line
S. Miller (1982)
10.1161/01.RES.33.6.633
A Proposal Linking Atherogenesis to the Interaction of Endothelial Lipoprotein Lipase with Triglyceride‐Rich Lipoproteins
D. B. Zilversmit (1973)
The role of the monocyte in atherogenesis: I. Transition of blood-borne monocytes into foam cells in fatty lesions.
Gerrity Rg (1981)
Neutral cholesterol esterase activity in macrophages and its enhancement by cAMP-dependent protein kinase.
J. C. Khoo (1981)
10.1016/0167-4889(83)90117-9
Lipoprotein lipase activity in cultured macrophage cell line J7742 and its increase in variants deficient in adenylate cyclase and cyclic AMP-dependent protein kinase
R. Melmed (1983)
Secretion of lipoprotein lipase by macrophages in culture.
J. C. Khoo (1981)
10.2337/diab.29.8.643
Dissociation Between Plasma Triglyceride Concentration and Tissue Lipoprotein Lipase Deficiency in Insulin-deficient Rats
Y. D. Chen (1980)
Influence of fatty acyl substitution on the composition and function of macrophage membranes.
E. Mahoney (1980)
Characterization of the binding site on thioglycolate-stimulated mouse peritoneal macrophages that mediates the uptake of very low density lipoproteins.
F. Kraemer (1983)



This paper is referenced by
10.1161/01.ATV.15.3.392
Lipoprotein lipase synergizes with interferon gamma to induce macrophage nitric oxide synthetase mRNA expression and nitric oxide production.
G. Renier (1995)
10.1016/0167-4889(92)90207-R
Regulation of lipoprotein lipase by dibutyryl cAMP, cholera toxin, Hepes and heparin in F1 heart-cell cultures.
G. Friedman (1992)
10.1007/s00109-002-0384-9
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
10.1002/jlb.47.1.79
Control of Lipoprotein Lipase Secretion by Macrophages: Effect of Macrophage Differentiation Agents
R. Goldman (1990)
10.1016/j.bbalip.2014.03.013
Physiological regulation of lipoprotein lipase.
S. Kersten (2014)
10.1161/01.ATV.11.5.1315
Effects of human recombinant macrophage colony-stimulating factor on the secretion of lipoprotein lipase from macrophages.
N. Mori (1991)
10.2337/DIABETES.50.3.660
Direct regulatory effect of fatty acids on macrophage lipoprotein lipase: potential role of PPARs.
S. E. Michaud (2001)
10.2337/diab.41.2.S77
Role of Lipoprotein Lipase and Apolipoprotein E Secretion by Macrophages in Modulating Lipoprotein Uptake: Possible Role in Acceleration of Atherosclerosis in Diabetes
F. Kraemer (1992)
10.1161/01.ATV.20.10.2212
Proliferative Effect of Lipoprotein Lipase on Human Vascular Smooth Muscle Cells
J. Mamputu (2000)
10.1016/0005-2760(89)90137-9
Control of lipoprotein lipase secretion in mouse macrophages.
R. Goldman (1989)
10.1002/jlb.48.2.193
Dependence on Ca2+ of Lipoprotein Lipase Stability
R. Goldman (1990)
10.1016/S0014-5793(02)02811-9
PPARγ ligands, troglitazone and pioglitazone, up‐regulate expression of HMG‐CoA synthase and HMG‐CoA reductase gene in THP‐1 macrophages
K. Iida (2002)
10.1161/01.ATV.12.12.1458
Regulation of lipoprotein lipase secretion in murine macrophages during foam cell formation in vitro. Effect of triglyceride-rich lipoproteins.
O. Sofer (1992)
10.1161/01.ATV.15.4.522
Effects of platelet-derived growth factor on the synthesis of lipoprotein lipase in human monocyte-derived macrophages.
T. Inaba (1995)
10.1210/JC.2003-030911
Enhanced lipoprotein lipase secretion and foam cell formation by macrophages of patients with growth hormone deficiency: possible contribution to increased risk of atherogenesis?
O. Serri (2004)
10.1016/S0005-2728(88)80087-2
Regulation of macrophage lipoprotein lipase secretion by the scavenger receptor.
Y. Murata (1988)
10.1128/IAI.63.3.858-864.1995
Lipopolysaccharide regulation of lipoprotein lipase expression in murine macrophages.
M. Hill (1995)
10.2337/diab.37.8.1076
Insulin Deficiency Decreases Lipoprotein Lipase Secretion by Murine Macrophages
S. Behr (1988)
Effects of cytokines on the production of lipoprotein lipase in cultured human macrophages.
U. Querfeld (1990)
10.1074/jbc.M010707200
Glucose and Insulin Stimulate Heparin-releasable Lipoprotein Lipase Activity in Mouse Islets and INS-1 Cells
W. S. Cruz (2001)
Semantic Scholar Logo Some data provided by SemanticScholar