Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Relationship Between TGF Alpha-induced DNA Synthesis And Prostaglandin Synthesis In Human HaCaT Keratinocytes.

B. Hanke, G. Fürstenberger, F. Marks
Published 1996 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The relationship between transforming growth factor alpha (TGF alpha)-induced cell proliferation and prostaglandin synthesis was investigated using growth-arrested human keratinocytes of the HaCaT line. Depending on the TGF alpha concentration, the stimulation of DNA synthesis (5-fold) was found to be either insensitive (at < 10 ng/ml TGF alpha) or sensitive (at > or = 20 ng/ml TGF alpha) to inhibition by both indomethacin, an inhibitor of prostaglandin synthases (PGHS) 1 and 2 and the PGHS 2-specific inhibitor NS-398. Indomethacin-effected inhibition did not correlate with cytotoxicity and was restricted to a narrow time window after growth factor administration. The indomethacin- and NS-398-sensitive mitogenic effect of TGF alpha correlated with an early increase of arachidonic acid release and prostaglandin (PGE2, PGF2alpha) synthesis, whereas the PGHS inhibitor-insensitive TGF alpha effect did not. TGF alpha-induced prostaglandin synthesis was due to a growth factor-induced PGHS-2 activity as indicated by its suppression by NS-398. However, attempts to overcome the PGHS inhibitor-dependent suppression of TGF alpha-induced DNA synthesis by adding prostaglandins (E1, E2, F2alpha, G2) to the cultures proved to be unsuccessful. Thus, TGF alpha-induced synthesis of prostaglandins seems not to be involved in the mediation of the mitogenic effect of the growth factor on human keratinocytes in culture.
This paper references
10.1016/S0014-4827(64)81025-9
MITOTIC CONTROL BY INTERNAL SECRETION: THE ROLE OF THE CHALONE-ADRENALIN COMPLEX.
W. Bullough (1964)
Differential proliferative response to linoleate in cultures of epithelial cells from normal human breast and fibroadenomas.
A. Balakrishnan (1989)
10.1016/0006-291X(80)90767-6
Early prostaglandin E synthesis is an obligatory event in the induction of cell proliferation in mouse epidermis in vivo by the phorbol ester TPA.
G. Fürstenberger (1980)
10.1016/0952-3278(92)90098-4
Regulation of proliferation of rat mammary tumor cells by inhibitors of cyclooxygenase and lipoxygenase.
P. P. Lee (1992)
10.1016/0076-6879(67)12127-7
[105] Determination of DNA concentration with diphenylamine
K. Burton (1968)
10.1126/SCIENCE.2916128
Overexpression of transforming growth factor alpha in psoriatic epidermis.
James T. Elder (1989)
10.1083/JCB.106.3.761
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line
P. Boukamp (1988)
10.1016/0092-8674(87)90179-6
Cell migration is essential for sustained growth of keratinocyte colonies: The roles of transforming growth factor-α and epidermal growth factor
Y. Barrandon (1987)
10.1021/AC00284A003
Enzyme immunoassays of eicosanoids using acetylcholine esterase as label: an alternative to radioimmunoassay.
P. Pradelles (1985)
10.1016/0090-6980(94)90074-4
NS-398, a new anti-inflammatory agent, selectively inhibits prostaglandin G/H synthase/cyclooxygenase (COX-2) activity in vitro.
N. Futaki (1994)
10.1016/s0021-9258(19)39645-0
Mitogenic signaling by epidermal growth factor (EGF), but not platelet-derived growth factor, requires arachidonic acid metabolism in BALB/c 3T3 cells. Modulation of EGF-dependent c-myc expression by prostaglandins.
J. A. Handler (1990)
10.1038/328817A0
Production and auto-induction of transforming growth factor-α in human keratinocytes
R. Coffey (1987)
10.1201/9781351072311
Growth Regulation and Carcinogenesis
W. Paukovits (1990)
10.1016/0968-0004(92)90007-V
Crosstalk among multiple signal-activated phospholipases.
M. Liscovitch (1992)
10.1172/JCI112283
Modulation of keratinocyte proliferation in vitro by endogenous prostaglandin synthesis.
A. Pentland (1986)
10.1126/SCIENCE.1902323
Regulation of Ras-GAP and the neurofibromatosis-1 gene product by eicosanoids.
J. Han (1991)
10.1007/978-1-4684-7902-7
Skin Pharmacology and Toxicology
Corrado L. Galli (1990)
10.1016/0006-291X(91)91026-9
Prostaglandins E2 and F2a mediate the increase in c-myc expression induced by EGF in primary rat hepatocyte cultures.
G. Skouteris (1991)
10.1016/0022-1759(83)90303-4
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
10.1016/0304-3835(81)90107-5
Arachidonic acid and prostaglandin E2 release and enhanced cell proliferation induced by the phorbol ester TPA in a murine epidermal cell line.
G. Fürstenberger (1981)
Antiproliferative effects of lipoxygenase inhibitors on malignant human hematopoietic cell lines.
D. Snyder (1989)
10.1007/978-1-4613-2605-2
Arachidonic Acid Metabolism and Tumor Promotion
Lawrence J. Marnett (1985)
10.1084/JEM.167.2.670
Detection of transforming growth factor alpha in normal, malignant, and hyperproliferative human keratinocytes
A. Gottlieb (1988)
10.1016/0014-5793(92)80122-W
Prostaglandin F2α decreases the affinity of epidermal growth factor receptors in Swiss mouse 3T3 cells via protein kinase C activation
Luis Jimenez de Asua (1992)
Arachidonate metabolism in macrophages is affected by albumin.
P. Dieter (1990)
10.1016/s0021-9258(18)54818-3
Prostaglandin F2 alpha stimulates proliferation of clonal osteoblastic MC3T3-E1 cells by up-regulation of insulin-like growth factor I receptors.
Y. Hakeda (1991)
Activation of cytosolic phospholipase A2 by transforming growth factor-alpha in HEL-30 keratinocytes.
R. Kast (1993)
10.1093/CARCIN/7.10.1745
Isolation, characterization and in vitro cultivation of subfractions of neonatal mouse keratinocytes: effects of phorbol esters.
G. Fürstenberger (1986)
10.1016/s0021-9258(17)38909-3
Stoichiometry and kinetics of the interaction of prostaglandin H synthase with anti-inflammatory agents.
R. Kulmacz (1985)
10.1016/0006-2952(76)90216-1
The importance of phospholipase-A2 in prostaglandin biosynthesis.
R. Flower (1976)
Effects of phorbol esters on basal epidermal cells derived from ear skin of adult guniea pigs.
C. Delescluse (1982)
Role of arachidonic acid metabolism in the mitogenic response of BALB/c 3T3 fibroblasts to epidermal growth factor.
R. Nolan (1988)
10.1016/0968-0004(93)90175-M
The effector interactions of p21ras.
M. Marshall (1993)
10.1038/265421a0
Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes
J. Rheinwald (1977)
10.1002/JCP.1041510214
Regulation of TGF‐α expression in human keratinocytes: PKC‐dependent and ‐independent pathways
S. B. Klein (1992)
10.1016/0006-291X(78)91697-2
Indomethacin inhibition of cell proliferation induced by the phorbolester TPA is reversed by prostaglandin E2 in mouse epidermis in vivo.
G. Fürstenberger (1978)



This paper is referenced by
Inhibition of cyclooxygenase-2 decreases DNA synthesis induced by platelet-derived growth factor in Swiss 3T3 fibroblasts.
E. Castaño (2000)
10.1090/S0002-9947-03-03285-9
A generalization of tight closure and multiplier ideals
N. Hara (2002)
10.1090/S0025-5718-05-01730-8
Computing the tight closure in dimension two
Holger Brenner (2005)
10.1090/S0002-9947-03-03391-9
SLOPES OF VECTOR BUNDLES ON PROJECTIVE CURVES AND APPLICATIONS TO TIGHT CLOSURE PROBLEMS
Holger Brenner (2003)
10.1090/S0894-0347-05-00514-X
ON THE ARITHMETIC OF TIGHT CLOSURE
Holger Brenner (2004)
10.1090/S0002-9939-04-07469-6
On rings with small Hilbert-Kunz multiplicity
Manuel Blickle (2003)
10.1090/S0002-9939-2011-10858-X
A formula for the *-core of an ideal
Louiza Fouli (2009)
10.1090/S0002-9939-2011-11074-8
F-blowups of F-regular surface singularities
N. Hara (2012)
10.1090/TRAN/7030
Uniform Bounds in F-Finite Rings and Lower Semi-Continuity of the F-Signature
Thomas Polstra (2015)
10.1090/S0002-9947-10-05166-4
An excellent $F$-pure ring of prime characteristic has a big tight closure test element
R. Sharp (2009)
10.1090/S0002-9939-04-07491-X
The vanishing of ₁^{}(⁺,) implies that is regular
Ian M. Aberbach (2004)
10.1090/S0002-9939-00-05626-4
Tight closure commutes with localization in binomial rings
Karen E. Smith (2000)
10.1090/S0002-9939-05-08223-7
Phantom depth and flat base change
Neil M. Epstein (2005)
10.1165/RCMB.2004-0071SU
Functional Genomics of Lung Disease
J. A. Whitsett (2004)
10.1090/PROC/12928
Some computations of generalized Hilbert-Kunz function and multiplicity
Hailong Dao (2015)
10.1111/J.0022-202X.2005.23922.X
Sebaceous glands and the prostaglandin pathway--key stones of an exciting mosaic.
C. Zouboulis (2005)
10.1090/S0002-9939-05-07843-3
There is no Bogomolov type restriction theorem for strong semistability in positive characteristic
Holger Brenner (2004)
10.1090/S0002-9939-2012-11398-X
The equations of Rees algebras of equimultiple ideals of deviation one
Ferran Muiños (2012)
10.1090/S1056-3911-2013-00610-4
ON THE BEHAVIOR OF TEST IDEALS UNDER FINITE MORPHISMS
Karl Schwede (2013)
10.1090/S1056-3911-04-00395-9
Log-terminal singularities and vanishing theorems via non-standard tight closure
H. Schoutens (2005)
10.1090/S0002-9939-02-06556-5
A non-standard proof of the Briançon-Skoda theorem
H. Schoutens (2002)
10.1090/PROC/12676
A sufficient condition for strong $F$-regularity
A. Stefani (2014)
10.1090/S0002-9947-07-04118-9
Phantom depth and stable phantom exactness
Neil M. Epstein (2005)
10.1090/TRAN/7176
On the existence of $F$-thresholds and related limits
A. Stefani (2016)
10.1090/S0002-9947-01-02695-2
Geometric interpretation of tight closure and test ideals
N. Hara (2001)
10.2140/ant.2018.12.61
F-signature and Hilbert–Kunz multiplicity : a combined approach and comparison
Thomas Polstra (2018)
10.1090/S0002-9947-99-02396-X
Tight closure, plus closure and Frobenius closure in cubical cones
M. McDermott (1999)
10.1090/S0002-9947-07-04134-7
Pure subrings of regular rings are pseudo-rational
H. Schoutens (2005)
10.1090/S0002-9947-07-04247-X
Graded annihilators of modules over the Frobenius skew polynomial ring, and tight closure
R. Sharp (2006)
10.1090/S0002-9939-10-10379-7
On a result of Faltings via tight closure
Tirdad Sharif (2010)
10.1090/SUGA/427
$F$-singularities: applications of characteristic $p$ methods to singularity theory
S. Takagi (2014)
10.1090/S0002-9947-04-03420-8
Ideals in a perfect closure, linear growth of primary decompositions, and tight closure
R. Sharp (2003)
See more
Semantic Scholar Logo Some data provided by SemanticScholar