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Apoptosis In A Unicellular Eukaryote (Trypanosoma Cruzi): Implications For The Evolutionary Origin And Role Of Programmed Cell Death In The Control Of Cell Proliferation, Differentiation And Survival.

J. C. Ameisen, T. Idziorek, O. Billaut-Mulot, M. Loyens, J. Tissier, A. Potentier, A. Ouaissi
Published 1995 · Biology, Medicine

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The origin of programmed cell death (PCD) has been linked to the emergence of multicellular organisms. Trypanosoma cruzi, a member of one of the earliest diverging eukaryotes, is a protozoan unicellular parasite that undergoes three major differentiation changes and requires two different hosts. We report that the in vitro differentiation of the proliferating epimastigote stage into the G0/G1 arrested trypomastigote stage is associated with massive epimastigote death that shows the cytoplasmic and nuclear morphological features and DNA fragmentation pattern of apoptosis, the most frequent phenotype of PCD in multicellular organisms. Apoptosis could be accelerated or prevented by modifying culture conditions or cell density, indicating that extracellular signals influenced the epimastigote decision between life and death. Epimastigotes responded to complement-mediated immunological agression by undergoing apoptosis, while undergoing necrosis in response to nonphysiological saponin-mediated damage. PCD may participate into the optimal adaptation of T. cruzi to its different hosts, and the avoidance of a local competition between a G0/G1 arrested stage and its proliferating progenitor. The existence of a regulated cell death programme inducing an apoptotic phenotype in a unicellular eukaryote provides a paradigm for a widespread role for PCD in the control of cell survival, which extends beyond the evolutionary constraints that may be specific to multicellular organisms and raises the question of the origin and nature of the genes involved. Another implication is that PCD induction could represent a target for therapeutic strategies against unicellular pathogens.



This paper is referenced by
10.1017/S0953756297005893
The role of morphogenetic cell death in the histogenesis of the mycelial cord of Agaricus bisporus and in the development of macrofungi
Mustapha Umar (1998)
10.1038/nrmicro2929
Cell death in parasitic protozoa: regulated or incidental?
William R. Proto (2013)
10.1017/S0031182001008332
Programmed cell death in a human intestinal parasite, Blastocystis hominis.
A. Nasirudeen (2001)
10.1091/MBC.12.10.3016
On the evolutionary conservation of the cell death pathway: mitochondrial release of an apoptosis-inducing factor during Dictyostelium discoideum cell death.
D. Arnoult (2001)
10.1074/JBC.M103950200
Bisphosphonates Are Potent Inhibitors of Trypanosoma cruzi Farnesyl Pyrophosphate Synthase*
A. Montalvetti (2001)
10.1016/J.TRSTMH.2006.03.010
Mechanisms of action of lysophospholipid analogues against trypanosomatid parasites.
J. Urbina (2006)
10.1590/S0074-02762009000900033
The importance of apoptosis for immune regulation in Chagas disease.
George A DosReis (2009)
10.1016/j.pt.2010.06.008
Programmed cell death in unicellular parasites: a prerequisite for sustained infection?
G. van Zandbergen (2010)
10.1371/journal.ppat.1002320
The Meaning of Death: Evolution and Ecology of Apoptosis in Protozoan Parasites
S. E. Reece (2011)
10.4236/OJAPO.2014.32004
Role of Apoptosis in Microbial Infection
Faris Q B Alenzi (2014)
10.1016/S0020-7519(02)00087-5
Apoptosis in the malaria protozoan, Plasmodium berghei: a possible mechanism for limiting intensity of infection in the mosquito.
E. Al-Olayan (2002)
10.1038/nrm1224
Cell-death alternative model organisms: why and which?
P. Golstein (2003)
10.1016/J.MOLBIOPARA.2005.09.001
Metacaspases of Trypanosoma cruzi: possible candidates for programmed cell death mediators.
G. Kosec (2006)
10.1007/978-0-387-76717-8_3
Programmed Cell Death and Trypanosomatids: A Brief Review
M. D. Soeiro (2008)
10.1016/j.cbpb.2010.01.010
Signaling mechanisms of apoptosis-like programmed cell death in unicellular eukaryotes.
I. V. Shemarova (2010)
10.1074/jbc.272.7.3961
Atovaquone, a Broad Spectrum Antiparasitic Drug, Collapses Mitochondrial Membrane Potential in a Malarial Parasite*
I. Srivastava (1997)
10.4267/10608/883
Contrôle mitochondrial de l'apoptose : la mort cellulaire programmée est-elle apparue à la suite de l'événement endosymbiotique à l'origine des mitochondries ?
B. Mignotte (1998)
10.1006/EXPR.1998.4249
Trypanosoma brucei: the dynamics of calcium movement between the cytosol, nucleus, and mitochondrion of intact cells.
Z. H. Xiong (1998)
10.1016/J.IJPARA.2004.10.002
The role of programmed cell death in Plasmodium-mosquito interactions.
H. Hurd (2004)
10.1186/1756-3305-4-44
Evolution of apoptosis-like programmed cell death in unicellular protozoan parasites
S. Kaczanowski (2010)
10.3390/molecules13102462
Alkaloids Induce Programmed Cell Death in Bloodstream Forms of Trypanosomes (Trypanosoma b. brucei)
Vera Rosenkranz (2008)
10.1111/nph.12649
Dehydroascorbate: a possible surveillance molecule of oxidative stress and programmed cell death in the green alga Chlamydomonas reinhardtii.
Omer Murik (2014)
10.1006/EXCR.2002.5496
A form of cell death with some features resembling apoptosis in the amitochondrial unicellular organism Trichomonas vaginalis.
O. Chose (2002)
10.1016/S0020-7519(02)00005-X
Recent advances in Blastocystis hominis research: hot spots in terra incognita.
K. S. Tan (2002)
10.1007/s000180300049
Ceramide mediates growth inhibition of the Plasmodium falciparum parasite
I. Pankova-Kholmyansky (2003)
10.1016/j.bbamcr.2008.01.018
Programmed cell death in protists.
M. Deponte (2008)
10.1038/s41598-017-00186-9
Methionine aminopeptidase 2 is a key regulator of apoptotic like cell death in Leishmania donovani
R. Kumar (2017)
10.1093/OXFORDJOURNALS.BMB.A011624
The bcl-2 family of proteins
R. Brown (1997)
10.1016/0378-1119(96)00254-5
Trypanosoma cruzi elongation factor 1-α: nuclear localization in parasites undergoing apoptosis
O. Billaut-Mulot (1996)
10.3389/fcimb.2012.00095
Programmed cell death in Leishmania: biochemical evidence and role in parasite infectivity
S. Gannavaram (2012)
10.1007/978-3-642-60441-6
Fingerprinting Methods Based on Arbitrarily Primed PCR
M. Micheli (1997)
10.1016/J.ACTATROPICA.2005.03.004
Stage-specific antileishmanial activity of an inhibitor of SIR2 histone deacetylase.
B. Vergnes (2005)
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