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Comparative Study Of Lipoprotein Lipase In White And Brown Fat Of Rat (Rattus Norvegicus)

D. Guerrier, H. Pellet
Published 1980 · Chemistry

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Abstract 1. 1. More lipoprotein lipase of acetone ether powders of rat brown and epididymal fat is extracted by ammonium buffer than by NaCl barbital buffer. The presence of NaCl in the buffer determines great instability of the enzymatic preparation. 2. 2. The lipoprotein lipase of these two tissues have the same chromatographic behaviour on heparin-sepharose. Total purification is about 900 times. 3. 3. Purified enzymes are unstable. We have been able to show that NaCl is to a great extent responsible for this instability. 4. 4. Minimum molecular size is 64,000 for white fat lipoprotein lipase and 67,000 for brown fat lipoprotein lipase. 5. 5. The characteristics of the purified enzymes are identical and are comparable with those generally described.
This paper references
Lipoprotein lipase from bovine milk. Isolation procedure, chemical characterization, and molecular weight analysis.
P. Iverius (1976)
10.1042/BJ1720239
Effect of nutrition on subcellular localization of rat fat-cell lipoprotein lipase.
A. Vanhove (1978)
10.1055/S-0028-1093708
The lipoprotein lipase activity of brown adipose tissue during early post-natal development of the normal and hypothyroid rat.
P. Hémon (1975)
The purification of a lipoprotein lipase from bovine skim milk.
T. Egelrud (1972)
10.1016/0006-291X(71)90645-0
Evidence for an ionic binding of lipoprotein lipase to heparin.
T. Olivecrona (1971)
10.1152/AJPLEGACY.1971.220.6.1852
Response of lipoprotein lipase in various tissues to cold exposure.
M. Radomski (1971)
10.1016/S0003-2697(76)80064-4
Assay of proteins in the presence of interfering materials.
A. Bensadoun (1976)
10.1016/0006-291X(74)90618-4
The metabolism of very low density lipoprotein and chylomicrons by purified lipoprotein lipase from rat heart and adipose tissue.
P. Dolphin (1974)
Isolation, molecular properties, and kinetic characterization of lipoprotein lipase from rat heart.
J. Chung (1977)
10.1172/JCI103259
A relation between non-esterified fatty acids in plasma and the metabolism of glucose.
V. Dole (1956)
Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma.
J. Boberg (1977)
10.1016/0005-2744(69)90219-8
Purification of lipoprotein lipase from rat post-heparin plasma.
C. Fielding (1969)
COLORIMETRIC ULTRAMICRO METHOD FOR THE DETERMINATION OF FREE FATTY ACIDS.
M. Novak (1965)
10.1016/0021-9150(75)90025-8
Rat heart lipoprotein lipase.
J. Twu (1975)
10.1016/0014-5793(73)80571-X
Purification of rat adipose tissue lipoprotein lipase.
H. Greten (1973)
10.1042/BJ1740447
Developmental changes in the activity of lipoprotein lipase (clearing-factor lipase) in rat lung, cardiac muscle, skeletal muscle and brown adipose tissue.
A. Cryer (1978)
10.1042/BJ1240677
Coupling of glycosaminoglycans to agarose beads (sepharose 4B).
P. Iverius (1971)
Purification of synthetic ribonuclease S-peptide derivatives by specific complex formation on columns of ribonuclease S-protein bound to agarose.
I. Kato (1969)
10.1016/0005-2760(74)90151-9
Studies on the assay of clearing factor lipase (lipoprotein lipase)
S. Riley (1974)
Purification and characterization of lipoprotein lipase from pig adipose tissue.
A. Bensadoun (1974)
10.1016/0005-2744(76)90042-5
Purification of rat adipose tissue lipoprotein lipase by affinity chromatography.
J. Etienne (1976)



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