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Effects Of Endothelin On Phospholipases And Generation Of Second Messengers In Cat Iris Sphincter And SV-CISM-2 Cells.

A. A. Abdel-Latif, K. H. Ding, R. Akhtar, S. Y. Yousufzai
Published 1996 · Biology, Medicine

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In both immortalized cat iris sphincter smooth muscle cells (SV-CISM-2 cells) and cat iris sphincter, endothelin-1 (ET-1) markedly increased the activities of phospholipase A2 (PLA2), as measured by the release of arachidonic acid (AA), phospholipase C (PLC), as measured by the production of inositol trisphosphate (IP3), and phospholipase D (PLD), as measured by the formation of phosphatidylethanol (PEt). In SV-CISM-2 cells, ET-1 induced AA release, IP3 production and PEt formation in a dose- and time-dependent manner. The dose-response studies showed that the peptide is more potent in activating PLD (EC50 = 1.2 nM) than in activating PLC (EC50 = 1.5 nM) or PLA2 (EC50 = 1.7 nM). The time course studies revealed that ET-1 activated the phospholipases in a temporal sequence in which PLA2 was stimulated first (t1/2 = 12 s), followed by PLC (t1/2 = 48 s) and lastly PLD (t1/2 = 106 s). In SV-CISM-2 cells, in contrast to the intact iris sphincter, sarafotoxin-c, an ETB receptor agonist, had no effect on the phospholipases, and indomethacin, a cyclooxygenase inhibitor, had no effect on the stimulatory effect of ET-1 on the phospholipases. These results suggest that in this smooth muscle cell line, ET-1 interacts with the ETA receptor subtype to activate, via G proteins, phospholipases A2, C and D in a temporal sequence.
This paper references
Molecular Identification of Guanine‐Nucleotide‐Binding Regulatory Proteins which Couple to Endothelin Receptors
M. Takigawa (1995)
Endothelins: molecular biology, biochemistry, pharmacology, physiology, and pathophysiology.
G. Rubanyi (1994)
A rapid method of total lipid extraction and purification.
E. Bligh (1959)
Species differences in the effects of endothelin-1 on myo-inositol trisphosphate accumulation, cyclic AMP formation and contraction of isolated iris sphincter of rabbit and other species.
A. A. Abdel-latif (1991)
Characterization of iris sphincter smooth muscle endothelin receptor subtypes which are coupled to cyclic AMP formation and polyphosphoinositide hydrolysis.
A. El-Mowafy (1994)
Endothelin-1 stimulates the release of arachidonic acid and prostaglandins in rabbit iris sphincter smooth muscle: activation of phospholipase A2.
A. A. Abdel-Latif (1991)
Endothelin-1 stimulates phosphatidylinositol hydrolysis in the iris/ciliary complex and is a potent constrictor of the sphincter muscle.
N. Osborne (1992)
Immortalization of cat iris sphincter smooth muscle cells by SV40 virus: growth, morphological, biochemical and pharmacological characteristics.
A. Ocklind (1995)
Crosstalk among multiple signal-activated phospholipases.
M. Liscovitch (1992)
Sarafotoxin S6c: an agonist which distinguishes between endothelin receptor subtypes.
D. Williams (1991)
Activation of phospholipase D by endothelin-1 and other pharmacological agents in rabbit iris sphincter smooth muscle.
Y. Zhang (1992)
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)

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