Online citations, reference lists, and bibliographies.
← Back to Search

Cytokines Induce Catabolic Effects In Cultured Adipocytes By Multiple Mechanisms.

W. Doerrler, K. Feingold, C. Grunfeld
Published 1994 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Previous studies have demonstrated that some cytokines induce a coordinate catabolic response in adipose cells which leads to decreased fat storage. The mechanisms by which cytokines cause these effects are unknown. The primary purpose of the present study was to determine the effects of TNF, IL-1, IFN-alpha and IFN-alpha on the mRNA levels of the key enzymes involved in fat metabolism in 3T3-F442A adipocytes. TNF, IL-1, IFN-alpha and IFN-gamma decreased lipoprotein lipase activity and increased lipolysis in adipocytes. TNF, IFN-alpha and IFN-gamma decreased fatty acid synthesis while IL-1 increased fatty acid synthesis. However, the cytokine effects on mRNA levels were not always consistent with the observed changes in activity and were unique for each cytokine. Specifically, while all cytokines decreased LPL activity, only TNF and IFN-gamma decreased LPL mRNA levels. In addition, while TNF, IFN-alpha and IFN-gamma decreased fatty acid synthesis, only TNF significantly decreased the mRNA levels of both acetyl CoA carboxylase and fatty acid synthase, the key enzymes in fatty acid synthesis. IFN-alpha and IFN-gamma decreased fatty acid synthase mRNA levels without significantly altering acetyl CoA carboxylase mRNA. IL-1 caused a slight increase in fatty acid synthesis and increased acetyl CoA carboxylase mRNA levels. Finally, while all cytokines increased lipolysis, hormone sensitive lipase mRNA levels were decreased by TNF, IFN-alpha and IFN-gamma treatment. These results indicate that the regulation of adipocyte lipid metabolism by cytokines is complex and that coordinate changes in mRNA levels cannot account for the observed metabolic changes.(ABSTRACT TRUNCATED AT 250 WORDS)
This paper references
10.1056/NEJM199207303270506
Metabolic disturbances and wasting in the acquired immunodeficiency syndrome.
C. Grunfeld (1992)
10.1161/01.ATV.11.3.495
Effect of interleukin-1 on lipid metabolism in the rat. Similarities to and differences from tumor necrosis factor.
K. R. Feingold (1991)
10.1016/0092-8674(74)90116-0
An established pre-adipose cell line and its differentiation in culture.
H. Green (1974)
10.1016/0167-4889(86)90201-6
Regulation of lipoprotein lipase synthesis and 3T3-L1 adipocyte metabolism by recombinant interleukin 1.
S. Price (1986)
Model for cachexia in chronic disease: secretory products of endotoxin-stimulated macrophages induce a catabolic state in 3T3-L1 adipocytes.
P. H. Pekala (1984)
10.1016/0005-2760(89)90246-4
Cachectin/tumor necrosis factor and interleukin-1 show different modes of combined effect on lipoprotein lipase activity and intracellular lipolysis in 3T3-L1 cells.
H. Ogawa (1989)
10.1042/BJ2490765
Regulation of lipoprotein lipase mRNA content in 3T3-L1 cells by tumour necrosis factor.
P. Cornelius (1988)
10.1128/MCB.9.3.974
Transcriptional regulation of acetyl coenzyme A carboxylase gene expression by tumor necrosis factor in 30A-5 preadipocytes.
M. Pape (1989)
10.1093/CLINCHEM/21.9.1245
An analytical system for rapid separation of tissue nucleotides at low pressures on conventional anion exchangers.
J. X. Khym (1975)
10.1016/B978-0-12-152808-9.50011-0
Acetyl coenzyme A carboxylase.
M. Lane (1974)
10.1016/0002-9343(91)80154-E
Circulating interferon-α levels and hypertriglyceridemia in the acquired immunodeficiency syndrome
Carl Grunfeld (1991)
A rapid assay for lipoprotein lipase.
M. Schotz (1970)
10.1016/0092-8674(75)90087-2
An established preadipose cell line and its differentiation in culture II. Factors affecting the adipose conversion
H. Green (1975)
10.1093/OXFORDJOURNALS.JBCHEM.A122990
Inhibitory effect of tumor necrosis factor on gene expression of hormone sensitive lipase in 3T3-L1 adipocytes.
M. Sumida (1990)
10.1016/B978-0-12-768220-4.50019-X
THE HUMAN INTERFERONS: THEIR PURIFICATION AND SEQUENCE, CLONING AND EXPRESSION IN BACTERIA, AND BIOLOGICAL PROPERTIES
S. Pestka (1983)
10.1016/0003-9861(71)90227-X
Acetyl CoA carboxylase. 3. Purification and properties of a biotin carboxyl carrier protein.
A. Nervi (1971)
10.1146/ANNUREV.ME.26.020175.000301
Metabolic response to infection.
W. Beisel (1975)
10.1152/AJPENDO.1992.263.2.E301
Differential effects of interleukin-1 and tumor necrosis factor on ketogenesis.
R. A. Memon (1992)
10.1093/OXFORDJOURNALS.JBCHEM.A121917
Human recombinant TNF suppresses lipoprotein lipase activity and stimulates lipolysis in 3T3-L1 cells.
M. Kawakami (1987)
10.1016/0003-9861(86)90384-X
Regulation of lipoprotein lipase synthesis by recombinant tumor necrosis factor--the primary regulatory role of the hormone in 3T3-L1 adipocytes.
S. Price (1986)
10.2337/diab.41.2.S97
Role of Cytokines in Inducing Hyperlipidemia
K. Feingold (1992)
10.1007/BF02172087
The metabolic effects of tumor necrosis factor and other cytokines
C. Grunfeld (1991)
10.1210/JCEM.74.5.1373735
Lipids, lipoproteins, triglyceride clearance, and cytokines in human immunodeficiency virus infection and the acquired immunodeficiency syndrome.
C. Grunfeld (1992)
10.1210/ENDO-104-5-1383
Regulation of insulin receptors and insulin responsiveness in 3T3-L1 fatty fibroblasts.
F. Karlsson (1979)
10.1172/JCI107855
Selective measurement of two lipase activities in postheparin plasma from normal subjects and patients with hyperlipoproteinemia.
R. Krauss (1974)
10.1128/MCB.8.6.2394
Recombinant human cachectin/tumor necrosis factor but not interleukin-1 alpha downregulates lipoprotein lipase gene expression at the transcriptional level in mouse 3T3-L1 adipocytes.
R. Zechner (1988)
10.1172/JCI113046
Tumor necrosis factor-alpha stimulates hepatic lipogenesis in the rat in vivo.
K. Feingold (1987)
10.1210/MEND-2-5-395
Effect of tumor necrosis factor on acetyl-coenzyme A carboxylase gene expression and preadipocyte differentiation.
M. Pape (1988)
Recombinant interleukin 1 suppresses lipoprotein lipase activity in 3T3-L1 cells.
B. Beutler (1985)
10.1210/ENDO-109-5-1723
Glucocorticoid-induced insulin resistance in vitro: evidence for both receptor and postreceptor defects.
C. Grunfeld (1981)
10.1146/ANNUREV.BI.42.070173.000321
Saturated fatty acid biosynthesis and its regulation.
J. Volpe (1973)
10.1073/PNAS.80.9.2743
Selective inhibition of synthesis of enzymes for de novo fatty acid biosynthesis by an endotoxin-induced mediator from exudate cells.
P. Pekala (1983)
10.1073/PNAS.79.3.912
Lipoprotein lipase suppression in 3T3-L1 cells by an endotoxin-induced mediator from exudate cells.
M. Kawakami (1982)
10.1210/ENDO.130.1.1370149
Stimulation of lipolysis in cultured fat cells by tumor necrosis factor, interleukin-1, and the interferons is blocked by inhibition of prostaglandin synthesis.
K. Feingold (1992)
10.1016/B978-0-12-491220-5.50013-6
Specific molecular activities of recombinant and hybrid leukocyte interferons.
E. Rehberg (1982)
10.3181/00379727-200-43424
Tumor Necrosis Factor, Interleukin, and Interferon Induced Changes in Lipid Metabolism as Part of Host Defense
C. Grunfeld (1992)



This paper is referenced by
10.1515/CCLM.1999.059
Reduction of Circulating Cholesterol and Apolipoprotein Levels during Sepsis
P. Fraunberger (1999)
LIMBIC FOREBRAIN INTERLEUKIN-1β MECHANISMS IN THE CENTRAL REGULATION OF FEEDING, TASTE PERCEPTION AND METABOLISM
G. Takács (2010)
10.31274/ETD-180810-912
Effects of glucagon, glycerol, and glucagon plus glycerol on gluconeogenesis, lipogenesis, and lipolysis in periparturient Holstein cows
Nimer Mehyar (2011)
10.1016/S0079-6123(06)53022-5
Hypothalamic integration of immune function and metabolism
A. Guijarro (2006)
10.1016/j.heares.2015.11.001
Preface
Juichi Ito (2015)
Symposium on ‘ Frontiers in adipose tissue biology ’ Regulation of adipose tissue lipolysis revisited
V. Bézaire (2009)
10.1093/JN/127.9.1917S
Potential Role of TNFα and Lipoprotein Lipase as Candidate Genes for Obesity
P. Kern (1997)
Immunological and behavioral consequences of high-fat diet feeding in mice
D. Lavin (2010)
Complexo miointimal das carótidas comum e interna de portadores de esquistossomose mansônica hepatoesplênica: avaliação com eco- Doppler colorido
A. V. Guimarães (2006)
10.1210/jc.2007-2691
Reciprocal relation between marrow adiposity and the amount of bone in the axial and appendicular skeleton of young adults.
N. Di iorgi (2008)
10.1016/J.BIOCHI.2007.01.003
Differentiation-dependent expression of interferon gamma and toll-like receptor 9 in 3T3-F442A adipocytes.
Wael Khazen (2007)
10.1016/0899-9007(96)90013-1
Regulation of lipid metabolism by cytokines during host defense.
C. Grunfeld (1996)
10.1242/dmm.030411
Adipose tissue and metabolic and inflammatory responses to stroke are altered in obese mice
Michael J Haley (2017)
10.1007/s00109-002-0384-9
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
10.5551/JAT.13.21
The effect of interleukin-1 receptor antagonist on arteries and cholesterol metabolism.
K. Isoda (2006)
10.1016/S0065-2776(08)60428-9
Interferon-gamma: biology and role in pathogenesis.
A. Billiau (1996)
10.1371/journal.pone.0071661
High Fat Diet Accelerates Pathogenesis of Murine Crohn’s Disease-Like Ileitis Independently of Obesity
L. Gruber (2013)
10.1089/THY.1998.8.687
Effects of cytokines on expression of thyrotropin receptor mRNA in rat preadipocytes.
K. Haraguchi (1998)
10.1201/B10264-15
Health Benefits of Kochujang (Korean Red Pepper Paste)
Kun-Young Park (2010)
10.1016/S0167-7306(08)60517-7
Chapter 10 Adipose tissue and lipid metabolism
D. Bernlohr (1996)
10.1016/j.molmet.2020.01.010
Antisense oligonucleotide treatment produces a type I interferon response that protects against diet-induced obesity
Kristin M. McCabe (2020)
10.1160/TH04-03-0199
Differential effects of heparin and low molecular weight heparin on osteoblastogenesis and adipogenesis in vitro.
Stephanie L Osip (2004)
10.1016/j.peptides.2007.03.005
Adrenomedullin is a novel adipokine: Adrenomedullin in adipocytes and adipose tissues
Y. Li (2007)
10.1016/J.BBALIP.2005.02.011
Down-regulated expression of PPARα target genes, reduced fatty acid oxidation and altered fatty acid composition in the liver of mice transgenic for hTNFα
H. Glosli (2005)
10.1016/j.bbagen.2012.07.010
Fatty acid synthase inhibitor orlistat induces apoptosis in T cell lymphoma: role of cell survival regulatory molecules.
S. Kant (2012)
10.1016/j.bbrc.2009.12.145
The acute phase response stimulates the expression of angiopoietin like protein 4.
B. Lu (2010)
10.1186/1472-6793-12-8
Interleukin-1 beta: a potential link between stress and the development of visceral obesity
K. J. Speaker (2012)
10.1002/ptr.2434
Astragaloside IV attenuates lipolysis and improves insulin resistance induced by TNFα in 3T3‐L1 adipocytes
Boren Jiang (2008)
10.1016/S0006-291X(03)01198-7
STAT 1 binds to the LPL promoter in vitro.
J. Hogan (2003)
Dextrose infusion and dramatic deterioration of cerebral malaria:A case report
O. A. Iyamu (2012)
10.1111/j.1749-6632.1999.tb07794.x
On the Control of Lipolysis in Adipocytes
C. Londos (1999)
10.1016/S0163-7827(99)00003-X
Physiological specialisation of adipose tissue.
C. Pond (1999)
See more
Semantic Scholar Logo Some data provided by SemanticScholar