← Back to Search
Development Of A Rabies Vaccine In Cell Culture For Veterinary Use In The Lyophilized Form
Matheus Gonçalves Severo
Published 2013 · Biology
Download PDFAnalyze on Scholarcy
Rabies is a viral zoonosis with 61,000 estimated deaths per year. The rabies vaccine is the best treatment before and after exposure and the virus can be produced in animal cells for veterinary use. Lyophilization has permitted the development of vaccines that would otherwise be too unstable for effective use, including a rabies vaccine that has improved stability when freeze-dried. This chapter is divided into two parts: a review of the rabies virus, viral vaccines, and lyophilization; and a case of study of infected rabies cells kinetics and vaccine freeze-drying with different cryoprotectors. The experiment tested three viral inocula in baby hamster kidney-21 cells growing in 1.5-L spinner flasks, comparing kinetics and virus titration to determine the best inoculum. Harvests of the best inoculum were inactivated and 12 formulations of excipients were made and freeze-dried, comparing visual aspects and residual moisture to select the best formulations.
This paper references
An Unexpected pH Effect on the Stability of Moexipril Lyophilized Powder
R. Strickley (2004)
Dry powders of stable protein formulations from aqueous solutions prepared using supercritical CO(2)-assisted aerosolization.
S. Sellers (2001)
Antibody persistence following preexposure regimens of cell-culture rabies vaccines: 10-year follow-up and proposal for a new booster policy.
A. Strady (1998)
Comparative efficacy of various chemical stabilizers on the thermostability of a live-attenuated peste des petits ruminants (PPR) vaccine.
J. Sarkar (2003)
Effect of freezing on conformation and stability of the virions of southern bean mosaic virus.
O. Sehgal (1975)
Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells.
Stabilization of respiratory syncytial virus (RSV) against thermal inactivation and freeze-thaw cycles for development and control of RSV vaccines and immune globulin.
C. Gupta (1996)
Rabies virus glycoprotein pseudotyping of lentiviral vectors enables retrograde axonal transport and access to the nervous system after peripheral delivery.
N. D. Mazarakis (2001)
Validação de bioensaios para o estudo da imunogenicidade da vacina contra raiva.
Regina Maria Mourão Fuches (2010)
An experimental rabies vaccine produced with a new BHK-21 suspension cell culture process: use of serum-free medium and perfusion-reactor system.
P. Perrin (1995)
The Stabilizing Effect of Sucrose upon Respiratory Syncytial Virus Infectivity
T. Law (1968)
Formulation of Tigecycline Injection by Lyophilization Technique
Y NaveenKrishna. (2012)
Variation of Stoichiometric Ratios and Their Correlation for Monitoring and Control of Animal Cell Cultures
A. Zeng (1998)
GLIMPSES INTO THE REALM OF FREEZE-DRYING : CLASSICAL ISSUES AND NEW VENTURES
L. Rey (1999)
Rabies vaccines produced in cell culture
M. Roumiantzeff (1985)
Cell-culture vaccines for veterinary use. In: Laboratory techniques in rabies
P. RECULARD (1996)
Inactivated rabies vaccine produced from the Flury LEP strain of virus grown in BHK-21 suspension cells.
Walter G Chapman (1973)
Estructura antigenica y mecanismos de infeccion del virus de la rabia (In Spanish)
J.A.M. 1996. HIROSE (1996)
2 – Rabies Virus
W. Wunner (2007)
Heat and mass transfer scale-up issues during freeze-drying, I: Atypical radiation and the edge vial effect
S. Rambhatla (2008)
Lyophilization and the Thermostability of Vaccines
J. Rexroad (2002)
Infection of BHK-21 cells cultivated in stationary monolayers by PV and CVS strains
A. Batista (2009)
Comparison of various culture modes for the production of rabies virus by Vero cells grown on microcarriers in a 2-l bioreactor
K. Trabelsi (2005)
Effect of drying conditions on the quality of vacuum-microwave dried potato cubes
Joanna Bondaruk (2007)
Modeling of the primary and secondary drying stages of the freeze drying of pharmaceutical products in vials: numerical results obtained from the solution of a dynamic and spatially multi-dimensional lyophilization model for different operational policies.
P. Sheehan (1998)
Vaccines. In: Rabies First Edition (A
D. J. BRIGGS (2002)
Rabies: Virus and Disease
S. Nadin-Davis (2015)
Xerovac: an ultra rapid method for the dehydration and preservation of live attenuated Rinderpest and Peste des Petits ruminants vaccines.
E. Worrall (2000)
Canine and feline rabies vaccines, past and present. In: The Natural History of Rabies (G.M
T. O. BUNN (1991)
Nanospray Drying as a Novel Technique for the Manufacturing of Inhalable NSAID Powders
Aquino Rita Patrizia (2014)
Protectants used in the cryopreservation of microorganisms.
Z. Hubálek (2003)
THE ELECTRON MICROSCOPY OF RABIES VIRUS IN CULTURES OF CHICKEN EMBRYO TISSUES.
M. Davies (1963)
A rabies vaccine from hamster kidney tissue cultures: preparation and evaluation in animals.
P. Fenje (1960)
Lyophilization and development of solid protein pharmaceuticals.
W. Wang (2000)
Effect of ultrasonically induced nucleation on ice crystals' size and shape during freezing in vials
Mathieu Saclier (2010)
Vaccines for domestic animals
J. P. Opdebeeck (1994)
The immune response to rabies virus infection and vaccination.
N. Johnson (2010)
Applications of DSC in the development of improved freeze-drying processes for labile biologicals
R. Hatley (1991)
Optimization of virus yield as a strategy to improve rabies vaccine production by Vero cells in a bioreactor.
K. Trabelsi (2006)
Contribution to rabies prevention.
P. Sureau (1992)
Validation of a virus neutralization potency test in BHK-21 cells for rabies immunoglobulins in a two-center study.
Wlamir Corrêa de Moura (2008)
Evaluation of the safety, immunogenicity, and pharmacokinetic profile of a new, highly purified, heat-treated equine rabies immunoglobulin, administered either alone or in association with a purified, Vero-cell rabies vaccine.
J. Lang (1998)
A new antirabies vaccine for human use; clinical and laboratory results using rabies vaccine made from embryonated duck eggs.
F. Peck (1955)
Evaluation of various serum and animal protein free media for the production of a veterinary rabies vaccine in BHK-21 cells.
H. Kallel (2002)
Virus taxonomy: classification and nomenclature of viruses
A. King (2012)
Characterization of vacuum microwave, air and freeze dried carrot slices
T. M. Lin (1998)
Erratum to “Next Generation Sequencing: Potential and Application in Drug Discovery”
N. K. Yadav (2014)
The importance of freezing on lyophilization cycle development
T. Patapoff (2002)
Multiplicity of Infection
S. Abedon (2013)
Introduction. In: Lyophilization: Introduction and basic principles (Informa Healthcare USA Inc., ed.)
T. A. 2003. JENNINGS (2008)
Rational Design of a Stable, Freeze-Dried Virus-Like Particle-Based Vaccine Formulation
R. Lang (2009)
Dye Exclusion Tests for Cell Viability
H. Phillips (1973)
5 – The Freezing Process
J. Levitt (1980)