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Muscle Contraction Accelerates IL-6 MRNA Expression In The Rat Masseter Muscle.

T. Ono, K. Maekawa, S. Watanabe, H. Oka, T. Kuboki
Published 2007 · Chemistry, Medicine, Biology

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OBJECTIVE This study was conducted to determine if interleukin-6 (IL-6) and interleukin-1beta (IL-1beta) mRNA expression increase in response to muscle contraction caused by repetitive electrical stimulation of the rat masseter muscle. METHODS Male Wistar rats weighing 140-160 g were divided randomly into the following three groups: electrical stimulation (ES) group (n=21), carrageenan injection (CI) group (n=24), and ES under dantrolene sodium (muscle relaxant) injection (ESDI) group (n=7). ES or CI was done to the left masseter; and mock ES or mock CI to the right. Muscle tissues on both sides were sampled for total RNA isolation. Real-time RT-PCR was performed, with the cyclophilin A (CypA) mRNA level in each sample as an internal control. Mean relative IL-6 (il-6/cypA) and IL-1beta (il-1beta/cypA) mRNA levels were compared between the experimental and mock-treated sides within each group. RESULTS Mean IL-6/CypA levels in the ES- or CI-treated muscle significantly increased, without any significant incremental change observed in either mock-treated muscle. Interestingly, the increase in the il-6/cypA level caused by the ES was suppressed by the injection of dantrolene sodium in the ESDI group. Furthermore, the mean il-1beta/cypA level in the CI-treated masseter also significantly increased without any significant incremental change observed in the mock-treated muscle. However, there was no significant difference in the mean il-1beta/cypA levels in the masseter between the ES- and the mock-treated sides. CONCLUSIONS These results show that IL-6 mRNA expression in the rat masseter muscle was accelerated by the CI or by repetitive muscle contraction induced by ES. Since the mRNA level of IL-1beta, a well-known proinflammatory cytokine, was not altered by the contraction, the accelerated IL-6 mRNA expression elicited by the muscle contraction does not seem to be related to local inflammation.
This paper references
Histological and neurophysiological changes induced by carrageenan in skeletal muscle of cat and rat
B. Diehl (2005)
Impact of three different types of exercise on components of the inflammatory response
I. Brenner (1999)
Immuno-electron-microscopic localization of basic fibroblast growth factor in the dystrophic mdx mouse masseter muscle
S. Matsuda (2004)
Possible beneficial role of exercise in modulating low‐grade inflammation in the elderly
B. K. Pedersen (2003)
A new animal model for assessing mechanisms and management of muscle hyperalgesia
L. Kehl (2000)
Carbohydrate and the cytokine response to 2.5 h of running.
S. Nehlsen-Cannarella (1997)
Metabolic effects of interleukin‐6 in human splanchnic and adipose tissue
Dorthe Lyngsø (2002)
Appearance of new receptive fields in rat dorsal horn neurons following noxious stimulation of skeletal muscle: a model for referral of muscle pain?
U. Hoheisel (1993)
Exercise‐induced increase in serum interleukin‐6 in humans is related to muscle damage.
H. Bruunsgaard (1997)
IL-6 and TNF-α expression in, and release from, contracting human skeletal muscle
A. Steensberg (2002)
Muscle-derived interleukin-6: lipolytic, anti-inflammatory and immune regulatory effects
B. Pedersen (2003)
Evidence that interleukin‐6 is produced in human skeletal muscle during prolonged running
K. Ostrowski (1998)
Rehabilitation with dental prosthesis can increase cerebral regional blood volume.
I. Miyamoto (2005)
A trauma‐like elevation of plasma cytokines in humans in response to treadmill running
K. Ostrowski (1998)
Muscle contractions induce interleukin‐6 mRNA production in rat skeletal muscles
I. Jonsdottir (2000)
Interleukin-6-deficient mice develop mature-onset obesity
V. Wallenius (2002)
Chewing Stimulates Secretion of Human Salivary Secretory Immunoglobulin A
G. Proctor (2001)
The cytokine response to strenuous exercise.
B. Pedersen (1998)
Distribution and regulation of cyclooxygenase‐2 in carrageenan‐induced inflammation
F. Nantel (1999)
Exercise and IL‐6 infusion inhibit endotoxin‐induced TNF‐α production in humans
R. Starkie (2003)
Skeletal myocytes are a source of interleukin‐6 mRNA expression and protein release during contraction: evidence of fiber type specificity
N. Hiscock (2004)
Dantrolene sodium reduces the enhanced leakage of creatine kinase caused by ethanol, cocaine, and electrical stimulation in isolated fast and slow muscles of rat.
M. Pagala (1997)
Dantrolene Inhibition of Sarcoplasmic Reticulum Ca2+Release by Direct and Specific Action at Skeletal Muscle Ryanodine Receptors*
B. Fruen (1997)
Comparative evolution of muscular dystrophy in diaphragm, gastrocnemius and masseter muscles from old male mdx mice
J. Muller (2004)
The fibre-type composition of the first branchial arch muscles in Carnivora and Primates
A. Rowlerson (2004)
Fatigue and damage to the masseter muscle by prolonged low-frequency stimulation in the rat.
K. Yamasaki (2005)
Human muscle cells express IL-6 via a Ca 2+ -dependent pathway
M. Febbraio (2002)
IL-6 enhances plasma IL-1ra, IL-10, and cortisol in humans.
A. Steensberg (2003)
Production of interleukin‐6 in contracting human skeletal muscles can account for the exercise‐induced increase in plasma interleukin‐6
A. Steensberg (2000)
Initial nociceptive sensitization in carrageenin-induced rat paw inflammation is dependent on amine autacoid mechanisms: electrophysiological and behavioural evidence obtained with a quaternary antihistamine, thiazinamium
A. Neil (2004)
An animal model for human masseter muscle: histochemical characterization of mouse, rat, rabbit, cat, dog, pig, and cow masseter muscle.
A. Tuxen (1990)
Effect of mastication on regional cerebral blood flow in humans examined by positron-emission tomography with ¹⁵O-labelled water and magnetic resonance imaging.
T. Momose (1997)
Amino acid release into the knee joint: key role in nociception and inflammation
N. Lawand (2000)
Specialized Cranial Muscles: How Different Are They from Limb and Abdominal Muscles?
J. Sciote (2003)
Anti‐inflammatory and analgesic effects of paeonol in carrageenan‐evoked thermal hyperalgesia
T. Chou (2003)
Unilateral carrageenan injection into muscle or joint induces chronic bilateral hyperalgesia in rats
R. Radhakrishnan (2003)
Comparison of pain and quality of life in bruxers and patients with myofascial pain of the masticatory muscles.
T. T. Dao (1994)
Contributing factors to chronic myofascial pain: a case–control study
A. Velly (2003)

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