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Short-term Electrical Stimulation Alters Tongue Muscle Fibre Type Composition.
E. Pae, Jon-Philippe K. Hyatt, Jennifer Wu, Patricia Chien
Published 2007 · Medicine, Biology
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OBJECTIVE To examine whether short-term exogenous activation of a tongue muscle induced a phenotypic shift from a fast to a slow fibre-type, and thus assess a potential therapeutic avenue to protect against obstructive sleep apnoea (OSA). METHODS New Zealand White rabbit genioglossus (GG) muscle, characteristically a fast muscle, was continuously stimulated at a frequency attributed to slow muscle (10Hz, 3V DC pulses) using an implanted micro-circuit for 7 days. Changes in muscle fibre types and aerobic capacity were assessed between stimulated and un-stimulated (control) groups using immunohistochemistry and electrophoresis for myosin heavy chain (MHC) and assayed for citrate synthase. RESULTS Compared to the un-stimulated control group, stimulated GG muscles had more (approximately 13%) type I MHC (slow-twitch) content; a proportional decrease in type II MHC (fast-twitch) isoform also occurred in the stimulated GG muscle (P<0.05). Electrophoresis analysis on whole muscle and single fibre MHC showed an increased type I expression in the stimulated GG muscle (P<0.01). A commensurate rise in citrate synthase activity, indicating a change in aerobic capacity, was also observed in the stimulated GG muscles. CONCLUSION Together, these results demonstrate a successful alteration in tongue muscle characteristics using exogenous electrical stimulation and perhaps a potential therapeutic application for OSA.
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