Whey Protein Isolate For The Preparation Of Covalent Immobilization Beads
Marwa I. Wahba, Tarik N. Soliman
Published 2018 · Chemistry
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Abstract Whey protein isolate (WPI) was employed, for the first time, to activate carrageenan (Car) beads for the covalent immobilization of the Aspergillus oryzae β-D-galactosidase (β-gal). These Car beads were subjected to a WPI treatment step followed by a glutaraldehyde (GA) treatment step in order to enable such covalent immobilization. The WPI treatment was optimized via the Box-Behnken Design (BBD). The BBD anticipated that treating the Car beads with a 2.36% WPI solution of pH 5.25 for 7.04 h would allow for the attainment of an immobilized β-gal's activity recovery percent of 34.43%. A verification experiment was accomplished while employing the abovementioned conditions and an immobilized β-gal's activity recovery percent of 34.80 ± 1.11% was attained. It was also shown that the immobilization of β-gal onto the GA-WPI treated Car beads did not alter the enzyme's optimum temperature or optimum pH. Moreover, a reusability study was conducted and 93.84 ± 0.72% of the immobilized β-gal's initial observed activity was preserved during the 13th reusability cycle.
This paper references
Optimisation of immobilisation conditions for chick pea β-galactosidase (CpGAL) to alkylamine glass using response surface methodology and its applications in lactose hydrolysis.
Devesh Kishore (2012)
Agar-carrageenan hydrogel blend as a carrier for the covalent immobilization of β-D-galactosidase
Marwa I. Wahba (2017)
Chondrogenic potential of injectable κ-carrageenan hydrogel with encapsulated adipose stem cells for cartilage tissue-engineering applications.
Elena Geta Popa (2015)
Control of protein immobilization: coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance.
Keven Hernandez (2011)
Whey protein coating increases bilayer rigidity and stability of liposomes in food-like matrices.
Monika Frenzel (2015)
Treated calcium pectinate beads for the covalent immobilization of β-d-galactosidase.
Marwa I. Wahba (2016)
Immobilization of Aspergillus oryzae β-Galactosidase on Cellulose Acetate-Polymethylmethacrylate Membrane and Its Application in Hydrolysis of Lactose from Milk and Whey
Shakeel Ahmed Ansari (2014)
A new heterofunctional amino-vinyl sulfone support to immobilize enzymes: Application to the stabilization of β-galactosidase from Aspergillus oryzae
Hadjer Zaak (2018)
pH-Dependent Selective Protein Adsorption into Mesoporous Silica
Sebastian T. Moerz (2015)
Inorganic Materials as Supports for Covalent Enzyme Immobilization: Methods and Mechanisms
Paolo Zucca (2014)
Immobilization of Aspergillus oryzae β galactosidase on zinc oxide nanoparticles via simple adsorption mechanism.
Qayyum Husain (2011)
SYNTHESIS OF A BIOCOPOLYMER CARRAGEENAN-g-POLY(AAm-co-IA)/ MONTMORILONITE SUPERABSORBENT HYDROGEL COMPOSITE
Mohammad Sadeghi (2012)
Chemical Modification in the Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities.
Nazzoly Rueda (2016)
Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization
Oveimar Barbosa (2014)
Modifying enzyme activity and selectivity by immobilization.
Rafael C Rodrigues (2013)
Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases
Urszula Guzik (2014)
Covalent Immobilization of β-Galactosidase on Carrageenan Coated with Chitosan
Magdy Elnashar (2009)
Galactans: an overview of their most important sourcing and applications as natural polysaccharides
Cédric Delattre (2011)
Characterising the secondary structure changes occurring in high density systems of BLG dissolved in aqueous pH 3 buffer
Jc Ioannou (2015)
Potential Applications of Immobilized β-Galactosidase in Food Processing Industries
Parmjit S. Panesar (2010)
Importance of the Support Properties for Immobilization or Purification of Enzymes
Jose C.S. dos Santos (2015)
Review Article: Immobilized Molecules Using Biomaterials and Nanobiotechnology
Magdy Elnashar (2010)
Controlling the porosity and microarchitecture of hydrogels for tissue engineering.
Nasim Annabi (2010)
Characterization of protein adsorption onto silica nanoparticles: influence of pH and ionic strength
Jens Meissner (2015)
Immobilization of Aspergillus oryzae β galactosidase on concanavalin A-layered calcium alginate-cellulose beads and its application in lactose hydrolysis in continuous spiral bed reactors
Shakeel Ahmed Ansari (2011)
Electrostatically driven protein aggregation: beta-lactoglobulin at low ionic strength.
Pinaki R. Majhi (2006)
In vitro impact of a whey protein isolate (WPI) and collagen hydrolysates (CHs) on B16F10 melanoma cells proliferation.
Gilson Araújo Castro (2009)
Cheese whey: A potential resource to transform into bioprotein, functional/nutritional proteins and bioactive peptides.
Jay Shankar Singh Yadav (2015)
pH effects on the molecular structure of β-lactoglobulin modified air-water interfaces and its impact on foam rheology.
Kathrin Engelhardt (2013)
Improvement of enzyme activity, stability and selectivity via immobilization techniques
César Mateo (2007)
Lactose Hydrolysis by β-Galactosidase Covalently Immobilized to Thermally Stable Biopolymers
Magdy Elnashar (2009)
Effect of the immobilization protocol on the properties of lipase B from Candida antarctica in organic media: Enantiospecifc production of atenolol acetate
Oveimar Barbosa (2011)
Exploiting the Versatility of Aminated Supports Activated with Glutaraldehyde to Immobilize β-galactosidase from Aspergillus oryzae
Hadjer Zaak (2017)
Porous chitosan beads of superior mechanical properties for the covalent immobilization of enzymes.
Marwa I. Wahba (2017)
Nylon 6 film and nanofiber carriers: Preparation and laccase immobilization performance
Enrico Fatarella (2014)
Whey protein coating bead improves the survival of the probiotic Lactobacillus rhamnosus CRL 1505 to low pH.
Carla Luciana Gerez (2012)
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.
Isabelle Migneault (2004)
Application of Plackett-Burman Screening Design to the Modeling of Grafted Alginate-Carrageenan Beads for the Immobilization of Penicillin G Acylase
Magdy Elnashar (2014)
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