Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Induction Of Protein Oxidation By Cobalt And Chromium Ions In Human U937 Macrophages.

A. Petit, F. Mwale, C. Tkaczyk, J. Antoniou, D. Zukor, O. Huk
Published 2005 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Metal particles and ions from hip prostheses have the potential to induce the production of reactive oxygen species (ROS), making them prime suspects for disturbing the cellular balance of oxidants/antioxidants (redox state of the cell). To better understand the cellular effect of metal ions from metal-on-metal prostheses, the aim of this study was to examine the effect of cobalt (Co2+) and chromium (Cr3+) ions on protein oxidation in human U937 macrophages. Protein oxidation was measured by Western blot using antibodies directed against dinitrophenylhydrazine (DNP)-derivatized protein carbonyls, the most commonly measured products of protein oxidation in biological samples. Three DNP-derived proteins were detected. The first has a molecular weight of 16 kDa and is expressed at a very low level. The second has a molecular weight of 48 kDa and its level is not regulated by metal ions. The third is a 69 kDa protein and its level is regulated by Co2+ and Cr3+ ions. Therefore, the last band served as a marker of protein oxidation in the present study. Results showed that Co2+ and Cr3+ ions induced a time- and dose-dependent protein oxidation reaching 6.5 and 2.9 times the control after 72 h, respectively, which were inhibited by the antioxidant glutathione monoethyl-ester. Finally, results showed that the oxidized proteins are mainly found in the cytoplasmic fraction of the cells and are absent from the nucleus. In conclusion, our results suggest that metal ions from metal-on-metal prostheses have the potential to modify the redox state of cells both locally (periprosthetic environment) or systematically (circulating cells). The long term effect of these ions on protein oxidation in vivo remains to be investigated.
This paper references
10.1016/J.ARTH.2004.09.011
Induction of apoptosis and necrosis by metal ions in vitro.
O. Huk (2004)
10.1016/S0142-9612(02)00616-6
Effects of transforming growth factor-beta 1 (TGF-beta1) on in vitro mineralization of human osteoblasts on implant materials.
H. Zhang (2003)
10.2106/00004623-199409000-00009
Migration of corrosion products from modular hip prostheses. Particle microanalysis and histopathological findings.
R. Urban (1994)
10.2106/00004623-199306000-00004
The differences in toxicity and release of bone-resorbing mediators induced by titanium and cobalt-chromium-alloy wear particles.
D. Haynes (1993)
10.1016/S0891-5849(02)00809-2
Oxidative DNA and protein damage in metal-induced toxicity and carcinogenesis.
K. Kasprzak (2002)
10.1002/JOR.1100140107
Role of tumor necrosis factor alpha in particulate‐induced bone resorption
S. M. Algan (1996)
10.1172/JCI107236
Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent.
B. Babior (1973)
10.2106/00004623-200006000-00005
Total Hip Arthroplasty with Use of the Metasul Metal-on-Metal Articulation: Four to Seven-Year Results*
L. Dorr (2000)
10.1002/1615-9861(200201)2:1<3::AID-PROT3>3.0.CO;2-R
Two‐dimensional gel electrophoresis in proteomics: Old, old fashioned, but it still climbs up the mountains
T. Rabilloud (2002)
Chemicals in diesel exhaust particles generate reactive oxygen radicals and induce apoptosis in macrophages.
T. Hiura (1999)
10.1302/0301-620X.83B8.12755
Chromosomal changes after surgery for joint replacement.
C. Case (2001)
10.1097/00003086-199312000-00019
Biocompatibility of polyethylene and host response to loosening of cementless total hip replacement.
S. Santavirta (1993)
10.5301/HIP.2008.3859
In vivo tribological performance of 231 metal-on-metal hip articulations.
C. Rieker (2002)
10.1097/00003086-199608001-00022
Cobalt and Chromium Concentrations in Patients With Metal on Metal Total Hip Replacements
J. Jacobs (1996)
10.1016/0142-9612(84)90024-3
In vivo production of hexavalent chromium.
G. T. Rogers (1984)
10.1016/0304-4173(86)90005-4
The O2- -forming NADPH oxidase of the phagocytes: nature, mechanisms of activation and function.
F. Rossi (1986)
10.1081/DMR-100102336
QUANTIFICATION AND SIGNIFICANCE OF PROTEIN OXIDATION IN BIOLOGICAL SAMPLES*
E. Shacter (2000)
10.1302/0301-620x.84b1.11324
Adverse tissue reactions to wear particles from Co-alloy articulations, increased by alumina-blasting particle contamination from cementless Ti-based total hip implants. A report of seven revisions with early failure.
M. Boehler (2002)
10.1016/S0891-5849(97)00295-5
The critical role of Hepes in SIN-1 cytotoxicity, peroxynitrite versus hydrogen peroxide.
E. E. Lomonosova (1998)
10.1023/A:1008878527233
Cell death induced by metal ions: necrosis or apoptosis?
D. Granchi (1998)
10.1097/00003086-199411000-00018
Etiology of Osteolysis Around Porous‐Coated Cementless Total Hip Arthroplasties
M. Jasty (1994)
10.1016/J.BIOMATERIALS.2004.08.004
Quantitative analysis of macrophage apoptosis vs. necrosis induced by cobalt and chromium ions in vitro.
I. Catelas (2005)
10.1016/S0300-483X(02)00378-5
Cytotoxicity and oxidative mechanisms of different forms of chromium.
D. Bagchi (2002)
10.1006/TAAP.1996.0330
Stimulation of human and rat alveolar macrophages by urban air particulates: effects on oxidant radical generation and cytokine production.
S. Becker (1996)
10.1016/S0945-053X(01)00188-3
Non-enzymatic covalent modifications of proteins: mechanisms, physiological consequences and clinical applications.
P. Cloos (2002)
10.1097/01.BLO.0000043066.62337.9D
Metal-on-Metal Versus Polyethylene in Hip Arthroplasty: A Randomized Clinical Trial
S. Macdonald (2003)
10.1152/AJPLUNG.1996.271.6.L932
IL-1 beta stimulates superoxide and delayed peroxynitrite production by pulmonary vascular smooth muscle cells.
A. Boota (1996)
10.1093/CLINCHEM/47.2.275
Development of a routine method for the determination of trace metals in whole blood by magnetic sector inductively coupled plasma mass spectrometry with particular relevance to patients with total hip and knee arthroplasty.
C. Case (2001)
10.1152/AJPLUNG.1997.272.5.L979
Endothelial barrier dysfunction and p42 oxidation induced by TNF-alpha are mediated by nitric oxide.
T. Ferro (1997)
The role of chromium(V) in the mechanism of chromate-induced oxidative DNA damage and cancer.
Kent D. Sugden (2000)
Pathology of the bone-implant interfaces.
N. Al-Saffar (1999)
10.1146/ANNUREV.IMMUNOL.15.1.351
Redox regulation of cellular activation.
H. Nakamura (1997)
10.2165/00003088-199630050-00004
Clinical Pharmacokinetics of Molsidomine
B. Rosenkranz (1996)
10.1007/978-1-59259-768-0_9
Clinical Applications of Proteomics
E. Petricoin (2003)
10.1016/S0736-0266(01)00077-8
Differential apoptotic response of J774 macrophages to alumina and ultra‐high‐molecular‐weight polyethylene particles
A. Petit (2002)
10.1016/0014-5793(94)01056-0
Intense oxidative DNA damage promoted by l‐DOPA and its metabolites implications for neurodegenerative disease
J. Spencer (1994)
10.1016/0891-5849(94)00159-H
Oxidative mechanisms in the toxicity of metal ions.
S. Stohs (1995)
10.1007/BF00739271
Transformation-induced plasticity in Fe-17.20% Cr-7.34% Ni steel
K. Wakasa (1978)
10.1161/01.RES.0000093221.98213.E0
Identification of novel signaling complexes by functional proteomics.
P. Ping (2003)
Nitrated and oxidized plasma proteins in smokers and lung cancer patients.
B. Pignatelli (2001)
10.2106/00004623-200103000-00017
Metal sensitivity in patients with orthopaedic implants.
N. Hallab (2001)
10.1016/S1081-1206(10)62884-0
Enhancement of allergic inflammation by diesel exhaust particles: permissive role of reactive oxygen species.
A. Casillas (1999)
10.1023/A:1012800813662
Cytotoxic and apoptotic effects of cobalt and chromium ions on J774 macrophages – Implication of caspase-3 in the apoptotic pathway
I. Catelas (2001)
10.1159/000078765
T Lymphocyte-Mediated Immune Responses to Chemical Haptens and Metal Ions: Implications for Allergic and Autoimmune Disease
S. Martin (2004)
10.1046/j.1365-2133.2003.05404.x
Metal sensitivities and orthopaedic implants revisited: the potential for metal allergy with the new metal‐on‐metal joint prostheses
D. Gawkrodger (2003)
10.1016/S0142-9612(00)00041-7
Apoptosis in peri-implant tissue.
S. Stea (2000)
10.1016/S0891-5849(02)00809-2
Serial review: oxidative DNA damage and repairOxidative DNA and protein damage in metal-induced toxicity and carcinogenesis1,3
K. Kasprzak (2002)
10.1016/S1567-5769(01)00180-1
Immunomodulation by metals.
D. Lawrence (2002)
10.1016/S0142-9612(02)00351-4
TNF-alpha secretion and macrophage mortality induced by cobalt and chromium ions in vitro-qualitative analysis of apoptosis.
I. Catelas (2003)
10.1021/TX00024A003
Is there a role for reactive oxygen species in the mechanism of chromium(VI) carcinogenesis?
A. Standeven (1991)
10.1016/S0883-5403(99)90051-7
Cancer incidence in Finnish hip replacement patients from 1980 to 1995: a nationwide cohort study involving 31,651 patients.
P. Paavolainen (1999)
10.1073/PNAS.88.23.10540
Excess brain protein oxidation and enzyme dysfunction in normal aging and in Alzheimer disease.
C. Smith (1991)
10.1302/0301-620X.57B4.444
A study on the effects of particulate metals of orthopaedic interest on murine macrophages in vitro.
T. Rae (1975)
10.1023/A:1013630401959
The cytotoxicity of chromium in osteoblasts: effects on macromolecular synthesis
J. Ning (2002)
10.1016/J.BIOMATERIALS.2003.08.040
Effect of cobalt and chromium ions on bcl-2, bax, caspase-3, and caspase-8 expression in human U937 macrophages.
A. Petit (2004)
10.1016/0014-5793(95)00365-G
Strand scission in DNA induced by l‐DOPA in the presence of Cu(II)
S. Husain (1995)
10.1002/JOR.1100150107
Human monocyte/macrophage response to cobalt‐chromium corrosion products and titanium particles in patients with total joint replacements
S. H. Lee (1997)
10.1073/PNAS.81.15.4732
Radioprotection by glutathione ester: transport of glutathione ester into human lymphoid cells and fibroblasts.
V. P. Wellner (1984)



This paper is referenced by
10.5772/25826
Evidence Linking Elevated Oxidative Stress and Aseptic Loosening of Hip Arthroplasty
Plamen Kinov (2012)
10.1093/jrr/rrs086
Radioprotective effect on HepG2 cells of low concentrations of cobalt chloride: induction of hypoxia-inducible factor-1 alpha and clearance of reactive oxygen species
Wensen Jin (2013)
10.2174/156802611798040787
Interactions of metal ions with DNA, its constituents and derivatives, which may be relevant for anticancer research.
I. Turel (2011)
10.1002/jbm.a.36567
Electrochemical potential zone of viability on CoCrMo surfaces is affected by cell type: Macrophages under cathodic bias are more resistant to killing.
Michael J Wiegand (2019)
10.1002/mabi.200800359
Adhesion of human U937 monocytes to nitrogen-rich organic thin films: novel insights into the mechanism of cellular adhesion.
Pierre-Luc Girard-Lauriault (2009)
10.1016/j.biomaterials.2013.11.014
Osteogenic differentiation of bone marrow MSCs by β-tricalcium phosphate stimulating macrophages via BMP2 signalling pathway.
Zetao Chen (2014)
10.1007/s10953-016-0512-8
Spectrophotometric Study of Complex Formation Between Curcumin and Cr(III) Ion: A Case of Heavily Overlapping Absorption Peaks
Kanitta Phongarthit (2016)
10.1016/j.scitotenv.2012.06.009
Cobalt metabolism and toxicology--a brief update.
L. Simonsen (2012)
10.1016/J.MSEC.2019.109932
Facile synthesized novel hybrid graphene oxide/cobalt ferrite magnetic nanoparticles based surface coating material inhibit bacterial secretion pathway for antibacterial effect.
T. Arun (2019)
10.1016/B978-044453071-4/50012-6
Redox chemistry and biological activities of chromium(III) complexes
A. Levina (2007)
Molecular Mechanisms Involved in Interleukin-1β Release by Macrophages Exposed to Metal Ions from Implantable Biomaterials
Zeina Salloum (2018)
Ions released from metal-on-metal hip implants: «in vitro» and «in vivo» investigations
C. Tkaczyk (2010)
10.1007/s10753-020-01206-z
Titanium Ions Play a Synergistic Role in the Activation of NLRP3 Inflammasome in Jurkat T Cells
X. Li (2020)
10.1016/J.BIOTRI.2019.100091
Tribocorrosion of Polyethylene/Cobalt Contact Combined with Real-Time Fluorescence Assays on Living Macrophages: Development of A Multidisciplinary Biotribocorrosion Device
A. Impergre (2019)
10.1038/srep05729
Molecular analysis of chromium and cobalt-related toxicity
Brian Scharf (2014)
10.1002/jbm.a.34246
Biological responses of human bone marrow mesenchymal stem cells to Sr-M-Si (M = Zn, Mg) silicate bioceramics.
Meili Zhang (2012)
10.1016/j.fct.2020.111707
GENOTOXIC PROPERTIES OF MATERIALS USED FOR ENDOPROSTHESES: EXPERIMENTAL AND HUMAN DATA.
Hong-Min Qin (2020)
10.3390/LUBRICANTS3030539
In Vitro Analyses of the Toxicity, Immunological, and Gene Expression Effects of Cobalt-Chromium Alloy Wear Debris and Co Ions Derived from Metal-on-Metal Hip Implants
O. M. Posada (2015)
10.1007/s10904-011-9611-6
Histidine-Assisted Synthesis and Cellular Compatibility of Magnetic Cobalt Oxide Nanoparticles at Room Temperature
J. Zhao (2011)
10.1039/C4RA02481H
Effects of iron or manganese doping of ZnO nanoparticles on their dissolution, ROS generation and cytotoxicity
Hong Yin (2014)
The Effect of Nanoscale and Micron Sized Alumina and Cobalt Chromium Particles on Plasma Membrane Integrity
Ruth Craven (2016)
10.1016/J.MOLLIQ.2019.02.065
Nonlinear optical properties (NLO) and metal ions sensing responses of a polymer containing 1,3,4-oxadiazole and bisphenol A units
Mihaela Homocianu (2019)
10.1016/J.SNB.2014.12.056
Studies on UV–vis and fluorescence changements in Co2+ and Cu2+ recognition by a new benzimidazole–benzothiadiazole derivative
O. Çimen (2015)
10.1002/bab.1401
Regulatory role of B‐cell maturation antigen on the toxic effect of chromium ions on human SaOS‐2 osteoblasts
D. Li (2017)
ngineered cobalt oxide nanoparticles readily enter cells
lena Papisa (2009)
10.1016/j.biomaterials.2008.09.055
The molecular structure of complexes formed by chromium or cobalt ions in simulated physiological fluids.
C. Tkaczyk (2009)
10.1007/s11010-005-8210-7
Conference overview: Molecular mechanisms of metal toxicity and carcinogenesis
Jacquelyn J. Bower (2005)
Cellular internalisation of dissolved cobalt ions from ingested CoFe2O4 nanoparticles: in vivo experimental evidence
S. Trieste (2012)
10.1007/978-3-030-03742-0_5
Chromium in Health and Longevity
Ruslana Iskra (2018)
10.1080/21691401.2018.1468767
Human macrophage responses to metal-oxide nanoparticles: a review
Camila Figueiredo Borgognoni (2018)
10.1002/jbm.a.34091
Distribution of metal released from cobalt-chromium alloy orthopaedic wear particles implanted into air pouches in mice.
G. Afolaranmi (2012)
AN OVERVIEW ON USES OF ZINC OXIDE NANOPARTICLES
P. S. Bedi (2015)
See more
Semantic Scholar Logo Some data provided by SemanticScholar