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Structural Characterization Of A Sulfated Glucan Isolated From The Aqueous Extract Of Hedysarum Polybotrys Hand.-Mazz

Yikai Shi, L. Zhao, X. Liu, Fangdi Hu, F. Cui, Yingyan Bi, Yufeng Ma, Shilan Feng
Published 2012 · Chemistry

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Abstract A water-soluble sulfated glucan, SHG, was isolated from Hedysarum polybotrys Hand.-Mazz using anion-exchange and gel-permeation chromatography. Elemental analysis indicated that SHG was a sulfated polysaccharide containing small amount of sulfate groups (1.47 mass%). The molecular weight was estimated to be 1.72 × 10 5  Da by high-performance gel permeation chromatography (HPGPC) and size exclusion chromatography–multi angle laser light scattering (SEC–MALLS). SHG took random coil compact conformation in 0.1 M NaNO 3 . Compositional analysis revealed that SHG was composed of glucose only. On the base of partial acid hydrolysis, methylation analysis, gas chromatography–mass spectrometry (GC–MS), Fourier transform infrared (FT-IR) spectra, nuclear magnetic resonance (NMR) spectroscopy of 1 H, 13 C, β-correlation spectroscopy (β-COSY), total correlation spectroscopy (TOCSY), heteronuclear single quantum coherence (HSQC), and heteronuclear multiple bond correlation (HMBC), the results showed that SHG was composed of α- d -(1 → 4)-linked glucopyranosyl residues, with branches at C-6 consisting of non-reducing terminal approximately every eight residues. Sulfate groups may attach to the backbone at O-6, occasionally occurring per thirty-eight anhydrous glucose units.
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