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The Effect Of RGS12 On PDGFbeta Receptor Signalling To P42/p44 Mitogen Activated Protein Kinase In Mammalian Cells.

B. Sambi, M. Hains, C. Waters, M. Connell, F. Willard, A. Kimple, S. Pyne, D. Siderovski, N. Pyne
Published 2006 · Biology, Medicine

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We have previously shown that the PDGFbeta receptor uses a classical GPCR-mediated pathway in order to induce efficient activation of p42/p44 MAPK in response to PDGF. We therefore, considered the possibility that GTPase accelerating proteins (RGS proteins), which regulate GPCR signalling, modulate PDGFbeta receptor-mediated signal transmission. Several lines of evidence were obtained to support functional interaction between the PDGFbeta receptor and RGS12 in HEK 293 and airway smooth muscle cells. Firstly, the over-expression of the RGS12 PDZ/PTB domain N-terminus or RGS12 PTB domain reduced the PDGF-induced activation of p42/p44 MAPK. Secondly, the RGS12 PDZ/PTB domain N-terminus and RGS12 PDZ domain can form a complex with the PDGFbeta receptor. Therefore, the results presented here provide the first evidence to support the concept that the PDZ/PTB domain N-terminus and/or the PTB domain of RGS12 may modulate PDGFbeta receptor signalling. In airway smooth muscle cells, over-expressed recombinant RGS12 and the isolated PDZ/PTB domain N-terminus co-localised with PDGFbeta receptor in cytoplasmic vesicles. To provide additional evidence for a role of the PDZ/PTB domain N-terminus, we used RGS14. RGS14 has the same C-terminal domain architecture of an RGS box, tandem Ras-binding domains (RBDs) and GoLoco motif as RGS12, but lacks the PDZ/PTB domain N-terminus. In this regard, RGS14 exhibited a different sub-cellular distribution compared with RGS12, being diffusely distributed in ASM cells. These findings suggest that RGS12 via its PDZ/PTB domain N-terminus may regulate trafficking of the PDGFbeta receptor in ASM cells.
This paper references
10.1016/0006-2952(93)90132-G
Bradykinin stimulates phospholipase D in primary cultures of guinea-pig tracheal smooth muscle.
S. Pyne (1993)
10.1074/JBC.270.28.16495
Gβγ Subunits Mediate Mitogen-activated Protein Kinase Activation by the Tyrosine Kinase Insulin-like Growth Factor 1 Receptor (*)
L. Luttrell (1995)
10.1006/BBRC.1997.6537
Molecular cloning and expression analysis of rat Rgs12 and Rgs14.
B. Snow (1997)
10.1016/S0092-8674(00)80204-4
Structure of RGS4 Bound to AlF4 −-Activated Giα1: Stabilization of the Transition State for GTP Hydrolysis
J. Tesmer (1997)
10.1128/MCB.18.10.5780
Regulation of Myogenesis by Fibroblast Growth Factors Requires Beta-Gamma Subunits of Pertussis Toxin-Sensitive G Proteins
Y. V. Fedorov (1998)
10.1074/jbc.273.28.17749
GTPase Activating Specificity of RGS12 and Binding Specificity of an Alternatively Spliced PDZ (PSD-95/Dlg/ZO-1) Domain*
B. Snow (1998)
10.1074/JBC.273.52.34687
The N-terminal Domain of RGS4 Confers Receptor-selective Inhibition of G Protein Signaling*
W. Zeng (1998)
10.1042/0264-6021:3370171
Platelet-derived-growth-factor stimulation of the p42/p44 mitogen-activated protein kinase pathway in airway smooth muscle: role of pertussis-toxin-sensitive G-proteins, c-Src tyrosine kinases and phosphoinositide 3-kinase.
A. Conway (1999)
10.1074/JBC.274.46.33097
Characterization of four mammalian numb protein isoforms. Identification of cytoplasmic and membrane-associated variants of the phosphotyrosine binding domain.
Dho Se (1999)
10.1038/35047093
Tyrosine-kinase-dependent recruitment of RGS12 to the N-type calcium channel
M. L. Schiff (2000)
10.1128/MCB.20.22.8352-8363.2000
Platelet-Derived Growth Factor Receptor Association with Na+/H+ Exchanger Regulatory Factor Potentiates Receptor Activity
Stuart Maudsley (2000)
10.1074/jbc.C000509200
Stabilization of the GDP-bound Conformation of Giα by a Peptide Derived from the G-protein Regulatory Motif of AGS3*
Y. Peterson (2000)
10.1093/emboj/20.16.4414
Functional relevance of the disulfide‐linked complex of the N‐terminal PDZ domain of InaD with NorpA
M. Kimple (2001)
10.1038/35059138
Structural determinants for regulation of phosphodiesterase by a G protein at 2.0 Å
K. Slep (2001)
10.1074/JBC.M102771200
Tethering of the platelet-derived growth factor beta receptor to G-protein-coupled receptors. A novel platform for integrative signaling by these receptor classes in mammalian cells.
F. Alderton (2001)
10.1074/JBC.M103208200
RGS12 and RGS14 GoLoco Motifs Are GαiInteraction Sites with Guanine Nucleotide Dissociation Inhibitor Activity*
R. Kimple (2001)
10.1074/JBC.M005291200
Selective Interaction of AGS3 with G-proteins and the Influence of AGS3 on the Activation State of G-proteins*
M. Bernard (2001)
10.1091/MBC.12.3.615
GIPC and GAIP form a complex with TrkA: a putative link between G protein and receptor tyrosine kinase pathways.
X. Lou (2001)
10.1038/nrd747
Regulators of G-Protein signalling as new central nervous system drug targets
R. Neubig (2002)
10.1038/416878a
Structural determinants for GoLoco-induced inhibition of nucleotide release by Gα subunits
R. Kimple (2002)
10.1128/MCB.22.12.4334-4345.2002
RGS12TS-S Localizes at Nuclear Matrix-Associated Subnuclear Structures and Represses Transcription: Structural Requirements for Subnuclear Targeting and Transcriptional Repression
T. Chatterjee (2002)
10.1096/fj.02-0340fje
Pericyte‐specific expression of Rgs5: implications for PDGF and EDG receptor signaling during vascular maturation
H. Cho (2003)
10.1074/JBC.M210371200
Src-mediated RGS16 Tyrosine Phosphorylation Promotes RGS16 Stability*
A. Derrien (2003)
10.1042/BJ20021769
The aorta and heart differentially express RGS (regulators of G-protein signalling) proteins that selectively regulate sphingosine 1-phosphate, angiotensin II and endothelin-1 signalling.
H. Cho (2003)
10.1074/JBC.M208560200
Sphingosine 1-Phosphate and Platelet-derived Growth Factor (PDGF) Act via PDGFβ Receptor-Sphingosine 1-Phosphate Receptor Complexes in Airway Smooth Muscle Cells*
C. Waters (2003)
10.1091/MBC.E04-03-0239
The Na+/H+ exchanger regulatory factor stabilizes epidermal growth factor receptors at the cell surface.
C. Lazar (2004)
10.1016/S0076-6879(04)89019-0
Purification and in vitro functional analysis of the Arabidopsis thaliana regulator of G-protein signaling-1.
F. Willard (2004)
10.1016/S0076-6879(04)89005-0
Application of RGS box proteins to evaluate G-protein selectivity in receptor-promoted signaling.
M. Hains (2004)
10.1016/J.SEMCDB.2003.12.020
The role of G-protein coupled receptors and associated proteins in receptor tyrosine kinase signal transduction.
C. Waters (2004)
10.1074/JBC.M407409200
The RGS14 GoLoco Domain Discriminates among Gαi Isoforms*
V. Mittal (2004)
10.1016/J.CELLSIG.2004.07.011
c-Src is involved in regulating signal transmission from PDGFbeta receptor-GPCR(s) complexes in mammalian cells.
C. Waters (2005)
10.1016/J.AB.2005.02.015
A direct fluorescence-based assay for RGS domain GTPase accelerating activity.
F. Willard (2005)
10.1074/jbc.M406607200
RGS12 Interacts with the SNARE-binding Region of the Cav2.2 Calcium Channel*
Ryan W Richman (2005)



This paper is referenced by
10.21037/atm-20-5729
Macrophage regulator of G-protein signaling 12 contributes to inflammatory pain hypersensitivity.
G. Yuan (2021)
10.2218/gtopdb/f891/2021.2
Regulators of G protein Signaling (RGS) proteins in GtoPdb v.2021.2
Katelin E. Ahlers-Dannen (2021)
10.2218/gtopdb/f891/2020.5
Regulators of G protein Signaling (RGS) proteins (version 2020.5) in the IUPHAR/BPS Guide to Pharmacology Database
Katelin E. Ahlers-Dannen (2020)
10.1016/j.cellsig.2020.109765
Regulator of G protein signaling 10: Structure, expression and functions in cellular physiology and diseases.
Faris Almutairi (2020)
10.7554/eLife.42951
Regulator of G protein signaling 12 enhances osteoclastogenesis by suppressing Nrf2-dependent antioxidant proteins to promote the generation of reactive oxygen species
A. Ng (2019)
10.1038/s41386-019-0423-7
Role of RGS12 in the differential regulation of kappa opioid receptor-dependent signaling and behavior
J. D. Gross (2019)
10.1371/journal.pone.0216167
A role for Regulator of G protein Signaling-12 (RGS12) in the balance between myoblast proliferation and differentiation
Adam B. Schroer (2019)
Regulator of G protein signaling 12 enhances osteoclastogenesis by suppressing Nrf 2-dependent antioxidant proteins to promote the generation of reactive oxygen species
Andrew Ying Hui Ng (2019)
10.2218/gtopdb/f891/2019.4
Regulators of G protein Signaling (RGS) proteins (version 2019.4) in the IUPHAR/BPS Guide to Pharmacology Database
Mohammed Alqinyah (2019)
10.1124/pr.117.015354
Genetic Analysis of Rare Human Variants of Regulators of G Protein Signaling Proteins and Their Role in Human Physiology and Disease
Katherine E Squires (2018)
10.1101/470542
Rgs12 enhances osteoclastogenesis by suppressing Nrf2 activity and promoting the formation of reactive oxygen species
A. Ng (2018)
10.1124/mol.118.111674
RGS10 Regulates the Expression of Cyclooxygenase-2 and Tumor Necrosis Factor Alpha through a G Protein–Independent Mechanism
Mohammed Alqinyah (2018)
10.1158/0008-5472.CAN-17-0669
RGS12 Is a Novel Tumor-Suppressor Gene in African American Prostate Cancer That Represses AKT and MNX1 Expression.
Yongquan Wang (2017)
10.3390/molecules22030344
Sphingosine 1-Phosphate Receptor 1 Signaling in Mammalian Cells
N. Pyne (2017)
10.3389/fcell.2016.00049
ERK Signals: Scaffolding Scaffolds?
B. Casar (2016)
10.1016/bs.ircmb.2015.02.001
Regulators of G-protein-signaling proteins: negative modulators of G-protein-coupled receptor signaling.
G. Woodard (2015)
10.1038/cdd.2015.45
Regulators of G protein signaling 12 promotes osteoclastogenesis in bone remodeling and pathological bone loss
X. Yuan (2015)
10.1038/cddis.2014.98
Overcoming inherent resistance to histone deacetylase inhibitors in multiple myeloma cells by targeting pathways integral to the actin cytoskeleton
S. Mithraprabhu (2014)
G-protein coupled receptor mediated signaling pathways in chemoresistant melanoma and ovarian cancer
Molly K. Altman (2014)
10.1038/tpj.2014.40
Genes involved in pericyte-driven tumor maturation predict treatment benefit of first-line FOLFIRI plus bevacizumab in patients with metastatic colorectal cancer
N. Volz (2014)
10.1002/9781118531426.CH5
Lysophospholipid receptor signalling platforms: the receptor tyrosine kinase-G-protein coupled receptor signaling complex
N. Pyne (2012)
10.1159/000337263
Neuronal Functions of Activators of G Protein Signaling
M. Tse (2012)
10.1371/journal.pone.0028504
An RGS4-Mediated Phenotypic Switch of Bronchial Smooth Muscle Cells Promotes Fixed Airway Obstruction in Asthma
G. Damera (2012)
10.1111/j.1748-1716.2011.02327.x
Group II activators of G‐protein signalling and proteins containing a G‐protein regulatory motif
J. B. Blumer (2012)
10.1016/j.tips.2011.04.002
Receptor tyrosine kinase-G-protein-coupled receptor signalling platforms: out of the shadow?
N. Pyne (2011)
10.1016/B978-0-12-385952-5.00003-8
Role of PDZ proteins in regulating trafficking, signaling, and function of GPCRs: means, motif, and opportunity.
G. Romero (2011)
10.1016/j.cellsig.2009.10.005
RGS14 is a multifunctional scaffold that integrates G protein and Ras/Raf MAPkinase signalling pathways.
Feng-jue Shu (2010)
10.1371/journal.pgen.1001020
Regulator of G Protein Signaling 3 Modulates Wnt5b Calcium Dynamics and Somite Patterning
Christina M. Freisinger (2010)
10.1016/j.cellsig.2010.03.016
Non-canonical functions of RGS proteins.
Nan Sethakorn (2010)
10.1016/S1877-1173(09)86003-1
Regulators of G protein signaling proteins as central components of G protein-coupled receptor signaling complexes.
K. Mccoy (2009)
10.1074/jbc.M109.049684
Endothelial and Smooth Muscle-derived Neuropilin-like Protein Regulates Platelet-derived Growth Factor Signaling in Human Vascular Smooth Muscle Cells by Modulating Receptor Ubiquitination*
X. Guo (2009)
10.1016/j.bbalip.2008.05.004
Sphingosine 1-phosphate, lysophosphatidic acid and growth factor signaling and termination.
N. Pyne (2008)
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