Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Granzyme-like Sequences In Bony Fish Shed Light On The Emergence Of Hematopoietic Serine Proteases During Vertebrate Evolution.

S. Wernersson, J. Reimer, M. Poorafshar, U. Karlson, N. Wermenstam, E. Bengtén, M. Wilson, L. Pilström, L. Hellman
Published 2006 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Hematopoietic serine proteases (SPs) are stored in the granules of different leukocytes and these enzymes are important effector molecules in the immune system of mammals. However, very little is known about the presence of these proteins in lower vertebrates. Herein, the primary structures of five novel fish SPs, from the Atlantic cod (Gadus morhua) and the channel catfish (Ictalurus punctatus), are presented. One of the cod SPs is a homologue to human GzmA and K. The other fish SPs identified are termed 'Gzm-like' and are distantly related to a large heterogeneous group of hematopoietic SPs, including most of the T-cell Gzms (B-H), the mast cell chymases, the mast cell/basophil proteases of the mouse mast cell protease-8 subfamily (M8-family) and the neutrophil cathepsin G. Extensive BLAST-searches in genome and expressed sequence tag (EST) databases identified 40 additional teleost SPs related to the mammalian hematopoietic SP family. Subsequent phylogenetical analyses clearly demonstrate that the diversification into different subgroups within the GzmB/chymase/cathepsin G-related family has occurred independently in bony fishes and in mammals. In contrast, our findings suggest that the three subgroups, including (1) GzmK and the potent apoptosis-inducing GzmA, (2) the neutrophil proteases (proteinase 3, N-elastase and azurocidin), and (3) adipsin, have all evolved as distinct groups before the separation of tetrapods from the ray-finned fish approximately 420 million years ago.
This paper references
Generation of Catalytically Active Granzyme K fromEscherichia coli Inclusion Bodies and Identification of Efficient Granzyme K Inhibitors in Human Plasma*
E. Wilharm (1999)
Granzymes are the essential downstream effector molecules for the control of primary virus infections by cytolytic leukocytes.
A. Müllbacher (1999)
Expression of recombinant human granzyme B. A processing and activation role for dipeptidyl peptidase I.
M. Smyth (1995)
Ontogeny of lymphoid organs and immunoglobulin producing cells in Atlantic cod (Gadus morhua L.).
M. B. Schrøder (1998)
A despecialization step underlying evolution of a family of serine proteases.
M. A. Wouters (2003)
T-cell antigen receptors in Atlantic cod (Gadus morhua L.): structure, organisation and expression of TCR α and β genes
N. Wermenstam (2001)
The Restricted Expression of Granzyme M in Human Lymphocytes1
T. Sayers (2001)
Molecular evolution of the vertebrate blood coagulation network.
C. J. Davidson (2003)
Identification of granzyme A isolated from cytotoxic T‐lymphocyte‐granules as one of the proteases encoded by CTL‐specific genes
D. Masson (1986)
Channel catfish cytotoxic cells: a mini-review.
L. Shen (2002)
The versatility of proteolytic enzymes
H. Neurath (1986)
The Human Cytotoxic T Cell Granule Serine Protease Granzyme H Has Chymotrypsin-like (Chymase) Activity and Is Taken Up into Cytoplasmic Vesicles Reminiscent of Granzyme B-containing Endosomes*
K. Edwards (1999)
Identification and characterization of clonal NK-like cells from channel catfish (Ictalurus punctatus).
L. Shen (2004)
Characterisation of echidna IgM provides insights into the time of divergence of extant mammals.
K. Belov (2002)
Rat Mast Cell Protease 4 Is a β-Chymase with Unusually Stringent Substrate Recognition Profile*
U. Karlson (2002)
Bioinformatics of granzymes: sequence comparison and structural studies on granzyme family by homology modeling.
R. Sattar (2003)
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
J. Thompson (1997)
A primitive sarcopterygian fish with an eyestalk
M. Zhu (2001)
Cloning of IgE from the echidna (Tachyglossus aculeatus) and a comparative analysis of epsilon chains from all three extant mammalian lineages.
M. Vernersson (2004)
Extended Substrate Specificity of Rat Mast Cell Protease 5, a Rodent α-Chymase with Elastase-like Primary Specificity*
U. Karlson (2003)
Heterogeneity of Channel Catfish CTL with Respect to Target Recognition and Cytotoxic Mechanisms Employed1
H. Zhou (2001)
Characterization of cDNA clones encoding mouse proteinase 3 (myeloblastine) and cathepsin G
M. Aveskogh (1997)
Evidence for the Existence of Granzyme-Like Serine Proteases in Teleost Cytotoxic Cells
K. Praveen (2003)
Abnormal mast cells in mice deficient in a heparin-synthesizing enzyme
E. Forsberg (1999)
Secretory Granule Proteases in Rat Mast Cells. Cloning of 10 Different Serine Proteases and a Carboxypeptidase A from Various Rat Mast Cell Populations
C. Lützelschwab (1997)
Structural basis of substrate specificity in the serine proteases
J. Perona (1995)
Improved prediction of signal peptides: SignalP 3.0.
J. D. Bendtsen (2004)
Granzymes: a family of lymphocyte granule serine proteases
J. Trapani (2001)
Dipeptidyl peptidase I is required for the processing and activation of granzymes A and B in vivo.
C. Pham (1999)
A pore-forming protein, perforin, from a non-mammalian organism, Japanese flounder, Paralichthys olivaceus
J. Y. Hwang (2004)
Comparative modeling methods: Application to the family of the mammalian serine proteases
J. Greer (1990)
Granzyme A is critical for recovery of mice from infection with the natural cytopathic viral pathogen, ectromelia.
A. Müllbacher (1996)
Development and Analysis of Various Clonal Alloantigen- Dependent Cytotoxic Cell Lines from Channel Catfish1 2
T. Stuge (2000)
Identification and structural analysis of four serine proteases in a monotreme, the platypus, Ornithorhynchus anatinus
M. Poorafshar (2000)
Mouse Chromosome 17A3.3 Contains 13 Genes That Encode Functional Tryptic-like Serine Proteases with Distinct Tissue and Cell Expression Patterns*
G. W. Wong (2004)
Cell death and immunity: The ABCs of granule-mediated cytotoxicity: new weapons in the arsenal
J. Lieberman (2003)
The orphan granzymes of humans and mice.
W. Grossman (2003)
Development of leukocyte cell lines from the channel catfish (Ictalurus punctatus)
N. Miller (1994)
Identification and characterization of a FasL-like protein and cDNAs encoding the channel catfish death-inducing signaling complex
S. Long (2004)
Expression of Human Recombinant Granzyme A Zymogen and Its Activation by the Cysteine Proteinase Cathepsin C (*)
J. A. Kummer (1996)
Genes for mast-cell serine protease and their molecular evolution
R. Huang (2004)

This paper is referenced by
High degree of conservation of the multigene tryptase locus over the past 150–200 million years of mammalian evolution
J. Reimer (2010)
Granule proteases of hematopoietic cells, a family of versatile inflammatory mediators - an update on their cleavage specificity, in vivo substrates, and evolution.
L. Hellman (2014)
Developmental and Comparative Immunology
Yuta Matsuura (2014)
Fish Granzyme A Shows a Greater Role Than Granzyme B in Fish Innate Cell-Mediated Cytotoxicity
E. Chaves-Pozo (2019)
The Phylogenetic Profi le of Mast Cells
Luciana Travan (2018)
Asp-ase Activity of the Opossum Granzyme B Supports the Role of Granzyme B as Part of Anti-Viral Immunity Already during Early Mammalian Evolution
Zhirong Fu (2016)
Analysis of the evolution of granule associated serine proteases of immune defence (GASPIDs) suggests a revised nomenclature
Jamshaid Ahmad (2014)
Neutrophil Development, Migration, and Function in Teleost Fish
J. J. Havixbeck (2015)
The progress and promise of zebrafish as a model to study mast cells.
Sergey V Prykhozhij (2014)
Probiotics-pathogen interactions elicit differential regulation of cutaneous immune responses in epidermal cells of Atlantic cod Gadus morhua.
C. Lazado (2014)
Mast cell peptidases: chameleons of innate immunity and host defense.
N. Trivedi (2010)
Health and Environment in Aquaculture
E. D. Carvalho (2012)
Channel catfish granzyme-like I is a highly specific serine protease with metase activity that is expressed by fish NK-like cells.
M. Thorpe (2016)
Profiling gene expression in the spleen of Atlantic cod, Gadus morhua upon vaccination with Vibrio anguillarum antigen.
C. M. Caipang (2009)
Protease signaling in animal and plant‐regulated cell death
G. Salvesen (2016)
Expansion of the mast cell chymase locus over the past 200 million years of mammalian evolution
Maike Gallwitz (2006)
A novel image analysis approach to characterise the effects of dietary components on intestinal morphology and immune system in Atlantic salmon
Polyana Mayume Pereira da Silva (2013)
Teleost cytotoxic T cells.
T. Yamaguchi (2019)
Identification and characterization of common carp (Cyprinus carpio L.) granzyme A/K, a cytotoxic cell granule-associated serine protease.
R. Huang (2010)
The mast cell: an evolutionary perspective
E. Crivellato (2010)
Expression profiles of genes associated with immune response and oxidative stress in Atlantic cod, Gadus morhua head kidney leukocytes modulated by live and heat-inactivated intestinal bacteria.
C. Lazado (2010)
Identification of a novel fish granzyme involved in cell-mediated immunity.
Yuta Matsuura (2014)
The salmonid cathelicidins: a gene family with highly varied C-terminal antimicrobial domains.
M. Scocchi (2009)
What Is the Evolutionary Fingerprint in Neutrophil Granulocytes?
Leonie Fingerhut (2020)
Temperature-dependent viral replication and antiviral apoptotic response in viral haemorrhagic septicaemia virus (VHSV)-infected olive flounder (Paralichthys olivaceus).
S. Avunje (2012)
Response of gut microbiota and immune function to hypoosmotic stress in the yellowfin seabream (Acanthopagrus latus).
Genmei Lin (2020)
Correction: Granule Associated Serine Proteases of Hematopoietic Cells – An Analysis of Their Appearance and Diversification during Vertebrate Evolution
Srinivas Akula (2015)
Haematopoietic Serine Proteases : A Cleavage Specificity Analysis
Michael Thorpe (2014)
Chemoattractants, extracellular proteases, and the integrated host defense response.
B. Zabel (2006)
Fish T cells: recent advances through genomics.
K. Laing (2011)
Mast cell proteases.
G. Pejler (2007)
GZMA (granzyme A (granzyme 1, cytotoxic T - lymphocyte -associated serine esterase 3))
E. Catalán (2012)
See more
Semantic Scholar Logo Some data provided by SemanticScholar