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Electrochemiluminescence Acetylcholine Biosensor Based On Biofunctional AMs-AChE-ChO Biocomposite And Electrodeposited Graphene-Au-chitosan Nanocomposite

X. Wu, X. Zhong, Y. Chai, R. Yuan
Published 2014 · Chemistry

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Abstract A novel electrochemiluminescence (ECL) biosensing system was designed for the detection of acetylthiocholine chloride (ATCl) in this work. First, graphene-AuNPs-chitosan (GR-AuNPs-CS) nanocomposite, which possesses the property of intensification effect on the ECL of luminol, was electrochemically deposited on the bare GCE. Then, a biofunctional Fe 3 O 4 -TiO 2 -AChE-ChO biocomposite was unprecedentedly prepared which exhibited the mimic peroxidase activity of Fe 3 O 4 and the enhancement effect of TiO 2 on the ECL intensity of luminol. Subsequently, the substrate ATCl was hydrolyzed by AChE of AChE-ChO multiple enzymes to generate thiocholine, which was then catalyzed by ChO to produce H 2 O 2 in situ . H 2 O 2 , as the coreactant of luminol-ECL system can enhance the ECL intensity of luminol. On the basis of this principle and smart integration of the above nanocomposites under the optimal conditions, the resulting ECL biosensor for ATCl showed wide linear range from 6.7 nM to 0.92 mM with detection limit (signal-to-noise ratio of 3) of 2.2 nM. The excellent performance of the biosensor makes this biosensing system suitable and promising in the practical application of choline type of biomass.
This paper references
10.1021/ac200237j
Positive potential operation of a cathodic electrogenerated chemiluminescence immunosensor based on luminol and graphene for cancer biomarker detection.
Shoujiang Xu (2011)
10.1021/ac900136z
Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide.
M. Zhou (2009)
10.1039/c2an16268g
Intensification of electrochemiluminescence of luminol on TiO2 supported Au atomic cluster nano-hybrid modified electrode.
Zhimin Yu (2012)
10.1016/J.SNB.2013.09.049
Graphene/polyaniline/gold nanoparticles nanocomposite for the direct electron transfer of glucose oxidase and glucose biosensing
Q. Xu (2014)
10.1016/J.ELECTACTA.2012.11.037
Electrodeposition for preparation of efficient surface-enhanced Raman scattering-active silver nanoparticle substrates for neurotransmitter detection
Marta Siek (2013)
10.1016/j.colsurfb.2011.02.003
Preparation of surface plasmon resonance biosensor based on magnetic core/shell Fe3O4/SiO2 and Fe3O4/Ag/SiO2 nanoparticles.
L. Wang (2011)
10.1002/ELAN.200804580
Study on Sensitization from Reactive Oxygen Species for Electrochemiluminescence of Luminol in Neutral Medium
H. Chu (2009)
10.1021/BM034130M
Chitosan as antimicrobial agent: applications and mode of action.
E. Rabea (2003)
10.1007/S11426-011-4357-0
The intensification of luminol electrochemiluminescence by metallic oxide nanoparticles
W. Guo (2011)
10.1016/J.CARBON.2012.10.037
Synthesis of Pt nanoparticles anchored on graphene-encapsulated Fe3O4 magnetic nanospheres and their use as catalysts for methanol oxidation
Shaoxiong Lin (2013)
10.1021/CR0500535
Titanium dioxide nanomaterials: synthesis, properties, modifications, and applications.
X. Chen (2007)
10.1016/J.JELECHEM.2013.05.009
A nano-TiO2 supported AuAg alloy nanocluster functionalized electrode for sensitizing the electrochemiluminescent analysis
Xiuhua Wei (2013)
10.1016/J.SNB.2013.11.092
Improved enzyme immobilization for enhanced bioelectrocatalytic activity of choline sensor
Y. T. Wang (2013)
10.1021/ac300867s
Enzyme mimics of Au/Ag nanoparticles for fluorescent detection of acetylcholine.
Chen-I Wang (2012)
10.1016/S0731-7085(02)00674-X
Detection of choline and acetylcholine in a pharmaceutical preparation using high-performance liquid chromatography/electrospray ionization mass spectrometry.
R. Dunphy (2003)
10.1016/j.bioelechem.2014.02.003
Sensitive bi-enzymatic biosensor based on polyphenoloxidases-gold nanoparticles-chitosan hybrid film-graphene doped carbon paste electrode for carbamates detection.
T. M. Oliveira (2014)
10.1007/s00604-012-0895-1
Biocentri-voltammetric biosensor for acetylcholine and choline
S. Çevik (2012)
10.1021/AC049889I
Multichannel electrochemiluminescence of luminol in neutral and alkaline aqueous solutions on a gold nanoparticle self-assembled electrode.
Hua Cui (2004)
10.1038/nnano.2007.260
Intrinsic peroxidase-like activity of ferromagnetic nanoparticles.
Lizeng Gao (2007)
10.1186/1471-2202-15-14
ROS-mediated activation of Drosophila larval nociceptor neurons by UVC irradiation
Myung-jun Kim (2013)
10.1021/JA0379215
Probing photoelectrochemical processes in Au-CdS nanoparticle arrays by surface plasmon resonance: application for the detection of acetylcholine esterase inhibitors.
M. Zayats (2003)
10.1021/la703010z
Acetylcholine detection at micromolar concentrations with the use of an artificial receptor-based fluorescence switch.
N. Korbakov (2008)
10.1016/j.bios.2013.09.006
An eco-friendly, simple, and sensitive fluorescence biosensor for the detection of choline and acetylcholine based on C-dots and the Fenton reaction.
J. Wei (2014)
10.1002/ADFM.200801313
Signal‐On Electrochemiluminescence Biosensors Based on CdS–Carbon Nanotube Nanocomposite for the Sensitive Detection of Choline and Acetylcholine
X. Wang (2009)
10.1016/J.ELECTACTA.2012.06.051
Non-enzymatic acetylcholine sensor based on Ni–Al layered double hydroxides/ordered mesoporous carbon
J. Ju (2012)
10.1016/J.TRAC.2008.05.008
Electrochemical analysis with nanoparticle-based biosystems
A. Escosura-Muñiz (2008)
10.1016/J.SNB.2014.02.026
A novel surface modification strategy of CdTe/CdS QDs and its application for sensitive detection of ct-DNA
Wanjun Liang (2014)
10.1016/J.ELECTACTA.2014.07.008
A novel dual-template molecularly imprinted electrochemiluminescence immunosensor array using Ru(bpy)32+-Silica@Poly-L-lysine-Au composite nanoparticles as labels for near-simultaneous detection of tumor markers
Feng Xiao-bin (2014)
10.1016/j.aca.2011.06.014
An amperometric biosensor based on acetylcholinesterase immobilized onto iron oxide nanoparticles/multi-walled carbon nanotubes modified gold electrode for measurement of organophosphorus insecticides.
N. Chauhan (2011)
10.1016/J.ELECTACTA.2013.12.044
Novel Graphene-Gold Hybrid Nanostructures Constructed via Sulfur Modified Graphene: Preparation and Characterization by Surface and Electrochemical Techniques
R. K. Shervedani (2014)
10.1016/J.ELECTACTA.2014.01.074
A novel and sensitive electrochemical DNA biosensor based on Fe@Au nanoparticles decorated graphene oxide
M. L. Yola (2014)
10.1002/ELAN.200603584
Electrochemiluminescent determination of chlorphenamine maleate based on ru(bpy) immobilized in a nano-titania/nafion membrane
Hongjie Song (2006)
10.1021/cr300115g
Graphene oxide: preparation, functionalization, and electrochemical applications.
Da Chen (2012)
10.1016/J.ELECTACTA.2014.02.103
Synthesis of multiwall carbon nanotubes-graphene oxide-thionine-Au nanocomposites for electrochemiluminescence detection of cholesterol
X. Wu (2014)
10.1016/j.bios.2013.08.043
Direct electron transfer of glucose oxidase and biosensing for glucose based on PDDA-capped gold nanoparticle modified graphene/multi-walled carbon nanotubes electrode.
Y. Yu (2014)



This paper is referenced by
10.1002/9783527807918.CH7
Recent Advances in Chitosan‐Based Films for Novel Biosensor
Akil Ahmad (2018)
10.3390/APP9040797
Biosensor Applications of Electrodeposited Nanostructures
K. Stine (2019)
10.3762/bjnano.9.111
Electrodeposition of reduced graphene oxide with chitosan based on the coordination deposition method
M. Liu (2018)
10.1016/J.ELECTACTA.2018.10.084
A solid-state electrochemiluminescence biosensor for Con A detection based on CeO2@Ag nanoparticles modified graphene quantum dots as signal probe
Fumei Zuo (2019)
10.1016/J.ELECTACTA.2018.03.056
Electrogenerated chemiluminescence from the monomer of a tetradentate chelate Pt(II) compound
Shuijian He (2018)
10.1002/ELAN.201500339
Electrochemical Biosensor for Organophosphate Pesticides and Huperzine‐A Detection Based on Pd Wormlike Nanochains/Graphitic Carbon Nitride Nanocomposites and Acetylcholinesterase
Bing-xin Wang (2016)
10.1016/j.molstruc.2019.127039
Enzyme immobilization on polymeric microspheres containing Schiff base for detection of organophosphate and carbamate insecticides
Dilek Nartop (2020)
10.1016/J.SNB.2018.09.051
Determination of carbamate pesticide in food using a biosensor based on reduced graphene oxide and acetylcholinesterase enzyme
Martin K L Silva (2018)
10.1016/j.talanta.2016.05.030
Electrochemiluminescence biosensor for determination of organophosphorous pesticides based on bimetallic Pt-Au/multi-walled carbon nanotubes modified electrode.
S. Miao (2016)
10.1080/00032719.2017.1291654
Selective Electrochemical Determination of Salicylic Acid in Wheat Using Molecular Imprinted Polymers
L. Ma (2017)
10.1016/j.msec.2017.05.098
Novel biomimetic composite material for potentiometric screening of acetylcholine, a neurotransmitter in Alzheimer's disease.
A. S. Sacramento (2017)
10.1016/J.ELECTACTA.2016.07.149
An electrochemiluminescence immunosensor based on ABEI and FCA functionalized Platinum@Copper hierarchical trigonal bipyramid nanoframes
Huiyun Yang (2016)
10.1016/j.bios.2017.11.016
A molecularly imprinted electrochemiluminescence sensor based on upconversion nanoparticles enhanced by electrodeposited rGO for selective and ultrasensitive detection of clenbuterol.
X. Jin (2018)
10.1016/j.ijbiomac.2019.02.025
Box-Behnken design to optimize the synthesis of new crosslinked chitosan-glyoxal/TiO2 nanocomposite: Methyl orange adsorption and mechanism studies.
A. Mohammad (2019)
10.1016/J.BEJ.2017.10.008
Acetylcholinesterase/chitosan-transition metal carbides nanocomposites-based biosensor for the organophosphate pesticides detection
Liya Zhou (2017)
10.1016/J.MATERRESBULL.2017.09.068
Structural characterization of chitosan-smectite nanocomposite and its application in the development of a novel potentiometric monohydrogen phosphate-selective sensor
Cihan Topcu (2018)
10.1016/j.jelechem.2020.113883
Acetylcholinesterase-choline oxidase-based mini-reactors coupled with silver solid amalgam electrode for amperometric detection of acetylcholine in flow injection analysis
Sofiia Tvorynska (2020)
10.1007/s00216-018-1513-9
An ultrasensitive signal-on electrochemiluminescence biosensor based on Au nanoclusters for detecting acetylthiocholine
Cong Zhang (2018)
10.1039/C9NJ02556A
The development of a novel biosensor based on gold nanocages/graphene oxide–chitosan modified acetylcholinesterase for organophosphorus pesticide detection
Y. Yao (2019)
10.4155/bio-2016-0178
A review of electrogenerated chemiluminescent biosensors for assays in biological matrices.
E. Gross (2016)
10.1002/ELAN.201700845
Mesoporous Bimetallic PtPd Nanoflowers as a Platform to Enhance Electrocatalytic Activity of Acetylcholinesterase for Organophosphate Pesticide Detection
L. Ma (2018)
10.1016/j.jpha.2018.11.004
Recent advances in electrogenerated chemiluminescence biosensing methods for pharmaceuticals
Y. Zhang (2019)
10.1016/j.aca.2015.06.004
Fenugreek hydrogel-agarose composite entrapped gold nanoparticles for acetylcholinesterase based biosensor for carbamates detection.
R. M. Kestwal (2015)
10.1016/J.CEJ.2018.01.111
Silver nanoparticles modified two-dimensional transition metal carbides as nanocarriers to fabricate acetycholinesterase-based electrochemical biosensor
Yanjun Jiang (2018)
10.5004/dwt.2019.24384
Modeling and mechanism of reactive orange 16 dye adsorption by chitosan-glyoxal/TiO2 nanocomposite: application of response surface methodology
A. Abdulhameed (2019)
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