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Electrochemiluminescence Acetylcholine Biosensor Based On Biofunctional AMs-AChE-ChO Biocomposite And Electrodeposited Graphene-Au-chitosan Nanocomposite
X. Wu, X. Zhong, Y. Chai, R. Yuan
Published 2014 · Chemistry
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Abstract A novel electrochemiluminescence (ECL) biosensing system was designed for the detection of acetylthiocholine chloride (ATCl) in this work. First, graphene-AuNPs-chitosan (GR-AuNPs-CS) nanocomposite, which possesses the property of intensification effect on the ECL of luminol, was electrochemically deposited on the bare GCE. Then, a biofunctional Fe 3 O 4 -TiO 2 -AChE-ChO biocomposite was unprecedentedly prepared which exhibited the mimic peroxidase activity of Fe 3 O 4 and the enhancement effect of TiO 2 on the ECL intensity of luminol. Subsequently, the substrate ATCl was hydrolyzed by AChE of AChE-ChO multiple enzymes to generate thiocholine, which was then catalyzed by ChO to produce H 2 O 2 in situ . H 2 O 2 , as the coreactant of luminol-ECL system can enhance the ECL intensity of luminol. On the basis of this principle and smart integration of the above nanocomposites under the optimal conditions, the resulting ECL biosensor for ATCl showed wide linear range from 6.7 nM to 0.92 mM with detection limit (signal-to-noise ratio of 3) of 2.2 nM. The excellent performance of the biosensor makes this biosensing system suitable and promising in the practical application of choline type of biomass.
This paper references
Positive potential operation of a cathodic electrogenerated chemiluminescence immunosensor based on luminol and graphene for cancer biomarker detection.
Shoujiang Xu (2011)
Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide.
M. Zhou (2009)
Intensification of electrochemiluminescence of luminol on TiO2 supported Au atomic cluster nano-hybrid modified electrode.
Zhimin Yu (2012)
Graphene/polyaniline/gold nanoparticles nanocomposite for the direct electron transfer of glucose oxidase and glucose biosensing
Q. Xu (2014)
Electrodeposition for preparation of efficient surface-enhanced Raman scattering-active silver nanoparticle substrates for neurotransmitter detection
Marta Siek (2013)
Preparation of surface plasmon resonance biosensor based on magnetic core/shell Fe3O4/SiO2 and Fe3O4/Ag/SiO2 nanoparticles.
L. Wang (2011)
Study on Sensitization from Reactive Oxygen Species for Electrochemiluminescence of Luminol in Neutral Medium
H. Chu (2009)
Chitosan as antimicrobial agent: applications and mode of action.
E. Rabea (2003)
The intensification of luminol electrochemiluminescence by metallic oxide nanoparticles
W. Guo (2011)
Synthesis of Pt nanoparticles anchored on graphene-encapsulated Fe3O4 magnetic nanospheres and their use as catalysts for methanol oxidation
Shaoxiong Lin (2013)
Titanium dioxide nanomaterials: synthesis, properties, modifications, and applications.
X. Chen (2007)
A nano-TiO2 supported AuAg alloy nanocluster functionalized electrode for sensitizing the electrochemiluminescent analysis
Xiuhua Wei (2013)
Improved enzyme immobilization for enhanced bioelectrocatalytic activity of choline sensor
Y. T. Wang (2013)
Enzyme mimics of Au/Ag nanoparticles for fluorescent detection of acetylcholine.
Chen-I Wang (2012)
Detection of choline and acetylcholine in a pharmaceutical preparation using high-performance liquid chromatography/electrospray ionization mass spectrometry.
R. Dunphy (2003)
Sensitive bi-enzymatic biosensor based on polyphenoloxidases-gold nanoparticles-chitosan hybrid film-graphene doped carbon paste electrode for carbamates detection.
T. M. Oliveira (2014)
Biocentri-voltammetric biosensor for acetylcholine and choline
S. Çevik (2012)
Multichannel electrochemiluminescence of luminol in neutral and alkaline aqueous solutions on a gold nanoparticle self-assembled electrode.
Hua Cui (2004)
Intrinsic peroxidase-like activity of ferromagnetic nanoparticles.
Lizeng Gao (2007)
ROS-mediated activation of Drosophila larval nociceptor neurons by UVC irradiation
Myung-jun Kim (2013)
Probing photoelectrochemical processes in Au-CdS nanoparticle arrays by surface plasmon resonance: application for the detection of acetylcholine esterase inhibitors.
M. Zayats (2003)
Acetylcholine detection at micromolar concentrations with the use of an artificial receptor-based fluorescence switch.
N. Korbakov (2008)
An eco-friendly, simple, and sensitive fluorescence biosensor for the detection of choline and acetylcholine based on C-dots and the Fenton reaction.
J. Wei (2014)
Signal‐On Electrochemiluminescence Biosensors Based on CdS–Carbon Nanotube Nanocomposite for the Sensitive Detection of Choline and Acetylcholine
X. Wang (2009)
Non-enzymatic acetylcholine sensor based on Ni–Al layered double hydroxides/ordered mesoporous carbon
J. Ju (2012)
Electrochemical analysis with nanoparticle-based biosystems
A. Escosura-Muñiz (2008)
A novel surface modification strategy of CdTe/CdS QDs and its application for sensitive detection of ct-DNA
Wanjun Liang (2014)
A novel dual-template molecularly imprinted electrochemiluminescence immunosensor array using Ru(bpy)32+-Silica@Poly-L-lysine-Au composite nanoparticles as labels for near-simultaneous detection of tumor markers
Feng Xiao-bin (2014)
An amperometric biosensor based on acetylcholinesterase immobilized onto iron oxide nanoparticles/multi-walled carbon nanotubes modified gold electrode for measurement of organophosphorus insecticides.
N. Chauhan (2011)
Novel Graphene-Gold Hybrid Nanostructures Constructed via Sulfur Modified Graphene: Preparation and Characterization by Surface and Electrochemical Techniques
R. K. Shervedani (2014)
A novel and sensitive electrochemical DNA biosensor based on Fe@Au nanoparticles decorated graphene oxide
M. L. Yola (2014)
Electrochemiluminescent determination of chlorphenamine maleate based on ru(bpy) immobilized in a nano-titania/nafion membrane
Hongjie Song (2006)
Graphene oxide: preparation, functionalization, and electrochemical applications.
Da Chen (2012)
Synthesis of multiwall carbon nanotubes-graphene oxide-thionine-Au nanocomposites for electrochemiluminescence detection of cholesterol
X. Wu (2014)
Direct electron transfer of glucose oxidase and biosensing for glucose based on PDDA-capped gold nanoparticle modified graphene/multi-walled carbon nanotubes electrode.
Y. Yu (2014)
This paper is referenced by
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Biosensor Applications of Electrodeposited Nanostructures
K. Stine (2019)
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M. Liu (2018)
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Fumei Zuo (2019)
Electrogenerated chemiluminescence from the monomer of a tetradentate chelate Pt(II) compound
Shuijian He (2018)
Electrochemical Biosensor for Organophosphate Pesticides and Huperzine‐A Detection Based on Pd Wormlike Nanochains/Graphitic Carbon Nitride Nanocomposites and Acetylcholinesterase
Bing-xin Wang (2016)
Enzyme immobilization on polymeric microspheres containing Schiff base for detection of organophosphate and carbamate insecticides
Dilek Nartop (2020)
Determination of carbamate pesticide in food using a biosensor based on reduced graphene oxide and acetylcholinesterase enzyme
Martin K L Silva (2018)
Electrochemiluminescence biosensor for determination of organophosphorous pesticides based on bimetallic Pt-Au/multi-walled carbon nanotubes modified electrode.
S. Miao (2016)
Selective Electrochemical Determination of Salicylic Acid in Wheat Using Molecular Imprinted Polymers
L. Ma (2017)
Novel biomimetic composite material for potentiometric screening of acetylcholine, a neurotransmitter in Alzheimer's disease.
A. S. Sacramento (2017)
An electrochemiluminescence immunosensor based on ABEI and FCA functionalized Platinum@Copper hierarchical trigonal bipyramid nanoframes
Huiyun Yang (2016)
A molecularly imprinted electrochemiluminescence sensor based on upconversion nanoparticles enhanced by electrodeposited rGO for selective and ultrasensitive detection of clenbuterol.
X. Jin (2018)
Box-Behnken design to optimize the synthesis of new crosslinked chitosan-glyoxal/TiO2 nanocomposite: Methyl orange adsorption and mechanism studies.
A. Mohammad (2019)
Acetylcholinesterase/chitosan-transition metal carbides nanocomposites-based biosensor for the organophosphate pesticides detection
Liya Zhou (2017)
Structural characterization of chitosan-smectite nanocomposite and its application in the development of a novel potentiometric monohydrogen phosphate-selective sensor
Cihan Topcu (2018)
Acetylcholinesterase-choline oxidase-based mini-reactors coupled with silver solid amalgam electrode for amperometric detection of acetylcholine in flow injection analysis
Sofiia Tvorynska (2020)
An ultrasensitive signal-on electrochemiluminescence biosensor based on Au nanoclusters for detecting acetylthiocholine
Cong Zhang (2018)
The development of a novel biosensor based on gold nanocages/graphene oxide–chitosan modified acetylcholinesterase for organophosphorus pesticide detection
Y. Yao (2019)
A review of electrogenerated chemiluminescent biosensors for assays in biological matrices.
E. Gross (2016)
Mesoporous Bimetallic PtPd Nanoflowers as a Platform to Enhance Electrocatalytic Activity of Acetylcholinesterase for Organophosphate Pesticide Detection
L. Ma (2018)
Recent advances in electrogenerated chemiluminescence biosensing methods for pharmaceuticals
Y. Zhang (2019)
Fenugreek hydrogel-agarose composite entrapped gold nanoparticles for acetylcholinesterase based biosensor for carbamates detection.
R. M. Kestwal (2015)
Silver nanoparticles modified two-dimensional transition metal carbides as nanocarriers to fabricate acetycholinesterase-based electrochemical biosensor
Yanjun Jiang (2018)
Modeling and mechanism of reactive orange 16 dye adsorption by chitosan-glyoxal/TiO2 nanocomposite: application of response surface methodology
A. Abdulhameed (2019)