Online citations, reference lists, and bibliographies.
← Back to Search

Chromosomal High Mobility Group (HMG) Proteins Of The HMGB-type Occurring In The Moss Physcomitrella Patens.

Bruno Kiilerich, C. Stemmer, T. Merkle, D. Launholt, G. Gorr, K. Grasser
Published 2008 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
High mobility group (HMG) proteins of the HMGB family are chromatin-associated proteins that act as architectural factors in nucleoprotein structures, which regulate DNA-dependent processes including transcription. Members of the HMGB family have been characterised from various mono-and dicot plants, but not from lower plant species. Here, we have identified three candidate HMGB proteins encoded in the genome of the moss Physcomitrella patens. The structurally similar HMGB2 and HMGB3 proteins display the typical overall structure of higher plant HMGB proteins consisting of a central HMG-box DNA-binding domain that is flanked by a basic N-terminal and an acidic C-terminal domain. The HMGB1 protein differs from higher plant HMGB proteins by having a very extensive N-terminal domain and by lacking the acidic C-terminal domain. Like higher plant HMGB proteins, HMGB3 localises to the cell nucleus, but HMGB1 is targeted to plastids. Analysis of the HMG-box domains of HMGB1 and HMGB3 by CD revealed that HMGB1box and the HMGB3box have an alpha-helical structure. While the HMGB3box interacts with DNA comparable to typical higher plant counterparts, the HMGB1box has only a low affinity for DNA. Cotransformation assays in Physcomitrella protoplasts demonstrated that expression of HMGB3 resulted in repression of reporter gene expression. In summary, our data show that functional HMGB-type proteins occur in Physcomitrella and most likely in other lower plant species.
This paper references
High mobility group proteins 1 and 2 function as general class II transcription factors.
J. Singh (1990)
Arabidopsis Chromatin-Associated HMGA and HMGB Use Different Nuclear Targeting Signals and Display Highly Dynamic Localization within the Nucleus[W]
D. Launholt (2006)
HMG1 and 2, and related 'architectural' DNA-binding proteins.
J. Thomas (2001)
HMGB proteins and gene expression.
A. Agresti (2003)
HMG-1 enhances HMG-I/Y binding to an A/T-rich enhancer element from the pea plastocyanin gene.
C. I. Webster (2001)
High-mobility group chromosomal proteins of wheat.
S. Spiker (1984)
Interaction of wheat high-mobility-group proteins with four-way-junction DNA and characterization of the structure and expression of HMGA gene.
W. Zhang (2003)
Regulation of Transcription in Plants
K. Grasser (2007)
Characterization of transgenic Arabidopsis plants overexpressing high mobility group B proteins under high salinity, drought or cold stress.
K. J. Kwak (2007)
High-Mobility Group Chromatin Proteins 1 and 2 Functionally Interact with Steroid Hormone Receptors To Enhance Their DNA Binding In Vitro and Transcriptional Activity in Mammalian Cells
V. Boonyaratanakornkit (1998)
High-mobility-group chromosomal proteins: architectural components that facilitate chromatin function.
M. Bustin (1996)
In vivo glyco‐engineered antibody with improved lytic potential produced by an innovative non‐mammalian expression system
M. Schuster (2007)
A Role of Basic Residues and the Putative Intercalating Phenylalanine of the HMG-1 Box B in DNA Supercoiling and Binding to Four-way DNA Junctions*
M. Štros (2000)
High mobility group proteins HMG-1 and HMG-I/Y bind to a positive regulatory region of the pea plastocyanin gene promoter.
C. I. Webster (1997)
HMG proteins: dynamic players in gene regulation and differentiation.
M. Bianchi (2005)
Specificity of the Stimulatory Interaction between Chromosomal HMGB Proteins and the Transcription Factor Dof2 and Its Negative Regulation by Protein Kinase CK2-mediated Phosphorylation*
N. M. Krohn (2002)
Abundance of mRNAs encoding HMG1/HMG2 class high-mobility-group DNA-binding proteins are differentially regulated in cotyledons of Pharbitis nil
S. O'neill (2004)
The role of intercalating residues in chromosomal high-mobility-group protein DNA binding, bending and specificity.
Janet Klass (2003)
The HMG-box: a versatile protein domain occurring in a wide variety of DNA-binding proteins
M. Štros (2007)
Stimulatory effect of the maize HMGa protein on reporter gene expression in maize protoplasts
K. Grasser (1993)
Regulation of DNA-Dependent Activities by the Functional Motifs of the High-Mobility-Group Chromosomal Proteins
M. Bustin (1999)
A set of plant expression vectors for transcriptional and translational fusions.
R. Töpfer (1987)
Nuclear export of proteins in plants: AtXPO1 is the export receptor for leucine-rich nuclear export signals in Arabidopsis thaliana.
D. Haasen (1999)
Differential chromatin association and nucleosome binding of the maize HMGA, HMGB, and SSRP1 proteins.
J. Lichota (2001)
High mobility group proteins of the plant HMGB family: dynamic chromatin modulators.
K. Grasser (2007)
Protein Kinase CK2 Differentially Phosphorylates Maize Chromosomal High Mobility Group B (HMGB) Proteins Modulating Their Stability and DNA Interactions*
Christian Stemmer (2002)
Chromatin‐associated Architectural HMGA and HMGB Proteins Assist Transcription Factor Function
K. Grasser (2007)
Rice HMGB1 protein recognizes DNA structures and bends DNA efficiently.
Q. Wu (2003)
Basic and acidic regions flanking the HMG domain of maize HMGa modulate the interactions with DNA and the self-association of the protein.
C. Ritt (1998)
Multifocal two-photon laser scanning microscopy combined with photo-activatable GFP for in vivo monitoring of intracellular protein dynamics in real time.
J. Martini (2007)
High-throughput protein localization in Arabidopsis using Agrobacterium-mediated transient expression of GFP-ORF fusions.
O. Koroleva (2005)
HMG1 protein stimulates DNA end joining by promoting association of DNA molecules via their ends.
M. Štros (2000)
High‐level expression of secreted complex glycosylated recombinant human erythropoietin in the Physcomitrella Δ‐fuc‐t Δ‐xyl‐t mutant
A. Weise (2007)
High mobility group protein-1 (HMG-1) is a unique activator of p53.
L. Jayaraman (1998)
High mobility group proteins 1 and 2 stimulate transcription in vitro by RNA polymerases II and III.
D. Tremethick (1986)
Intercalating residues determine the mode of HMG1 domains A and B binding to cisplatin-modified DNA.
Q. He (2000)
Occurrence of five different chromosomal HMG1 proteins in various maize tissues
Christian Stemmer (2004)
The Arabidopsis genome encodes structurally and functionally diverse HMGB-type proteins.
Marion Grasser (2006)
HMGB1 and HMGB2 Cell-specifically Down-regulate the p53- and p73-dependent Sequence-specific Transactivation from the Human Bax Gene Promoter*
M. Štros (2002)
Four differently chromatin-associated maize HMG domain proteins modulate DNA structure and act as architectural elements in nucleoprotein complexes.
C. Ritt (1998)
Maize Chromosomal HMGc
K. Grasser (1996)
Duplicated NHP6 genes of Saccharomyces cerevisiae encode proteins homologous to bovine high mobility group protein 1.
D. Kolodrubetz (1990)
Kinetic analysis of high-mobility-group proteins HMG-1 and HMG-I/Y binding to cholesterol-tagged DNA on a supported lipid monolayer.
C. I. Webster (2000)
Isolation and characterization of a cDNA encoding a high mobility group protein HMG-1 from Canavalia gladiata D.C.
S. Yamamoto (1998)
Chromatin‐mediated transcriptional regulation by the yeast architectural factors NHP6A and NHP6B
J. M. Moreira (2000)
Basic and acidic regions flanking the HMG-box domain of maize HMGB1 and HMGB5 modulate the stimulatory effect on the DNA binding of transcription factor Dof2.
Marion Grasser (2007)
HMG boxes of DSP1 protein interact with the rel homology domain of transcription factors.
M. Decoville (2000)
Revised nomenclature for high mobility group (HMG) chromosomal proteins.
M. Bustin (2001)
HMG1 interacts with HOX proteins and enhances their DNA binding and transcriptional activation.
V. Zappavigna (1996)
A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria.
J. F. Diffley (1991)
Binding to four‐way junction DNA: a common property of architectural proteins?
J. Zlatanova (1998)
Recognition of distorted DNA structures by HMG domains.
A. Travers (2000)
Variability in Arabidopsis thaliana chromosomal high-mobility-group-1-like proteins.
C. Stemmer (1997)
HMGB6 from Arabidopsis thaliana specifies a novel type of plant chromosomal HMGB protein.
K. Grasser (2004)
Histone H1 Enhances the DNA Binding Activity of the Transcription Factor EmBP-1*
T. Schultz (1996)
Overlapping expression patterns among the genes encoding Arabidopsis chromosomal high mobility group (HMG) proteins
D. Launholt (2007)
Chromatin-associated HMGA and HMGB proteins: versatile co-regulators of DNA-dependent processes
K. Grasser (2004)
Similarity of human mitochondrial transcription factor 1 to high mobility group proteins.
M. Parisi (1991)
High mobility group chromosomal proteins bind to AT-rich tracts flanking plant genes
T. J. Pedersen (2004)
Basis for recognition of cisplatin-modified DNA by high-mobility-group proteins
Uta-Maria Ohndorf (1999)
Optimisation of a bioreactor culture of the moss Physcomitrella patens for mass production of protoplasts
A. Hohe (2002)
High mobility group proteins 1 and 2 stimulate binding of a specific transcription factor to the adenovirus major late promoter.
F. Watt (1988)
Dof DNA-binding domains of plant transcription factors contribute to multiple protein-protein interactions.
S. Yanagisawa (1997)
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
J. Thompson (1997)
The structure of a chromosomal high mobility group protein–DNA complex reveals sequence‐neutral mechanisms important for non‐sequence‐specific DNA recognition
F. Murphy (1999)
Cloning and characterization of rice HMGB1 gene.
Q. Wu (2003)
The S. cerevisiae architectural HMGB protein NHP6A complexed with DNA: DNA and protein conformational changes upon binding.
J. E. Masse (2002)

This paper is referenced by
The Dengue Vector Aedes aegypti Contains a Functional High Mobility Group Box 1 (HMGB1) Protein with a Unique Regulatory C-Terminus
Fábio S Ribeiro (2012)
Transcript elongation factor TFIIS is involved in arabidopsis seed dormancy.
Marion Grasser (2009)
Plant Proteins Containing High Mobility Group Box DNA-Binding Domains Modulate Different Nuclear Processes1[W]
M. Antosch (2012)
The HMGB1-TLR4 axis contributes to myocardial ischemia/reperfusion injury via regulation of cardiomyocyte apoptosis.
Hua-sheng Ding (2013)
Update on Plant HMG-Box Proteins Plant Proteins Containing High Mobility Group Box DNA-Binding Domains Modulate Different Nuclear Processes 1 [ W ]
M. Antosch (2012)
High mobility group [corrected] box 1 mediates neutrophil recruitment in myocardial ischemia-reperfusion injury through toll like receptor 4-related pathway.
Hua-sheng Ding (2012)
Isolation and expression analysis of a candidate gametophyte sex determination gene (sjhmg) of kelp (Saccharina japonica)
L. Zhang (2019)
Physcomitrella HMGA-type proteins display structural differences compared to their higher plant counterparts.
Carina Lyngaard (2008)
Interference with HMGB1 increases the sensitivity to chemotherapy drugs by inhibiting HMGB1-mediated cell autophagy and inducing cell apoptosis
Ruiguang Zhang (2015)
Semantic Scholar Logo Some data provided by SemanticScholar