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Evaluation And Modification Of N-trimethyl Chitosan Chloride Nanoparticles As Protein Carriers.
Fu Chen, Zhi-Rong Zhang, Y. Huang
Published 2007 · Medicine, Chemistry
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N-Trimethyl chitosan chloride (TMC) nanoparticles were prepared by ionic crosslinking of TMC with tripolyphosphate (TPP). Two model proteins with different pI values, bovine serum albumin (BSA, pI=4.8) and bovine hemoglobin (BHb, pI=6.8), were used to investigate the loading and release features of the TMC nanoparticles. TMC samples with different degrees of quaternization were synthesized to evaluate its influence on the physicochemical properties and release profiles of the nanoparticles. Sodium alginate was used to modify the TMC nanoparticles to reduce burst release. The results indicated that the TMC nanoparticles had a high loading efficiency (95%) for BSA but a low one (30%) for BHb. The particle size and zeta potential were significantly affected by the BSA concentration but not by the BHb concentration. Nanoparticles of TMC with a lower degree of quaternization showed an increase in particle size, a decrease in zeta potential and a slower drug-release profile. As for the alginate-modified nanoparticles, a smaller size and lower zeta potential were observed and the burst release of BSA was reduced. These studies demonstrated that TMC nanoparticles are potential protein carriers, and that their physicochemical properties and release profile could be optimized by means of various modifications.
This paper references
Factors affecting protein release from alginate-chitosan coacervate microcapsules during production and gastric/intestinal simulation.
G. Vandenberg (2001)
Biodegradation and distribution of water-soluble chitosan in mice.
Hayato Onishi (1999)
New method for the quaternization of chitosan
A. Domard (1986)
Chitosan for enhanced intestinal permeability: prospects for derivatives soluble in neutral and basic environments.
A. Kotzé (1999)
Preparation and characterization of protein-loaded N-trimethyl chitosan nanoparticles as nasal delivery system.
M. Amidi (2006)
Protein encapsulation in biodegradable amphiphilic microspheres.
P. Bouillot (1999)
Effect of the degree of quaternisation of N-trimethyl chitosan chloride on absorption enhancement: in vivo evaluation in rat nasal epithelia.
J. Hamman (2002)
Alternative approach to the preparation of chitosan beads
C. Aral (1998)
Effect of Chitosan on the Permeability of Monolayers of Intestinal Epithelial Cells (Caco-2)
P. Artursson (2004)
Chitosan for mucosal vaccination.
I. M. van der Lubben (2001)
Trimethylated chitosan as polymeric absorption enhancer for improved peroral delivery of peptide drugs.
S. M. van der Merwe (2004)
Chitosan: a unique polysaccharide for drug delivery.
O. Felt (1998)
Basic studies on bioadhesive delivery systems for peptide and protein drugs
A. Bernkop-Schnürch (1998)
N-Trimethyl Chitosan Chloride as a Potential Absorption Enhancer Across Mucosal Surfaces: In Vitro Evaluation in Intestinal Epithelial Cells (Caco-2)
Awie R Kotzé (2004)
Preparation and NMR characterization of highly substitutedN-trimethyl chitosan chloride
A. Sieval (1998)
Preparation and modification of N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride nanoparticle as a protein carrier.
Y. Xu (2003)
Chitosan and the mucosal delivery of biotechnology drugs.
Mohamed M. Issa (2005)
Alginate/chitosan particulate systems for sodium diclofenac release.
M. González-Rodríguez (2002)
Effect of degree of quaternization of N-trimethyl chitosan chloride for enhanced transport of hydrophilic compounds across intestinal caco-2 cell monolayers.
M. Thanou (2000)
Blood compatibility and biodegradability of partially N-acylated chitosan derivatives.
K. Lee (1995)
Adsorption determines in-vitro protein release rate from biodegradable microspheres: quantitative analysis of surface area during degradation
G. Crotts (1997)
A novel approach to prepare tripolyphosphate/chitosan complex beads for controlled release drug delivery.
X. Shu (2000)
N-trimethyl chitosan chloride as absorption enhancer in oral peptide drug delivery. Development and characterization of minitablet and granule formulations.
S. M. van der Merwe (2004)
Chitosan microparticles for mucosal vaccination against diphtheria: oral and nasal efficacy studies in mice.
I. M. van der Lubben (2003)
Project acceleration — the case for quantum change
C. Cimarusti (1997)
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