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Enhanced Solubility And Stability Of PEGylated Liposomal Paclitaxel: In Vitro And In Vivo Evaluation.
T. Yang, F. Cui, Min-Koo Choi, Jei-Won Cho, S. Chung, C. Shim, Dae-Duk Kim
Published 2007 · Chemistry, Medicine
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An improved PEGylated liposomal formulation of paclitaxel has been developed with the purpose of improving the solubility of paclitaxel as well as the physicochemical stability of liposome in comparison to the current Taxol formulation. The use of 3% (v/v) Tween 80 in the hydration media was able to increase the solubility of drug. The addition of sucrose as a lyoprotectant in the freeze-drying process increased the stability of the liposome particles. There was no significant difference in the entrapment efficiency of paclitaxel between the conventional non-PEGylated liposomes and our PEGylated liposomes. Cytotoxicity in human breast cancer cell lines (MDA-MB-231 and SK-BR-3) of our paclitaxel formulation was less potent compared to Taxol after 24h incubation, but was equipotent after 72 h due to the slower release of drug from the liposome. Our PEGylated liposomes increased the biological half-life of paclitaxel from 5.05 (+/-1.52)h to 17.8 (+/-2.35)h compared to the conventional liposomes in rats. Biodistribution studies in breast cancer xenografted nude mouse model showed that our liposomes significantly decreased the uptake in reticuloendothelial system (RES)-containing organs (liver, spleen and lung) while increasing the uptake in tumor tissues after injection compared to Taxol or the conventional liposomal formulation. Moreover, the PEGylated liposome showed greater tumor growth inhibition effect in in vivo studies. Therefore, our PEGylated liposomal formulation of paclitaxel could serve as a better alternative for the passive targeting of human breast tumors.
This paper references
Paclitaxel-liposomes for intracavitary therapy of intraperitoneal P388 leukemia.
A. Sharma (1996)
Preservation of freeze-dried liposomes by trehalose.
L. Crowe (1985)
Development and characterization of a novel Cremophor EL free liposome-based paclitaxel (LEP-ETU) formulation.
J. Zhang (2005)
Lyophilized preliposomal formulation of the non-cross-resistant anthracycline annamycin: effect of surfactant on liposome formation, stability and size
Y. Zou (1996)
Effect of edge activators on the formation and transfection efficiency of ultradeformable liposomes.
Eun Hye Lee (2005)
Self-assembly and characterization of paclitaxel-loaded N-octyl-O-sulfate chitosan micellar system.
C. Zhang (2004)
Hypersensitivity reactions from taxol.
R. Weiss (1990)
Liposome Formulation of Paclitaxel with Enhanced Solubility and Stability
T. Yang (2007)
Protection of large unilamellar vesicles by trehalose during dehydration: retention of vesicle contents.
T. Madden (1985)
The choice of a suitable oligosaccharide to prevent aggregation of PEGylated nanoparticles during freeze thawing and freeze drying.
W. Hinrichs (2006)
Polymeric micelles for the solubilization and delivery of cyclosporine A: pharmacokinetics and biodistribution.
H. M. Aliabadi (2005)
Which polymers can make nanoparticulate drug carriers long-circulating?
V. Torchilin (1995)
Paclitaxel and its formulations.
A. K. Singla (2002)
A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity.
P. Twentyman (1987)
Preparation, characterization and properties of sterically stabilized paclitaxel-containing liposomes.
P. Crosasso (2000)
Preclinical pharmacokinetics of paclitaxel and docetaxel.
A. Sparreboom (1998)
Drug therapy : paclitaxel (Taxol)
E. Rowinsky (1995)
Determination of paclitaxel and metabolites in mouse plasma, tissues, urine and faeces by semi-automated reversed-phase high-performance liquid chromatography.
A. Sparreboom (1995)
Paclitaxel nanoparticles for the potential treatment of brain tumors.
J. Koziara (2004)
[Studies on the stability and some other important physical properties of polysaccharides polyphase liposome (PPL)].
Y. Zou (1989)
Pharmacokinetics of a new antitumor 3-arylisoquinoline derivative, CWJ-a-5.
K. Kim (2001)
Liposomal delivery system for taxol
M. Shieh (1997)
Liposomes targeted via two different antibodies: assay, B-cell binding and cytotoxicity.
Kimberley M Laginha (2005)
Vascular permeability in a human tumor xenograft: molecular size dependence and cutoff size.
F. Yuan (1995)
Preparation and evaluation of sterically stabilized liposomes: colloidal stability, serum stability, macrophage uptake, and toxicity.
B. Kronberg (1990)
Pharmacokinetic and cytotoxic studies of pegylated liposomal daunorubicin
H. Song (2005)
Isocratic high-performance liquid chromatographic assay of taxol in biological fluids and tissues using automated column switching.
D. Song (1995)
Preparation, characterization, cytotoxicity and pharmacokinetics of liposomes containing docetaxel.
M. Immordino (2003)
Anti-neovascular therapy by use of tumor neovasculature-targeted long-circulating liposome.
N. Maeda (2004)
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Ex-vivo/in-vitro anti-polyethylene glycol (PEG) immunoglobulin M production from murine splenic B cells stimulated by PEGylated liposome.
Amr S Abu Lila (2013)
Paclitaxel-loaded polyester nanoparticles prepared by spray-drying technology: in vitro bioactivity evaluation
P. López-Gasco (2011)
Maleimide-Functionalized Poly(2-Oxazoline)s and Their Conjugation to Elastin-Like Polypeptides.
Jonas F. Nawroth (2016)
Simultaneously enhancing the solubility and permeability of acyclovir by crystal engineering approach
Y. Yan (2013)
Receptor targeted gene delivery using folate ligand conjugated cationic liposomes.
S. Gorle (2015)
Biodistribution and Pharmacokinetic Analysis of Paclitaxel and Ceramide Administered in Multifunctional Polymer-Blend Nanoparticles in Drug Resistant Breast Cancer Model
Lilian E van Vlerken (2008)
Distribution of Melaleuca alternifolia essential oil in liposomes with Tween 80 addition and enhancement of in vitro antimicrobial effect
Yan Ge (2016)
Drug-Induced Morphology Switch in Drug Delivery Systems Based on Poly(2-oxazoline)s
A. Schulz (2014)
Tumor-targeted paclitaxel delivery and enhanced penetration using TAT-decorated liposomes comprising redox-responsive poly(ethylene glycol).
H. Fu (2015)
Targeted theranostic liposomes: rifampicin and ofloxacin loaded pegylated liposomes for theranostic application in mycobacterial infections
A. Kaul (2016)
Development and Characterization of Nanoliposomal Hydroxyurea Against BT-474 Breast Cancer Cells
A. Akbari (2020)
Enhanced antitumor effect of targeted nanoliposomal bleomycin
M. Chiani (2017)
The choice of anti-tumor strategies based on micromolecules or drug loading function of biomaterials.
C. Liu (2020)
Design, synthesis and in vivo antitumor efficacy of novel eight-arm-polyethylene glycol–pterostilbene prodrugs
K. Liu (2015)
Effect of liposomal celecoxib on proliferation of colon cancer cell and inhibition of DMBA-induced tumor in rat model
V. Perumal (2011)
Polymeric nanocapsules embedded with ultra-small silver nanoclusters for synergistic pharmacology and improved oral delivery of Docetaxel
M. Sohail (2018)
Amphiphilic Triblock Copolymers from Poly(2‐oxazoline) with Different Hydrophobic Blocks: Changes of the Micellar Structures upon Addition of a Strongly Hydrophobic Cancer Drug
Sebastian Jaksch (2016)
Cell penetrating peptide amphiphile integrated liposomal systems for enhanced delivery of anticancer drugs to tumor cells.
M. Sardan (2013)
Improved Dissolution and Bioavailability of Eprosartan Mesylate Formulated as Solid Dispersions using Conventional Methods
P. Dangre (2016)
Injectable liposomal formulations of opiorphin as a new therapeutic strategy in pain management
N. Mennini (2015)
Synthesis of a novel, sequentially active-targeted drug delivery nanoplatform for breast cancer therapy.
Arpan Satsangi (2015)
Development of a fast and efficient liposomal drug loading technology for poorly water soluble drugs : formulation, characterization, and potential applications
Wei-Lun Tang (2018)
Preparation and Cytotoxicity Effect of Anti-Hepatocellular Carcinoma Scfv Immunoliposome on Hepatocarcinoma Cell in Vitro
G-H. Zhang (2010)
Optimization of drug loading to improve physical stability of paclitaxel-loaded long-circulating liposomes
Vinayagam Kannan (2015)
Synthesis of sodium citrate modified Fe 3 O 4 NPs The hydrophilic Fe
Z. Cao (2014)
Practical Liposomal Formulation for Taxanes with Polyethoxylated Castor Oil and Ethanol with Complete Encapsulation Efficiency and High Loading Efficiency
Tsukasa Shigehiro (2017)
Extraction and RP-HPLC determination of taxol
Rakesh K Tekade (2013)
Extraction and RP-HPLC determination of taxol in rat plasma, cell culture and quality control samples
Rakesh K Tekade (2013)
Liposomes for targeted drug delivery in cancer therapy
M. Shah (2017)
Synthesis and Characterization of Cleavable Core-Cross-Linked Micelles Based on Amphiphilic Block Copolypeptoids as Smart Drug Carriers.
A. Li (2016)
Development of a Rapidly Dissolvable Oral Pediatric Formulation for Mefloquine Using Liposomes.
Wei-Lun Tang (2017)
Precision combination therapy for triple negative breast cancer via biomimetic polydopamine polymer core-shell nanostructures.
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