🙌 New: Upload your document to have your APA citations automatically checked
Online citations, reference lists, and bibliographies.
← Back to Search

Integrated Analyses Of Phenotype, Phytohormone, And Transcriptome To Elucidate The Mechanism Governing Internode Elongation In Two Contrasting Elephant Grass (Cenchrus Purpureus) Cultivars

Qi Yan, Jie Li, Liyan Lu, Lijuan Gao, Lai Dawei, Yao Na, Yi Xianfeng, Zhuyue Wu, Lai Zhiqiang, Jiyu Zhang
Published 2021 · Biology

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Abstract In view of the global energy crisis and climate change, elephant grass (Cenchrus purpureus (Schumach.) Morrone syn. Pennisetum purpureum Schumach.) represents a useful lignocellulosic biofuel feed stock due to its high biomass yield and persistence. Internode elongation is an important factor that affects the productivity of energy grass. Elucidating the molecular mechanism governing internode elongation may facilitate the development of molecular improvement strategies for energy grass, but this mechanism has not been thoroughly investigated in elephant grass to date. In this study, the phenotypic evaluation of six cultivars of elephant grass showed that the plant height positively correlated with internode length, rather than internode number. The dwarf cultivar C. purpureus cv. Mott (CpMott) and the tall cultivar C. purpureus cv. GuiMinYin (CpGMY) exhibited significantly different plant height and internode length but not internode number. Analyses of transcription profiles and gibberellin concentrations from the shoot tips of the two cultivars showed that the endogenous bioactive gibberellin A1 (GA1) concentrations was markedly decreased whilst some of representative gibberellin biosynthetic enzymes were downregulated in the CpMott, such as GA20-oxidase (GA20ox) and ent-kaurene synthase (KS). Exogenous application of gibberellin A3 (GA3) indicated that CpMott is a gibberellin-sensitive dwarf cultivar. In addition, the gibberellin-deficiency of CpMott reduced the lignin content by inhibiting the transcript levels enzyme genes in lignin biosynthesis, while induced the expression levels of genes related to abiotic stress. Overall, our results may serve to elucidate the molecular mechanism underlying elephant grass internode elongation and facilitate the development of thus grass into a more efficient energy source.
This paper references
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
K. Livak (2001)
The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway.
C. Helliwell (2001)
Accumulation of Phosphorylated Repressor for Gibberellin Signaling in an F-box Mutant
A. Sasaki (2003)
Impact of Altered Gibberellin Metabolism on Biomass Accumulation, Lignin Biosynthesis, and Photosynthesis in Transgenic Tobacco Plants
S. Biemelt (2004)
Relationship between gibberellins, height, and stress tolerance in barley (Hordeum vulgare L.) seedlings
S. Sarkar (2004)
The Molecular Genetics of Crop Domestication
J. Doebley (2006)
From dwarves to giants? Plant height manipulation for biomass yield.
M. G. Salas Fernandez (2009)
Gene ontology analysis for RNA-seq: accounting for selection bias
Matthew D. Young (2010)
The simultaneous repression of CCR and CAD, two enzymes of the lignin biosynthetic pathway, results in sterility and dwarfism in Arabidopsis thaliana.
J. Thévenin (2011)
Overexpression of AtDREB1A Causes a Severe Dwarf Phenotype by Decreasing Endogenous Gibberellin Levels in Soybean [Glycine max (L.) Merr.]
H. Suo (2012)
The Mitochondrial Phosphate Transporters Modulate Plant Responses to Salt Stress via Affecting ATP and Gibberellin Metabolism in Arabidopsis thaliana
W. Zhu (2012)
Functional characterization of cinnamyl alcohol dehydrogenase and caffeic acid O-methyltransferase in Brachypodium distachyon
Gina M. Trabucco (2013)
Advances in biotechnology and genomics of switchgrass
M. Nageswara-Rao (2013)
The Maize DWARF1 Encodes a Gibberellin 3-Oxidase and Is Dual Localized to the Nucleus and Cytosol1[W]
Yi Chen (2014)
Miscanthus as cellulosic biomass for bioethanol production
Wen‐Chien Lee (2015)
Identification and overexpression of gibberellin 2-oxidase (GA2ox) in switchgrass (Panicum virgatum L.) for improved plant architecture and reduced biomass recalcitrance.
W. Wuddineh (2015)
A state-of-the-art review of biomass torrefaction, densification and applications
Wei-hsin Chen (2015)
Use of elephant grass (Pennisetum purpureum) acid hydrolysate for microbial oil production by Trichosporon cutaneum
Xue-fang Chen (2016)
Overexpression of Jatropha Gibberellin 2-oxidase 6 (JcGA2ox6) Induces Dwarfism and Smaller Leaves, Flowers and Fruits in Arabidopsis and Jatropha
Yingjie Hu (2017)
Elephant grass ecotypes for bioenergy production via direct combustion of biomass.
J. Rocha (2017)
Ectopic expression of specific GA2 oxidase mutants promotes yield and stress tolerance in rice
S. Lo (2017)
Genetic insights into elephantgrass persistence for bioenergy purpose
J. Rocha (2018)
Production and Bromatological Characteristics of Elephant Grass—Cenchrus purpureus (Schumach.) Morrone—Planted Under Application of Industrial Biosolid and Chemical Fertilization
Talles Iwasawa Neves (2018)
Characterization of GA20ox genes in tall and dwarf types coconut (Cocos nucifera L.)
T. Boonkaew (2018)
Heterologous expression of a novel Poa pratensis gibberellin 2-oxidase gene, PpGA2ox, caused dwarfism, late flowering, and increased chlorophyll accumulation in Arabidopsis
Penghui Tan (2018)
Genotyping by sequencing provides new insights into the diversity of Napier grass (Cenchrus purpureus) and reveals variation in genome-wide LD patterns between collections
M. Muktar (2019)
A single nucleotide mutation in GID1c disrupts its interaction with DELLA1 and causes a GA‐insensitive dwarf phenotype in peach
Jun Cheng (2019)
Expression of wheat gibberellins 2-oxidase gene induced dwarf or semi-dwarf phenotype in rice
S. Tang (2019)
SLR1 inhibits MOC1 degradation to coordinate tiller number and plant height in rice
Zhigang Liao (2019)
The future of bioenergy
W. Reid (2019)
Valorization of energy crops as a source for nanocellulose production – Current knowledge and future prospects
J. Pires (2019)
Effect of Elephant Grass Genotypes to Bioenergy Production
H. Favare (2019)
Enzymatic sugar production from elephant grass and reed straw through pretreatments and hydrolysis with addition of thioredoxin-His-S
Xianqin Lu (2019)
A point mutation resulting in a 13 bp deletion in the coding sequence of Cldf leads to a GA-deficient dwarf phenotype in watermelon
Chunhua Wei (2019)
The role of gibberellin synthase gene GhGA2ox1 in upland cotton (Gossypium hirsutum L.) responses to drought and salt stress
Jianbin Shi (2019)
The grain yield modulator miR156 regulates seed dormancy through the gibberellin pathway in rice
Chunbo Miao (2019)
Linking phenylpropanoid metabolism, lignin deposition, and plant growth inhibition.
Fabiola Muro-Villanueva (2019)
PbGA20ox2 Regulates Fruit Set and Induces Parthenocarpy by Enhancing GA4 Content
Huibin Wang (2020)
Overexpression of BnGA2ox2, a rapeseed gibberellin 2-oxidase, causes dwarfism and increased chlorophyll and anthocyanin accumulation in Arabidopsis and rapeseed
Jindong Yan (2020)
Overgrowth mutants determine the causal role of GA2oxidaseA13 in Rht12 dwarfism of wheat.
W. Buss (2020)
Targeted mutation of GA20ox-2 gene using CRISPR/Cas9 system generated semi-dwarf phenotype in rice
T. Santoso (2020)
The elephant grass (Cenchrus purpureus) genome provides insights into anthocyanidin accumulation and fast growth
Qichuan Yan (2020)
Mutations on ent-kaurene oxidase 1 encoding gene attenuate its enzyme activity of catalyzing the reaction from ent-kaurene to ent-kaurenoic acid and lead to delayed germination in rice
H. Zhang (2020)
Dwarf and Increased Branching 1 controls plant height and axillary bud outgrowth in Medicago truncatula.
Xiaojia Zhang (2020)
SLENDER RICE1 and Oryza sativa INDETERMINATE DOMAIN2 Regulating OsmiR396 Are Involved in Stem Elongation1
Yuzhu Lu (2020)
MicroRNA biogenesis in plant
Zihan Gao (2020)
Copalyl Diphosphate Synthase Mutation Improved Salt Tolerance in Maize (Zea mays. L) via Enhancing Vacuolar Na+ Sequestration and Maintaining ROS Homeostasis
Yushi Zhang (2020)

Semantic Scholar Logo Some data provided by SemanticScholar