Online citations, reference lists, and bibliographies.
← Back to Search

Effective Protein Release From PEG/PLA Nano-particles Produced By Compressed Gas Anti-solvent Precipitation Techniques.

P. Caliceti, S. Salmaso, N. Elvassore, A. Bertucco
Published 2004 · Medicine, Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
Homogeneous PLA/insulin solutions containing different amounts of 350, 750 or 1900 Da PEG (0-75 wt.% PEG) were processed by semi-continuous compressed CO2 anti-solvent precipitation to fabricate protein-loaded polymeric nano-particles. Proper operative conditions (temperature, pressure, CO2 flow rate and washing time) yielded more than 70% product recovery. Scanning electron microscopy, transmission electron microscopy and light scattering demonstrated that spherical, smooth surfaced particles with size below 1 microm could be obtained. X-ray diffraction analysis showed that the gas anti-solvent process modifies the polylactide crystalline state. PEG concentration and molecular weight were found to affect both optimal operative conditions and morphological and biopharmaceutical properties of the final product. Insulin loading yield dropped from 95% to 65% by increasing the 1900 Da PEG content from 0 to 75 wt.% or the PEG molecular weight from 350 to 1900 Da. The release rate increased significantly as the PEG content in the formulation increases. After 3-month incubation the drug released raised from 10% to 100% by increasing the 1900 Da PEG content from 23 to 7 wt.%. Formulations containing the same 350, 750 or 1900 Da PEG amount (67 wt.% PEG) displayed similar release profiles. Insulin release was found to take place by diffusion mechanism, despite the observation of matrix degradation.
This paper references
10.1021/JS950482Q
Precipitation of proteins in supercritical carbon dioxide.
M. Winters (1996)
10.1023/A:1011009117586
Stabilization and Controlled Release of Bovine Serum Albumin Encapsulated in Poly(D, L-lactide) and Poly(ethylene glycol) Microsphere Blends
W. Jiang (2004)
10.1021/JS9700661
Pharmaceutical processing with supercritical carbon dioxide.
B. Subramaniam (1997)
10.1021/JS980237H
Encapsulation of lysozyme in a biodegradable polymer by precipitation with a vapor-over-liquid antisolvent.
T. J. Young (1999)
10.1016/0168-3659(87)90034-4
A simple equation for description of solute release I. Fickian and non-fickian release from non-swellable devices in the form of slabs, spheres, cylinders or discs
P. L. Ritger (1987)
10.1016/S0896-8446(00)00054-1
Numerical modeling of mass transfer in the supercritical antisolvent process: miscible conditions
Jane O. Werling (2000)
10.1016/S0264-410X(98)00229-1
The stability and immunogenicity of a protein antigen encapsulated in biodegradable microparticles based on blends of lactide polymers and polyethylene glycol.
E. Lavelle (1999)
10.1002/AIC.690480207
Protein nanoparticles formation by supercritical antisolvent with enhanced mass transfer
P. Chattopadhyay (2002)
10.1016/S0928-0987(99)00079-2
A new method for preparing biodegradable microparticles and entrapment of hydrocortisone in DL-PLG microparticles using supercritical fluids.
R. Ghaderi (2000)
10.1002/MACP.1992.021930704
Structure and crystallization kinetics of poly(L-lactic acid)
C. Marega (1992)
10.1002/BIT.260410308
Formation of microparticulate protein powder using a supercritical fluid antisolvent
S. Yeo (1993)
10.1021/IE0004904
Production of Protein-Loaded Polymeric Microcapsules by Compressed CO2 in a Mixed Solvent
N. Elvassore (2001)
10.1016/S0896-8446(98)00129-6
SUPERCRITICAL ANTISOLVENT PRECIPITATION OF MICRO- AND NANO-PARTICLES
E. Reverchon (1999)
10.1002/JPS.1113
Production of insulin-loaded poly(ethylene glycol)/poly(l-lactide) (PEG/PLA) nanoparticles by gas antisolvent techniques.
N. Elvassore (2001)
10.1002/1097-0290(20010105)72:1<12::AID-BIT2>3.0.CO;2-Z
The formation of plasmid DNA loaded pharmaceutical powders using supercritical fluid technology.
M. Tservistas (2001)
10.1021/IE010943K
Supercritical-assisted atomization to produce micro- and/or nanoparticles of controlled size and distribution
E. Reverchon (2002)
10.1021/IE020205B
Supercritical CO2 Based Production of Magnetically Responsive Micro- and Nanoparticles for Drug Targeting
P. Chattopadhyay (2002)
10.1021/IE020070+
Modeling the Gas Antisolvent Recrystallization Process
G. Muhrer (2002)
10.1016/0300-9629(93)90342-2
Peptide and protein drug delivery
V. Lee (1991)
10.1615/CRITREVTHERDRUGCARRIERSYST.V12.I1.10
Biodegradable microspheres in drug delivery.
H. Okada (1995)
10.5860/choice.35-0927
Diffusion: Mass Transfer in Fluid Systems
E. Cussler (1984)
10.1016/S0958-1669(00)00202-0
Emerging protein delivery methods.
J. Cleland (2001)
10.1016/S0896-8446(01)00064-X
Particle design using supercritical fluids: Literature and patent survey
Jennifer Jung (2001)
10.1002/JPS.1113
Production of insulin loaded PEG/PLA nano-particles by gas anti-solvent techniques
N. Elvassore (2001)
Controlled drug delivery : challenges and strategies
K. Park (1997)
10.1002/AIC.690460418
Microencapsulation of proteins by rapid expansion of supercritical solution with a nonsolvent
K. Mishima (2000)
10.1016/0003-2697(80)90492-3
A method for the estimation of polyethylene glycol in plasma protein fractions.
G. Sims (1980)
10.1021/JS970419W
Preparation and in vitro characterization of gentamycin-impregnated biodegradable beads suitable for treatment of osteomyelitis.
J. D. Meyer (1998)



This paper is referenced by
10.1016/j.ijpharm.2009.06.014
Biopharmaceutical characterisation of insulin and recombinant human growth hormone loaded lipid submicron particles produced by supercritical gas micro-atomisation.
S. Salmaso (2009)
10.1002/jps.23998
Next generation drying technologies for pharmaceutical applications.
Robert Henry Walters (2014)
10.1016/j.ijpharm.2012.06.022
Preparation and characterization of 5-fluorouracil-loaded PLLA-PEG/PEG nanoparticles by a novel supercritical CO2 technique.
Cheng Zhang (2012)
10.1002/9783527670260.CH5
ScCO2 Techniques for Surface Modification of Micro‐ and Nanoparticles
P. Subra-Paternault (2015)
10.1016/J.SUPFLU.2006.05.006
Production of micro- and nano-composite particles by supercritical carbon dioxide
M. Bahrami (2007)
10.1016/J.IJPHARM.2007.05.008
Theophylline formulation by supercritical antisolvents.
C. Roy (2007)
10.1155/2013/103527
Microencapsulation for the Therapeutic Delivery of Drugs, Live Mammalian and Bacterial Cells, and Other Biopharmaceutics: Current Status and Future Directions
C. Tomaro-Duchesneau (2013)
10.1016/j.jconrel.2009.12.009
Regulated non-viral gene delivery from coaxial electrospun fiber mesh scaffolds.
Anita Saraf (2010)
Regulated release of a novel non-viral gene delivery vector from electrospun coaxial fiber mesh scaffolds
Anita Saraf (2010)
10.3109/10837450.2012.726998
Supercritical fluid technology: a promising approach in pharmaceutical research
Priti Girotra (2013)
A study of some lipid-based drug delivery systems
A. Ramadan (2010)
10.1016/J.ADDR.2007.02.002
Application of supercritical fluid to preparation of powders of high-molecular weight drugs for inhalation.
H. Okamoto (2008)
10.1016/J.EURPOLYMJ.2007.06.045
New emerging trends in synthetic biodegradable polymers – Polylactide: A critique
A. P. Gupta (2007)
10.1016/j.colsurfb.2008.12.031
Strategic approaches for improving entrapment of hydrophilic peptide drugs by lipid nanoparticles.
Hong Yuan (2009)
Formulation of stable protein powders by supercritical fluid drying
N. Jovanović (2007)
10.1016/j.exer.2013.04.007
Biodegradable nanoparticles for controlled subconjunctival delivery of latanoprost acid: in vitro and in vivo evaluation. Preliminary results.
Athanassios Giarmoukakis (2013)
10.1201/B12950-14
Protein Drug Delivery to Retina and Choroid
H. Rivers (2012)
10.2147/IJN.S19765
Novel PEG-graft-PLA nanoparticles with the potential for encapsulation and controlled release of hydrophobic and hydrophilic medications in aqueous medium
B. Wang (2011)
Fabrication of controlled release devices for anticancer agents using supercritical antisolvent method
L. Y. Lee (2005)
10.1016/J.SUPFLU.2006.09.008
Synthesis of controlled release device with supercritical CO2 and co-solvent
Joshua R. Bush (2007)
10.1016/J.ADDR.2006.07.010
Particle engineering techniques for inhaled biopharmaceuticals.
S. Shoyele (2006)
10.1007/978-3-030-44552-2_2
Supercritical Fluid Techniques to Fabricate Efficient Nanoencapsulated Food-Grade Materials
Umar Garba (2020)
10.1007/s13346-015-0225-3
Supercritical synthesis of poly (2-dimethylaminoethyl methacrylate)/ferrite nanocomposites for real-time monitoring of protein release
Gunjan Bisht (2015)
10.1016/j.addr.2016.05.020
PLA micro- and nano-particles.
B. K. Lee (2016)
10.4172/2157-7439.1000259
Design and Evaluation of Hydrophobic Ion-Pairing Complexation ofLysozyme with Sodium Dodecyl Sulfate for Improved Encapsulation ofHydrophilic Peptides/Proteins by Lipid-Polymer Hybrid Nanoparticles
B. Devrim (2015)
10.3390/ma3031928
Targeted Delivery of Protein Drugs by Nanocarriers
R. Solaro (2010)
10.1016/J.SUPFLU.2007.02.008
Glucose oxidase immobilization on conducting polymers in supercritical CO2 environment: An exploratory study
Defne Kayrak-Talay (2007)
10.2147/IJN.S213613
Tailoring of PEGylated bilosomes for promoting the transdermal delivery of olmesartan medoxomil: in-vitro characterization, ex-vivo permeation and in-vivo assessment
Rofida Albash (2019)
10.1016/J.IJPHARM.2004.09.003
Nisin-loaded poly-L-lactide nano-particles produced by CO2 anti-solvent precipitation for sustained antimicrobial activity.
S. Salmaso (2004)
10.1016/J.ADDR.2006.12.001
Applications of supercritical CO2 in the fabrication of polymer systems for drug delivery and tissue engineering.
O. R. Davies (2008)
10.1039/C4TB01319K
Preparation of polymeric particles in CO2 medium using non-toxic solvents: discussions on the mechanism of particle formation.
My-Kien Tran (2015)
10.1016/J.SUPFLU.2010.05.013
Production of lipid microparticles containing bioactive molecules functionalized with PEG
K. Vezzù (2010)
See more
Semantic Scholar Logo Some data provided by SemanticScholar