← Back to Search
Development And Characterisation Of Chitosan Nanoparticles For SiRNA Delivery.
Haliza Katas, H. O. Alpar
Published 2006 · Chemistry, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
Gene silencing mediated by double-stranded small interfering RNA (siRNA) has been widely investigated as a potential therapeutic approach for diseases with genetic defects. The use of siRNA, however, is hampered by its rapid degradation and poor cellular uptake into cells in vitro or in vivo. Therefore, we have explored chitosan as a siRNA vector due to its advantages such as low toxicity, biodegradability and biocompatibility. Chitosan nanoparticles were prepared by two methods of ionic cross-linking, simple complexation and ionic gelation using sodium tripolyphosphate (TPP). Both methods produced nanosize particles, less than 500 nm depending on type, molecular weight as well as concentration of chitosan. In the case of ionic gelation, two further factors, namely chitosan to TPP weight ratio and pH, affected the particle size. In vitro studies in two types of cells lines, CHO K1 and HEK 293, have revealed that preparation method of siRNA association to the chitosan plays an important role on the silencing effect. Chitosan-TPP nanoparticles with entrapped siRNA are shown to be better vectors as siRNA delivery vehicles compared to chitosan-siRNA complexes possibly due to their high binding capacity and loading efficiency. Therefore, chitosan-TPP nanoparticles show much potential as viable vector candidates for safer and cost-effective siRNA delivery.
This paper references
Optimization of an siRNA‐expression system with an improved hairpin and its significant suppressive effects in mammalian cells
M. Miyagishi (2004)
Sustained expression in mammalian cells with DNA complexed with chitosan nanoparticles.
X. W. Li (2003)
siRNA delivery technologies for mammalian systems
D. Rozema (2003)
The use of chitosan as a condensing agent to enhance emulsion-mediated gene transfer.
M. Lee (2005)
Physicochemical characterization of chitosan nanoparticles: electrokinetic and stability behavior.
T. López-León (2005)
Light scattering studies on DNA condensation.
V. Bloomfield (1991)
Lipidic carriers of RNA/DNA oligonucleotides and polynucleotides: what a difference a formulation makes!
M. Keller (2005)
The influence of multivalent phosphate structure on the properties of ionically cross-linked chitosan films for controlled drug release.
X. Shu (2002)
RNAi-mediated gene-targeting through systemic application of polyethylenimine (PEI)-complexed siRNA in vivo
B. Urban-Klein (2005)
Cationic liposome-mediated delivery of siRNAs in adult mice.
M. Sioud (2003)
Gene Silencing Nucleic Acids Designed by Scanning Arrays: Anti-EGFR Activity of siRNA, Ribozyme and DNA Enzymes Targeting a Single Hybridization-accessible Region using the Same Delivery System
G. Beale (2003)
The controlled intravenous delivery of drugs using PEG-coated sterically stabilized nanospheres.
R. Gref (1995)
Transfection efficiency of chitosan vectors: effect of polymer molecular weight and degree of deacetylation.
M. Huang (2005)
Synergistic effect of poly(ethylenimine) on the transfection efficiency of galactosylated chitosan/DNA complexes.
T. Kim (2005)
Albumin-protamine-oligonucleotide-nanoparticles as a new antisense delivery system. Part 2: cellular uptake and effect.
J. Weyermann (2005)
Effect of solvent on the preparation of surfactant-free poly(DL-lactide-co-glycolide) nanoparticles and norfloxacin release characteristics.
H. Jeon (2000)
Pharmaceutical Prospects for RNA Interference
R. Schiffelers (2004)
Targeting delivery of oligonucleotide and plasmid DNA to hepatocyte via galactosylated chitosan vector.
Shuying Gao (2005)
Stabilized plasmid-lipid particles for systemic gene therapy
P. Tam (2000)
RNA interference in mammalian cells by chemically-modified RNA.
D. A. Braasch (2003)
In vitro uptake of polystyrene microspheres: effect of particle size, cell line and cell density.
W. Zauner (2001)
Condensation of DNA by multivalent cations: Considerations on mechanism
V. Bloomfield (1991)
Sustained Polymeric Delivery of Gene Silencing Antisense ODNs, siRNA, DNAzymes and Ribozymes: In Vitro and In Vivo Studies
A. Khan (2004)
Phosphorothioate antisense oligodeoxynucleotides: questions of specificity.
C. Stein (1996)
Gene silencing by systemic delivery of synthetic siRNAs in adult mice.
D. Sørensen (2003)
Polysaccharide colloidal particles as delivery systems for macromolecules.
K. Janes (2001)
Biodegradable nanoparticles for drug and gene delivery to cells and tissue.
J. Panyam (2003)
PLGA–PEI nanoparticles for gene delivery to pulmonary epithelium
M. Bivas-Benita (2004)
Modulation of surface charge, particle size and morphological properties of chitosan-TPP nanoparticles intended for gene delivery.
Quan Gan (2005)
Block copolymer-coated calcium phosphate nanoparticles sensing intracellular environment for oligodeoxynucleotide and siRNA delivery.
Y. Kakizawa (2004)
This paper is referenced by
Design of a droplet based microfluidic reactor to synthesize chitosan coated iron oxide nanoparticles
M. A. Wahab (2019)
RES-loaded pegylated CS NPs: for efficient ocular delivery.
Saravanakumar Pandian (2017)
Electrochemical examination of ability of dsDNA/PAM composites for storing and releasing of doxorubicin.
Ewelina Zabost (2016)
Identification of critical points in colloidal delivery systems for intravenous administration and intracellular delivery of nucleic acids as therapeutic agents
Faculdade de Farmácia (2012)
Facile preparation of pH-responsive k-Carrageenan/tramadol loaded UiO-66 bio-nanocomposite hydrogel beads as a nontoxic oral delivery vehicle
S. Javanbakht (2019)
Bleomycin Loaded Magnetic Chitosan Nanoparticles as Multifunctional Nanocarriers
Doga Kavaz (2010)
Anti-epidermal growth factor receptor siRNA carried by chitosan-transacylated lipid nanocapsules increases sensitivity of glioblastoma cells to temozolomide
Khaled Messaoudi (2014)
5-aminopyrazole-conjugated gelatin hydrogel: A controlled 5-fluorouracil delivery system for rectal administration
M. Nazeri (2020)
Chitosan-Conjugated DNA Nanoparticle Delivery Systems for Gene Therapy
R. Jayakumar (2010)
Smart siRNA delivery systems based on polymeric nanoassemblies and nanoparticles.
A. Tamura (2010)
A Combinatorial Library of Bi-functional Polymeric Vectors for siRNA Delivery In Vitro
Jeisa M. Pelet (2012)
Natural Polysaccharides for siRNA Delivery: Nanocarriers Based on Chitosan, Hyaluronic Acid, and Their Derivatives
Inés Serrano-Sevilla (2019)
Chitosan-Thiamine Pyrophosphate as a Novel Carrier for siRNA Delivery
T. Rojanarata (2008)
Antifungal Activity of Chitosan Nanoparticles and Correlation with Their Physical Properties
Ling Yien Ing (2012)
Development of pH-responsive nanocarriers using trimethylchitosans and methacrylic acid copolymer for siRNA delivery.
V. Dehousse (2010)
Kinetics and functional effectiveness of nisin loaded antimicrobial packaging film based on chitosan/poly(vinyl alcohol).
H. Wang (2015)
Short interfering RNA therapeutics: nanocarriers, prospects and limitations.
N. Mehrotra (2015)
Hydrocaffeic acid-chitosan nanoparticles with enhanced stability, mucoadhesion and permeation properties.
G. M. Soliman (2014)
Candesartan-graft-polyethyleneimine cationic micelles for effective co-delivery of drug and gene in anti-angiogenic lung cancer therapy
S. Hao (2015)
Thérapie génique à l'aide de nanocapsules lipidiques PEGylées
Marie Morille (2009)
Chitosan-based formulations for delivery of DNA and siRNA.
S. Mao (2010)
Enhanced endosomal/lysosomal escape by distearoyl phosphoethanolamine-polycarboxybetaine lipid for systemic delivery of siRNA.
Y. Li (2014)
Encapsulation of dsRNA GIH (Gonad Inhibiting Hormone) Using Chitosan Nanoparticle With Several Physiochemical Optimizations
D. Crysnanto (2015)
Nanoparticulate systems for polynucleotide delivery
Ashwin Basarkar (2007)
Functional RNA delivery targeted to dendritic cells by synthetic nanoparticles.
K. Mccullough (2012)
Evaluation Of Effect Of Two Different Functionalized Nanoparticle Photodynamic Therapy On Nanohardness Of Root Dentin-An In Vitro Study.
Shesla Musthalib (2020)
Non-viral Gene Delivery Methods for Bone and Joints
Benjamin Gantenbein (2020)
Construction and characterization of Gal-chitosan graft methoxy poly (ethylene glycol) (Gal-CS-mPEG) nanoparticles as efficient gene carrier
Jiting Jin (2017)
Use of biotinylated chitosan for substrate-mediated gene delivery.
Wei-Wen Hu (2012)
Chitosan—A versatile semi-synthetic polymer in biomedical applications
M. Dash (2011)
Comparison of chitosan/siRNA and trimethylchitosan/siRNA complexes behaviour in vitro.
V. Dehousse (2010)
Chitosan and chitosan derivatives in drug delivery and tissue engineering
R. Riva (2011)See more