Online citations, reference lists, and bibliographies.
← Back to Search

Reducing Properties, Energy Efficiency And Carbohydrate Metabolism In Hyperhydric And Normal Carnation Shoots Cultured In Vitro: A Hypoxia Stress?

Shady Saher, N. Fernández-García, A. Piqueras, E. Hellín, E. Olmos
Published 2005 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Hyperhydricity is considered as a physiological disorder that can be induced by different stressing conditions. In the present work we have studied the metabolic and energetic states of hyperhydric carnation shoots. We have evaluated the hypothesis that hypoxia stress is the main factor affecting the metabolism of hyperhydric leaves. Our results indicate a low level of ATP in hyperhydric tissues, but only slight modifications in pyridine nucleotide contents. Concurrently, the glucose-6-phosphate dehydrogenase (G-6-PDH; EC 1.1.1.49) activity in hyperhydric leaves was increased but glucokinase (GK; EC 2.7.1.2) activity was unchanged. We have observed that the metabolism of pyruvate was altered in hyperhydric tissues by the induction of pyruvate synthesis via NADP-dependent malic enzyme (EC 1.1.1.40). The enzymes of the fermentative metabolism pyruvate decarboxylase (PDC; EC 4.1.1.1) and alcohol dehydrogenase (ADH; EC 1.1.1.1) were highly increased in hyperhydric leaves. Sucrose metabolism was modified in hyperhydric leaves with a high increase in the activity of both synthesis and catabolic enzymes. The analysis of the sucrose, glucose and fructose contents indicated that all of these sugars were accumulated in hyperhydric leaves. However, the pinitol content was drastically decreased in hyperhydric leaves. We consider that these results suggest that hyperhydric leaves of carnation have adapted to hypoxia stress conditions by the induction of the oxidative pentose phosphate and fermentative pathways.
This paper references
10.1111/J.0031-9317.2004.0219.X
Hyperhydricity in micropropagated carnation shoots: the role of oxidative stress.
Shady Saher (2004)
10.1071/PP98095_ER
Review: Mechanisms of anoxia tolerance in plants. I. Growth, survival and anaerobic catabolism.
J. Gibbs (2003)
10.1023/A:1006253615894
Induction of alcohol dehydrogenase by plant hormones in alfalfa seedlings
H. Kato-Noguchi (2004)
10.1016/S0168-9452(01)00362-4
Are hyperhydric shoots of Prunus avium L. energy deficient?
T. Franck (2001)
10.1146/ANNUREV.ARPLANT.47.1.185
THE ORGANIZATION AND REGULATION OF PLANT GLYCOLYSIS.
W. Plaxton (1996)
10.1016/S0168-9452(97)00214-8
The subcellular localization of peroxidase and the implication of oxidative stress in hyperhydrated leaves of regenerated carnation plants
E. Olmos (1997)
10.1016/S0981-9428(02)01434-1
Pyruvate accumulation during phosphate deficiency stress of bean roots
I. Juszczuk (2002)
10.1104/pp.111.1.187
Glycolytic Flux and Hexokinase Activities in Anoxic Maize Root Tips Acclimated by Hypoxic Pretreatment
J. Bouny (1996)
Plant Physiology, Biochemistry and Molecular Biology
D. Dennis (1990)
10.1093/JXB/40.10.1093
Pinitol, a Compatible Solute in Mesembryanthemum crystallinum L.?
M. Paul (1989)
10.1016/S0304-4238(98)00096-X
Ultrastructural differences of hyperhydric and normal leaves from regenerated carnation plants
E. Olmos (1998)
10.1104/pp.102.016907
The Pyruvate decarboxylase1 Gene of Arabidopsis Is Required during Anoxia But Not Other Environmental Stresses[w]
O. Kürsteiner (2003)
10.21273/JASHS.122.1.107
Effects of Low-oxygen Atmosphere on Ethanolic Fermentation in Fresh-cut Carrots
H. Kato-Noguchi (1997)
10.1016/J.PLAPHY.2005.01.017
Pectin methyl esterases and pectins in normal and hyperhydric shoots of carnation cultured in vitro.
Shady Saher (2005)
10.1016/S0168-9452(96)04483-4
Mechanisms of salt tolerance in a cell line of Pisum sativum: biochemical and physiological aspects
E. Olmos (1996)
10.1016/0925-5214(95)00044-5
Effects of hypoxia on cut carnation flowers (Dianthus caryophyllus L.): longevity, ability to survive under anoxia, and activities of alcohol dehydrogenase and pyruvate kinase
X. Chen (1996)
10.1016/B978-0-12-461013-2.50010-1
The enzymes in C4 photosynthesis
A. Ashton (1990)
10.1007/BF01280717
Position of water in vitrified plants visualised by NMR imaging
Karleen Gribble (2005)
10.1104/PP.123.4.1611
Induction of a C(4)-like mechanism of CO(2) fixation in Egeria densa, a submersed aquatic species.
P. Casati (2000)
10.1007/BF00024782
Protective enzymatic systems against activated oxygen species compared in normal and vitrified shoots of Prunus avium L. L. raised in vitro
T. Franck (2004)
10.1007/s11240-004-4043-4
Prevention of hyperhydricity in micropropagated carnation shoots by bottom cooling: implications of oxidative stress
Sadhy Saher (2004)
10.5860/choice.38-3895
Biochemistry & Molecular Biology of Plants
B. Buchanan (2002)
10.1016/0031-9422(95)00978-7
Lactate dehydrogenase in plants: Distribution and function
P. O'Carra (1996)
10.1016/S0176-1617(98)80160-0
Reducing properties, and markers of lipid peroxidation in normal and hyperhydrating shoots of Prunus avium L.
T. Franck (1998)
10.1023/A:1020835304842
Concepts in plant stress physiology. Application to plant tissue cultures
T. Gaspar (2004)
10.1104/PP.86.1.61
Metabolic Acclimation to Anoxia Induced by Low (2-4 kPa Partial Pressure) Oxygen Pretreatment (Hypoxia) in Root Tips of Zea mays.
P. Saglio (1988)
10.1007/BF00197056
Effect of anoxia on starch breakdown in rice and wheat seeds
P. Perata (2004)
10.1104/PP.126.2.742
Signaling events in the hypoxic induction of alcohol dehydrogenase gene in Arabidopsis.
H. Peng (2001)
10.1093/OXFORDJOURNALS.AOB.A010302
Strategies of Gene Action in Arabidopsis during Hypoxia
R. Dolferus (1997)
10.17660/ACTAHORTIC.1987.212.26
" VITRIFICATION " : REVIEW OF LITERATURE
M. Pâques (1987)
Interaction between sucrose uptake and photosynthesis in micropropagated Rosa multiflora L.
J. Riek (1995)
10.1023/B:TICU.0000016825.18930.e4
Hyperhydricity of Micropropagated Shoots: A Typically Stress-induced Change of Physiological State
C. Kevers (2004)
10.1016/s0021-9258(19)52451-6
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
10.1002/CBF.290040403
Physiological functions of the pentose phosphate pathway
T. Wood (1986)
10.1007/BF00033546
Cell wall invertase activity in cultured tobacco tissues
H. Obata-Sasamoto (2004)
10.1007/BF00034307
Reconsideration of the term ‘vitrification’ as used in micropropagation
P. Debergh (2004)
Responses to Abiotic Stress
J. Zhu (2002)
10.1016/J.PLAPHY.2004.05.003
Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response.
T. Franck (2004)
10.1093/JEXBOT/52.359.1179
Anoxic stress leads to hydrogen peroxide formation in plant cells.
O. Blokhina (2001)
10.1007/BF00232226
Environmental scanning electron microscopy of the surface of normal and vitrified leaves of Gypsophila paniculata (Babies Breath) cultured in vitro
Karleen Gribble (2004)
10.1104/PP.100.1.1
Anaerobic metabolism in plants.
R. A. Kennedy (1992)
10.1023/A:1015173724712
Plant Pyruvate Kinase
P. K. Ambasht (2004)
10.1111/j.1399-3054.1981.tb04130.x
Mass propagation of globe artichoke (Cynara scolymus): Evaluation of different hypotheses to overcome vitrification with special reference to water potential
P. Debergh (1981)
10.1006/ABIO.1997.2283
Cycling assay for nicotinamide adenine dinucleotides: NaCl precipitation and ethanol solubilization of the reduced tetrazolium.
Y. Gibon (1997)
10.1111/J.1399-3054.1984.TB06102.X
Physiological and biochemical events leading to vitrification of plants cultured in vitro
C. Kevers (1984)
10.1104/PP.100.2.820
Normal growth of transgenic tobacco plants in the absence of cytosolic pyruvate kinase.
S. Gottlob-McHugh (1992)
Vitrification of carnation in vitro: changes in water content, extracellular space, air volume, and ion levels
C. Kevers (1986)
10.1016/S0176-1617(98)80248-4
Identification of pinitol as a main sugar constituent and changes in its content during flower bud development in carnation (Dianthus caryophyllus L.)
K. Ichimura (1998)



This paper is referenced by
10.1007/s00344-008-9072-5
Growth Conditions in In Vitro Culture Can Induce Oxidative Stress in Mammillaria gracilis Tissues
B. Balen (2008)
10.1016/J.ENVEXPBOT.2007.08.004
Sub-cellular location of H2O2, peroxidases and pectin epitopes in control and hyperhydric shoots of carnation
N. Fernández-García (2008)
The hyperhydricity syndrome: waterlogging of plant tissues as a major cause
L. Martinez (2010)
10.1007/s11816-009-0124-5
Shoot multiplication kinetics and hyperhydric status of regenerated shoots of gladiolus in agar-solidified and matrix-supported liquid cultures
S. Dutta Gupta (2009)
10.1007/s10725-012-9668-4
Response of antioxidant systems in oxygen deprived suspension cultures of rice (Oryza sativa L.)
R. I. Damanik (2012)
10.1016/j.ecoenv.2010.01.005
Cadmium impairs mineral and carbohydrate mobilization during the germination of bean seeds.
Amira Sfaxi-Bousbih (2010)
THE EFFECT OF HEAT STRESS ON HYPERHYDRICITY AND GUAIACOL PEROXIDASE ACTIVITY ( GPOX ) AT THE FOLIAR LAMINA OF SEDUM TELEPHIUM L . SSP . MAXIMUM ( L . ) KROCK . VITROPLANTLETS
M. Ardelean (2015)
10.1007/s12298-010-0033-7
NAD pattern and NADH oxidase activity in pea (Pisum sativum L.) under cadmium toxicity
M. Smiri (2010)
10.1007/s11240-011-9940-8
Promoting root induction and growth of in vitro macadamia (Macadamia tetraphylla L. ‘Keaau’) plantlets using CO2-enriched photoautotrophic conditions
S. Cha-um (2011)
10.1590/S0103-84782012000500013
Hiperidricidade: uma desordem metabólica
Andréa Guimarães Vieira de Vasconcelos (2012)
10.1016/J.SCIENTA.2018.07.021
Aeration of different irrigation levels affects net global warming potential and carbon footprint for greenhouse tomato systems
H. Chen (2018)
Towards automating micropropagation: from cells to shoots to plants in one step
Liwen Fei (2015)
10.1186/1471-2229-6-25
Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes
Yuanqing Jiang (2006)
10.1007/s10725-007-9185-z
Reduction of hyperhydricity in the culture of Lepidium meyenii shoots by the addition of rare earth elements
Ya-li Wang (2007)
A protocol for the mass-micropropagation of carnation (Dianthus caryophyllus L.)
S. Arabia (2011)
10.2477/VOL14ISS1PP1-7
Micropropagación de Achyrocline flaccida (Weinm.) DC. en medios de cultivo líquidos
S. Ross (2010)
10.1201/9781439854082-13
Metal Toxicity, Oxidative Stress and Antioxidative Defense System in Plants
Nieves Fernández-García (2010)
10.1016/J.SCIENTA.2006.01.038
Morpho-physiological disorders in in vitro culture of plants
B. N. Hazarika (2006)
10.1201/B17684-14
Global warming impact on rice crop productivity
R. S. Sengar (2014)
10.1016/J.PLANTSCI.2007.04.012
Transcriptional profiling of canola (Brassica napus L.) responses to the fungal pathogen Sclerotinia sclerotiorum
Bo Yang (2007)
10.3390/ijms10083583
Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity
Veronika Doubnerová (2009)
10.1016/J.SCIENTA.2017.02.010
Effects of multiple factors on hyperhydricity of Allium sativum L.
Liu Min (2017)
10.1139/CJB-2018-0041
Salicylic acid mitigates hyperhydricity in newly developed potato shoots through reduced oxidation
Ma Zhi-gang (2018)
10.1016/j.plantsci.2010.12.005
What can enzymes of C₄ photosynthesis do for C₃ plants under stress?
V. Doubnerová (2011)
In Vitro Liquid Culture Systems of Scutellaria Species
A. Tascan (2007)
10.1007/978-981-10-7434-9_4
Plant Biosynthetic Engineering Through Transcription Regulation: An Insight into Molecular Mechanisms During Environmental Stress
R. Srivastava (2018)
10.1007/s00726-008-0039-4
Cold stress changes the concanavalin A-positive glycosylation pattern of proteins expressed in the basal parts of rice leaf sheaths
S. Komatsu (2008)
Physiological responses of rice cultivars exposed to different temperatures and flood depths in a water seeded system
Gerson Meneghetti Sarzi Sartori (2015)
10.1007/s11627-019-10038-y
Evaluating a DOE screen to reduce hyperhydricity in the threatened plant, Cycladenia humilis var. jonesii
V. Pence (2020)
10.1007/s10535-008-0085-5
Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer
P. Báťková (2008)
Micropropagation of Achyrocline flaccida (Weinm.) DC. in liquid culture media.
S. Ross (2010)
10.1016/J.SCIENTA.2008.07.010
Biochemical markers of morphogenesis in long term horseradish (Armoracia lapathifolia Gilib.) tissue culture
B. Balen (2009)
See more
Semantic Scholar Logo Some data provided by SemanticScholar