Online citations, reference lists, and bibliographies.
← Back to Search

Molecular Characterization Of Chromium (VI) Reducing Potential In Gram Positive Bacteria Isolated From Contaminated Sites

R. C. Patra, R. C. Patra, Seidu Malik, M. Beer, M. Megharaj, R. Naidu
Published 2010 · Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
Hexavalent chromium [Cr(VI)] is highly toxic, teratogenic and carcinogenic to man and other animals. Some bacterial species have the ability to reduce Cr(VI) to a stable speciation state of trivalent chromium [Cr(III)], which is insoluble and comparatively less toxic. Therefore, the reduction of Cr(VI) thus provides potential as a means for environmental bioremediation of Cr(VI) pollution. In the present study bacteria isolated from chromium and diesel contaminated sites were found to have the ability to rapidly reduce highly toxic concentrations of Cr(VI) to Cr(III) when grown in minimal medium supplemented with glucose as the sole carbon source. Partial chromate reductase gene sequences were retrieved after PCR amplification of genomic DNA extracted from three Gram positive isolates which were highly similar (>99% sequence similarity) to chromate reductase genes found in Gram negative bacteria, more specifically those identified from Escherichia coli and Shigella spp. whole-genome studies. The isolated bacteria were putatively identified by 16S rRNA gene sequencing as Arthrobacter aurescens strain MM10, Bacillus atrophaeus strain MM20, and Rhodococcus erythropolis strain MM30.
This paper references
10.1016/J.ENVINT.2003.08.001
Metal bioremediation through growing cells.
Anushree Malik (2004)
10.1146/ANNUREV.MI.47.100193.001403
Dissimilatory metal reduction.
D. Lovley (1993)
Methods for general and molecular bacteriology
P. Gerhardt (1994)
10.1016/S0168-6445(03)00048-2
Efflux-mediated heavy metal resistance in prokaryotes.
D. Nies (2003)
Nucleotide sequence and expression of a plasmid-encoded chromate resistance determinant from Alcaligenes eutrophus.
A. Nies (1990)
10.1128/AEM.69.8.4390-4395.2003
Vibrio harveyi Nitroreductase Is Also a Chromate Reductase
Young Hak Kwak (2003)
10.1016/J.CHEMOSPHERE.2006.12.051
Heavy metal resistance and genotypic analysis of metal resistance genes in gram-positive and gram-negative bacteria present in Ni-rich serpentine soil and in the rhizosphere of Alyssum murale.
R. A. I. Abou-Shanab (2007)
10.1016/J.EJSOBI.2009.06.009
Bioreduction of chromium (VI) by Bacillus sp. isolated from soils of iron mineral area
Guojun Cheng (2009)
10.1128/JB.174.16.5340-5345.1992
NAD(P)H-dependent chromium (VI) reductase of Pseudomonas ambigua G-1: a Cr(V) intermediate is formed during the reduction of Cr(VI) to Cr(III).
T. Suzuki (1992)
10.1007/s10482-008-9247-x
Genes related to chromate resistance by Pseudomonas aeruginosa PAO1
S. L. Rivera (2008)
10.1016/S0006-291X(02)00438-2
A bacterial flavin reductase system reduces chromate to a soluble chromium(III)-NAD(+) complex.
G. Puzon (2002)
10.1016/J.JHAZMAT.2005.01.018
Bioremediation of Cr(VI) in contaminated soils.
K. R. Krishna (2005)
10.1007/s00284-009-9478-8
Kinetic Study and Mathematical Modeling of Chromium(VI) Reduction and Microorganism Growth Under Mixed Culture
B. Kong (2009)
10.1128/AEM.66.5.1788-1795.2000
Purification to Homogeneity and Characterization of a Novel Pseudomonas putida Chromate Reductase
C. H. Park (2000)
10.1128/JB.01766-07
A novel chromate reductase from Thermus scotoductus SA-01 related to old yellow enzyme.
D. Opperman (2008)
10.2134/JEQ2003.1228
Chromate reduction by chromium-resistant bacteria isolated from soils contaminated with dichromate.
F. A. O. Camargo (2003)
10.1016/S0378-1097(04)00068-0
Essential residues in the chromate transporter ChrA of Pseudomonas aeruginosa.
S. Aguilera (2004)
10.1007/s00284-002-3889-0
Toxicity of Hexavalent Chromium and Its Reduction by Bacteria Isolated from Soil Contaminated with Tannery Waste
M. Megharaj (2003)
16S/23S rRNA sequencing
D. J. Lane (1991)
10.1007/0-387-21728-2_3
Chemistry of chromium in soils with emphasis on tannery waste sites.
S. Avudainayagam (2003)
10.2134/JEQ1979.00472425000800010008X
Behavior of chromium in soils. III. Oxidation
R. J. Bartlett (1979)
10.1023/A:1017974415052
Gene expression profile in response to chromium-induced cell stress in A549 cells
J. Ye (2004)
10.1016/J.IBIOD.2006.05.002
Mechanism of hexavalent chromium detoxification by microorganisms and bioremediation application potential: A review
K. H. Cheung (2007)
Chromate Resistance and Reduction by Bacterial Isolates
E. Parameswari (2009)
10.1002/BIT.10261
Chromate/nitrite interactions in Shewanella oneidensis MR-1: evidence for multiple hexavalent chromium [Cr(VI)] reduction mechanisms dependent on physiological growth conditions.
S. Viamajala (2002)
10.1016/J.BIORTECH.2006.06.011
Reduction of chromate by cell-free extract of Brucella sp. isolated from Cr(VI) contaminated sites.
Urvashi Thacker (2007)
10.1128/AEM.00347-09
Inhibition of Nitrate Reduction by Chromium(VI) in Anaerobic Soil Microcosms
P. Kourtev (2009)
10.1111/J.1574-6976.2001.TB00581.X
Interactions of chromium with microorganisms and plants.
C. Cervantes (2001)
Prominent role of DT-diaphorase as a cellular mechanism reducing chromium(VI) and reverting its mutagenicity.
S. De Flora (1985)
10.1128/JB.181.23.7398-7400.1999
Chromate efflux by means of the ChrA chromate resistance protein from Pseudomonas aeruginosa.
A. H. Álvarez (1999)
10.1007/s00253-003-1390-8
Hydrogenases in sulfate-reducing bacteria function as chromium reductase
B. Chardin (2003)
10.1093/BIB/5.2.150
MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment
S. Kumar (2004)
Nucleic acid techniques in bacterial systematics
E. Stackebrandt (1991)
10.1186/1471-2180-6-5
Low temperature reduction of hexavalent chromium by a microbial enrichment consortium and a novel strain of Arthrobacter aurescens
Rene' N Horton (2005)
10.1007/s00284-005-0048-4
Reduction of Hexavalent Chromium by Cell-Free Extract of Bacillus sphaericus AND 303 Isolated from Serpentine Soil
Arundhati Pal (2005)
10.1093/CARCIN/12.5.825
Reductive metabolism and protein binding of chromium(VI) by P450 protein enzymes.
A. Mikalsen (1991)
10.1016/0147-619X(92)90007-W
Plasmid chromate resistance and chromate reduction.
C. Cervantes (1992)
10.1128/AEM.03006-05
Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
T. Z. Desantis (2006)
10.1016/j.biortech.2007.12.046
Evaluation of in vitro Cr(VI) reduction potential in cytosolic extracts of three indigenous Bacillus sp. isolated from Cr(VI) polluted industrial landfill.
Chirayu Desai (2008)
10.1093/nar/gkl244
NAST: a multiple sequence alignment server for comparative analysis of 16S rRNA genes
T. DeSantis (2006)
10.1007/BF00130763
Microbiological process for the removal of Cr(VI) from chromate-bearing cooling tower effluent
J. Bhide (2004)
10.1007/BF00128577
Plasmid mediated chromate resistance and reduction in Pseudomonas mendocina MCM B-180
P. Dhakephalkar (2004)
10.3892/OR.5.6.1307
Chromium-induced genotoxicity and apoptosis: relationship to chromium carcinogenesis (review).
J. Singh (1998)



This paper is referenced by
Estrategias bacterianas de supervivencia como herramientas en Biotecnología Ambiental
Irene Behrmann (2015)
10.1007/s11356-018-1764-1
Identification of the main mechanisms involved in the tolerance and bioremediation of Cr(VI) by Bacillus sp. SFC 500-1E
Ornella M. Ontañon (2018)
STRUCTURAL, FUNCTIONAL AND EVOLUTIONARY ANALYSIS OF CHROMATE REDUCTASES
Aparna Satapathy (2013)
10.1016/j.ram.2016.04.001
Economical fermentation media for the production of a whole cell catalyst for the treatment of Cr(VI)-containing wastewaters.
M. J. Alessandrello (2016)
10.2166/wst.2019.060
Alleviation of hexavalent chromium by using microorganisms: insight into the strategies and complications.
A. Bhattacharya (2019)
10.4028/www.scientific.net/AMR.610-613.1789
Promoting Influence of Organic Carbon Source on Chromate Reduction by Bacillus sp.
W. H. Xu (2012)
10.1016/J.JKSUS.2018.05.001
Isolation and identification of chromium reducing bacteria from tannery effluent
M. Sanjay (2020)
10.3390/molecules23020406
Reduction of Hexavalent Chromium and Detection of Chromate Reductase (ChrR) in Stenotrophomonas maltophilia
R. Baldiris (2018)
10.1007/s11356-012-1178-4
In vitro Cr(VI) reduction by cell-free extracts of chromate-reducing bacteria isolated from tannery effluent irrigated soil
S. Soni (2012)
10.1002/9781118371220.CH7
Biosorption of Heavy Metals – New Perspectives
T. Tavares (2012)
10.1007/978-3-030-04477-0_8
Polymer-Based Magnetic Nanocomposites for the Removal of Highly Toxic Hexavalent Chromium from Aqueous Solutions
M. J. Hato (2019)
10.1016/j.jhazmat.2012.04.054
A review of chemical, electrochemical and biological methods for aqueous Cr(VI) reduction.
C. Barrera-Díaz (2012)
10.1007/s00128-019-02683-1
Prevention of Oxidative Damage and Phytoremediation of Cr(VI) by Chromium(VI) Reducing Bacillus subtilus PAW3 in Cowpea Plants
P. A. Wani (2019)
10.1007/978-981-10-7284-0_5
Heavy Metal Contamination: An Alarming Threat to Environment and Human Health
Sandhya Mishra (2019)
10.3967/bes2015.014
Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China.
R. Zhao (2016)
10.1007/s10532-018-9856-7
Simultaneous removal of hexavalent chromium and o-dichlorobenzene by isolated Serratia marcescens ZD-9
W. Xu (2018)
10.1007/s11356-015-4571-y
Biochemical and molecular mechanisms involved in simultaneous phenol and Cr(VI) removal by Acinetobacter guillouiae SFC 500-1A
O. Ontañon (2015)
10.1007/s00792-015-0733-6
Bioreduction and immobilization of hexavalent chromium by the extremely acidophilic Fe(III)-reducing bacterium Acidocella aromatica strain PFBC
Yusei Masaki (2015)
10.1016/j.envint.2019.104926
Microbial chromate reduction coupled with anaerobic oxidation of methane in a membrane biofilm reactor.
Jing-Huan Luo (2019)
10.2343/GEOCHEMJ.2.0278
Cr(VI) removal by biogenic schwertmannite in continuous flow column
C. Yu (2014)
10.3390/ijerph17030704
Enhancement of Chromium (VI) Reduction in Microcosms Amended with Lactate or Yeast Extract: A Laboratory-Scale Study
V. Ancona (2020)
10.1016/J.IBIOD.2013.10.017
Rhizoremediation of phenol and chromium by the synergistic combination of a native bacterial strain and Brassica napus hairy roots
O. Ontañon (2014)
10.1007/s00244-014-0003-0
Pretreatment of Cr(VI)-Amended Soil With Chromate-Reducing Rhizobacteria Decreases Plant Toxicity and Increases the Yield of Pisum sativum
S. Soni (2014)
10.5539/IJB.V6N2P64
Enzymatic Chromium (VI) Reduction by Cytoplasmic and Cell Membrane Fractions of Chromate-Reducing Bacterium Isolated From Sewage Treatment Plant
Paul Fabrice Nguema (2014)
Conversion of toxic hexavalent chromium to trivalent chromium by rhamnolipid stabilized zero valent iron nanoparticles
Fatima Nasser (2013)
10.1007/978-3-319-10479-9_2
Mechanisms of hexavalent chromium resistance and removal by microorganisms.
N. T. Joutey (2015)
10.1080/02757540.2020.1799992
Biotransformation of chromium (VI) by Bacillus sp. isolated from chromate contaminated landfill site
M. E. Karim (2020)
acterial diversity in the Cr ( VI ) reducing biocathode of Microbial Fuel Cell with salt bridge olly
Margot Revelo Romoa (2019)
10.1007/S11771-013-1509-8
Optimization of Cr(VI) bioremediation in contaminated soil using indigenous bacteria
Q. Li (2013)
10.1016/j.jenvman.2014.07.014
Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium: a review.
H. Thatoi (2014)
10.1007/s11274-019-2638-5
Chromate tolerance and removal of bacterial strains isolated from uncontaminated and chromium-polluted environments
Dragana Tamindžija (2019)
10.4018/978-1-7998-4888-2.ch005
Role of Bacterial Chromate Reductase in Bioremediation of Chromium-Containing Wastes
Satarupa Dey (2021)
See more
Semantic Scholar Logo Some data provided by SemanticScholar