Online citations, reference lists, and bibliographies.
← Back to Search

Planar Waveguides For Ultra-high Sensitivity Of The Analysis Of Nucleic Acids

G. Duveneck, A. Abel, M. Bopp, G. M. Kresbach, M. Ehrat
Published 2002 · Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Abstract In the first part of this paper, the need for analytical techniques capable of highly parallel and sensitive nucleic acid analysis, with the capability of achieving very low limits of detection (LODs) and of resolving small differences in concentration, is described. Whereas the requirement for performing simultaneously multi-analyte detection is solved by the approach of nucleic acid microarrays, requirements on sensitivity can often not be satisfied by classical detection technologies. Inherent limitations of conventional fluorescence excitation and detection schemes are identified, and the implementation of planar waveguides as analytical platforms for nucleic acid microarrays, with fluorescence excitation in the evanescent field associated with the guided excitation light, is proposed. The relevant parameters for an optimization of sensitivity are discussed. In the second part of this paper, the specific formats of our planar waveguide platforms, which are compatible with established industrial standard formats allowing for integration into industrial high throughput environments, are presented, as well as the dedicated optical system for fluorescence excitation and detection that we developed. In a direct comparison with a state-of-the-art scanner, it is demonstrated that the implementation of genomic microarrays on planar waveguide platforms allows for unprecedented, direct detection of low-abundant genes in limited amounts of sample. Otherwise, when using conventional fluorescence excitation and detection configurations, the detection of such low amounts of nucleic acids requires massive sample preparation and signal or target amplification steps.
This paper references
Reduction of fibrous adhesion formation by a copolymer possessing an affinity for anionic surfaces.
D. Elbert (1998)
The development of microfabricated arrays for DNA sequencing and analysis.
M. J. O'Donnell-Maloney (1996)
Parallel synthesis and analysis of large numbers of related chemical compounds: applications to oligonucleotides.
E. Southern (1994)
Theory of Prism–Film Coupler and Thin-Film Light Guides
P. Tien (1970)
Absorption and Emission of Evanescent Photons
C. Carniglia (1972)
DNA chips: analysing sequence by hybridization to oligonucleotides on a large scale.
E. Southern (1996)
An Array Immunosensor for Simultaneous Detection of Clinical Analytes
Chris A. Rowe (1999)
Multiple internal reflection fluorescence spectrometry
N. Harrick (1973)
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
M. Schena (1995)
Gene expression profiles of laser-captured adjacent neuronal subtypes
L. Luo (1999)
Total Internal Reflection Fluorescence Surface Sensors
J. T. Ives (1987)
Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two–colour fluorescence analysis
J. Hacia (1996)
Light-directed, spatially addressable parallel chemical synthesis.
S. P. Fodor (1991)
Direct cloning and sequence analysis of enzymatically amplified genomic sequences.
S. Scharf (1986)

This paper is referenced by
Application of total internal reflexion fluorescence microscopy for studying pH changes in an occluded electrochemical cell: Development of a waveguide sensor
F. Loete (2006)
A high-sensitivity, medium-density, and target amplification-free planar waveguide microarray system for gene expression analysis of formalin-fixed and paraffin-embedded tissue.
S. Schwers (2009)
Sol–gel planar waveguides for improved fluorescence microarrays
Mélanie Bedu (2010)
Waveguide excitation fluorescence microscopy: a new tool for sensing and imaging the biointerface.
H. M. Grandin (2006)
Direct light-coupling to thin-film waveguides using a grating-structured GRIN lens.
T. Fricke-Begemann (2010)
Total internal reflection fluorescence (TIRF) array biosensors for biothreat agents for food safety and food defense
Chris Rowe Taitt (2015)
High-reflectivity Bragg gratings fabricated by 248-nm excimer laser holographic ablation in thin Ta2O5 films overlaid on glass waveguides
S. Pissadakis (2004)
Quantification of E. coli DNA on a flow-through chemiluminescence microarray readout system after PCR amplification.
S. Donhauser (2009)
Comparison of the chondrosarcoma cell line SW1353 with primary human adult articular chondrocytes with regard to their gene expression profile and reactivity to IL-1beta.
M. Gebauer (2005)
SECONDA UNIVERSITÀ DEGLI STUDI DI NAPOLI Facoltà di Ingegneria Dottorato di Ricerca in Ingegneria Elettronica
L. Zeni (2005)
Perfluorinated polymer optical fiber tapers for fluorescence collection
R. Gravina (2009)
Conception et élaboration d'un capteur optique à ondes guidées pour la détection d'espèces chimiques.
T. Mazingue (2005)
Total Internal Reflection Fluorescence Quantification of Receptor Pharmacology
Ye Fang (2015)
Fabrication and optimization of Tantalum pentoxide waveguides for optical micro-propulsion
Balpreet Singh Ahluwalia (2010)
An integrated multimodal acoustic particle manipulator and optical waveguide sensor
P. Glynne-Jones (2008)
Flexible acoustic particle manipulation device with integrated optical waveguide for enhanced microbead assays.
P. Glynne-Jones (2009)
Quantitative analysis of enhanced light irradiance in waveguide-based fluorescent microarrays.
Gabriel Sagarzazu (2009)
Integrated optical fluorescence multi-sensor system
P. Hua (2009)
Fluorescence-based Biosensors
Michael Schäferling (2011)
Mesoporous Thin-film Materials Studied by Optical Waveguide Spectroscopy
Antun Peic (2009)
Performance of integrated optical microcavities for refractive index and fluorescence sensing
E. Krioukov (2003)
A regenerable immunochip for the rapid determination of 13 different antibiotics in raw milk.
K. Kloth (2009)
Detection of lipopolysaccharides in serum using a waveguide-based optical biosensor
Aneesa Noormohamed (2017)
Fabrication of a cyclic olefin copolymer planar waveguide embedded in a multi-channel poly(methyl methacrylate) fluidic chip for evanescence excitation.
Paul I. Okagbare (2010)
Optical Chemical Sensors: A Look Back
D. Wencel (2012)
Evanescent field Sensors Based on Tantalum Pentoxide Waveguides – A Review
K. Schmitt (2008)
Fluorescent vesicles for signal amplification in reverse phase protein microarray assays.
Marta Bally (2011)
Effective transcriptome amplification for expression profiling on sense-oriented oligonucleotide microarrays
Joerg Schlingemann (2005)
Development of a non-labeled immunosensor for the herbicide trifluralin via optical waveguide lightmode spectroscopic detection
A. Székács (2003)
Réalisation de guides d'onde plans faibles pertes en nitrure de silicium pour un biocapteur intégré
A. Gorin (2009)
High-index contrast waveguides for microparticle guidance
B. Ahluwalia (2008)
Fabrication of silicon and glass devices for microfluidic bioanalytical applications
K. Kolari (2008)
See more
Semantic Scholar Logo Some data provided by SemanticScholar