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Simple Method For The Determination Of Benzodiazepines In Human Body Fluids By High-performance Liquid Chromatography–mass Spectrometry

X. Lee, T. Kumazawa, J. Sato, Yukiko Shoji, Chika Hasegawa, C. Karibe, Tetsuya Arinobu, H. Seno, Keizo Sato
Published 2003 · Chemistry
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Abstract Three benzodiazepines, etizolam, brotizolam and lorazepam, have been analyzed from human plasma and urine samples by high-performance liquid chromatography–mass spectrometry (HPLC–MS) with a new polymer column (MSpak GF), which enabled direct injection of crude biological samples. The recoveries of the three benzodiazepines spiked into plasma and urine were 81.7–90.7 and 78.7–91.5%, respectively. The regression equations for the three benzodiazepines showed excellent linearity in the range 10–500 ng ml −1 of plasma or urine. The detection limits were 2 ng ml −1 for etizolam and brotizolam, 5 ng ml −1 for lorazepam in both samples. The intra- and inter-day precisions for plasma and urine were not greater than 12.7%. The data obtained from determination of the benzodiazepines in rat plasma after oral administration of the drugs are also presented.



This paper is referenced by
10.1007/s11419-006-0002-6
Determination of some antiallergic drugs in human plasma by direct-injection high-performance liquid chromatography-tandem mass spectrometry
Koichiro Fujimaki (2006)
10.1080/10826076.2012.678459
A RAPID HILIC METHOD FOR ANALYSIS OF DIAZEPAM AND ESTAZOLAM IN HUMAN PLASMA
Qin Yuan (2012)
10.1016/j.aca.2014.06.007
Dispersive micro-solid-phase extraction of benzodiazepines from biological fluids based on polyaniline/magnetic nanoparticles composite.
Ali Akbar Asgharinezhad (2014)
10.1016/J.MSEC.2011.02.013
Spectrophotometric and chemometric studies on the simultaneous determination of two benzodiazepines in human plasma
Siavash Riahi (2011)
10.4155/bio.09.43
Benzodiazepines: sample preparation and HPLC methods for their determination in biological samples.
V. Samanidou (2009)
10.1016/j.aca.2010.08.019
Rapid ultraviolet monitoring of multiple psychotropic drugs with a renewable microfluidic device.
Jin Sheng (2010)
10.1007/s11419-008-0058-6
High-throughput determination of theophylline and caffeine in human serum by conventional liquid chromatography-mass spectrometry
Tetsuya Arinobu (2008)
10.1080/10826070802019574
Development and Validation of an HPLC Method for the Determination of Six 1,4‐Benzodiazepines in Pharmaceuticals and Human Biological Fluids
M. N. Uddin (2008)
10.4103/0250-474X.58194
Simultaneous HPLC Determination of Butenafine Hydrochloride and Betamethasone in a Cream Formulation
Rajesh Ankam (2009)
10.1002/jssc.200800342
Validation of SPE-HPLC determination of 1,4-benzodiazepines and metabolites in blood plasma, urine, and saliva.
M. N. Uddin (2008)
10.4155/bio.11.46
Quantification of total and unbound concentrations of lorazepam, oxazepam and temazepam in human plasma by ultrafiltration and LC-MS/MS.
Helle S Larsen (2011)
10.1007/s11419-010-0096-8
Determination of nonsteroidal anti-inflammatory drugs in human plasma by LC-MS-MS with a hydrophilic polymer column
Xiao-Pen Lee (2010)
10.2478/s11532-014-0551-1
Analytical methods for determination of benzodiazepines. A short review
Paulina Szatkowska (2014)
10.1080/03067310802538493
Gold nanoparticle-based immunochromatographic assay for the detection of 7-aminoclonazepam in urine
Qiusheng Li (2009)
10.1007/s00216-010-3572-4
Experimental design for optimization of microwave-assisted extraction of benzodiazepines in human plasma
Purificación Fernández (2010)
METHOD DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ESCITALOPRAM OXALATE AND ETIZOLAM IN BULK AND ITS PHARMACEUTICAL FORMULATIONS
Kalpana Nekkala (2014)
10.1080/00032710600622126
Determination of Trace Amounts of Lorazepam by Adsorptive Cathodic Differential Pulse Stripping Method in Pharmaceutical Formulations and Biological Fluids
Jahan B. Ghasemi (2006)
Development of validated analytical methods for some drugs and their formulations
Suryakant D Bhosale (2011)
10.1007/s11419-007-0020-z
An automated on-line method for simultaneous analysis of phenothiazines in human serum by high-performance liquid chromatography/sonic spray ionization mass spectrometry using backflush column switching
R. M. Aoki (2007)
Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Escitalopram oxalate and Etizolam in their Combined Tablet Dosage Form
Bhumika. D. Sakhreliya (2012)
10.1016/j.talanta.2008.11.003
Simultaneous determination of multibenzodiazepines by HPLC/UV: investigation of liquid-liquid and solid-phase extractions in human plasma.
Keyller Bastos Borges (2009)
10.1002/bmc.1566
LC-MS in analytical toxicology: some practical considerations.
Lewis Couchman (2011)
10.1007/s00604-012-0897-z
Sensing Lorazepam with a glassy carbon electrode coated with an electropolymerized-imprinted polymer modified with multiwalled carbon nanotubes and gold nanoparticles
B. Rezaei (2012)
10.1016/j.jchromb.2016.08.047
Development and application of a multi-component LC-MS/MS method for determination of designer benzodiazepines in urine.
Madeleine Pettersson Bergstrand (2016)
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