Online citations, reference lists, and bibliographies.
Referencing for people who value simplicity, privacy, and speed.
Get Citationsy
← Back to Search

Inhibition Of O2-reducing Activity Of Horseradish Peroxidase By Diphenyleneiodonium.

G. Frahry, P. Schopfer
Published 1998 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Plant cells respond to pathogen attach with a burst of H2O2 secretion. The question whether this defense reaction is catalysed by a NAD(P)H oxidase similar to the NADPH oxidase of phagocytic leukocytes in mammals or by an extracellular peroxidase is presently a matter of controversial debate. The observation that H2O2 production by plant cells can be inhibited by diphenyleneiodonium (DPI), a potent inhibitor of the mammalian NADPH oxidase, has fostered the view that a mammalian-type enzyme is responsible for the H2O2 production by plant cells. Here we show that DPI inhibits the NADH-dependent H2O2 production by horseradish peroxidase in the same concentration range as previously used for the inhibition of putative NADPH oxidase activity in plants. The peroxidative activity normally used for assaying peroxidase is not affected by DPI, indicating that the inhibitor specifically interferes with a partial reaction that is exclusively involved in the O2 reducing activity of the enzyme.
This paper references
Free radicals in biology and medicine
B. Halliwell (1985)
The origin of the oxidative burst in plants.
G. Bolwell (1996)
Methods of Enzymology.
H. Tauber (1956)
Further studies on the formation of oxygen radicals by potassium superoxide in aqueous medium for biochemical investigations.
B. Lokesh (1986)
Plant and human neutrophil oxidative burst complexes contain immunologically related proteins.
S. C. Dwyer (1996)
Effects of fatty acids on superoxide radical generation in leukocytes.
K. Kakinuma (1978)
Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf disks
M. Peng (1992)
The effect of the NADPH oxidase inhibitor diphenyleneiodonium on aerobic and anaerobic microbicidal activities of human neutrophils.
J. Ellis (1988)
A Sensitive Method for the Estimation of Hydrogen Peroxide in Biological Materials
W. Andreae (1955)
Production of superoxide radicals in glyoxysomal membranes from castor bean endosperm
L. A. Río (1995)
The biochemical basis of the NADPH oxidase of phagocytes.
A. Segal (1993)
Réactions des dérivés de l'iode(III) avec les ferroporphyrines et le cytochrome P-450: formation de complexes σ-aryles du fer(III) et de N-aryl-porphyrines du fer(II) à partir de sels de diaryliodonium
Jean-Paul Battioni (1988)
Initiation of Runaway Cell Death in an Arabidopsis Mutant by Extracellular Superoxide
T. Jabs (1996)
H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response
Alex Levine (1994)
The origin of the oxidative burst in plants.
G. Bolwell (1995)
Diphenylene iodonium as an inhibitor of the NADPH oxidase complex of bovine neutrophils. Factors controlling the inhibitory potency of diphenylene iodonium in a cell-free system of oxidase activation.
J. Doussière (1992)
Enzymic mechanisms of superoxide production.
A. Cross (1991)
NAD(P)H oxidase and peroxidase activities in purified plasma membranes from cauliflower inflorescences
P. Askerlund (1987)
Studies on the inhibitory mechanism of iodonium compounds with special reference to neutrophil NADPH oxidase.
B. O’Donnell (1993)
The use of acetylated ferricytochrome c for the detection of superoxide radicals produced in biological membranes.
A. Azzi (1975)
The effect of the inhibitor diphenylene iodonium on the superoxide-generating system of neutrophils. Specific labelling of a component polypeptide of the oxidase.
A. Cross (1986)
Generation of active oxygen in elicited cells of Arabidopsis thaliana is mediated by a NADPH oxidase‐like enzyme
R. Desikan (1996)
Histochemical Evidence of Polyamine Oxidation and Generation of Hydrogen Peroxide in the Cell Wall
R. Angelini (1989)
Plasma Membrane Redox Enzyme Is Involved in the Synthesis of O2- and H2O2 by Phytophthora Elicitor-Stimulated Rose Cells
C. Auh (1995)
Involvement of an NAD(P)H oxidase in the elicitor-inducible oxidative burst of soybean
A. Mithöfer (1997)
The Ca2+/NADPH-dependent H2O2 generator in thyroid plasma membrane: inhibition by diphenyleneiodonium.
D. Deme (1994)

This paper is referenced by
Reactive Oxygen Species in the Elongation Zone of Maize Leaves Are Necessary for Leaf Extension1
A. A. Rodríguez (2002)
A proposed interplay between peroxidase, amine oxidase and lipoxygenase in the wounding-induced oxidative burst in Pisum sativum seedlings.
T. Roach (2015)
Oxygen metabolism in plant/bacteria interactions: effect of DPI on the pseudo-NAD(P)H oxidase activity of peroxidase.
C. J. Baker (1998)
Production of Reactive Oxygen Intermediates (O2˙−, H2O2, and ˙OH) by Maize Roots and Their Role in Wall Loosening and Elongation Growth
A. Liszkay (2004)
Reactive oxygen species metabolism in desiccation‐stressed thalli of the liverwort Dumortiera hirsuta
R. Beckett (2004)
NADPH oxidase-dependent reactive oxygen species
M. Jones (2007)
Reactive Oxygen Species in Cell Walls
R. Vreeburg (2007)
Redox-related peroxidative responses evoked by methyl-jasmonate in axenically cultured aeroponic sunflower (Helianthus annuus L.) seedling roots
I. Garrido (2003)
Generation of the oxidative burst - scavenging for the truth.
Bestwick (1999)
Tissue-specific expression of a defence-related peroxidase in transgenic wheat potentiates cell death in pathogen-attacked leaf epidermis.
P. Schweizer (2008)
Reactive oxygen species: metabolism, oxidative stress, and signal transduction.
K. Apel (2004)
Reactive Oxygen Species Formation and Cell Death in Catalase-Deficient Tobacco Leaf Discs Exposed to Paraquat
M. Iannone (2011)
Temporal-Spatial Interaction between Reactive Oxygen Species and Abscisic Acid Regulates Rapid Systemic Acclimation in Plants[W][OPEN]
N. Suzuki (2013)
Kinetic study of acrylamide radical polymerization initiated by the horseradish peroxidase–mediated system
Zhiqi Cai (2012)
Superoxide and its metabolism during germination and axis growth of Vigna radiata (L.) Wilczek seeds
K. L. Singh (2014)
An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes
D. M. Mojovic (2005)
Reactive oxygen species in programmed death of pea guard cells
V. Samuilov (2008)
Localized changes in peroxidase activity accompany hydrogen peroxide generation during the development of a nonhost hypersensitive reaction in lettuce
Bestwick (1998)
A proteomic analysis of the wound response in Medicago leaves reveals the early activation of a ROS-sensitive signal pathway.
N. Soares (2011)
Classical inhibitors of NOX NAD(P)H oxidases are not specific.
E. Aldieri (2008)
Involvement of hydrogen peroxide, calcium, and ethylene in the induction of the alternative pathway in chilling-stressed Arabidopsis callus
Huahua Wang (2011)
Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress.
L. Carassay (2012)
Root growth restraint can be an acclimatory response to low pH and is associated with reduced cell mortality: a possible role of class III peroxidases and NADPH oxidases.
J. P. Graças (2016)
Possible functions of extracellular peroxidases in stress-induced generation and detoxification of active oxygen species
A. Mika (2004)
Spatiotemporal patterning of reactive oxygen production and Ca(2+) wave propagation in fucus rhizoid cells.
Susana M. Coelho (2002)
Plant Tissue Cultures as Models for Tree Physiology: Somatic Embryogenesis of Tilia cordata and Lignin Biosynthesis in Picea abies Suspension Cultures as Case Studies
A. Kärkönen (2002)
The Oxidative Processes Induced in Cell Suspensions of Solanum Species by Culture Filtrate of Phytophthora infestans
L. Polkowska-Kowalczyk (2001)
Potential role of NADPH-oxidase in early steps of lead-induced oxidative burst in Vicia faba roots.
B. Pourrut (2008)
Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth.
Martin Potocký (2007)
Specific localization and measurement of hydrogen peroxide in Arabidopsis thaliana cell suspensions and protoplasts elicited by COS-OGA
Quentin Ledoux (2014)
Reactive Oxygen Species in the Elongation Zone of Maize Leaves Are Necessary for Leaf Extension 1
A. E. Rodríguez (2002)
Origin of cadmium-induced reactive oxygen species production: mitochondrial electron transfer versus plasma membrane NADPH oxidase.
Eiri Heyno (2008)
See more
Semantic Scholar Logo Some data provided by SemanticScholar