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Purification And Biochemical Properties Of Microbial Pectinases—a Review

Sathyanarayana N. Gummadi, T. Panda
Published 2003 · Chemistry

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Abstract Pectinases are a complex group of enzymes that degrade various pectic substances present in plant tissues. Pectinases have potential applications in fruit, paper and textile industries. Apart from these industrial applications, these enzymes possess biological importance in protoplast fusion technology and plant pathology. Since applications of pectinases in various fields are widening, it is important to understand the nature and properties of these enzymes for efficient and effective usage. For the past few years, vigorous research has been carried out on isolation and characterization of pectinases. New affinity matrices with improved characteristics and affinity-precipitation techniques have been developed for purification of pectinases. Recently much attention has been focused on chemical modification of pectinases and their catalytic performance by various researchers. These studies are helpful in determining key amino acid residues responsible for substrate binding, catalytic action, and physico-chemical environmental conditions for maximum hydrolysis. This short review highlights progress on purification and understanding the biochemical aspects of microbial pectinases.
This paper references
10.1016/S0141-0229(99)00131-3
Pectinase in papermaking: solving retention problems in mechanical pulps bleached with hydrogen peroxide.
Reid (2000)
10.4315/0362-028X-46.5.453
Determining the Safety of Enzymes Used in Food Processing.
M. Pariza (1983)
10.1271/BBB.64.1729
Purification and Characterization of an Endo-Polygalacturonase from Aspergillus awamori
M. Nagai (2000)
10.1016/S0168-6445(97)00005-3
Expression cloning of fungal enzyme genes; a novel approach for efficient isolation of enzyme genes of industrial relevance
H. Dalbøge (1997)
10.1271/bbb.64.2360
Purification and Characterization of Thermostable Pectate Lyase with Protopectinase Activity from Thermophilic Bacillus sp. TS 47
M. Takao (2000)
10.1074/jbc.274.52.37053
Performance of Selected Microbial Pectinases on Synthetic Monomethyl-esterified Di- and Trigalacturonates*
H. Kester (1999)
10.1007/978-94-011-6740-6_8
Application of Enzymes in Fruit Juice Technology
Joachim W. Baumann (1981)
10.1016/S0960-8524(00)00151-6
Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast.
S. Hari Krishna (2001)
10.3109/07388558909148194
Enzymatic degradation of cell wall and related plant polysaccharides.
O. Ward (1989)
10.1016/S0065-2164(08)70597-5
Pectin, pectinase and protopectinase: production, properties, and applications.
T. Sakai (1993)
10.1007/BF02310007
Production, purification and partial characterization of an endopolygalacturonase fromCryptococcus albidus var.albidus
F. Federici (1985)
10.1023/A:1018587425202
Differences between pectic enzymes produced by laboratory and wild-type strains of Saccharomyces cerevisiae
P. Blanco (1997)
PURIFICATION AND CERTAIN PROPERTIES OF PECTIN TRANS-ELIMINASE FROM ASPERGILLUS FONSECAEUS.
R. Edstrom (1964)
10.1016/0141-0229(88)90060-9
Pectic enzymes from Aureobasidium pullulans LV 10
P. L. Manachini (1988)
10.1042/bj0990562
The pectic enzymes of Aspergillus niger. A second exopolygalacturonase.
P. J. Mill (1966)
10.1042/bj2790343
Pectin methylesterase, metal ions and plant cell-wall extension. Hydrolysis of pectin by plant cell-wall pectin methylesterase.
J. Nari (1991)
10.1016/0014-5793(96)00171-8
Inversion of configuration during hydrolysis of α‐1,4‐galacturonidic linkage by three Aspergillus polygalacturonases
P. Biely (1996)
10.1016/0014-5793(96)00146-9
An essential tyrosine residue of Aspergillus polygalacturonase
E. Stratilová (1996)
10.1271/bbb1961.55.25
Purification and Characterization of Endo-Pectate Lyase from Bacillus macerans
Y. Miyazaki (1991)
10.1271/BBB.65.842
Purification and Properties of a Galacturonic Acid-releasing Exopolygalacturonase from a Strain of Bacillus
T. Kobayashi (2001)
10.1007/PL00009023
Production of pectolytic enzymes – a review
G. Naidu (1998)
10.1007/BF00163286
Factors affecting pectate lyase activity during membrane filtration
S. Denis (1990)
10.1042/bj0990557
The pectic enzymes of Aspergillus niger. A mercury-activated exopolygalacturonase.
P. J. Mill (1966)
10.1271/bbb.58.353
Purification, characterization, and production of two pectic transeliminases with protopectinase activity from Bacillus subtilis.
T. Sakamoto (1994)
10.1111/J.1574-6976.1997.TB00343.X
Expression cloning of fungal enzyme genes; a novel approach for efficient isolation of enzyme genes of industrial relevance.
H. Dalbøge (1997)
10.1007/BF01575945
Catabolite repression of the synthesis of inducible polygalacturonase and pectinesterase byAspergillus niger sp.
M. C. Maldonado (2005)
10.1067/MAI.2001.114114
Group 13 grass allergens: structural variability between different grass species and analysis of proteolytic stability.
A. Petersen (2001)
10.1093/oxfordjournals.pcp.a079318
Isolation of tobacco mesophyll cells in intact and active state
I. Takebe (1968)
10.1007/BF02941808
Production of ethanol from enzymatically hydrolyzed orange peel by the yeastSaccharomyces cerevisiae
K. Grohmann (1994)
10.1073/pnas.97.7.3526
AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora.
Y. Dong (2000)
10.1016/0141-0229(80)90108-8
Enzymes and Food Processing
G. Birch (1980)
10.1111/j.1574-6968.1985.tb00680.x
Purification and characterization of pectinesterase and polygalacturonase from Trichoderma reesei
O. Markovič (1985)
10.1099/00221287-137-8-1815
Pectinase production by Neurospora crassa: purification and biochemical characterization of extracellular polygalacturonase activity.
M. Polizeli (1991)
10.1271/bbb1961.48.2113
Isolation and Fundamental Properties of enofo-Pectate Lyase pl-Isozymes from Erwinia carotovora
Hiroyuki Tanabe (1984)
10.1111/j.1365-2621.1978.tb02402.x
PURIFICATION AND CHARACTERIZATION OF ENDO-POLYGALACTURONASE FROM Rhizopus arrhizus
Yuan K. Liu (1978)
10.1016/0167-4838(96)00067-2
Implication of tryptophan and histidine in the active site of endo-polygalacturonase from Aspergillus ustus: elucidation of the reaction mechanism.
M. Rao (1996)
10.1135/cccc19674504
Characterization of extracellular polygalacturonases of Aspergillus niger
Ľ. Rexová-Benková (1967)
10.1016/0008-6215(94)00006-2
Characterisation of the endo-polygalacturonase-resistant region of the pectin from Bupleurum falcatum L.--a polysaccharide with an active function in clearance of immune complexes.
T. Matsumoto (1995)
10.1016/S0960-8524(00)00118-8
Applications of pectinases in the commercial sector: a review.
D. Kashyap (2001)
10.1006/JMBI.1998.1791
Macromolecular assembly and secretion across the bacterial cell envelope: type II protein secretion systems.
M. Russel (1998)
10.1002/YEA.693
Regulation of the expression of endopolygalacturonase gene PGU1 in Saccharomyces.
S. Gognies (2001)
10.1094/Phyto-80-1374
Polygalacturonases associated with infection of Valencia orange by Penicillium italicum.
J. Hershenhorn (1990)
10.1016/0141-0229(94)90093-0
Concomitant biosynthesis of Aspergillus niger pectolytic enzymes and citric acid on sucrose
J. Friedrich (1994)
10.1007/BF00270782
Submerged production of pectolytic enzymes by Aspergillus niger: effect of different aeration/agitation regimes
J. Friedrich (2004)
10.1128/aem.58.2.578-583.1992
Purification and Characterization of Extracellular Pectinolytic Enzymes Produced by Sclerotinia sclerotiorum.
C. Riou (1992)
10.1007/s002530051628
Perspectives in the biological function and the technological application of polygalacturonases
C. Lang (2000)
10.1111/j.1365-2958.1994.tb01033.x
beta-Lactamase topology probe analysis of the OutO NMePhe peptidase, and six other Out protein components of the Erwinia carotovora general secretion pathway apparatus.
P. J. Reeves (1994)
10.1016/S0032-9592(99)00050-3
Multiresponse analysis of microbiological parameters affecting the production of pectolytic enzymes by Aspergillus niger: a statistical view
T. Panda (1999)
10.1002/(SICI)1099-1352(199634/12)9:5/6<356::AID-JMR328>3.0.CO;2-X
Affinity precipitation of proteins
M. Gupta (1996)
10.1073/PNAS.97.16.8762
Structure and function of pectic enzymes: virulence factors of plant pathogens.
S. Herron (2000)
10.1128/aem.61.9.3316-3322.1995
Isolation and characterization of polygalacturonase genes (pecA and pecB) from Aspergillus flavus.
M. Whitehead (1995)
10.1016/0006-291X(85)91289-6
New matrices for the purification of pectinases by affinity chromatography.
J. Lobarzewski (1985)
ELIMINATIVE CLEAVAGE OF PECTIN AND OF OLIGOGALACTURONIDE METHYL ESTERS BY PECTIN TRANS-ELIMINASE.
R. Edstrom (1964)
10.1046/J.1432-1327.1999.00080.X
Kinetic characterization of Aspergillus niger N400 endopolygalacturonases I, II and C.
J. Benen (1999)
10.1007/S004490050693
Behaviour of pectinases under process conditions
G. Naidu (1999)
10.1002/(SICI)1097-4660(199911)74:11<1055::AID-JCTB148>3.0.CO;2-Z
Simultaneous saccharification and fermentation of pretreated Antigonum leptopus (Linn) leaves to ethanol
S. H. Krishna (1999)
10.1016/0032-9592(94)80002-2
Ultrafiltration preparation of pectinolytic enzymes from citric acid fermentation broth
J. Bohdziewicz (1994)
10.1271/BBB.59.1242
Substrate binding ability of chemically inactivated pectinase for the substrate pectic acid.
Y. Chiba (1995)
10.1081/PB-100104907
PERFUSION CHROMATOGRAPHY SEPARATION OF THE TOMATO FRUIT-SPECIFIC PECTIN METHYLESTERASE FROM A SEMIPURIFIED COMMERCIAL ENZYME PREPARATION
B. Savary (2001)
10.1002/(SICI)1097-0061(19990115)15:1<11::AID-YEA336>3.0.CO;2-O
Cloning, sequence analysis and overexpression of a Saccharomyces cerevisiae endopolygalacturonase-encoding gene (PGL1).
S. Gognies (1999)
10.1023/A:1008187929132
High-speed pectic enzyme fractionation by immobilised metal ion affinity membranes
S. Camperi (2000)
10.1107/S0907444900013305
Crystallization and preliminary X-ray analysis of endopolygalacturonase SE1 from Trichosporon penicillatum.
C. T. Lu (2000)
10.1007/S004499900119
Rotating simplex method of optimization of physical parameters for higher production of extracellular pectinases in bioreactor
T. Panda (2000)
10.1016/0141-0229(87)90129-3
Stimulation of the production of extracellular pectinolytic activities of Aspergillus sp. by galacturonic acid and glucose addition
G. Aguilar (1987)
10.1094/MPMI.2000.13.8.887
Expression of pectate lyase from Colletotrichum gloesosporioides in C. magna promotes pathogenicity.
N. Yakoby (2000)
10.1007/BF02941826
The effect of cell density on the production of xylitol fromd-xylose by yeast
N. Cao (1994)
10.3929/ethz-a-000087857
Fraktionierung und Charakterisierung der pektolytischen Enzyme von Aspergillus niger
Albert Koller (1966)
10.1007/BF02921505
Facile isolation ofEndo-pectate lyase fromErwinia carotovora based on electrostatic interaction
S. Fukuoka (1990)
10.1002/jsfa.2740630404
Characterisation and enzymatic hydrolysis of nettle (Urtica dioica L) deproteinated biomass.
P. D. Leo (1993)
10.1111/j.1745-4530.1980.tb00228.x
KINETICS OF THERMAL INACTIVATION FOR A PECTIC ENZYME IN SWEET CHERRY BRINES1
P. E. Athanosopoulos (1980)
10.3109/10242429509003191
Purification and Immobilization of Aspergillus Niger Pectinase on Magnetic Latex Beads
R. Tyagi (1995)
10.1135/cccc19660122
Isolation of extracellular pectolytic enzymes produced by Aspergillus niger
Ľ. Rexová-Benková (1966)
10.1016/S0141-0229(99)00017-4
Performance of pectolytic enzymes during hydrolysis of pectic substances under assay conditions: a statistical approach
G.Sathya Narayana Naidu (1999)
10.1007/BF00368763
Effect of carbon sources on the synthesis of pectinase by Aspergilli
S. R. Nair (1995)
10.1007/BF01024570
Purification and properties of an endopolygalacturonase produced byRhizopusstolonifer
P. L. Manachini (2005)
10.1016/S1369-703X(98)00019-9
Application of response surface methodology to evaluate some aspects on stability of pectolytic enzymes from Aspergillus niger
G. Sathya (1998)
10.1271/bbb.60.794
Mold Pectinase Modified with Dialdehyde Derivatives of Dextran and Cellulose.
M. Kobayashi (1996)
10.1128/AEM.61.10.3580-3585.1995
Purification and characterization of an extracellular pectate lyase from an Amycolata sp.
F. Brühlmann (1995)
10.1023/A:1008171105319
Recovery of endo-polygalacturonase using polyethylene glycol-salt aqueous two-phase extraction with polymer recycling
Y. Wu (2000)
10.1016/0141-0229(90)90065-X
Kinetic and operational study of a cross-flow reactor with immobilized pectolytic enzymes.
P. Lozano (1990)
10.1080/02648725.1999.10647984
Pectins, pectinases and plant-microbe interactions.
R. Prade (1999)
10.1016/S1369-703X(00)00129-7
Effects of N-acetylation degree on N-acetylated chitosan hydrolysis with commercially available and modified pectinases.
Shin-ya (2001)
10.1007/S004490050305
Statistical optimization of medium components for improved synthesis of pectinase by Aspergillus niger
S. Nair (1997)
10.1128/JB.178.6.1593-1599.1996
The Erwinia chrysanthemi pecT gene regulates pectinase gene expression.
N. Surgey (1996)



This paper is referenced by
10.1007/s00253-006-0643-8
Enhanced ethanol fermentation of brewery wastewater using the genetically modified strain E. coli KO11
K. Rao (2007)
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K. Mehraj Pasha (2013)
10.5151/CHEMENG-COBEQ2014-0719-24302-141467
APLICAÇÃO DE PREPARAÇÃO PECTINOLÍTICA FÚNGICA OBTIDA POR CULTIVO EM ESTADO SÓLIDO NA EXTRAÇÃO DE SUCO DE UVA
Patrícia Rios Poletto (2014)
Isolation, production and partial purification of fungal extracellular pectinolytic enzymes from the forest soils of Bhadra Wildlife Sanctuary, Western Ghats of Southern India
S. Banakar (2014)
Influence of Mode of Fermentation on Production of Polygalacturonase by a Novel Strain of Streptomyces lydicus
Nicemol Jacob (2006)
Bacterial Pectinases and their Potent Biotechnological Application in Fruit Processing/Juice Production Industry: A Review
Katragadda Suneetha (2011)
10.5897/AJB12.355
Characterization of polygalacturonases from fruit spoilage Fusarium oxysporum and Aspergillus tubingensis
Ahmed Rashed Al-Najada (2012)
Screening of Aspergillus niger Strains for Pectinolytic Activity by Solid State Fermentation
T. Pramod (2014)
Estudos de imobilização de pectinase de Aspergillus terreus
Raissa Pieroni Vaz (2019)
10.30848/PJB2019-2(41)
Optimization of pectinase production from Geotrichum candidum AA15 using response surface methodology
A. Ahmed (2019)
SCREENING OF THERMOPHILLIC BACTERIAL STRAINS ISOLATED FROM THE DESERT OF NORTHERN INDIA FOR INDUSTRIALLY APPLICABLE
Nevadita Sharma (2016)
10.14419/IJBR.V4I1.6134
Production of ethanol and clarification of apple juice by pectinase enzyme produced from Aspergillus terreus NCFT 4269.10
B. K. Sethi (2016)
Pectinase Activity of some Micromycetes Isolated from Agricultural Soils
N. Nayebyazdi (2012)
10.1155/2014/481508
Evaluating the Biodeterioration Enzymatic Activities of Fungal Contamination Isolated from Some Ancient Yemeni Mummies Preserved in the National Museum
Khalid Mohammed Naji (2014)
VALORIZATION OF CARROT PROCESSING WASTE A Thesis presented to the Faculty of California Polytechnic State University, San Luis Obispo In Partial Fulfillment of the Requirements for the Degree Master of Science in Agriculture with a Specialization in Food Science by
Alexandra Duval (2020)
10.3923/BIOTECH.2007.245.250
Effect of Aeration on the Production of Endo-Pectinase from Coffee Pulp by a Novel Thermophilic Fungi Mycotypha sp. Strain No. AKM 1801
.. C.Venugopal (2007)
10.1016/J.PROCBIO.2008.01.010
Production and chromatographic behaviour of polygalacturonase from Pleurotus ostreatus on immobilized metal chelates
Maria do Rosário Freixo (2008)
10.3724/SP.J.1009.2009.00381
Biotransformation of Dichotomin by Pectinex BE XXL
Yang Zhao (2010)
10.18860/SAINS.V0I0.2303
HIDROLISIS GUGUS METOKSIL PEKTIN AMPAS TEBU UNTUK MENGHASILKAN BIOMETANOL
Akyunul Jannah (2013)
10.5151/chemeng-cobeq2014-0699-24383-142427
OBTENÇÃO DE PECTINASES EM CULTIVO SUBMERSO DE Aspergillus oryzae SOB DIFERENTES CONDIÇÕES DE pH
Lenara Meneghel (2014)
The transcription factor, fixK, plays a central role in carbon source metabolism and cellulose synthesis in Komagataeibacter species
Andrew J Varley (2017)
10.1093/jb/mvaa097
Kinetics and thermodynamics of thermal inactivation for recombinant Escherichia coli cellulases, cel12B, cel8C, and polygalacturonase, peh28; biocatalysts for biofuel precursor production.
E. Ibrahim (2020)
STUDIES ON PECTINOLYTIC AND PROTEOLYTIC ENZYMES FROM DETERIORATED GRAPES (Vitis vinifera)
A. Ajayi (2013)
10.22207/JPAM.11.3.02
Use of Highly Methoxyl-esterified Cross-linked Alcohol Insoluble Substance to Remove Pectinesterase for the Reduction of Methanol in Sweet Orange Wine
C. F. Wu (2017)
10.1016/B978-0-12-803725-6.00011-X
Microbial Enzymes of Use in Industry
X. Liu (2017)
10.1134/S0003683813040145
Purification and characterization of pectin lyase secreted by Aspergillus flavus MTCC 10938
Sharda Yadav (2013)
10.1016/J.BCAB.2014.09.003
Immobilization of pectinase from Bacillus licheniformis KIBGE-IB21 on chitosan beads for continuous degradation of pectin polymers
Haneef ur Rehman (2014)
DISCOVERY OF POLYGALACTURONASE PRODUCING BACILLUS TEQUILENSIS STRAIN ARMATI USING 16S rRNA GENE SEQUENCING
Ameer Khusro (2015)
10.1016/J.MOLCATB.2016.02.012
Catalytic properties of a highly thermoactive polygalacturonase from the mesophilic fungus Penicillium occitanis and use in juice clarification
Hajer Tounsi (2016)
10.22161/IJEAB/2.3.1
Pectinase: Substrate, Production and their Biotechnological Applications
Oliyad Jeilu Oumer (2017)
10.1590/S1516-89132011000100019
Biotechnological potential of alternative carbon sources for production of pectinases by Rhizopus microsporus var. rhizopodiformis
André Ricardo de Lima Damásio (2011)
PRODUCTION AND CHARACTERIZATION OF EXOPOLYGALACTURONASE FROM FUSARIUM OXYSPORUM F. SP. LYCOPERSICI.
Dhamodaran Nirmaladevi (2014)
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