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Fluorescent Protein Applications In Plants.
R. Berg, R. Beachy
Published 2008 · Biology, Medicine
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Study of plant cell biology has benefited tremendously from the use of fluorescent proteins (FPs). Development of well-established techniques in genetics, by transient expression or by Agrobacterium-mediated plant cell transformation, makes it possible to readily create material for imaging molecules tagged with FPs. Confocal microscopy of FPs is routine and, in highly scattering tissues, multiphoton microscopy improves deep imaging. The abundance of autofluorescent compounds in plants in some cases potentially interferes with FP signals, but spectral imaging is an effective tool in unmixing overlapping signals. This approach allows separate detection of DsRed and chlorophyll, DsRed and GFP, and green fluorescent protein (GFP) and yellow fluorescent protein (YFP). FPs have been targeted to most plant organelles. Free (untargeted) FPs in plant cells are not only cytoplasmic, but also go into the nucleus due to their small size. FP fluorescence is potentially unstable in acidic vacuoles. FPs have been targeted to novel compartments, including protein storage vacuoles in seeds. Endoplasmic reticulum (ER)-targeted GFP has identified novel inclusion bodies that are surprisingly dynamic. FP-tagged Rab GTPases have allowed documentation of the dynamics of membrane trafficking. Investigation of virus infections has progressed significantly with the aid of FP-tagged virus proteins. Advanced techniques are giving plant scientists the ability to quantitatively analyze the behavior of FP-tagged proteins. Fluorescence lifetime microscopy is becoming the method of choice for fluorescence resonance energy transfer (FRET) analysis of FP-tagged proteins. Fluorescence correlation spectroscopy (FCS) of FPs provides information on molecular diffusion and intermolecular interactions. Use of FPs in elucidating the behavior of plant cells has a bright future.
This paper references
Shining light on signaling and metabolic networks by genetically encoded biosensors.
S. Lalonde (2005)
Molecular genetic analysis of left-right handedness in plants.
T. Hashimoto (2002)
Confocal fluorescence microscopy of plant cells
P. Hepler (2005)
In vivo fluorescence correlation microscopy (FCM) reveals accumulation and immobilization of Nod factors in root hair cell walls.
J. Goedhart (2000)
Imaging plant cells
N. Moreno (2006)
High-Throughput Fluorescent Tagging of Full-Length Arabidopsis Gene Products in Planta1
Guo-Wei Tian (2004)
Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly.
J. Haseloff (1997)
Tools to Study Plant Organelle Biogenesis. Point Mutation Lines with Disrupted Vacuoles and High-Speed Confocal Screening of Green Fluorescent Protein-Tagged Organelles1[w]
Emily L Avila (2003)
Gene transfer and expression in plants.
A. Lorence (2004)
Multi-Photon Molecular Excitation in Laser-Scanning Microscopy
W. Denk (2006)
High-Throughput Viral Expression of cDNA–Green Fluorescent Protein Fusions Reveals Novel Subcellular Addresses and Identifies Unique Proteins That Interact with Plasmodesmata Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.013284.
N. Escobar (2003)
Fluorescence correlation spectroscopy with autofluorescent proteins.
T. Kohl (2005)
The uses of green fluorescent protein in plants.
J. Haseloff (2006)
Analysis of MADS box protein–protein interactions in living plant cells
R. Immink (2002)
Imaging protein-protein interactions in living cells
M. Hink (2004)
Excited states and free radicals in biology and medicine: edited by R.V. Bensasson, E.J. Land and T.G. Truscott, Oxford University Press, 1993
R. Brown (1995)
Fluorescence lifetime-resolved imaging: measuring lifetimes in an image.
R. Clegg (2003)
Fluorescence lifetime imaging microscopy (FLIM).
E. V. van Munster (2005)
Molecular biology and mutation of green fluorescent protein.
D. A. Zacharias (2006)
Annotation of the Arabidopsis Genome1
J. Wortman (2003)
Quantitative fluorescence microscopy: from art to science.
M. Fricker (2006)
Ecdysone agonist-inducible expression of a coat protein gene from tobacco mosaic virus confers viral resistance in transgenic Arabidopsis.
J. C. Koo (2004)
GFP technology for live cell imaging.
D. Ehrhardt (2003)
Green-fluorescent protein fusions for efficient characterization of nuclear targeting.
R. J. Grebenok (1997)
Comparison of Widefield/Deconvolution and Confocal Microscopy for Three-Dimensional Imaging
Peter Shaw (2006)
The identity of mystery organelles in Arabidopsis plants expressing GFP
B. E. Gunning (1998)
Green fluorescent protein : properties, applications, and protocols
M. Chalfie (2005)
Recruitment and interaction dynamics of plant penetration resistance components in a plasma membrane microdomain.
Riyaz A. Bhat (2005)
Viral Movement Proteins as Probes for Intracellular and Intercellular Trafficking in Plants
S. Lazarowitz (1999)
Coat protein regulates formation of replication complexes during tobacco mosaic virus infection.
S. Asurmendi (2004)
Two Fluorescent Markers Identify the Vacuolar System of Schizophyllum commune
Amy L. Inselman (1999)
Evaluation of spectral imaging for plant cell analysis
R. Berg (2004)
Cell damage and reactive oxygen species production induced by fluorescence microscopy: effect on mitosis and guidelines for non-invasive fluorescence microscopy.
R. Dixit (2003)
New tools for in vivo fluorescence tagging.
S. Chapman (2005)
Using intrinsically fluorescent proteins for plant cell imaging.
R. Dixit (2006)
NAI1 Gene Encodes a Basic-Helix-Loop-Helix–Type Putative Transcription Factor That Regulates the Formation of an Endoplasmic Reticulum–Derived Structure, the ER Body
R. Matsushima (2004)
pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants
T. Tzfira (2005)
11 – Real-Time Fluorescence Lifetime Imaging and FRET Using Fast-Gated Image Intensifiers
Glen I. Redford (2005)
Tobacco mosaic virus infection induces severe morphological changes of the endoplasmic reticulum.
C. Reichel (1998)
A monomeric red fluorescent protein
R. E. Campbell (2002)
More Than You Ever Really Wanted to Know About Charge-Coupled Devices
J. Pawley (2006)
Engineered GFP as a vital reporter in plants
W. Chiu (1996)
Discovery and properties of GFP-like proteins from nonbioluminescent anthozoa.
K. Lukyanov (2006)
Two-photon excitation action cross-sections of the autofluorescent proteins
G. Blab (2001)
Methods in Enzymology , Vol
S. Colowick (1966)
The Arabidopsis Rab GTPase RabA4b Localizes to the Tips of Growing Root Hair Cells
M. Preuss (2004)
Green Fluorescent Protein Targeted to the Nucleus, a Transgenic Phenotype Useful for Studies in Plant Biology
E. Chytilová (1999)
Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants.
K. Tamura (2003)
The role of ARPC4 in tip growth and alignment of the polar axis in filaments of Physcomitrella patens.
Pierre-François Perroud (2006)
Imaging plant cells by two-photon excitation
J. Feijó (2003)
Molecular imaging : FRET microscopy and spectroscopy
A. Periasamy (2006)
Chemical-Inducible, Ecdysone Receptor-Based Gene Expression System for Plants
M. Padidam (2004)
Active protein transport through plastid tubules: velocity quantified by fluorescence correlation spectroscopy.
Rainer H. Köhler (2000)
Distribution of TMV movement protein in single living protoplasts immobilized in agarose.
P. Más (1998)
Green fluorescent protein as a marker for gene expression.
M. Chalfie (1994)
An improved cyan fluorescent protein variant useful for FRET
M. Rizzo (2004)
Transactivated and chemically inducible gene expression in plants.
I. Moore (2006)
Special issue: celebrating 50 years of the IUBMBRNA silencing
D. Baulcombe (2005)
Plant Microtechnique and Microscopy
S. Ruzin (1999)
Gateway-compatible vectors for plant functional genomics and proteomics.
K. Earley (2006)
Spectral imaging and its applications in live cell microscopy
T. Zimmermann (2003)
Confocal and two-photon microscopy : foundations, applications, and advances
A. Diaspro (2001)
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
Nathan Christopher Shaner (2004)
This paper is referenced by
Exploring the Spatiotemporal Organization of Membrane Proteins in Living Plant Cells.
Li Wang (2018)
An efficient method for visualization and growth of fluorescent Xanthomonas oryzae pv. oryzae in planta
Sang-Wook Han (2008)
Exploring plant endomembrane dynamics using the photoconvertible protein Kaede.
Spencer Brown (2010)
Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii.
B. Rasala (2013)
Production de protéines recombinantes par des plantes carnivores génétiquement transformées : application à Drosera rotundifolia et transfert de la technologie à Nepenthes alata
Flore Biteau (2009)
Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis
Paul A Carroll (2018)
Elucidation of Calcium-Signaling Components and Networks
I. Day (2011)
Plant cell types: reporting and sampling with new technologies.
T. Nelson (2008)
Characterization of complex systems using the design of experiments approach: transient protein expression in tobacco as a case study.
J. Buyel (2014)
A bacterial signal peptide is functional in plants and directs proteins to the secretory pathway
L. Moeller (2009)
The Identification and Characterization of New Small Molecule Probes of Cell Expansion and Cytokinesis
Simon Alfred (2011)
Autofluorescence in Plants
L. Donaldson (2020)
Emission spectra profiling of fluorescent proteins in living plant cells
Evelien Mylle (2013)
Developing Chlamydomonas reinhardtii for recombinant therapeutic protein production
Daniel J. Barrera (2014)
The interplay between a Phytophthora RXLR effector and an Arabidopsis lectin receptor kinase.
K. Bouwmeester (2010)
Perfluorodecalin enhances in vivo confocal microscopy resolution of Arabidopsis thaliana mesophyll.
George R Littlejohn (2010)
GEN Ds-Red Ở PROTOCORM-LIKE BODY CHUYỂN GEN CỦA PHONG LAN Dendrobium CV . BURANA WHITE
Nguyễn Hữu Hổ (2012)
Strasburger's Plant Sciences
A. Bresinsky (2013)
Elisabeth Truernit (2014)
Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants
Sandra K. Tanz (2013)
Two-photon imaging with longer wavelength excitation in intact Arabidopsis tissues
Yoko Mizuta (2014)
Molecular Imaging: Chemistry and Applications
W. He (2013)
INTRACELLULAR LOCALIZATION OF AN ENDOGENOUS CELLULOSE SYNTHASE OF MICRASTERIAS DENTICULATA (DESMIDIALES, CHLOROPHYTA) BY MEANS OF TRANSIENT GENETIC TRANSFORMATION 1
K. Vannerum (2010)
A dual-targeted soybean protein is involved in Bradyrhizobium japonicum infection of soybean root hair and cortical cells.
M. Libault (2011)
Evolving trends in biosciences: multi-purpose proteins - GFP and GFP-like proteins
K. Krishna (2009)
Solubility, particle formation and immune display of trimers of major capsid protein 7 of African horsesickness virus fused with enhanced green fluorescent protein
E. Mizrachi (2011)
Advances in Imaging Plant Cell Walls.
Y. Zhao (2019)
An unbiased method for the quantitation of disease phenotypes using a custom-built macro plugin for the program ImageJ.
A. Abd-El-Haliem (2012)
Seeing is believing: on the use of image databases for visually exploring plant organelle dynamics.
S. Mano (2009)
BODIPY probes to study peroxisome dynamics in vivo.
M. Landrum (2010)
Simple and reliable system for transient gene expression for the characteristic signal sequences and the estimation of the localization of target protein in plant cell
A. Tyurin (2017)
Computational modelling of the BRI1 receptor system.
G. W. van Esse (2013)See more