Online citations, reference lists, and bibliographies.

Cytocompatibility And Response Of Osteoblastic-like Cells To Starch-based Polymers: Effect Of Several Additives And Processing Conditions.

Manuela E Gomes, Rui Luís Reis, António M. Cunha, Clemens A. van Blitterswijk, Joost D de Bruijn
Published 2001 · Materials Science, Medicine
Cite This
Download PDF
Analyze on Scholarcy
This work reports on the biocompatibility evaluation of new biodegradable starch-based polymers that are under consideration for use in orthopaedic temporary applications and as tissue engineering scaffolds. It has been shown in previous works that by using these polymers it is both possible to produce polymer/hydroxyapatite (HA) composites (with or without the use of coupling agents) with mechanical properties matching those of the human bone, and to obtain 3D structures generated by solid blowing agents, that are suitable for tissue engineering applications. This study was focused on establishing the influence of several additives (ceramic fillers, blowing agents and coupling agents) and processing methods/conditions on the biocompatibility of the materials described above. The cytotoxicity of the materials was evaluated using cell culture methods, according to ISO/EN 109935 guidelines. A cell suspension of human osteosarcoma cells (HOS) was also seeded on a blend of corn starch with ethylene vinyl alcohol (SEVA-C) and on SEVA-C/HA composites, in order to have a preliminary indication on cell adhesion and proliferation on the materials surface. In general, the obtained results show that all the different materials based on SEVA-C, (which are being investigated for use in several biomedical applications), as well as all the additives (including the novel coupling agents) and different processing methods required to obtain the different properties/products, can be used without inducing a cytotoxic behaviour to the developed biomaterials.
This paper references
A new technique for direct measurement of the shear force necessary to detach a cell from a material.
Akira Yamamoto (1998)
Biomaterials science: overview and opportunities with special reference to organic and polymeric glow discharge plasma treatments
BD Ratner (1997)
Cell culture methods for testing biocompatibility.
Arturo Pizzoferrato (1994)
Using Nonconventional Processing to Develop Anisotropic and Biodegradable Composites of Starch-Based Thermoplastics Reinforced with Bone-Like Ceramics
Rui Luís Reis (1998)
Current trends in biocompatibility test
CJ Kirkpatrick (1998)
Quantitative methods for in vitro cytotoxicity testing of biomaterials
A Dekker (1994)
Effects of biodegradable polymer particles on rat marrow-derived stromal osteoblasts in vitro.
M. Conley Wake (1998)
Current trends in biocompatibility testing
C James Kirkpatrick (1998)
Porous chitosan sca!olds for tissue engineering
Sundararajan V. Madihally (1999)
Biocompatibility testing of novel starch based polymers and composites with potential application in orthopaedic surgery
S Mendes (2001)
From cytotoxicity to biocompatibility testing in vitro: cell adhesion molecule expression defines a new set of parameters
Theo G. van Kooten (1997)
Degradation model of starch-EVOH+HA composites
Claudia Maria Vaz (2001)
Behaviour of human osteoblasts cultured on bioactive glass coatings.
Adriana Oliva (1998)
A critical view of current and proposed methodologies for biocompatibility testing: cytotoxicity in vitro
CJ Kirkpatrick (1992)
Theoretical and practical aspects of testing potential biomaterialsin vitro
C James Kirkpatrick (1990)
A new approach based on injection moulding to produce starch based biodegradable sca!olds: morphology, mechanical and degradation behaviour
ME Gomes (2000)
In vitro models of biocompatibility: a review.
Carl Thomas Hanks (1996)
New starch-based thermoplastic hydrogels for use as bone cements or drug-delivery carriers
C. Pereira (1998)

This paper is referenced by
The effect of starch and starch-bioactive glass composite microparticles on the adhesion and expression of the osteoblastic phenotype of a bone cell line.
Gabriela A. Silva (2007)
Biofunctionality and immunocompatibility of starch-based biomaterials
Alfredo Pinheiro Marques (2004)
Hierarchical starch-based fibrous scaffold for bone tissue engineering applications.
Albino Martins (2009)
A cartilage ECM-derived 3-D porous acellular matrix scaffold for in vivo cartilage tissue engineering with PKH26-labeled chondrogenic bone marrow-derived mesenchymal stem cells.
Qiang Yang (2008)
Cell viability and hemocompatibility evaluation of a starch-based hydrogel loaded with hydroxyapatite or calcium carbonate for maxillofacial bone regeneration
Juan Carlos Flores-Arriaga (2017)
Polysaccharides and their derivatives for versatile tissue engineering application.
Ferdous Hossain Khan (2013)
Entrapment ability and release profile of corticosteroids from starch-based microparticles.
Gabriel A. Silva (2005)
Evaluation of extracellular matrix formation in polycaprolactone and starch-compounded polycaprolactone nanofiber meshes when seeded with bovine articular chondrocytes.
Marta Maria Alves da Silva (2009)
A novel porous cells scaffold made of polylactide-dextran blend by combining phase-separation and particle-leaching techniques.
Qing Hua Cai (2002)
Chitosan-poly(butylene succinate) scaffolds and human bone marrow stromal cells induce bone repair in a mouse calvaria model.
Ana Rita Costa-Pinto (2012)
Nano-engineered hybrid hydroxyapatite-grafted biocomposites for Euspria pulchella mimicking through chaotic flow regimes
Yutika Badhe (2015)
Green processing of porous chitin structures for biomedical applications combining ionic liquids and supercritical fluid technology.
Simone S Silva (2011)
Nature-inspired calcium phosphate coatings : present status and novel advances in the science of mimicry
Ana Oliveira (2003)
Enzymatic Degradation of Starch Based Thermoplastic Compounds Used in Protheses
M. Alberta Araújo (2004)
Effect of flow perfusion on the osteogenic differentiation of bone marrow stromal cells cultured on starch-based three-dimensional scaffolds.
Manuela E Gomes (2003)
Preparation and characterization of starch-polye-caprolactone microparticles incorporating bioactive agents for drug delivery and tissue engineering applications
Elizabeth R Balmayor (2011)
Bioinert, biodegradable and injectable polymeric matrix composites for hard tissue replacement: state of the art and recent developments
João F. Mano (2004)
Chondrogenic potential of injectable κ-carrageenan hydrogel with encapsulated adipose stem cells for cartilage tissue-engineering applications.
Elena Geta Popa (2015)
Xenogeneic Acellular Conjunctiva Matrix as a Scaffold of Tissue-Engineered Corneal Epithelium
Haifeng Zhao (2014)
Treatment of rabbit growth plate injuries with oriented ECM scaffold and autologous BMSCs
Wenchao Li (2017)
The effect of cartilage extracellular matrix particle size on the chondrogenic differentiation of bone marrow mesenchymal stem cells.
Chenjun Zhai (2019)
Coatings: Bonelike Apatite via Biodegradable Polymer-Nucleated
Albertina Lima de Oliveira (2015)
Development of a 3D Tissue Engineered Skeletal Muscle and Bone Co-culture System.
Nicholas M Wragg (2019)
Effects of nanobarium titanate on physical and mechanical properties of poly(methyl methacrylate) denture base nanocomposites
Nidal Wanis Elshereksi (2020)
Alkaline treatments to render starch-based biodegradable polymers self-mineralizable.
Isabel B Leonor (2007)
Sodium silicate gel as a precursor for the in vitro nucleation and growth of a bone-like apatite coating in compact and porous polymeric structures.
Ana L. Oliveira (2003)
Novel polymeric systems based on natural materials : development and biological performance
Sandra Milena Luna Osório (2007)
Fabrication and In Vitro Study of Tissue-Engineered Cartilage Scaffold Derived from Wharton's Jelly Extracellular Matrix
Tongguang Xiao (2017)
Periodate oxidized hyaluronic acid-based hydrogel scaffolds for tissue engineering applications.
Ashiq Hussain Pandit (2019)
Molecularly Dispersed Hydroxyapatite Polymer Nanocomposites
Otto C. Wilson (2012)
Cell delivery systems using alginate--carrageenan hydrogel beads and fibers for regenerative medicine applications.
Elena Geta Popa (2011)
Scaffolds for tissue engineering and 3D cell culture.
Eleonora Carletti (2011)
See more
Semantic Scholar Logo Some data provided by SemanticScholar