← Back to Search
Synthesis Of A Fluorescent Chitosan Derivative And Its Application For The Study Of Chitosan-mucin Interactions
R. Qaqish, M. Amiji
Published 1999 · Chemistry
Download PDFAnalyze on Scholarcy
Abstract Fluorescein isothiocyanate-labeled chitosan was synthesized as a macromolecular fluorophore to investigate the molecular mechanism of interactions between chitosan and mucin by the fluorescence polarization method. The percent change in polarization upon chitosan–mucin association was measured as a function of the molecular weight of chitosan, and the pH and ionic strength of the medium. Dextran sulfate and albumin were used as positive and negative controls of polyelectrolyte complexation, respectively. The degree of polarization increased significantly with increasing mucin/chitosan (molecular weight, 750 000 daltons) molar ratio in 0.10 M acetic acid to suggest that there was multivalent binding between the two polymers. In contrast, only one dextran sulfate molecule was able to bind with the chitosan molecule. Albumin, having a net positive charge at a pH of 2.9, did not bind with chitosan. There was a possibility of multivalent association between high molecular weight chitosan and mucin as compared to univalent association with low molecular weight chitosan. The results of pH and ionic strength studies suggest that, in addition to electrostatic interactions between d -glucosamine residue of chitosan and the sialic acid residue of mucin, there could be other attractive forces involved in chitosan–mucin binding such as hydrogen bonding and/or hydrophobic interaction.
This paper references
Fluorescence polarization analysis of protein-DNA and protein-protein interactions.
J. Lundblad (1996)
Physico-chemical properties of water insoluble polymers important to mucin/epithelial adhesion☆
H. Park (1985)
Surface, interfacial and molecular aspects of polymer bioadhesion on soft tissues
N. Peppas (1985)
Stereoselective Systemic Disposition of Ibuprofen Enantiomers in the Dog
Hae-Young Ahn (2004)
pH-dependent interactions between adsorbed chitosan layers
P. Claesson (1992)
Polyanionic Polymers in Bio- and Mucoadhesive Drug Delivery
S. S. Leung (1992)
Structure Elucidation and Thermospray High-Performance Liquid Chromatography/Mass Spectroscopy (HPLC/MS) of the Microbial and Mammalian Metabolites of the Antimalarial Arteether
C. Hufford (2004)
Bioadhesive drug delivery systems. I. Characterisation of mucoadhesive properties of systems based on glyceryl mono-oleate and glyceryl monolinoleate.
L. S. Nielsen (1998)
Bioadhesive polymers for the peroral delivery of peptide drugs
H. L. Luessen (1994)
Preliminary studies of oral mucosal delivery of peptide drugs
M. Veillard (1987)
A new protease activity assay using fluorescence polarization.
R. Bolger (1994)
Bioadhesive polymers as platforms for oral-controlled drug delivery: method to study bioadhesion
K. Park (1984)
Chitosans as absorption enhancers of poorly absorbable drugs. 3: Influence of mucus on absorption enhancement.
N. G. Schipper (1999)
Polarisation de la lumière de fluorescence. Vie moyenne des molécules dans l'etat excité
F. Perrin (1926)
Fluorescence polarization in immunochemistry.
W. Dandliker (1970)
Bioadhesive polymers as platforms for oral controlled drug delivery II: synthesis and evaluation of some swelling, water-insoluble bioadhesive polymers.
H. S. Ch'ng (1985)
Rotational Brownian motion and polarization of the fluorescence of solutions.
G. Weber (1953)
3 – Serum Albumin
T. Peters (1975)
Applications and Properties of Chitosan
Qiangliang Li (1992)
Fluorescence polarization to evaluate the fluidity of natural and reconstituted membranes.
E. M. Sorensen (1988)
Some Applications of Fluorimetry to Synthetic Polymer Studies
H. Morawetz (1979)
Mucus physiology and pathology
The Determination of the Degree of Acetylation of Chitosans by Spectrophotometry
R. Muzzarelli (1986)
Polymeric structure of pig small-intestinal mucus glycoprotein. Dissociation by proteolysis or by reduction of disulphide bridges.
M. Mantle (1981)
Application of fluorescence spectroscopy for determining the structure and function of proteins.
W. Jiskoot (1995)
Chitosan--as a biomaterial.
T. Chandy (1990)
Composition and functioin of human cervical mucus.
D. Wolf (1980)
Fluorescence polarization study of the poly(acrylic acid)/poly(ethylene oxide) interpolymer complex in aqueous solution
J. J. Heyward (1989)
This paper is referenced by
Handbook of Encapsulation and Controlled Release
M. Mishra (2015)
Precise derivatization of structurally distinct chitosans with rhodamine B isothiocyanate
Odette Ma (2008)
Mucoadhesive property and biocompatibility of methylated N-aryl chitosan derivatives
Warayuth Sajomsang (2009)
Amoxicillin Loaded Chitosan–Alginate Polyelectrolyte Complex Nanoparticles as Mucopenetrating Delivery System for H. Pylori
S. Arora (2011)
Advances in mucoadhesion and mucoadhesive polymers.
V. Khutoryanskiy (2011)
Stabilization and Release Effects of Pluronic F 127 in Protein Drug Delivery
J. England (1999)
Hydrocaffeic acid-chitosan nanoparticles with enhanced stability, mucoadhesion and permeation properties.
G. M. Soliman (2014)
Development of a chitosan-based adhesive. Application to wood bonding
A. K. Patel (2013)
Bioavailability of nanoparticles in nutrient and nutraceutical delivery
E. Acosta (2009)
Investigating the role of chitin deacetylation in Magnaporthe oryzae
Ivey Geoghegan (2015)
The effect of particle structure of chitosan-coated liposomes and type of chitosan on oral delivery of calcitonin
J. Thongborisute (2006)
Layer-by-Layer Assembled Thin Film of Albumin Nanoparticles for Delivery of Doxorubicin
Vaishalchi Mohanta (2012)
A fluorescence spectroscopy and fluorescence anisotropy investigation of the heteroaggregation of alginate microparticles and chitosan nanoparticles
Chinmay Pathak (2015)
Design molecular topology for wet-dry adhesion.
Jiawei Yang (2019)
Quorum Sensing based Biofouling Control in Membrane Bioreactor for Advanced Water Treatment
Kyung-Min Yeon (2009)
Tuneable Chitosan Particles with Potential Forensic and Pharmaceutical Applications
Ezzeddin M A Hejjaji (2018)
Immersion delivery of plasmid DNA. II. A study of the potentials of a chitosan based delivery system in rainbow trout (Oncorhynchus mykiss) fry.
K. Romøren (2002)
Enhancing the in vitro anticancer activity of albendazole incorporated into chitosan-coated PLGA nanoparticles.
Bong-Seok Kang (2017)
Buccal Delivery Systems
Jinsong Hao (2003)
In vitro evaluation of the mucoadhesive properties of polysaccharide-based nanoparticulate oral drug delivery systems.
Siwar Chayed (2007)
Preparation and Characterization of the Fluorescent Chitosan Nanoparticle Probe
Jiayin Zhao (2006)
Facile synthesis of acyl chitosan isothiocyanates and their application to porphyrin-appended chitosan derivative.
Masaya Shibano (2014)
Chitosan against cutaneous pathogens
J. Champer (2013)
Electroformation of giant vesicles from an inverse phase precursor.
O. Mertins (2009)
UNIVERSIDADE ESTADUAL DE CAMPINAS FACULDADE DE TECNOLOGIA ISADORA DE SOUZA LOPES CHITOSAN NANOPARTICLES FORMATION AND APPLICATION IN THE DE- EMULSIFICATION OF DAIRY WASTES FORMAÇÃO DE NANOPARTÍCULAS DE QUITOSANA E APLICAÇÃO NA DE- EMULSIFICAÇÃO DE EFLUENTES DE LATICÍNIOS LIMEIRA 2018 ISADORA
FORMAÇÃO DE NANOPARTÍCULAS (2018)
Antimicrobial Activity Of Lincomycin and Gentamicin delivered from Chitosan and Chitosan-Gelatin Matrices
Hanaa Jaafer jabaar Al –kabee (2008)
Investigation of the Interaction between Mucins and-Lactoglobulin under 2 Tribological Stress
Hilal Yilmaz Celebioglu (2019)
One-step ultrasound producing O/W emulsions stabilized by chitosan particles.
A. L. R. Costa (2018)
Evaluating the Effects of Vacuum Tumbling with Chitosan Nanoparticles and Water-Soluble Chitosan on the Shelf Life of Cryogenically Frozen Shrimp
Alexander Chouljenko (2015)
Solid lipid nanoparticles prepared by coacervation method as vehicles for ocular cyclosporine
L. Battaglia (2012)
Evaluation of the Factors Influencing Stomach‐specific Delivery of Antibacterial Agents for Helicobacter pylori Infection
S. Shah (1999)
Preparation and photophysical behaviors of fluorescent chitosan bearing fluorescein: Potential biomaterial as temperature/pH probes
Xiaolin Guan (2007)See more