Online citations, reference lists, and bibliographies.
← Back to Search

Investigation On A Novel Core-coated Microspheres Protein Delivery System.

S. Zhou, X. Deng, X. Li
Published 2001 · Medicine, Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
Among the different approaches to achieve protein delivery, the use of polymers, specifically biodegraded, holds great promise. In this work, a new microsphere delivery system composed of alginate microcores surrounded by a biodegradable poly-DL-lactide-poly(ethylene glycol (PELA) was designed to improve the loading efficiency and stability of proteins. Alginate was solidified by calcium (MS-1), polylysine (MS-2) and chitosan (MS-3), respectively, to form different microcores. Human Serum Albumin (HSA), used as a model protein, was efficiently entrapped within the alginate microcores using a high-speed stirrer and then microencapsulated into PELA copolymer using a w/o/w solvent extraction method. DSC analysis of the microspheres revealed the efficient encapsulation of the alginate microcores, while the microcores were dispersed in the PELA matrix. SDS-PAGE results showed that HSA kept its structural integrity during encapsulation and release procedure. Microspheres were characterized in terms of morphology, size, loading efficiency, in vitro degradation and protein release. The degradation profiles were characterized by measuring the loss of microsphere mass, the decrease of polymer intrinsic viscosity and the reduction of PEG content of PELA coat. The release profiles were investigated from the measurement of protein presented in the release medium at various intervals. The results were that the degradation rate of these core-coated microspheres was MS-2>MS-1>MS-3. The extent of burst release from the core-coated microspheres in the initial protein release was lower than the 27% burst release from the conventional microspheres. In conclusion, the work presents a new approach for macromolecular drugs (such as protein, peptide drugs) delivery. The core-coated microspheres system may have potential use as a carrier for drugs that are poorly absorbed after oral administration.
This paper references
10.1002/1097-4628(20001003)78:1<140::AID-APP180>3.0.CO;2-P
In vitro degradation and release profiles of poly-DL-lactide-poly(ethylene glycol) microspheres with entrapped proteins
X. Li (2000)
10.1515/9783050077338-026
Y
E. M. S. J. xviii (1824)
10.1016/0168-3659(89)90047-3
The acceleration of degradation-controlled drug delivery from polyester microspheres
Y. Cha (1989)
Modern chemical reagent poly - DL - lactide - poly ( ethylene glycol ) microspheres encap - manual
J. J. Xu (1999)
10.1016/0168-3659(87)90080-0
Biodegradable implant composites for local therapy
I. Kaetsu (1987)
Puclakkainen et al., Calcium–alginate beads for the oral delivery of transforming growth factor-B (TGF-B ): stabilization of TGF-B by the 1 1 addition of polyacrylic acid within acid-treated beads
R. J. Mumber (1994)
10.1515/9783111548050-024
M
M. Sankar (1824)
10.1002/JPS.2600781117
Spherical agglomerates of water-insoluble drugs.
R. Bodmeier (1989)
10.1016/0168-3659(94)90082-5
Biodegradable microspheres as depot system for patenteral delivery of peptide drugs
R. Mehta (1994)
10.1016/S0140-6736(05)61503-6
T
D. Davies (1998)
Stability and release of bovine serum nate beads for oral delivery of insulin
T. Chandy
10.1016/0168-3659(94)90030-2
Calcium-alginate beads for the oral delivery of transforming growth factor-β1 (TGF-β1) : stabilization of TGF-β1 by the addition of polyacrylic acid within acid-treated beads
R. Mumper (1994)
10.5040/9781474284028.0024
S
A. Kumar (1824)
10.1016/0168-3659(94)00084-8
Importance of in vitro experimental conditions on protein release kinetics, stability and polymer degradation in protein encapsulated poly (d,l-lactic acid-co-glycolic acid) microspheres
T. Park (1995)
Alginate – Pection - poly - L - lysine par - peptide drugs
X. H. Li
Protein release from poly(lactic-co-glycolic acid) microspheres: protein stability problems.
W. Lu (1995)
Protein release from Poly ( lactic - co - gly - of protein – dye binding
T. G. Park Lu (1976)
Preparation of poly-DL-lactide-poly(ethylene glycol) microspheres encapsulating human serum albumin
X. H. Li (1999)
10.1016/S0168-3659(00)00235-2
Influence of process parameters on the protein stability encapsulated in poly-DL-lactide-poly(ethylene glycol) microspheres.
X. Li (2000)
Improved and release profiles of poly - DL - lactide - poly ( ethylene glycol ) immunogenicity of a core - coated tetanus toxoid delivery microspheres with entrapped proteins
S. P. Schwendeman Tobio (2000)
10.1016/0168-3659(93)90070-L
A formulation method using D,L-lactic acid oligomer for protein release with reduced initial burst
Y. Tabata (1993)
10.1248/CPB.44.235
Instability of bovine insulin in poly(lactide-co-glycolide) (PLGA) microspheres.
T. Uchida (1996)
10.1002/(SICI)1097-4628(19960314)59:11<1795::AID-APP16>3.0.CO;2-T
Chitosan/calcium–alginate beads for oral delivery of insulin
P. Hari (1996)
10.1016/S0378-5173(97)00339-6
A novel approach to stabilization of protein drugs in poly(lactic-co-glycolic acid) microspheres using agarose hydrogel
N. Wang (1998)
10.1016/S0168-3659(96)01511-8
Stability and release of bovine serum albumin encapsulated within poly(d,l-lactide-co-glycolide) microparticles
G. Crotts (1996)
10.1002/POL.1990.140281303
Synthesis and characterization of block copolymers from D, L‐lactide and poly(ethylene glycol) with stannous chloride
X. Deng (1990)
10.1016/0168-3659(95)00098-4
Additive effect of chondroitin sulfate and chitosan on drug release from calcium-induced alginate gel beads
Y. Murata (1996)
10.1016/S0264-410X(99)00313-8
Improved immunogenicity of a core-coated tetanus toxoid delivery system.
M. Tobío (1999)
10.1248/CPB.36.1095
A new technique to efficiently entrap leuprolide acetate into microcapsules of polylactic acid or copoly(lactic/glycolic) acid.
Y. Ogawa (1988)
10.1016/S0168-3659(98)00147-3
Optimization of preparative conditions for poly-DL-lactide- polyethylene glycol microspheres with entrapped Vibrio cholera antigens.
X. Deng (1999)
10.1016/0142-9612(96)84680-1
Alginate polycation microcapsules. I. Interaction between alginate and polycation.
B. Thu (1996)
10.1211/0022357991772259
Alginate‐Pectin‐Poly‐L‐lysine Particulate as a Potential Controlled Release Formulation
P. Liu (1999)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)



This paper is referenced by
10.1177/0883911506066930
Perspectives on: Chitosan Drug Delivery Systems Based on their Geometries
E. Denkbaş (2006)
10.1080/10717540802321727
Preparation of Biodegradable Polycaprolactone/Poly (ethylene glycol)/Polycaprolactone (PCEC) Nanoparticles
W. J. Jia (2008)
10.3928/01477447-20100625-15
Evaluation of a novel osteoporotic drug delivery system in vitro: alendronate-loaded calcium phosphate cement.
Zhao Jin-dong (2010)
10.1016/j.jconrel.2013.02.013
Polyalkylcyanoacrylates as in situ formed diffusion barriers in multimaterial drug carriers.
Christian Wischke (2013)
10.1016/j.ijbiomac.2017.03.181
Systematic investigation of fabrication conditions of nanocarrier based on carboxymethyl chitosan for sustained release of insulin.
X. Bai (2017)
Development of food polymer-based colloidal delivery systems for nutraceuticals
Yangchao Luo (2012)
10.3109/02652048.2015.1046517
Chitosan nanoparticles as adenosine carriers
M. Kazemzadeh-Narbat (2015)
10.7314/APJCP.2012.13.12.6363
Preparation of microspheres encapsulating a recombinant TIMP-1 adenovirus and their inhibition of proliferation of hepatocellular carcinoma cells.
Dong Zhou Xia (2012)
10.1007/s13197-019-03955-8
Enhanced stability of C-phycocyanin colorant by extrusion encapsulation
H. Pradeep (2019)
10.1155/2020/2785304
Effectual Anticancer Potentiality of Loaded Bee Venom onto Fungal Chitosan Nanoparticles
Adel I. Alalawy (2020)
Design and Evaluation of Chitosan Nanoparticles as Novel Drug Carriers for the Delivery of Donepezil
Dinesh Raja Azalea (2012)
10.1016/j.ijbiomac.2014.11.014
Immunopotentiating nano-chitosan as potent vaccine carter for efficacious prophylaxis of filarial antigens.
B. Malathi (2015)
10.1016/J.JMMM.2011.05.045
Preparation of magnetic polylactic acid microspheres and investigation of its releasing property for loading curcumin
Fengxia Li (2011)
10.1007/S11274-006-9205-6
Immobilization of Laccase by Alginate–Chitosan Microcapsules and its Use in Dye Decolorization
L. Lu (2007)
10.1016/j.colsurfb.2011.06.003
Preparation and characterization novel polymer-coated magnetic nanoparticles as carriers for doxorubicin.
F. Li (2011)
10.22092/ARI.2016.103899
PREPARING AND CHARACTERIZING CHITOSAN NANOPARTICLES CONTAINING HEMISCORPIUS LEPTURUS SCORPION VENOM AS AN ANTIGEN DELIVERY SYSTEM
N. M. Dounighi (2012)
10.1002/APP.35527
Valproate‐Loaded hydrogel nanoparticles: Preparation and characterization
M. Hamidi (2011)
10.11569/wcjd.v13.i1.20
Construction of microsphere encapusulating recombinant adenovirus with antisense multidrug resistance-associated protein gene and its effect on hepatocellular carcinoma
Shao-hong Yu (2005)
10.1007/978-1-62703-550-7_21
Encapsulation of cells in alginate gels.
P. Serrano Sanchez (2013)
10.16966/2470-3206.109
Encapsulation of Vitamin C by A Double- Layer Zein/Chitosan Structure with Improved Stability and Controlled Release
Fangyuan Dong (2016)
10.1007/s10856-012-4644-0
Calcium alginate/dextran methacrylate IPN beads as protecting carriers for protein delivery
Giorgia D’Arrigo (2012)
10.1016/j.stam.2005.03.017
Hydrophilized poly(lactide-co-glycolide) nanoparticles with core/shell structure for protein delivery
Sung Kyun Han (2005)
10.1002/jbm.b.31085
Electrospraying: a facile technique for synthesis of chitosan-based micro/nanospheres for drug delivery applications.
Neha Arya (2009)
FORMULATION AND EVALUATION OF METFORMIN HYDROCHLORIDE BEADS BY IONOTROPIC GELATION TECHNIQUE
Shri G. M. Bilakhia (2012)
10.1016/j.colsurfb.2008.07.020
Chitosan-coated magnetic nanoparticles as carriers of 5-fluorouracil: preparation, characterization and cytotoxicity studies.
L. Zhu (2009)
10.1021/bm200576h
Production and evaluation of dry alginate-chitosan microcapsules as an enteric delivery vehicle for probiotic bacteria.
M. Cook (2011)
10.3109/03639045.2014.991400
Design, characterization, and evaluation of intranasal delivery of ropinirole-loaded mucoadhesive nanoparticles for brain targeting
Omidreza Jafarieh (2015)
10.1002/mabi.201600328
Osmogen-Mediated One-Step Technique of Fabricating Hollow Microparticles for Encapsulation and Delivery of Bioactive Molecules.
Sharad Kharel (2017)
10.1016/J.EJPB.2007.10.016
Release pattern and structural integrity of lysozyme encapsulated in core-sheath structured poly(DL-lactide) ultrafine fibers prepared by emulsion electrospinning.
Y. Yang (2008)
10.1016/j.nano.2009.04.001
Chitosan nanoparticles as a new delivery system for the anti-Alzheimer drug tacrine.
B. Wilson (2010)
10.3109/10837450.2010.513989
Reduction in burst release after coating poly(D,L-lactide-co-glycolide) (PLGA) microparticles with a drug-free PLGA layer
A. Ahmed (2012)
10.1007/S11434-007-0127-Y
Water-soluble chitosan nanoparticles as a novel carrier system for protein delivery
W. Chun (2007)
See more
Semantic Scholar Logo Some data provided by SemanticScholar