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Development Of Intestinal Cell Culture Models For Drug Transport And Metabolism Studies
A. Quaroni, J. Hochman
Published 1996 · Biology
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Abstract Cell culture models offer many advantageous features for the analysis of drug transport and drug metabolism. From a basic research perspective, these systems offer the potential for manipulating the environment or cellular properties as a means to address mechanistic questions. From a drug discovery perspective, cell culture models can be used to expedite identification of compounds with favorable pharmacokinetic properties, and to evaluate structure-absorption/metabolism relationships. In this review, we will use the intestinal epithelium as an example for discussing issues associated with the development of new cell culture models for evaluating drug metabolism. Specifically, we will discuss biological properties of the intestinal epithelium and address biological and practical consideration in the application of tumor cell lines, short-term primary cultures, stem-like cell cultures, and oncogene immortalized cells as approaches to establishing models for the intestinal epithelium.
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Biochemical characterization of collagens synthesized by intestinal epithelial cell cultures.
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Cooperative tumorigenic effects of germline mutations in Rb and p53
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The posttranslational processing of sucrase-isomaltase in HT-29 cells is a function of their state of enterocytic differentiation
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Anchoring and biosynthesis of stalked brush border membrane proteins: glycosidases and peptidases of enterocytes and renal tubuli.
G. Semenza (1986)
Ontogeny of enzymes in the small intestine.
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The molecular genetics of cellular oncogenes.
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Human placental cells transformed by tsA mutants of simian virus 40: a model system for the study of placental functions.
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Distribution of immunoglobulin G receptors in the small intestine of the young rat
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Effect of oncogenic virus on muscle differentiation.
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Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability.
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Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia
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Structure, biochemistry, and assembly of epithelial tight junctions.
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G1 events and regulation of cell proliferation.
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1 – Enterocyte Division and Differentiation
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Establishment of rat fetal liver lines and characterization of their metabolic and hormonal properties: use of temperature-sensitive SV40 virus.
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Transient mosaic patterns of morphological and functional differentiation in the Caco-2 cell line.
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The retinoblastoma tumor suppressor protein.
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Transport of bile acids in a human intestinal epithelial cell line, Caco-2.
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Sorting of endogenous plasma membrane proteins occurs from two sites in cultured human intestinal epithelial cells (Caco-2)
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Study of liver differentiation in vitro
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Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
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Cytotoxicity potential of surfactant mixtures evaluated by primary cultures of rabbit corneal epithelial cells.
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Identification of vitamin D-stimulated alkaline phosphatase in IEC-6 cells, a rat small intestine crypt cell line.
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Human intestinal goblet cells in monolayer culture: characterization of a mucus-secreting subclone derived from the HT29 colon adenocarcinoma cell line.
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Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate.
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Fetal gut mesenchyme induces differentiation of cultured intestinal endodermal and crypt cells.
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Paneth cells of the human small intestine express an antimicrobial peptide gene.
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Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
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Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture
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A novel cell line (Caco-2) for the study of intestinal lipoprotein synthesis.
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The processing of asparagine-linked oligosaccharides in HT-29 cells is a function of their state of enterocytic differentiation. An accumulation of Man9,8-GlcNAc2-Asn species is indicative of an impaired N-glycan trimming in undifferentiated cells.
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Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation.
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2 Structure and Genomic Organization of SV40 and Polyoma Virus
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Characterization of a cyclic AMP-activated Cl-transport pathway in the apical membrane of a human colonic epithelial cell line.
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Human colon cancer cell lines secrete alpha TGF-like activity.
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Functional coupling of tight junctions and microfilaments in T84 monolayers.
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Development of fetal rat intestine in organ and monolayer culture
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Type X collagen gene expression in mouse chondrocytes immortalized by a temperature-sensitive simian virus 40 large tumor antigen
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Identification and characterization of the rat intestinal epithelial cell (IEC-18) interleukin-1 receptor.
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Mesenchyme-dependent differentiation of epithelial progenitor cells in the gut.
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Ulcerative colitis-like disease in mice with a disrupted interleukin-2 gene
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Increased growth adaptability to 5‐fluorouracil and methotrexate of HT‐29 sub‐populations selected for their commitment to differentiation
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Transfection of fetal rat intestinal epithelial cells by viral oncogenes: establishment and characterization of the E1A-immortalized SLC-11 cell line.
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Radioautographic visualization of differences in the pattern of [3H]uridine and [3H]orotic acid incorporation into the RNA of migrating columnar cells in the rat small intestine
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Enterocytic differentiation and glucose utilization in the human colon tumor cell line Caco‐2: Modulation by forskolin
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Conditional enhancement of liver-specific gene transcription.
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Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges.
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Differentiated mammalian cell lines immortalized by temperature-sensitive tumor viruses.
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Postnatal development of corticosteroid-binding globulin: effects of thyroxine.
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Organization of actin, myosin, and intermediate filaments in the brush border of intestinal epithelial cells
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Fibronectin synthesis by epithelial crypt cells of rat small intestine.
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Chapter 20 Establishment and Characterization of Intestinal Epithelial Cell Cultures
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Quantitation of the frequency of immortalization of normal human diploid fibroblasts by SV40 large T-antigen.
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Mechanisms of absorption enhancement and tight junction regulation
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Crypt cell antigens (CCA): new carbohydrate markers for human colon cancer cells.
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Isolation and characterization of the human sucrase-isomaltase gene and demonstration of intestine-specific transcriptional elements.
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Proliferation and differentiation of fetal rat intestinal epithelial cells in primary serum-free culture.
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Efficient transformation of human fibroblasts by adenovirus-simian virus 40 recombinants.
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Growth-regulatory effects of sensory neuropeptides, epidermal growth factor, insulin, and somatostatin on the non-transformed intestinal epithelial cell line IEC-6 and the colon cancer cell line HT 29.
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Symmetric infection of rotavirus on polarized human intestinal epithelial (Caco-2) cells.
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Effects of cytochalasin D on occluding junctions of intestinal absorptive cells: further evidence that the cytoskeleton may influence paracellular permeability and junctional charge selectivity
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Cytotoxic effects and metabolism of benzo[a]pyrene and 7,12-dimethylbenz[a]anthracene in duodenal and ileal epithelial cell cultures.
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The development of the mucous membrane of the œsophagus, stomach and small intestine in the human embryo
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Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2.
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Structure of the adenovirus 2 early mRNAs
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Clonal analysis of intestinal crypt populations in mouse aggregation chimaeras.
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Derivation of conditionally immortalized cell lines containing the Min mutation from the normal colonic mucosa and other tissues of an "Immortomouse"/Min hybrid.
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The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases
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What triggers intestinal cells to move or stay and to proliferate or differentiate?
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Regulation of human liver cytochromes P‐450 in family 3A in primary and continuous culture of human hepatocytes
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Decreased expression of protooncogenes c-fos, c-myc, and c-jun following polyamine depletion in IEC-6 cells.
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Fetal characteristics of small intestinal crypt cells.
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Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line
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Organization of early region 1B of human adenovirus type 2: identification of four differentially spliced mRNAs.
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