Please confirm you are human (Sign Up for free to never see this)
← Back to Search
Protein Biochips: A New And Versatile Platform Technology For Molecular Medicine.
A. Lueking, D. Cahill, S. Muellner
Published 2005 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
The human genome has been sequenced and the challenges of understanding the function of the newly discovered genes have been addressed. High-throughput technologies such as DNA microarrays have been developed for the profiling of gene expression patterns in whole organisms or tissues. Protein arrays are emerging to follow DNA chips as possible screening tools. Here, we review the generation and application of microarray technology to obtain more information on the regulation of proteins, their biochemical functions and their potential interaction partners. Already, a large variety of assays based on antibody-antigen interactions exists. In addition, the medical relevance of protein arrays will be discussed.
This paper references
Preparation and Characterization of Self-Assembled Double-Stranded DNA (dsDNA) Microarrays for Protein:dsDNA Screening Using Atomic Force Microscopy
J. C. O'Brien (2000)
Ribosome display: an in vitro method for selection and evolution of antibodies from libraries.
C. Schaffitzel (1999)
Proteome profiling of bladder squamous cell carcinomas: identification of markers that define their degree of differentiation.
M. Ostergaard (1997)
Experimental autoimmune myasthenia gravis in naive non-obese diabetic (NOD/LtJ) mice: susceptibility associated with natural IgG antibodies to the acetylcholine receptor.
F. Quintana (2003)
Allergen microarray: comparison of microarray using recombinant allergens with conventional diagnostic methods to detect allergen‐specific serum immunoglobulin E
B. Jahn-Schmid (2003)
Printing proteins as microarrays for high-throughput function determination.
G. MacBeath (2000)
Establishing the yeast Saccharomyces cerevisiae as a system for expression of human proteins on a proteome-scale
C. Holz (2004)
Applications of peptide arrays prepared by the SPOT-technology.
U. Reineke (2001)
The use of recombinant antibodies in proteomics.
Lucy J. Holt (2000)
Analyzing antibody specificity with whole proteome microarrays
G. Michaud (2003)
Immunization with DNA encoding an immunodominant peptide of insulin prevents diabetes in NOD mice.
I. Urbanek-Ruiz (2001)
A system for dual protein expression in Pichia pastoris and Escherichia coli.
A. Lueking (2000)
A micro-scale process for high-throughput expression of cDNAs in the yeast Saccharomyces cerevisiae.
C. Holz (2002)
A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library.
K. Büssow (1998)
Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis.
G. Mahairas (1996)
Generation of Arabidopsis protein chips for antibody and serum screening
B. Kersten (2004)
Combination of gene delivery and DNA vaccination to protect from and reverse Th1 autoimmune disease via deviation to the Th2 pathway.
H. Garren (2001)
Sol-gel encapsulated enzyme arrays for high-throughput screening of biocatalytic activity.
C. Park (2002)
Proteome-scale purification of human proteins from bacteria
P. Braun (2002)
From genes to proteins: High‐throughput expression and purification of the human proteome
Joanna S. Albala (2000)
Protein microchips: use for immunoassay and enzymatic reactions.
P. Arenkov (2000)
A human cDNA expression library in yeast enriched for open reading frames.
C. Holz (2001)
Peptide chips for the quantitative evaluation of protein kinase activity
Benjamin T. Houseman (2002)
Aptamers as therapeutic and diagnostic agents.
E. Brody (2000)
ICln, a Novel Integrin αIIbβ3-Associated Protein, Functionally Regulates Platelet Activation*
Deirdre Larkin (2004)
Quantifying DNA–protein interactions by double-stranded DNA arrays
M. Bulyk (1999)
Identification of barley CK2alpha targets by using the protein microarray technology.
A. Kramer (2004)
C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression
J. Reboul (2003)
Suppressive immunization with DNA encoding a self-peptide prevents autoimmune disease: modulation of T cell costimulation.
P. J. Ruiz (1999)
A Mitogen-activated Protein Kinase-dependent Signaling Pathway in the Activation of Platelet Integrin αIIbβ3 *
Z. Li (2001)
Profiling humoral autoimmune repertoire of dilated cardiomyopathy (DCM) patients and development of a disease‐associated protein chip
Sabine C. Horn (2006)
A human cDNA library for high-throughput protein expression screening.
K. Büssow (2000)
A Sequence within the Cytoplasmic Tail of GpIIb Independently Activates Platelet Aggregation and Thromboxane Synthesis*
G. Stephens (1998)
Detection of multiple allergen-specific IgEs on microarrays by immunoassay with rolling circle amplification.
S. Wiltshire (2000)
Identification and characterization of potential new therapeutic targets in inflammatory and autoimmune diseases.
U. Mangold (1999)
Identification and characterization of positive regulatory elements in the human glyceraldehyde 3-phosphate dehydrogenase gene promoter.
T. Aki (1997)
Microarrayed allergens for IgE profiling.
K. Deinhofer (2004)
Dihydroorotate Dehydrogenase Is a High Affinity Binding Protein for A77 1726 and Mediator of a Range of Biological Effects of the Immunomodulatory Compound (*)
R. A. Williamson (1995)
Designing isoform-specific peptide disruptors of protein kinase A localization
Lora L Burns-Hamuro (2003)
GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes.
A. J. Walhout (2000)
A dual-expression vector allowing expression in E. coli and P. pastoris, including new modifications.
A. Lueking (2003)
Analysis of yeast protein kinases using protein chips
H. Zhu (2000)
CDNAs for functional genomics and proteomics: the German Consortium.
S. Wiemann (2003)
Drug discovery: a historical perspective.
J. Drews (2000)
An Array Immunosensor for Simultaneous Detection of Clinical Analytes
Chris A. Rowe (1999)
The FLEXGene repository: exploiting the fruits of the genome projects by creating a needed resource to face the challenges of the post-genomic era.
L. Brizuela (2002)
Genome-scale cloning and expression of individual open reading frames using topoisomerase I-mediated ligation.
J. Heyman (1999)
Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling.
C. Gutjahr (2005)
Site-specific peptide immobilization strategies for the rapid detection of kinase activity on microarrays.
M. Uttamchandani (2004)
Autoantigen microarrays for multiplex characterization of autoantibody responses
W. Robinson (2002)
Molecular profiling of the immune response in colon cancer using protein microarrays: Occurrence of autoantibodies to ubiquitin C‐terminal hydrolase L3
M. Nam (2003)
High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris.
M. Boettner (2002)
Protein microarrays guide tolerizing DNA vaccine treatment of autoimmune encephalomyelitis
W. Robinson (2003)
Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatment
R. Hiller (2002)
Solid supports for microarray immunoassays
W. Kusnezow (2003)
Aptamers: an emerging class of molecules that rival antibodies in diagnostics.
S. Jayasena (1999)
Functional immunomics: Microarray analysis of IgG autoantibody repertoires predicts the future response of mice to induced diabetes
F. Quintana (2004)
A Nonredundant Human Protein Chip for Antibody Screening and Serum Profiling*
A. Lueking (2003)
The use of aptamers in large arrays for molecular diagnostics.
E. Brody (1999)
High-throughput screening of surface displayed gene products.
G. Walter (2001)
Protein microarrays for gene expression and antibody screening.
A. Lueking (1999)
Cell-free protein expression and functional assay in nanowell chip format.
Philipp Angenendt (2004)
Differential analysis of phosphorylated proteins in resting and thrombin-stimulated human platelets
Katrin Marcus (2003)
A microchip-based enzyme assay for protein kinase A.
C. Cohen (1999)
This paper is referenced by
Chapter 9 – Clinical Proteomics and Molecular Pathology
Lance A. Liotta (2009)
Survey of the year 2005 commercial optical biosensor literature
R. Rich (2006)
SPR imaging-based monitoring of caspase-3 activation.
K. Park (2008)
Technology Insight: pharmacoproteomics for cancer—promises of patient-tailored medicine using protein microarrays
J. Wulfkuhle (2006)
Protein Microarray Technologies for Detection and Identification of Bacterial and Protein Analytes
C. Wingren (2008)
Microarray technology in obstetrics and gynecology: A guide for clinicians
K. Ward (2006)
Protein Expression In Prostate Cancer
H. Lexander (2006)
Do There Exist Innovative Perspectives in Tissue Engineering-Based Ligament Reconstruction?
Gundula Schulze Tanzil (2017)
Personalized Diagnosis and Therapy
G€unter M€uller (2015)
Applications of Protein Biochips in Biomedical and Biotechnological Research
Dirk Weinrich (2009)
Optimised 'on demand' protein arraying from DNA by cell free expression with the 'DNA to Protein Array' (DAPA) technology.
R. Schmidt (2013)
Use of Proteomics Analysis for Molecular Precision Approaches in Cancer Therapy
Y. Shen (2008)
Comparative proteomics analysis to annexin B1 DNA and protein vaccination in mice.
Dean Li (2007)
Surface modification of and selective protein attachment to a flexible microarray pattern using atmospheric plasma with a reactive gas.
H. Lee (2010)
Survey of the year 2003 commercial optical biosensor literature
R. Rich (2005)
Solid-Supported Multicomponent Patterned Monolayers†
Daniel Rhinow (2007)
20. Biofunctionalized Surfaces
Marisela Vélez (2013)
Generation of High Density Protein Microarrays by Cell-free in Situ Expression of Unpurified PCR Products *
Philipp Angenendt (2006)
Proteomics: An Overview
I. Lawrance (2005)
Trends in Biosensing and Biosensors
F. Davis (2010)
Nanostructured substrates for high density protein arrays
Frank A. Zoller (2008)
Holographic molecularly imprinted polymers for label-free chemical sensing.
Y. Fuchs (2013)
Protein silencing with Phylomers: a new tool for target validation and generating lead biologicals targeting protein interactions
P. Watt (2006)
Biosensor stabilization using hypersolutes
Elizabeth Loose (2009)
Bulked sample analysis in genetics, genomics and crop improvement
C. Zou (2016)
Micro and nanofabrication of molecularly imprinted polymers.
Marc Bompart (2012)
Proteomics as a route to identification of toxicity targets in environmental toxicology
V. Dowling (2006)
Proteinbiochips in der Biomedizin und Biotechnologie
Dirk Weinrich (2009)
Analysis of organelles within the nervous system: impact on brain and organelle functions
F. Tribl (2008)
A well-based reverse-phase protein array of formalin-fixed paraffin-embedded tissue.
Joon-Yong Chung (2015)
Functional protein microarray technology
Shaohui Hu (2011)
Measuring and interpreting the selectivity of protein kinase inhibitors
Lynette A. Smyth (2009)See more