A Metabolomic Investigation Of Splanchnic Metabolism Using 1H NMR Spectroscopy Of Bovine Blood Plasma
Published 2005 · Chemistry
Abstract This is the first study introducing an NMR-based metabolomic approach to elucidate metabolism in the portal-drained viscera and liver. High-resolution 1 H NMR spectroscopy was performed on blood plasma obtained from the mesenteric artery, the hepatic portal vein and the hepatic vein of steers before and after interruminal infusion of short-chain fatty acids (SCFA). Partial least squares regression discriminant analysis (PLS-DA) on 1 H NMR spectra obtained for arterial and portal plasma samples revealed increases in the intensities of spectral regions that could mostly be assigned to the methyl group of propionate, β-hydroxybutyrate as well as acetate in portal plasma samples compared with arterial plasma samples. PLS-DA on portal and hepatic plasma samples showed decreases in the intensities of spectral regions corresponding to the regions where methyl and methylene groups of n -butyrate, propionate, iso -butyrate, valerate, iso -valerate as well as lactate are reported to have chemical shifts. In addition, spectral differences likely to reflect a hepatic excretion of β-hydroxybutyrate and glucose were observed between portal and hepatic samples. All findings were supported by traditional biochemical analyses. Consequently, the present study clearly demonstrated the great potential of the NMR-based metabolomic approach for elucidating splanchnic metabolism. In addition, prediction of metabolite concentrations from the 1 H NMR spectra obtained on plasma samples was investigated using PLS, which in relation to use 1 H NMR spectra quantitatively revealed promising results with R -values in the region of 0.87–0.98.