← Back to Search
Hollow And Degradable Polyelectrolyte Nanocapsules For Protein Drug Delivery.
Shujun Shu, Chunyang Sun, X. Zhang, Z. Wu, Zhen Wang, C. Li
Published 2010 · Materials Science, Medicine
Download PDFAnalyze on Scholarcy
Biodegradable hollow capsules encapsulating protein drugs were prepared via layer-by-layer assembly of water-soluble chitosan and dextran sulfate on protein-entrapping amino-functionalized silica particles and the subsequent removal of the silica. In order to enhance the encapsulated efficiency and decrease its burst release, we designed this new system to fulfill these two goals. Bovine serum albumin (BSA), which was used as model protein, was entrapped in the nanocapsules. This system demonstrated a good capacity for the encapsulation and loading of BSA. The burst release was decreased to less than 10% in phosphate-buffered saline within 2h. No significant conformation change was noted from the released BSA in comparison with native BSA by using circular dichroism spectroscopy. Cell viability study suggested that the nanocapsules had good biocompatibility. The drug release kinetics mechanism is Fickian diffusion. These kinds of novel composite nanocapsules may offer a promising delivery system for water-soluble proteins and peptides.
This paper references
Chitosan microspheres prepared by spray drying.
P. He (1999)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
Chitosans as Absorption Enhancers for Poorly Absorbable Drugs 2: Mechanism of Absorption Enhancement
N. G. Schipper (2004)
Effect of Chitosan on Epithelial Cell Tight Junctions
J. Smith (2004)
The drug encapsulation efficiency, in vitro drug release, cellular uptake and cytotoxicity of paclitaxel-loaded poly(lactide)-tocopheryl polyethylene glycol succinate nanoparticles.
Z. Zhang (2006)
Bovine serum albumin adsorption in large pore amine functionalized mesoporous silica
S. Qiao (2007)
Preparation and modification of N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride nanoparticle as a protein carrier.
Y. Xu (2003)
Preparation of nanoparticles composed of chitosan/poly-gamma-glutamic acid and evaluation of their permeability through Caco-2 cells.
Y. Lin (2005)
Development and characterization of new insulin containing polysaccharide nanoparticles.
B. Sarmento (2006)
Gastrointestinal Uptake of Biodegradable Microparticles: Effect of Particle Size
M. P. Desai (2004)
Directed self-assembly of functionalized silica nanoparticles on molecular printboards through multivalent supramolecular interactions.
Venkataramanan Mahalingam (2004)
Oral vaccine delivery.
G. Russell-Jones (2000)
Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery.
Yu-Hsin Lin (2007)
Computed circular dichroism spectra for the evaluation of protein conformation.
N. Greenfield (1969)
Analysis of Fickian and non-Fickian drug release from polymers.
N. Peppas (1985)
Preparation and self-assembly of carboxylic acid-functionalized silica.
Y. An (2007)
Chitosans as absorption enhancers of poorly absorbable drugs. 3: Influence of mucus on absorption enhancement.
N. G. Schipper (1999)
Preparation of insulin nanoparticles and their encapsulation with biodegradable polyelectrolytes via the layer-by-layer adsorption.
Y. Fan (2006)
Preparation of Biodegradable Hollow Nanocapsules by Silica Template Method
Y. Itoh (2004)
Micellar Nanocontainers Distribute to Defined Cytoplasmic Organelles
R. Savić (2003)
Enzymatic Hydrolysis of a Layer-by-Layer Assembly Prepared from Chitosan and Dextran Sulfate
T. Serizawa (2002)
Oral bioavailability of insulin contained in polysaccharide nanoparticles.
B. Sarmento (2007)
Modified Polyelectrolyte Microcapsules as Smart Defense Systems
D. Shchukin (2004)
Recent advances on chitosan-based micro- and nanoparticles in drug delivery.
S. Agnihotri (2004)
Specific and non-specific bioadhesive particulate systems for oral delivery to the gastrointestinal tract.
Melamine formaldehyde core decomposition as the key step controlling capsule integrity : Optimizing the polyelectrolyte capsule fabrication
C. Gao (2002)
Controlled growth of monodisperse silica spheres in the micron size range
W. Stoeber (1968)
Flocculation of Latex Particles of Varying Surface Charge Densities by Chitosans
M. Ashmore (2001)
A model of dissolution-controlled solute release from porous drug delivery polymeric systems.
N. Peppas (1983)
Adsorption and desorption of lysozyme on nano-sized magnetic particles and its conformational changes.
Z. Peng (2004)
Degradable polyelectrolyte capsules filled with oligonucleotide sequences.
A. Zelikin (2006)
This paper is referenced by
Regimes of electrostatic collapse of a highly charged polyelectrolyte in a poor solvent.
Anvy Moly Tom (2017)
In situ forming reduction-sensitive degradable nanogels for facile loading and triggered intracellular release of proteins.
Wei Chen (2013)
Stabilization and Controlled Release of Invertase Through the Addition of Trehalose in Wet and Dried Alginate-Chitosan Beads
Patricio R Santagapita (2015)
Development of antiviral gene therapy for Monodon baculovirus using dsRNA loaded chitosan-dextran sulfate nanocapsule delivery system in Penaeus monodon post-larvae.
D. Ramesh kumar (2016)
Templated globules--applications and perspectives.
Francesca Cuomo (2014)
Development of CS-TPP-dsRNA nanoparticles to enhance RNAi efficiency in the yellow fever mosquito, Aedes aegypti
Ramesh Kumar Dhandapani (2019)
Hemocompatibility evaluation in vitro of methoxy polyethyleneglycol-polycaprolactone copolymer solutions.
Q. Hu (2016)
Facile fabrication of double-walled polymeric hollow spheres with independent temperature and pH dual-responsiveness for synergetic drug delivery
N. Cheng (2016)
Small intestine- and colon-specific smart oral drug delivery system with controlled release characteristic.
Jihye Kang (2018)
X-ray-Controlled Bilayer Permeability of Bionic Nanocapsules Stabilized by Nucleobase Pairing Interactions for Pulsatile Drug Delivery.
Hongzhang Deng (2019)
Precise and tunable time-controlled drug release system using layer-by-layer films as erodible coatings.
J. Tian (2020)
Bioresorbable polyelectrolytes for smuggling drugs into cells
S. Jaganathan (2016)
2 - Nanostructured biobased systems for nutrient and bioactive compounds delivery
M. A. Cerqueira (2017)
Design of Bio-nanosystems for Oral Delivery of Functional Compounds
M. Cerqueira (2013)
Loading and protection of hydrophilic molecules into liposome-templated polyelectrolyte nanocapsules.
Francesca Cuomo (2014)
One example on how colloidal nano- and microparticles could contribute to medicine.
Javier Peteiro-Cartelle (2009)
A pH-sensitive controlled dual-drug release from meso-macroporous silica/multilayer-polyelectrolytes coated SBA-15 composites
Q. Jin (2013)
Mesoporous silica nanotubes coated with multilayered polyelectrolytes for pH-controlled drug release.
Yunjie Yang (2010)
Multifunctional Nano/Micro Polymer Capsules as Potential Drug Delivery and Imaging Agents
Haider Sami (2013)
Like-charged protein-polyelectrolyte complexation driven by charge patches.
Cemil Yiğit (2015)
Templated Synthesis of Chitosan Nanocapsules with Controllable Shell Thickness and Porosity
Emma C Goethals (2010)
Polyelectrolyte capsules packaging BSA gels for pH-controlled drug loading and release and their antitumor activity.
H-J Shen (2013)
hitosan / fucoidan multilayer nanocapsules as a vehicle for controlled elease of bioactive compounds
na C. Pinheiroa (2014)
Template-based syntheses for shape controlled nanostructures.
M. Pérez-Page (2016)
Towards the development of a supercritical carbon dioxide spray process to coat solid protein particles
M. Yu (2017)
Multilayered materials based on biopolymers as drug delivery systems
C. Vilela (2017)
Engineering nanolayered particles for modular drug delivery.
S. Correa (2016)
Growth factor release from polyelectrolyte-coated titanium for implant applications.
A. M. Peterson (2014)
Natural oil nanoemulsions as cores for layer-by-layer encapsulation
M. Adamczak (2013)
pH- and sugar-sensitive layer-by-layer films and microcapsules for drug delivery.
K. Sato (2011)
Preparation and characterization of chitosan nanoparticles as the delivery system for tuberose fragrance
Z. Xiao (2014)
Decoupling the effects of the size, wall thickness, and porosity of curcumin-loaded chitosan nanocapsules on their anticancer efficacy: size is the winner.
Emma C Goethals (2013)See more