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Increased Osteoblast Functions In The Presence Of Hydroxyapatite-coated Iron Oxide Nanoparticles.
N. Tran, T. Webster
Published 2011 · Materials Science, Medicine
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Hydroxyapatite (HA) has been widely used in the biomedical community, especially for orthopedic applications (such as reversing osteoporosis). In order to use HA as injectable nanoparticles that can be directed at will to various locations in the body to treat bone defects, HA was coated onto iron oxide nanoparticles in this study. Specifically, magnetite (Fe3O4) nanoparticles were synthesized and coated with HA. The resulting nanoparticles were treated hydrothermally to control the crystalline properties of the coating. Nanoparticles were characterized via transmission electron microscopy (TEM), dynamic light scattering, X-ray diffraction, Ζeta potential and vibrating sample magnetometry. Nanoparticle uptake by osteoblasts was studied using TEM. Osteoblast density was measured after 1, 3 and 5 days in the presence of Fe3O4 nanoparticles alone and HA-coated Fe3O4 magnetic nanoparticles. Long-term osteoblast experiments demonstrated greater alkaline phosphatase activity, total protein synthesis, collagen synthesis and calcium deposition after 7, 14 and 21 days in the presence of greater concentrations (up to 200 μg ml(-1)) of HA-coated iron oxide nanoparticles. In summary, the results of this study showed that HA-coated magnetic iron oxide nanoparticles should be further studied for various orthopedic applications in which such particles could be injected, their location controlled using an external magnetic source and bone growth promoted.
This paper references
Effect of surface roughness of the titanium alloy Ti-6Al-4V on human bone marrow cell response and on protein adsorption.
D. Deligianni (2001)
The impact of diamond nanocrystallinity on osteoblast functions.
L. Yang (2009)
Increased osteoblast density in the presence of novel calcium phosphate coated magnetic nanoparticles.
R. Pareta (2008)
Specific proteins mediate enhanced osteoblast adhesion on nanophase ceramics.
T. Webster (2000)
MRI after magnetic drug targeting in patients with advanced solid malignant tumors
A. Lemke (2004)
Magnetic nanoparticles as bimodal tools in magnetically induced labelling and magnetic heating of tumour cells: an in vitro study
M. Kettering (2007)
High surface energy enhances cell response to titanium substrate microstructure.
G. Zhao (2005)
Mechanisms of enhanced osteoblast adhesion on nanophase alumina involve vitronectin.
T. Webster (2001)
Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition.
J. Lincks (1998)
Preclinical experiences with magnetic drug targeting: tolerance and efficacy.
A. Lübbe (1996)
Effect of parathyroid hormone (1-34) on fractures and bone mineral density in postmenopausal women with osteoporosis.
R. Neer (2001)
Nanometer surface roughness increases select osteoblast adhesion on carbon nanofiber compacts.
R. L. Price (2004)
The role of nanometer and sub-micron surface features on vascular and bone cell adhesion on titanium.
D. Khang (2008)
Superparamagnetic nanoparticles for biomedical applications: Possibilities and limitations of a new drug delivery system
T. Neuberger (2005)
Targeting and retention of magnetic targeted carriers (MTCs) enhancing intra-arterial chemotherapy
S. Goodwin (1999)
Activation of integrin function by nanopatterned adhesive interfaces.
M. Arnold (2004)
Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion.
B. Keselowsky (2003)
Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, differentiation and detachment strength.
D. Deligianni (2001)
Comparison of the treatment effects of ossein-hydroxyapatite compound and calcium carbonate in osteoporotic females
P. Rüegsegger (2005)
A model for predicting magnetic targeting of multifunctional particles in the microvasculature
E. Furlani (2007)
Clinical trial of microcrystalline hydroxyapatite compound ('Ossopan') in the prevention of osteoporosis due to corticosteroid therapy.
A. Pines (1984)
Deep magnetic capture of magnetically loaded cells for spatially targeted therapeutics.
Zheyong Huang (2010)
Enhanced functions of osteoblasts on nanophase ceramics.
T. Webster (2000)
Clinical experiences with magnetic drug targeting: a phase I study with 4'-epidoxorubicin in 14 patients with advanced solid tumors.
A. Lübbe (1996)
Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, differentiation and detachment
DD Deligianni (2001)
Synthesis and Characterization of Nanometer-Size Fe3O4 and γ-Fe2O3 Particles
Y. Kang (1996)
Hydroxyapatite/Hydroxyapatite‐Whisker Composites without Sintering Additives: Mechanical Properties and Microstructural Evolution
W. Suchanek (1997)
Magnetic field strength requirements to capture superparamagnetic nanoparticles within capillary flow
B. Hallmark (2010)
Optimization of surface micromorphology for enhanced osteoblast responses in vitro.
K. T. Bowers (1992)
The selective estrogen receptor modulator raloxifene regulates osteoclast and osteoblast activity in vitro.
A. Taranta (2002)
Towards dynamic control of magnetic fields to focus magnetic carriers to targets deep inside the body.
B. Shapiro (2009)
Osteoblast adhesion on nanophase ceramics.
T. Webster (1999)
Giant osteoclast formation and long-term oral bisphosphonate therapy.
J. C. Roos (2009)
Bisphosphonates: Mechanisms of Action
H. Fleisch (1998)
Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications.
A. Gupta (2005)
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Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite.
Jazmín I González Ocampo (2018)
Magnetic composite scaffolds of polycaprolactone/nFeHA, for bone-tissue engineering
E. Díaz (2016)
Development and characterisation of novel Ce-doped hydroxyapatite–Fe3O4 nanocomposites and their in vitro biological evaluations for biomedical applications
P. Baskaran (2018)
Biomimetic and mesoporous nano-hydroxyapatite for bone tissue application: a short review.
Maria Chiara Palmieri (2019)
Iron doped β-Tricalcium phosphate: Synthesis, characterization, hyperthermia effect, biocompatibility and mechanical evaluation.
R. Singh (2017)
Structural elucidation and iron oxidation states in situ formed β‐Ca3(PO4)2/α‐Fe2O3 composites
P. N. Kumar (2017)
In vivo evaluation of a novel nanocomposite porous 3D scaffold in a rabbit model: histological analysis
S. K. Mahmood (2017)
Targeted superparamagnetic nanoparticles coated with 2-deoxy-d-gloucose and doxorubicin more sensitize breast cancer cells to ionizing radiation.
J. Pirayesh Islamian (2017)
Ion substitution in biological and synthetic apatites
A. Bigi (2016)
Nanotechnology in bone tissue engineering.
G. Walmsley (2015)
Nanosized biomaterials for regenerative medicine
R. Conte (2018)
Magnetic field assisted stem cell differentiation - role of substrate magnetization in osteogenesis.
Sunil Kumar Boda (2015)
Recent progress on fabrication and drug delivery applications of nanostructured hydroxyapatite.
S. Mondal (2018)
Development and biocompatibility tests of electrospun poly-l-lactide nanofibrous membranes incorporating oleic acid-coated Fe3O4
H. Wang (2013)
Nanoparticles and their potential for application in bone
A. Tautzenberger (2012)
Advances in Nanotechnology for the Treatment of Osteoporosis
Mikayla E. Barry (2016)
Clinical, Radiological, and Histological Assessment of Magnetic Nanoparticles as Pulpotomy Medicament in Primary Molars
Harivinder R Konyala (2018)
In vitro cytotoxicity of iron oxide nanoparticles: Effects of chitosan and polyvinyl alcohol as stabilizing agents
P. Tran (2018)
Bingyun Li (2018)
Understanding magnetic nanoparticle osteoblast receptor-mediated endocytosis using experiments and modeling.
N. Tran (2013)
Fluorapatite coated iron oxide nanostructure for biomedical applications
S. Karthi (2017)
Substituted hydroxyapatites for bone regeneration: A review of current trends.
J. Ratnayake (2017)
Microemulsion synthesis and magnetic properties of hydroxyapatite-encapsulated nano CoFe2O4
F. Foroughi (2015)
Magnetic hydroxyapatite: a promising multifunctional platform for nanomedicine application
S. Mondal (2017)
Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
Sifeng Shi (2012)
Synthetic Study and Merits of Fe3O4 Nanoparticles as Emerging Material
S. Jamil (2017)
Size effect of nano-hydroxyapatite on proliferation of odontoblast-like MDPC-23 cells.
N. Li (2019)
Synthesis of one-dimensional magnetite hydroxyapatite nanorods on reduced graphene oxide sheets for selective separation and controlled delivery of hemoglobin
G. Bharath (2020)
Biomineralization Process in Hard Tissues: The Interaction Complexity within Protein and Inorganic Counterparts.
Vaibhav Sharma (2020)
Highly efficient mesenchymal stem cell proliferation on poly-ε-caprolactone nanofibers with embedded magnetic nanoparticles
J. Daňková (2015)
Biocompatible nanocomposite of TiO2 incorporated bi-polymer for articular cartilage tissue regeneration: A facile material.
L. Cao (2018)
Nickel hydroxide/hydroxyapatite nanorods as affinity adsorbents for separation histidine-tagged proteins
Shasha Yao (2015)See more