← Back to Search
Fibrin-based Scaffold Incorporating VEGF- And BFGF-loaded Nanoparticles Stimulates Wound Healing In Diabetic Mice.
P. Losi, E. Briganti, C. Errico, A. Lisella, E. Sanguinetti, F. Chiellini, G. Soldani
Published 2013 · Materials Science, Medicine
Download PDFAnalyze on Scholarcy
Diabetic skin ulcers are difficult to heal spontaneously due to the reduced levels and activity of endogenous growth factors. Recombinant human vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are known to stimulate cell proliferation and accelerate wound healing. Direct delivery of VEGF and bFGF at the wound site in a sustained and controllable way without loss of bioactivity would enhance their biological effects. The aim of this study was to develop a poly(ether)urethane-polydimethylsiloxane/fibrin-based scaffold containing poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with VEGF and bFGF (scaffold/GF-loaded NPs) and to evaluate its wound healing properties in genetically diabetic mice (db/db). The scaffold application on full-thickness dorsal skin wounds significantly accelerated wound closure at day 15 compared to scaffolds without growth factors (control scaffold) or containing unloaded PLGA nanoparticles (scaffold/unloaded NPs). However, the closure rate was similar to that observed in mice treated with scaffolds containing free VEGF and bFGF (scaffold/GFs). Both scaffolds containing growth factors induced complete re-epithelialization, with enhanced granulation tissue formation/maturity and collagen deposition compared to the other groups, as revealed by histological analysis. The ability of the scaffold/GF-loaded NPs to promote wound healing in a diabetic mouse model suggests its potential use as a dressing in patients with diabetic foot ulcers.
This paper references
Nitric oxide-releasing nanoparticles accelerate wound healing by promoting fibroblast migration and collagen deposition.
G. Han (2012)
A composite fibrin-based scaffold for controlled delivery of bioactive pro-angiogenetic growth factors.
E. Briganti (2010)
Phase I trial on the safety of topical rhVEGF on chronic neuropathic diabetic foot ulcers.
J. Hanft (2008)
Cellular dysfunction in the diabetic fibroblast: impairment in migration, vascular endothelial growth factor production, and response to hypoxia.
O. Lerman (2003)
The molecular biology of chronic wounds and delayed healing in diabetes
R. Blakytny (2006)
Growth Factors and Chronic Wound Healing: Past, Present, and Future
R. Goldman (2004)
Pathophysiology of acute wound healing.
J. Li (2007)
Silicone based polyurethane materials: a promising biocompatible elastomeric formulation for cardiovascular applications
E. Briganti (2006)
Nanotechnology promotes the full‐thickness diabetic wound healing effect of recombinant human epidermal growth factor in diabetic rats
Y. Chu (2010)
Photocrosslinkable chitosan hydrogel containing fibroblast growth factor-2 stimulates wound healing in healing-impaired db/db mice.
K. Obara (2003)
Recombinant basic fibroblast growth factor accelerates wound healing.
G. McGee (1988)
Consensus development conference on diabetic foot wound care. 7-8 April 1999, Boston, MA. American Diabetes Association
Micro/nanostructured polymeric systems for biomedical and pharmaceutical applications.
F. Chiellini (2008)
Combined vascular endothelial growth factor-A and fibroblast growth factor 4 gene transfer improves wound healing in diabetic mice
A. Jaźwa (2010)
Current advances in research and clinical applications of PLGA-based nanotechnology
J. Lü (2009)
Design and characterisation of new nanoparticulate polymer blends for drug delivery
N. Csaba (2004)
Molecular and mechanistic validation of delayed healing rat wounds as a model for human chronic wounds
C. Chen (1999)
Tissue response to poly(ether)urethane-polydimethylsiloxane-fibrin composite scaffolds for controlled delivery of pro-angiogenic growth factors.
P. Losi (2010)
Biodegradable nanoparticles are excellent vehicle for site directed in-vivo delivery of drugs and vaccines
A. Mahapatro (2011)
Analysis of the acute and chronic wound environments: the role of proteases and their inhibitors
N. Trengove (1999)
Protein Instability in Poly(Lactic-co-Glycolic Acid) Microparticles
M. van de Weert (2004)
Global prevalence of diabetes: estimates for the year 2000 and projections for 2030.
S. Wild (2004)
Clinical efficacy of basic fibroblast growth factor (bFGF) for diabetic ulcer.
H. Uchi (2009)
A method for producing a device applicable to biological tissues, particularly a patch for treating damaged tissues, and a device obtained by said method
G Soldani (2010)
A fibrin gel loaded with chitosan nanoparticles for local delivery of rhEGF: preparation and in vitro release studies
W. Zhou (2011)
Protein-loaded PLGA–PEO blend nanoparticles: encapsulation, release and degradation characteristics
M. J. Santander-Ortega (2010)
Topical vascular endothelial growth factor accelerates diabetic wound healing through increased angiogenesis and by mobilizing and recruiting bone marrow-derived cells.
R. Galiano (2004)
Heparin Regulates Vascular Endothelial Growth Factor165-dependent Mitogenic Activity, Tube Formation, and Its Receptor Phosphorylation of Human Endothelial Cells
Satoko Ashikari-Hada (2005)
The molecular basis for impaired hypoxia-induced VEGF expression in diabetic tissues
Hariharan Thangarajah (2009)
PLGA nanoparticles prepared by nanoprecipitation: drug loading and release studies of a water soluble drug.
T. Govender (1999)
Nanoparticles based on PLGA:poloxamer blends for the delivery of proangiogenic growth factors.
I. d'Angelo (2010)
Regulation of Vascular Endothelial Growth Factor Expression in Cultured Keratinocytes.
S. Frank (1995)
Effects of vascular endothelial growth factor on wound closure rates in the genetically diabetic mouse model
Loren M. Kirchner (2003)
Nitric oxide-releasing nanoparticles accelerate wound healing in NOD-SCID mice.
Karin Blecher (2012)
Promotion of skin regeneration in diabetic rats by electrospun core-sheath fibers loaded with basic fibroblast growth factor.
Y. Yang (2011)
This paper is referenced by
Evaluation of dermal tissue regeneration using resveratrol loaded fibrous matrix in a preclinical mouse model of full‐thickness ischemic wound
Rajesh Lakshmanan (2019)
Nanoparticle-Based Therapeutic Approach for Diabetic Wound Healing
Hariharan Ezhilarasu (2020)
The preparation of the ordered pores colloidal crystal scaffold and its role in promoting growth of lung cells.
Qing Ban (2019)
PLGA based drug delivery systems: Promising carriers for wound healing activity
K. K. Chereddy (2016)
Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing
Shixuan Chen (2015)
A novel cartilage tissue construction based on artificial cells and matrix-shaping
Yuangang Liu (2015)
How Signaling Molecules Regulate Tumor Microenvironment: Parallels to Wound Repair
P. Gál (2017)
Diabetic Foot Ulcer: An Update
M. Zubair (2021)
Nanomaterials as potential and versatile platform for next generation tissue engineering applications.
R. Singla (2019)
Nanotechnology approaches for skin wound regeneration using drug-delivery systems
Itxaso Garcia-Orue (2016)
Adenoviral-mediated gene transfer of insulin-like growth factor 1 enhances wound healing and induces angiogenesis.
Swathi Balaji (2014)
Chitosan-hyaluronic acid/VEGF loaded fibrin nanoparticles composite sponges for enhancing angiogenesis in wounds.
A. Mohandas (2015)
Nanotechnology-driven advances in the treatment of diabetic wounds.
H. Zare (2020)
Novel biomaterial strategies for controlled growth factor delivery for biomedical applications
Zhenming Wang (2017)
Dual growth factor delivery using PLGA nanoparticles in silk fibroin/PEGDMA hydrogels for articular cartilage tissue engineering.
Milad Fathi-Achachelouei (2019)
Local delivery of growth factors using wound dressings
O. Catanzano (2020)
Sequential Delivery of Cryogel Released Growth Factors and Cytokines Accelerates Wound Healing and Improves Tissue Regeneration
S. Jimi (2020)
Platelet-Rich Plasma Derived Growth Factors Contribute to Stem Cell Differentiation in Musculoskeletal Regeneration
Y. Qian (2017)
Dermal Regeneration and Induction of Wound Closure in Diabetic Wounds
G. Giatsidis (2018)
Molecular systems for targeted delivery and controlled release of growth factors for chronic wound treatment
A. R. Lipunov (2020)
miRNA-221-3p in Endothelial Progenitor Cell-Derived Exosomes Accelerates Skin Wound Healing in Diabetic Mice
Juan Xu (2020)
Flexible electrical stimulation device with Chitosan-Vaseline® dressing accelerates wound healing in diabetes
Xiao-Feng Wang (2021)
Does survivin overexpression enhance the efficiency of fibroblast cell-based wound therapy?
Fereshteh Shojaei-Ghahrizjani (2020)
Do Mesenchymal Stem Cells Have a Role to Play in Cutaneous Wound Healing
I. Pountos (2014)
Antimicrobial peptide modification enhances the gene delivery and bactericidal efficiency of gold nanoparticles for accelerating diabetic wound healing.
S. Wang (2018)
Mesenchymal Stem Cells Coated by the Extracellular Matrix Promote Wound Healing in Diabetic Rats
L. Wang (2019)
Nanomedicines and gene therapy for the delivery of growth factors to improve perfusion and oxygenation in wound healing
C. Desmet (2018)
Silica nanoparticles as gene delivery systems for skin tissue repair
Xiaolin Wang (2015)
Role of Growth Factors in the Treatment of Diabetic Foot Ulceration
Deepti Singh (2021)
The dual delivery of growth factors and antimicrobial peptide by PLGA/GO composite biofilms to promote skin-wound healing
Ziyan Zhang (2020)
Nanophytomedicines for the Prevention of Metabolic Syndrome: A Pharmacological and Biopharmaceutical Review
Zeinab Nouri (2020)
Enhancement of Skin Wound Healing by rhEGF-Loaded Carboxymethyl Chitosan Nanoparticles
P. Zhang (2020)See more