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Preparation And Characterization Of A Novel Tobramycin-containing Antibacterial Collagen Film For Corneal Tissue Engineering.
Y. Liu, L. Ren, Kai Long, L. Wang, Y. Wang
Published 2014 · Materials Science, Medicine
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Corneal disease is a major cause of blindness and keratoplasty is an effective treatment method. However, clinical treatment is limited due to a severe shortage of high-quality allogeneic corneal tissues and the bacterial infection after corneal transplantation. In this study, we develop a novel artificial and antibacterial collagen film (called Col-Tob) for corneal repair. In the Col-Tob film, the tobramycin, which is an aminoglycoside antibiotic to treat various types of bacterial infections, was cross-linked by 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide onto the collagen. Physical properties, antibacterial property and biocompatibility of the films were characterized. The results indicate that the film is basically transparent and has appropriate mechanical properties. Cell experiments show that human corneal epithelial cells could adhere to and proliferate well on the film. Most importantly, the film exhibits excellent antibacterial effect in vitro. Lamellar keratoplasty shows that the Col-Tob film can be sutured in rabbit eyes and are epithelialized completely in 15 ± 5 days, and their transparency is restored quickly in the first month. Corneal rejection reaction, neovascularization and keratoconus are not observed within 3 months. This film, which can be prepared in large quantities and at low cost,should have potential application in corneal repair.
This paper references
The spectrum of antimicrobial peptide expression at the ocular surface.
R. Mcintosh (2005)
Systemic exposure to tobramycin after local antibiotic treatment with calcium sulphate as carrier material
P. Wahl (2010)
Light transmittance of the human cornea from 320 to 700 nm for different ages
T. Berg (1994)
Host Defense at the Ocular Surface
E. Pearlman (2013)
The Treatment of Chronic Osteomyelitis with a Biodegradable Antibiotic-Impregnated Implant
S. Gitelis (2002)
A dual-layer silk fibroin scaffold for reconstructing the human corneal limbus.
Laura J Bray (2012)
Nanocrystalline hydroxyapatite and calcium sulphate as biodegradable composite carrier material for local delivery of antibiotics in bone infections.
M. Rauschmann (2005)
Twenty-five–Year Panorama of Corneal Immunology: Emerging Concepts in the Immunopathogenesis of Microbial Keratitis, Peripheral Ulcerative Keratitis, and Corneal Transplant Rejection
M. R. Dana (2000)
PEGstabilized carbodiimide crosslinked collagen–chitosan hydrogels for corneal tissue
M Rafat (2008)
Fibrillar structure and mechanical properties of collagen.
P. Fratzl (1998)
Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes.
W. Liu (2009)
Effects of collagen cross-linking on the interlamellar cohesive strength of porcine cornea.
Chen Tao (2013)
The use of phospholipase A(2) to prepare acellular porcine corneal stroma as a tissue engineering scaffold.
Z. Wu (2009)
Structure and properties of collagen vitrigel membranes for ocular repair and regeneration applications.
X. Calderón-Colón (2012)
In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating.
W. Chen (2006)
Application of multiplayer HA / collagen scaffold in cartilage tissue engineering
G Wu (2007)
Application of Multilayer Collagen/HA Scaffold in Cartilage Tissue Engineering
G. Wu (2007)
Application of multiplayer HA/collagen scaffold in cartilage tissue engineering. Key Eng Mater 2007;2:1549–52
G Wu (2007)
Establishment of a corneal epithelial cell line spontaneously derived from human limbal cells.
J. Liu (2007)
Chitosan-based thermosensitive hydrogel as a promising ocular drug delivery system: Preparation, characterization, and in vivo evaluation
Xingwei Chen (2012)
In vitro tobramycin elution analysis from a novel beta-tricalcium phosphate-silicate-xerogel biodegradable drug-delivery system.
M. Dicicco (2004)
Bombyx mori silk fibroin membranes as potential substrata for epithelial constructs used in the management of ocular surface disorders.
T. Chirila (2008)
A Biosynthetic Alternative to Human Donor Tissue for Inducing Corneal Regeneration: 24-Month Follow-Up of a Phase 1 Clinical Study
P. Fagerholm (2010)
A controlled-release ocular delivery system for ibuprofen based on nanostructured lipid carriers.
X. Li (2008)
PEG-stabilized carbodiimide crosslinked collagen-chitosan hydrogels for corneal tissue engineering.
M. Rafat (2008)
Comprehensive, Quantitative Mapping of T Cell Epitopes in Gluten in Celiac Disease
J. Tye-Din (2010)
Detection of specific collagen types in normal and keratoconus corneas.
D. A. Newsome (1981)
Twenty - five - year panorama of
Corneal repair by human corneal keratocyte-reprogrammed iPSCs and amphiphatic carboxymethyl-hexanoyl chitosan hydrogel.
Y. Chien (2012)
Effects of Collagen Cross-Linking on the Interlamellar Cohesive Strength of Porcine Cornea
Chen Tao (2013)
Silk fibroin in ocular tissue reconstruction.
D. Harkin (2011)
Bioengineering endothelialized neo-corneas using donor-derived corneal endothelial cells and decellularized corneal stroma.
Jin San Choi (2010)
Using Basement Membrane of Human Amniotic Membrane as a Cell Carrier for Cultivated Cat Corneal Endothelial Cell Transplantation
Wu Wen-can (2007)
Collagen films with suitable physical properties and biocompatibility for corneal tissue engineering prepared by ion leaching technique
Y. Liu (2012)
This paper is referenced by
Dry vs. wet: Properties and performance of collagen films. Part I. Mechanical behaviour and strain-rate effect.
Shirsha Bose (2020)
Layered nanofiber sponge with an improved capacity for promoting blood coagulation and wound healing.
K. Zhang (2019)
Microsphere - encapsulated astrocytes expressing Ngf promote axonal growth
L. Yao (2016)
An antibacterial collagen membrane crosslinked by the inclusion complex of β-cyclodextrin dialdehyde and ofloxacin for bacterial keratitis
Yawei Chen (2018)
To prepare the collagen‐based artificial cornea with improved mechanical and biological property by ultraviolet‐A/riboflavin crosslinking
Xuan Zhao (2017)
Construction and Evaluation of Collagen-Based Corneal Grafts Using Polycaprolactone To Improve Tension Stress
Xiaomin Sun (2020)
pH-responsive collagen fibrillogenesis in confined droplets induced by vapour diffusion
G. B. Ramirez-Rodríguez (2014)
Gentamicin modified chitosan film with improved antibacterial property and cell biocompatibility.
Y. Liu (2017)
Study on Silk/Carbamate Films
Hua Fu (2017)
Silk fibroin composite membranes for application in corneal regeneration
Shanshan Zhang (2015)
Modification of collagen-chitosan matrix by the natural crosslinker alginate dialdehyde.
Tianming Du (2016)
Using oxidized amylose as carrier of linalool for the development of antibacterial wound dressing.
Yongbo Lyu (2017)
Preparation of collagen/cellulose nanocrystals composite films and their potential applications in corneal repair
Lanfeng Qin (2020)
ESSI NIEMI HYDROGELS FOR 3D CULTURE OF HUMAN CORNEAL CELLS AIMING FOR TISSUE ENGINEERED CORNEAL APPLICATION
A. Mikhailova (2016)
Polyurethane Biomaterial-based Strategies toward Anti-infective Gingival Tissue Constructs
Meghan E. E. Wright (2019)
Natural-based nanocomposites for bone tissue engineering and regenerative medicine: a review.
S. Piña (2015)
Soft tissue engineering and microbial infections
M. Chifiriuc (2016)
PVA/anionic collagen membranes as drug carriers of ciprofloxacin hydrochloride with sustained antibacterial activity and potential use in the treatment of ulcerative keratitis
J. Daza (2020)
Improving the moisturizing properties of collagen film by surface grafting of chondroitin sulfate for corneal tissue engineering
Y. Liu (2016)
Light-triggered release of ciprofloxacin from an in situ forming click hydrogel for antibacterial wound dressings.
Yue Shi (2015)
Dry vs. wet: Properties and performance of collagen films. Part II. Cyclic and time-dependent behaviours.
Shirsha Bose (2020)
Improving the mechanical properties of collagen-based membranes using silk fibroin for corneal tissue engineering.
Kai Long (2015)
Biomimetic Corneal Stroma Using Electro-Compacted Collagen.
Z. Chen (2020)
A collagen film with micro-rough surface can promote the corneal epithelization process for corneal repair.
Y. Liu (2019)
Animal-derived natural products review: focus on novel modifications and applications.
Qianqian Fan (2015)
Bioadaptability: An Innovative Concept for Biomaterials
Y. Wang (2016)
Role of corneal collagen fibrils in corneal disorders and related pathological conditions.
H. Zhou (2017)
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Extracellular matrix scaffold crosslinked with vancomycin for multifunctional antibacterial bone infection therapy.
B. Fang (2020)
2.15 Collagen: Materials Analysis and Implant Uses☆
A. Sorushanova (2017)
Novel Collagen-based Scaffolds For Hollow Organ Regeneration
H. Hoogenkamp (2015)
Collagen membranes crosslinked by β-cyclodextrin polyrotaxane monoaldehyde with good biocompatibilities and repair capabilities for cornea repair
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