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Cytotoxic Effect And Apoptosis Induction By Silver Nanoparticles In HeLa Cells.

N. Miura, Y. Shinohara
Published 2009 · Chemistry, Medicine

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Nanosilver has well-known antibacterial properties, and is widely used in daily life as various medical and general products. In comparison with silver ion, there is serious lacking of information concerning the biological effects of nanoAg. In this study, we observed the cytotoxic effect of nanoAg in HeLa cells. The nanoAg-induced cytotoxicity was lower than that of AgNO(3), used as a silver ion source. Apoptosis evaluated by flowcytometric analysis was associated with this cell death. Further, the expressions of ho-1 and mt-2A, well-known oxidative stress-related genes, were up-regulated by nanoAg treatment. Our results showed that nanoAg possesses the potential for cytotoxicity, therefore, in the case of exposure at high concentrations, we should consider to protect from nanoAg-induced toxicity.
This paper references
10.1089/SUR.2006.032
In vitro analysis of a nanocrystalline silver-coated surgical mesh.
M. Cohen (2007)
10.1021/PR0504079
Proteomic analysis of the mode of antibacterial action of silver nanoparticles.
Chun-Nam Lok (2006)
Oxygen free radicals and metallothionein , Free Radic
M. Sato (1993)
10.2174/0929867043365080
Heme oxygenase-1: a novel therapeutic target in oxidative tissue injuries.
T. Takahashi (2004)
Mitochondria
G. Pappas (1959)
10.1002/JBM.A.30819
Construction of antibacterial multilayer films containing nanosilver via layer-by-layer assembly of heparin and chitosan-silver ions complex.
Jinhong Fu (2006)
10.1016/J.JCIS.2004.02.012
Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria.
I. Sondi (2004)
10.2131/JTS.31.391
Characterization of an immortalized hepatic stellate cell line established from metallothionein-null mice.
N. Miura (2006)
10.1016/0891-5849(93)90029-T
Oxygen free radicals and metallothionein.
M. Sato (1993)
10.1016/j.jtemb.2008.08.002
Metals and apoptosis: recent developments.
S. V. Rana (2008)
10.1166/JNN.2008.1193
Fabrication of biodegradable electrospun poly(L-lactide-co-glycolide) fibers with antimicrobial nanosilver particles.
X. Xu (2008)
10.1016/j.toxlet.2008.04.015
The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells.
Yi-Hong Hsin (2008)
10.1007/s10495-007-0756-2
Mitochondria, oxidative stress and cell death
M. Ott (2007)
10.1021/ES7032718
Nanoparticle silver released into water from commercially available sock fabrics.
T. Benn (2008)
10.1021/JA061442Z
Silver bromide nanoparticle/polymer composites: dual action tunable antimicrobial materials.
V. Sambhy (2006)
10.1021/ES8026314
Nanosilver toxicity: ions, nanoparticles--or both?
N. Lubick (2008)
10.1166/JNN.2007.737
Functional finishing of cotton fabrics using silver nanoparticles.
N. Vigneshwaran (2007)
10.1093/TOXSCI/KFI256
In vitro cytotoxicity of nanoparticles in mammalian germline stem cells.
L. Braydich-Stolle (2005)
10.1007/s10529-008-9786-2
Comparison of acute responses of mice livers to short-term exposure to nano-sized or micro-sized silver particles
Kyungeun Cha (2008)
10.1021/ES801785M
Toxicity of silver nanoparticles to Chlamydomonas reinhardtii.
E. Navarro (2008)
10.1126/SCIENCE.1114397
Toxic Potential of Materials at the Nanolevel
A. Nel (2006)
10.1128/AEM.69.7.4278-4281.2003
Mode of Bactericidal Action of Silver Zeolite and Its Comparison with That of Silver Nitrate
Y. Matsumura (2003)
10.1016/0147-619X(92)90008-X
Germanium and silver resistance, accumulation, and toxicity in microorganisms.
R. Slawson (1992)
10.1016/S0024-3205(01)01294-2
Specific induction of metallothionein synthesis by mitochondrial oxidative stress.
M. Kondoh (2001)
Salopek - Sondi , Silver nanoparticles as antimicrobial agent : a case study on E . coli as a model for Gram - negative bacteria
B. I. Sondi (2004)



This paper is referenced by
10.21608/blj.2017.47605
Apoptotic effect of silver nanoparticles in rat
I. Ashour (2017)
Toxicological Assessments of Released Engineered Nanoparticles from Aerosolized Products on Human Small Airway and Bronchial Epithelial Cells
K. Pearce (2018)
10.1007/s11356-015-5497-0
Nanoparticle pollution and associated increasing potential risks on environment and human health: a case study of China
Yang Gao (2015)
10.5012/BKCS.2012.33.10.3265
Comparative Toxicity Studies of Ultra-Pure Ag, Au, Co, and Cu Nanoparticles Generated by Laser Ablation in Biocompatible Aqueous Solution
Yea Seul Kim (2012)
Evaluation of Protective Effect of Penicillamine on Silver Nanoparticles-Induced Oxidative Stress in BALB/c Mice
F. Namazi (2015)
10.1016/J.PROCBIO.2011.11.003
Cytotoxic effect of Green synthesized silver nanoparticles using Melia azedarach against in vitro HeLa cell lines and lymphoma mice model
R. Sukirtha (2012)
10.1002/mbo3.698
Antibacterial effects and resistance induction of silver and gold nanoparticles against Staphylococcus aureus‐induced mastitis and the potential toxicity in rats
A. Elbehiry (2019)
10.2147/BCTT.S130952
Mentha arvensis (Linn.)-mediated green silver nanoparticles trigger caspase 9-dependent cell death in MCF7 and MDA-MB-231 cells
Prajna Paramita Banerjee (2017)
10.1208/s12249-019-1350-y
Effective Topical Delivery of H-AgNPs for Eradication of Klebsiella pneumoniae–Induced Burn Wound Infection
S. Chhibber (2019)
10.3109/17435390.2010.529176
Genotoxic analysis of silver nanoparticles in Drosophila
E. Demir (2011)
10.1088/2053-1591/AAF2D2
PEGylated silver nanoparticles from Sesbania aegyptiaca exhibit immunomodulatory and anti-cancer activity
Sureshbabu Ram Kumar Pandian (2018)
10.5772/60707
In vivo Assessment of Nanomaterials Toxicity
S. Clichici (2015)
Biological effects of selected metal nanoparticles in zebrafish (Danio rerio)
Olivia J. Osborne (2013)
10.1002/adhm.201700973
Moldable Hyaluronan Hydrogel Enabled by Dynamic Metal-Bisphosphonate Coordination Chemistry for Wound Healing.
L. Shi (2018)
10.21767/2581-804X.100009
Exposure to Silver Nanoparticles Affects Biofilm Structure and Adhesiveness
Holger Schmidt (2017)
10.1007/s00216-011-5266-y
Measuring silver nanoparticle dissolution in complex biological and environmental matrices using UV–visible absorbance
Justin M. Zook (2011)
10.1039/C2RA23131J
Revealing silver cytotoxicity using Au nanorods/Ag shell nanostructures: disrupting cell membrane and causing apoptosis through oxidative damage
Xuelian Cheng (2013)
Antimicrobial efficacy of nanosilver, sodium hypochlorite and chlorhexidine gluconate against Enterococcus faecalis
Mehrdad Lotfi (2011)
10.1007/s11356-014-3735-5
Synthesis and larvicidal activity of low-temperature stable silver nanoparticles from psychrotolerant Pseudomonas mandelii
A. Mageswari (2014)
10.1007/s13273-020-00100-x
Capture and toxicity assessment of Ag citrate nanoparticles using jellyfish extract
Jiwon E. Ha (2020)
10.1038/s41598-018-37645-w
Electronic origin of antimicrobial activity owing to surface effect
N. Miyazawa (2019)
10.12983/IJSRK-2014-P0182-0188
The Effect of different Concentrations of Silver Nanoparticles on Enzyme Activity and Liver Tissue of Adult Male Wistar Rats in-vivo Condition
Shahin Gavanji (2014)
Cytotoxicity and Cell Cycle Effects of Gold and Silver Nanoparticles in Human Intestinal Epithelial Caco-2 Cells
Mariana Vinhas Fernandes (2014)
10.13040/ijpsr.0975-8232.10(10).4587-97
THE CORCHORUS HIRSUTUS PLANT MATERIAL SELECTED FOR THE SYNTHESIS OF NANOSILVER Optimized Synthesis of Nanosilver : Optimized
D. Ashok (2019)
10.1007/s11426-018-9376-7
Value-added anticancer reactivity of sub-5 nm Ag-drug nanoparticles derived from organosilver(I) MOF
Chao-yu Song (2018)
Designing an antimicrobial and cell-adhesive multilayer via plasma polymerization
A. Lotz (2012)
Bioreactivity of ZnO and Ag nanostructures in the lung
I. Theodorou (2015)
10.1016/j.tiv.2016.03.004
Toxicological evaluation of representative silver nanoparticles in macrophages and epithelial cells.
K. Nguyen (2016)
10.1111/j.1750-3841.2011.02222.x
Characterization and evaluation of the Ag+-loaded soy protein isolate-based bactericidal film-forming dispersion and films.
Qingshen Sun (2011)
10.1155/2017/5107485
Heterogeneous Responses of Ovarian Cancer Cells to Silver Nanoparticles as a Single Agent and in Combination with Cisplatin
Cale D Fahrenholtz (2017)
10.1111/j.1742-7843.2010.00668.x
Metallic silver fragments cause massive tissue loss in the mouse brain.
L. Locht (2011)
10.1007/S11051-010-9944-Z
Synthesis and characterization of bovine femur bone hydroxyapatite containing silver nanoparticles for the biomedical applications
R. Nirmala (2011)
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