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Addressing The Problem Of Cationic Lipid-mediated Toxicity: The Magnetoliposome Model.

S. Soenen, A. Brisson, M. de Cuyper
Published 2009 · Chemistry, Medicine
Referenced 2 times by Citationsy Users

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The high biocompatibility and versatile nature of liposomes made these particles keystone components in many hot-topic research areas. For transfection and cell labelling purposes, synthetic cationic lipids are often added, but in most studies, little attention has been paid to their cytotoxic effects. In the present work, cationic magnetoliposomes (MLs), i.e. iron oxide cores enwrapped by a phospholipid bilayer (dimyristoylphosphatidylcholine or sphingomyelin) doped with cationic lipids (1,2-distearoyl-3-trimethylammonium propane), serve as a model to examine cationic lipid toxicity. Mechanisms of cytotoxic effects were found to be either dependent or independent of actual particle internalisation according to data obtained in the absence or presence of several endocytosis inhibitors. The former seem to be caused by the generation of reactive oxygen species (ROS) leading to a Ca2+ influx at high ROS levels. The latter are due to a destabilisation of the cell plasma membrane upon transfer of the cationic lipid from the ML bilayer into the plasma membrane. However, these adverse effects can be diminished by the use of a ROS scavenger, a Ca(2+)-channel blocker or by modulating the liposome size, lipid bilayer constitution or by stabilising the membrane by anchoring it on a solid core. Careful attention must be paid in terms of assessing cell viability as the effects are highly time dependent and the data suggest the incompatibility of using the well-known MTT assay when high levels of ROS species are generated.
This paper references
10.1016/S0006-2952(03)00240-5
The mitochondrial uncoupler dicumarol disrupts the MTT assay.
A. Collier (2003)
10.1016/j.jconrel.2008.05.005
Reactive oxygen species play a central role in the activity of cationic liposome based cancer vaccine.
Weili Yan (2008)
Lysosomalredox-active iron is important for oxidative stress-induced DNA damage
T Kurz (2004)
10.1016/J.BIOMATERIALS.2006.03.006
The drug encapsulation efficiency, in vitro drug release, cellular uptake and cytotoxicity of paclitaxel-loaded poly(lactide)-tocopheryl polyethylene glycol succinate nanoparticles.
Z. Zhang (2006)
10.1002/(SICI)1097-0290(19960320)49:6<654::AID-BIT6>3.0.CO;2-N
Catalytic durability of magnetoproteoliposomes captured by high‐gradient magnetic forces in a miniature fixed‐bed reactor
M. de Cuyper (1996)
10.1038/nn1380
Endocytosis-dependent desensitization and protein synthesis–dependent resensitization in retinal growth cone adaptation
M. Piper (2005)
10.1073/pnas.0435906100
Cell transfection in vitro and in vivo with nontoxic TAT peptide-liposome–DNA complexes
V. Torchilin (2003)
10.1023/A:1007474912498
Inhibition by singlet oxygen quenchers of oxidative damage to DNA produced in cultured cells by exposure to a quinolone antibiotic and ultraviolet A irradiation
L. Verna (2004)
10.1016/J.JCONREL.2006.04.014
Toxicity of cationic lipids and cationic polymers in gene delivery.
Hongtao Lv (2006)
10.1111/j.1349-7006.2006.00382.x
4‐S‐Cysteaminylphenol‐loaded magnetite cationic liposomes for combination therapy of hyperthermia with chemotherapy against malignant melanoma
A. Ito (2007)
10.1263/JBB.100.1
Medical application of functionalized magnetic nanoparticles.
A. Ito (2005)
10.1007/s11095-005-2496-8
Lyophilized Paclitaxel Magnetoliposomes as a Potential Drug Delivery System for Breast Carcinoma via Parenteral Administration: In Vitro and in Vivo Studies
J. Zhang (2005)
Bilayer curvature effects on the non-protein supported transfer of phospholipids
M De Cuyper (1986)
10.1016/j.biomaterials.2008.08.001
Mechanisms of unmodified CdSe quantum dot-induced elevation of cytoplasmic calcium levels in primary cultures of rat hippocampal neurons.
Mingliang Tang (2008)
10.1196/ANNALS.1297.048
Lysosomal Redox‐Active Iron Is Important for Oxidative Stress‐Induced DNA Damage
T. Kurz (2004)
10.1016/S0005-2736(97)00126-0
Toxicity and immunomodulatory activity of liposomal vectors formulated with cationic lipids toward immune effector cells.
M. Filion (1997)
10.1016/J.DIABRES.2006.12.008
Determination of the antioxidant status of plasma from type 2 diabetic patients.
L. Medina (2007)
10.2217/17435889.3.6.761
Co-delivery of siRNA and an anticancer drug for treatment of multidrug-resistant cancer.
M. Saad (2008)
10.2217/17435889.2.1.85
Liposome-nanoparticle hybrids for multimodal diagnostic and therapeutic applications.
W. T. Al-Jamal (2007)
10.1148/RADIOL.2392042110
Magnetic targeting of magnetoliposomes to solid tumors with MR imaging monitoring in mice: feasibility.
Jean-Paul Fortin-Ripoche (2006)
10.1002/cbic.200700327
Optimal Conditions for Labelling of 3T3 Fibroblasts with Magnetoliposomes without Affecting Cellular Viability
S. Soenen (2007)
10.1016/J.FREERADBIOMED.2004.01.016
Iron oxide particles for molecular magnetic resonance imaging cause transient oxidative stress in rat macrophages.
Albrecht Stroh (2004)
10.1016/J.BBRC.2006.01.129
Cerium and yttrium oxide nanoparticles are neuroprotective.
D. Schubert (2006)
10.1002/smll.200701043
Functionalized-quantum-dot-liposome hybrids as multimodal nanoparticles for cancer.
W. T. Al-Jamal (2008)
10.1152/AJPLUNG.2000.279.5.L878
Free radical-mediated transgene inactivation of macrophages by endotoxin.
S. Dokka (2000)
10.1016/S0076-6879(04)86013-0
Preparation of magnetically labeled cells for cell tracking by magnetic resonance imaging.
J. Bulte (2004)
10.1016/J.JCONREL.2004.08.007
Tumor-targeted liposomes: doxorubicin-loaded long-circulating liposomes modified with anti-cancer antibody.
A. Lukyanov (2004)
10.1097/00004647-200111000-00002
Effects of the Nitrone Radical Scavengers PBN and S-PBN on In vivo Trapping of Reactive Oxygen Species after Traumatic Brain Injury in Rats
N. Marklund (2001)
10.1016/j.biomaterials.2008.05.020
Cationic lipid bilayer coated gold nanoparticles-mediated transfection of mammalian cells.
Peicai Li (2008)
10.1007/s00262-007-0390-4
A simple but effective cancer vaccine consisting of an antigen and a cationic lipid
W. Chen (2007)
10.1016/J.JCONREL.2005.09.018
Recombinant polymers for cancer gene therapy: a minireview.
M. Haider (2005)
10.1002/smll.200800199
Assessing methods for blood cell cytotoxic responses to inorganic nanoparticles and nanoparticle aggregates.
B. Díaz (2008)
10.1016/0024-3205(77)90481-7
Liposomes as drug carriers.
J. H. Fendler (1977)
10.1023/A:1007504613351
Oxygen Radical-Mediated Pulmonary Toxicity Induced by Some Cationic Liposomes
Sujatha Dokka (2004)
10.1002/cbic.200700165
Molecular Effects of Uptake of Gold Nanoparticles in HeLa Cells
J. Khan (2007)
Bilayer curvature effects on the non-protein supported transfer of phospholipids
M De Cuyper (1986)
10.1016/J.BIOMATERIALS.2007.08.015
Interactions of antigen-loaded polylactide particles with macrophages and their correlation with the immune response.
V. Kanchan (2007)
10.1007/s11010-007-9653-9
Hydrogen peroxide activates calcium influx in human neutrophils
Miriam S. Giambelluca (2007)
10.1007/BF00256482
Magnetoliposomes. Formation and structural characterization.
M. de Cuyper (1988)
Magnetic targeting of magnetoliposomes to solid tumors with MR monitoring in mice: feasibility. Radiology 2005;239:415–24
JP Fortin-Ripoche (2005)
10.1002/cbic.200800510
Stable Long‐Term Intracellular Labelling with Fluorescently Tagged Cationic Magnetoliposomes
S. Soenen (2009)
10.1124/MOL.105.018408
Bafilomycin A1 Inhibits Chloroquine-Induced Death of Cerebellar Granule Neurons
J. Shacka (2006)
10.2217/17435889.4.2.177
Magnetoliposomes: versatile innovative nanocolloids for use in biotechnology and biomedicine.
S. Soenen (2009)
10.1046/j.1365-2141.1998.00834.x
Desferioxamine increases iron depletion and apoptosis induced by ara‐C of human myeloid leukaemic cells
A. Leardi (1998)
10.1016/J.YMTHE.2004.05.023
Macropinocytosis of polyplexes and recycling of plasmid via the clathrin-dependent pathway impair the transfection efficiency of human hepatocarcinoma cells.
C. Gonçalves (2004)
10.1290/1543-706X(2003)039<0329:ETOCCA>2.0.CO;2
Ergovaline toxicity on caco-2 cells as assessed by MTT, alamarblue, and DNA assays
N. Shappell (2003)
10.1016/J.CHEMPHYSLIP.2005.02.011
A method to evaluate the effect of liposome lipid composition on its interaction with the erythrocyte plasma membrane.
Joanna Wojewodzka (2005)
10.1016/j.biomaterials.2008.11.037
The effect of nocodazole on the transfection efficiency of lipid-bilayer coated gold nanoparticles.
D. Li (2009)



This paper is referenced by
10.1016/j.actbio.2016.11.045
Aminoclay as a highly effective cationic vehicle for enhancing adenovirus-mediated gene transfer through nanobiohybrid complex formation.
S. Kim (2017)
10.3109/1061186X.2011.628396
Dendrosome-dendriplex inside liposomes: as a gene delivery system
Sara Movassaghian (2011)
10.1002/cmmi.415
How to assess cytotoxicity of (iron oxide-based) nanoparticles: a technical note using cationic magnetoliposomes.
S. Soenen (2011)
10.1021/cr1003166
Effect of nanoparticles on the cell life cycle.
M. Mahmoudi (2011)
10.1155/2011/939851
A Review on Composite Liposomal Technologies for Specialized Drug Delivery
M. S. Mufamadi (2011)
10.1016/j.biomaterials.2010.08.075
Cytotoxic effects of iron oxide nanoparticles and implications for safety in cell labelling.
Stefaan J.H. Soenen (2011)
10.3109/17435390.2013.829589
Differential toxicological response to positively and negatively charged nanoparticles in the rat brain
K. Knudsen (2014)
10.1002/9781118610749.CH3
Challenges and Opportunities in Bringing RNAi Technologies from Bench to Bed
Sandesh Subramanya (2013)
10.1016/j.biomaterials.2011.08.075
The effect of static magnetic fields on the aggregation and cytotoxicity of magnetic nanoparticles.
Ji-Eun Bae (2011)
10.1016/B978-0-12-391858-1.00011-3
Investigating the toxic effects of iron oxide nanoparticles.
S. Soenen (2012)
An implantable nano-enabled bio-robotic intracranial device for targeted and prolonged drug delivery
M. S. Mufamadi (2015)
10.1016/j.nano.2014.08.004
In vivo toxicity of cationic micelles and liposomes.
K. Knudsen (2015)
10.1021/nn301714n
Cytotoxic effects of gold nanoparticles: a multiparametric study.
Stefaan J. Soenen (2012)
Injectable formulations forming an implant in situ as vehicle of silica microparticles embedding superparamagnetic iron oxide nanoparticles for the local, magnetically mediated hyperthermia treatment of solid tumors
Pol-Edern Le Renard (2011)
10.1021/nn403311c
Mechanisms of nanoparticle-mediated siRNA transfection by melittin-derived peptides.
K. Hou (2013)
10.1146/annurev-anchem-062011-143134
Assessing nanoparticle toxicity.
S. Love (2012)
10.1038/s41598-019-41122-3
p5RHH nanoparticle-mediated delivery of AXL siRNA inhibits metastasis of ovarian and uterine cancer cells in mouse xenografts
K. A. Mills (2019)
10.1002/smll.201000763
Intracellular nanoparticle coating stability determines nanoparticle diagnostics efficacy and cell functionality.
S. Soenen (2010)
10.1201/B17191-2
Introduction—Biointeractions of Nanomaterials: Challenges and Solutions
V. Sutariya (2014)
10.4155/tde.12.129
Magnetoliposomes and their potential in the intelligent drug-delivery field.
A. Bakandritsos (2012)
Nanostructured Carriers for Photodynamic Therapy Applications in microbiology
J. Longo (2011)
Novel siRNA lipoplexes : their targeted and untargeted delivery to mammalian cells in culture.
Shantal. Dorasamy (2011)
Formulations pour le traitement local de tumeurs solides par hyperthermie à médiation magnétique
Pol-Edern Le Renard (2011)
10.1016/j.carbpol.2016.07.099
Chitosan-coated microvesicles: Effect of polysaccharide-phospholipid affinity on decafluorobutane dissolution.
Guilherme F. Picheth (2016)
10.1016/J.TALANTA.2019.05.061
Magnetic nanostructures for preconcentration, speciation and determination of chromium ions: A review.
H. Filik (2019)
10.2174/1568026614666140329232757
Liposomes as nanovaccine delivery systems.
K. A. Ghaffar (2014)
The Development of a Dual-ligand PEGylated Liposome Nanotechnology for Cell-selective Targeted Vascular Gene Therapy
Fisher (2019)
10.13023/ETD.2019.121
TOWARDS THE RATIONAL DESIGN AND APPLICATION OF POLYMERS FOR GENE THERAPY: INTERNALIZATION AND INTRACELLULAR FATE
Landon Mott (2019)
10.1002/smll.200902084
High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling.
S. Soenen (2010)
10.1039/B911260J
Adsorption of plasmid DNA onto lipid/polymer particle assemblies
Anne-Lise Troutier-Thuilliez (2009)
10.1089/ten.TEA.2010.0539
Vascular endoluminal delivery of mesenchymal stem cells using acoustic radiation force.
C. Toma (2011)
10.1016/j.colsurfb.2019.110435
Magnetoliposomes of mixed biomimetic and inorganic magnetic nanoparticles as enhanced hyperthermia agents.
Ylenia Jabalera (2019)
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